Bovine faeces associated smacovirus 5

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Drosmacovirus; Drosmacovirus bovas1

Average proteome isoelectric point is 5.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168MFL0|A0A168MFL0_9VIRU Putative capsid protein OS=Bovine faeces associated smacovirus 5 OX=1843753 PE=4 SV=1
MM1 pKa = 5.52VTVRR5 pKa = 11.84ISEE8 pKa = 4.33LYY10 pKa = 10.8DD11 pKa = 2.96MKK13 pKa = 11.2TEE15 pKa = 3.72IGKK18 pKa = 9.76IGLLGVHH25 pKa = 6.39TPTGSSIAKK34 pKa = 8.78RR35 pKa = 11.84WHH37 pKa = 5.99GLMINHH43 pKa = 7.04KK44 pKa = 9.25FMRR47 pKa = 11.84IKK49 pKa = 10.67SCDD52 pKa = 3.05IRR54 pKa = 11.84VACAFVLPADD64 pKa = 4.18PLQVGTDD71 pKa = 3.48TTDD74 pKa = 2.97EE75 pKa = 4.2GLIAPQDD82 pKa = 3.26IMNPILYY89 pKa = 9.83RR90 pKa = 11.84AVTNEE95 pKa = 3.03GWNTVINRR103 pKa = 11.84VYY105 pKa = 10.9SSAGMNYY112 pKa = 10.18DD113 pKa = 3.66VNSVLYY119 pKa = 8.18TTDD122 pKa = 3.17AFPSFSEE129 pKa = 4.43GVNDD133 pKa = 3.71LVYY136 pKa = 10.97YY137 pKa = 10.68GLLGQDD143 pKa = 3.91EE144 pKa = 4.58WRR146 pKa = 11.84KK147 pKa = 10.35AMPQAGLSMSGLRR160 pKa = 11.84PLVYY164 pKa = 10.0PLLSSYY170 pKa = 11.51GNTEE174 pKa = 3.06IMEE177 pKa = 4.5PSKK180 pKa = 10.32STSAISYY187 pKa = 9.35PPGQISGQDD196 pKa = 3.5GSSSNPGTNQRR207 pKa = 11.84VPVMRR212 pKa = 11.84GHH214 pKa = 7.18AVPMPRR220 pKa = 11.84LPCTTPGATTVDD232 pKa = 3.94TVPEE236 pKa = 4.14PVFTPQYY243 pKa = 9.64IPKK246 pKa = 9.8SYY248 pKa = 9.75VACIVMPPAKK258 pKa = 9.26MHH260 pKa = 6.34SLFFRR265 pKa = 11.84LAITWYY271 pKa = 10.09IEE273 pKa = 3.9FTDD276 pKa = 4.65LCTALDD282 pKa = 4.23KK283 pKa = 10.14EE284 pKa = 4.74TAGQVFDD291 pKa = 4.89AGDD294 pKa = 3.29YY295 pKa = 8.86TYY297 pKa = 11.2RR298 pKa = 11.84RR299 pKa = 11.84TYY301 pKa = 11.01SFTAGKK307 pKa = 9.23SLKK310 pKa = 10.72DD311 pKa = 3.26IDD313 pKa = 4.05DD314 pKa = 4.01TADD317 pKa = 3.41EE318 pKa = 4.54EE319 pKa = 4.66NSIDD323 pKa = 3.88SLNAPVHH330 pKa = 5.62LVVEE334 pKa = 4.75KK335 pKa = 11.05

Molecular weight: 36.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168MFL0|A0A168MFL0_9VIRU Putative capsid protein OS=Bovine faeces associated smacovirus 5 OX=1843753 PE=4 SV=1
MM1 pKa = 6.92VRR3 pKa = 11.84SHH5 pKa = 5.89NMEE8 pKa = 3.85RR9 pKa = 11.84ALPDD13 pKa = 3.18EE14 pKa = 5.18HH15 pKa = 6.83GTTQRR20 pKa = 11.84LAYY23 pKa = 9.58QPHH26 pKa = 6.48AKK28 pKa = 9.93EE29 pKa = 3.97GVIGEE34 pKa = 4.59EE35 pKa = 3.92ISPTSGKK42 pKa = 7.2THH44 pKa = 5.71YY45 pKa = 9.18QCKK48 pKa = 7.39WHH50 pKa = 6.82LSRR53 pKa = 11.84GEE55 pKa = 4.78SIDD58 pKa = 3.2GWKK61 pKa = 10.56LLIGPMGHH69 pKa = 6.4VDD71 pKa = 2.83IAVEE75 pKa = 4.13KK76 pKa = 10.56RR77 pKa = 11.84FTGYY81 pKa = 9.37EE82 pKa = 3.92EE83 pKa = 4.76KK84 pKa = 10.54DD85 pKa = 3.3GKK87 pKa = 9.25FVKK90 pKa = 9.89WPEE93 pKa = 3.88SPIAKK98 pKa = 9.66HH99 pKa = 6.38KK100 pKa = 10.87NLALKK105 pKa = 8.23TWEE108 pKa = 4.1VALLDD113 pKa = 4.38SIKK116 pKa = 10.96NQDD119 pKa = 3.53DD120 pKa = 3.43RR121 pKa = 11.84HH122 pKa = 5.28ITVVVDD128 pKa = 3.46KK129 pKa = 11.25QGGNGKK135 pKa = 8.2STFSKK140 pKa = 10.67YY141 pKa = 10.81LEE143 pKa = 4.61ANDD146 pKa = 3.93IADD149 pKa = 3.71VCPVVSDD156 pKa = 4.25EE157 pKa = 4.26YY158 pKa = 11.69NDD160 pKa = 3.51YY161 pKa = 10.74TSYY164 pKa = 11.52CMEE167 pKa = 4.54FPKK170 pKa = 10.35KK171 pKa = 10.46AYY173 pKa = 10.69VFDD176 pKa = 4.44LPRR179 pKa = 11.84ATSIKK184 pKa = 10.03RR185 pKa = 11.84RR186 pKa = 11.84TAMWSGIEE194 pKa = 3.93QIKK197 pKa = 10.96NGLLYY202 pKa = 10.24EE203 pKa = 4.18KK204 pKa = 9.97RR205 pKa = 11.84YY206 pKa = 10.07KK207 pKa = 8.6PRR209 pKa = 11.84KK210 pKa = 7.8MWIEE214 pKa = 3.97PPSILVFTNDD224 pKa = 4.07DD225 pKa = 3.95IPWEE229 pKa = 3.97LLSEE233 pKa = 4.46DD234 pKa = 3.0RR235 pKa = 11.84WDD237 pKa = 3.88AYY239 pKa = 10.69RR240 pKa = 11.84LHH242 pKa = 7.87DD243 pKa = 4.02DD244 pKa = 3.52TLYY247 pKa = 11.27VLSKK251 pKa = 10.8PSDD254 pKa = 3.62DD255 pKa = 4.7YY256 pKa = 11.89INGSNATMDD265 pKa = 3.67EE266 pKa = 4.12DD267 pKa = 4.33SRR269 pKa = 11.84LII271 pKa = 3.85

Molecular weight: 31.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

606

271

335

303.0

34.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.941 ± 0.483

1.32 ± 0.133

7.261 ± 0.765

5.611 ± 1.103

2.64 ± 0.266

6.601 ± 0.434

2.475 ± 0.527

6.106 ± 0.104

5.941 ± 1.588

7.426 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.3 ± 0.447

3.795 ± 0.066

6.436 ± 0.792

2.475 ± 0.163

4.62 ± 0.34

7.591 ± 0.361

6.931 ± 1.1

6.766 ± 0.767

1.98 ± 0.606

4.785 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski