Ophiocordyceps sinensis (strain Co18 / CGMCC 3.14243) (Yarsagumba caterpillar fungus) (Hirsutella sinensis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Ophiocordyceps; Ophiocordyceps sinensis

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6968 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T5AIZ2|T5AIZ2_OPHSC Uncharacterized protein OS=Ophiocordyceps sinensis (strain Co18 / CGMCC 3.14243) OX=911162 GN=OCS_01720 PE=4 SV=1
MM1 pKa = 6.73STYY4 pKa = 10.66NGRR7 pKa = 11.84HH8 pKa = 5.47GANASHH14 pKa = 6.85YY15 pKa = 10.73LRR17 pKa = 11.84DD18 pKa = 3.85LNTINPQDD26 pKa = 3.77TTTDD30 pKa = 3.37DD31 pKa = 3.52AFNIEE36 pKa = 4.67DD37 pKa = 4.5DD38 pKa = 4.18LALFTNTQFFDD49 pKa = 3.65FDD51 pKa = 3.8SGQNMDD57 pKa = 4.55FQAQPVKK64 pKa = 10.65PDD66 pKa = 3.18VDD68 pKa = 4.88DD69 pKa = 3.93VAQANSSPSGDD80 pKa = 3.75DD81 pKa = 3.05SAPPVMSDD89 pKa = 4.46LPNLDD94 pKa = 4.72FISGQLAASPSLVLSGNSCYY114 pKa = 10.55AGPVLHH120 pKa = 6.65LVSSIHH126 pKa = 6.79PSLLLFSSPCEE137 pKa = 3.99VLL139 pKa = 3.94

Molecular weight: 14.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T5A9U8|T5A9U8_OPHSC Cell division protein Cdc14 OS=Ophiocordyceps sinensis (strain Co18 / CGMCC 3.14243) OX=911162 GN=OCS_05705 PE=4 SV=1
MM1 pKa = 7.54LSGVRR6 pKa = 11.84PSGVRR11 pKa = 11.84PSGVRR16 pKa = 11.84PSGVRR21 pKa = 11.84PSGVRR26 pKa = 11.84PSGVRR31 pKa = 11.84PSGVRR36 pKa = 11.84PSGVRR41 pKa = 11.84PSGVRR46 pKa = 11.84PSGVRR51 pKa = 11.84PSGVRR56 pKa = 11.84PSGVRR61 pKa = 11.84PSGVRR66 pKa = 11.84PSGVRR71 pKa = 11.84PSGVRR76 pKa = 11.84PSGVRR81 pKa = 11.84PSGVRR86 pKa = 11.84PSGVRR91 pKa = 11.84GYY93 pKa = 11.45GPTRR97 pKa = 11.84SS98 pKa = 3.42

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6968

0

6968

3101960

49

5503

445.2

49.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.579 ± 0.031

1.322 ± 0.011

6.095 ± 0.021

5.914 ± 0.028

3.531 ± 0.018

7.26 ± 0.028

2.516 ± 0.013

4.079 ± 0.019

4.662 ± 0.026

9.028 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.318 ± 0.009

3.192 ± 0.015

6.148 ± 0.025

4.037 ± 0.024

6.831 ± 0.026

7.851 ± 0.034

5.425 ± 0.021

6.36 ± 0.025

1.421 ± 0.012

2.431 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski