Secundilactobacillus mixtipabuli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Secundilactobacillus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2292 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z5IA35|A0A1Z5IA35_9LACO Probable membrane transporter protein OS=Secundilactobacillus mixtipabuli OX=1435342 GN=IWT30_00591 PE=3 SV=1
MM1 pKa = 7.56FEE3 pKa = 4.33NNRR6 pKa = 11.84PRR8 pKa = 11.84YY9 pKa = 9.74ASFGIVNSLPGEE21 pKa = 4.37IIDD24 pKa = 4.04QIWYY28 pKa = 9.68IIDD31 pKa = 3.7NNLQGMFQLNEE42 pKa = 4.13IISFNLTNHH51 pKa = 5.98NNHH54 pKa = 6.21LTFDD58 pKa = 5.13FIQQEE63 pKa = 4.57SVVASFDD70 pKa = 3.61TPFPYY75 pKa = 10.59AEE77 pKa = 4.57SFPGSLWVYY86 pKa = 11.47DD87 pKa = 4.64DD88 pKa = 3.81GTSQIVMLPKK98 pKa = 10.72EE99 pKa = 4.25EE100 pKa = 4.07MM101 pKa = 3.76

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z5IE40|A0A1Z5IE40_9LACO Uncharacterized protein OS=Secundilactobacillus mixtipabuli OX=1435342 GN=IWT30_01898 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.59KK9 pKa = 7.38RR10 pKa = 11.84HH11 pKa = 5.81RR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.12GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.01VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2292

0

2292

711849

30

4350

310.6

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.362 ± 0.05

0.444 ± 0.011

5.939 ± 0.057

5.141 ± 0.066

4.065 ± 0.039

6.899 ± 0.071

2.28 ± 0.03

6.692 ± 0.046

6.078 ± 0.045

9.563 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.681 ± 0.028

4.731 ± 0.041

3.86 ± 0.038

4.836 ± 0.049

4.038 ± 0.055

5.984 ± 0.049

6.661 ± 0.093

7.28 ± 0.042

1.012 ± 0.019

3.452 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski