Tomato leaf curl Karnataka virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q98744|Q98744_9GEMI Uncharacterized protein C4 OS=Tomato leaf curl Karnataka virus OX=220932 GN=C4 PE=3 SV=1
MM1 pKa = 7.64AAPNRR6 pKa = 11.84FKK8 pKa = 11.34LNAKK12 pKa = 9.95NYY14 pKa = 8.38FLTYY18 pKa = 9.06PKK20 pKa = 10.53CSLTKK25 pKa = 10.67EE26 pKa = 4.01EE27 pKa = 5.06ALSQFLNLQTPTSKK41 pKa = 10.73KK42 pKa = 9.67FIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.49LHH51 pKa = 6.58EE52 pKa = 6.12DD53 pKa = 3.79GTPHH57 pKa = 6.72LHH59 pKa = 6.66VLIQFEE65 pKa = 5.54GKK67 pKa = 9.21FQCKK71 pKa = 8.51NNRR74 pKa = 11.84FFDD77 pKa = 3.77LTSPTRR83 pKa = 11.84SAHH86 pKa = 4.94FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.34SSSDD99 pKa = 2.92VKK101 pKa = 10.47TYY103 pKa = 8.75MEE105 pKa = 4.98KK106 pKa = 10.86DD107 pKa = 3.28GDD109 pKa = 4.1ILDD112 pKa = 4.24HH113 pKa = 7.29GVFQVDD119 pKa = 3.16GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.91AEE136 pKa = 4.61AINAGSKK143 pKa = 9.93IEE145 pKa = 3.96ALNILRR151 pKa = 11.84EE152 pKa = 4.2KK153 pKa = 10.74APKK156 pKa = 10.19DD157 pKa = 3.52YY158 pKa = 11.21VLQFHH163 pKa = 6.73NLNSNLDD170 pKa = 4.14RR171 pKa = 11.84IFTPPMEE178 pKa = 4.56VYY180 pKa = 10.67VSPFSSSSFDD190 pKa = 3.41QVPEE194 pKa = 3.98EE195 pKa = 4.13LEE197 pKa = 3.58EE198 pKa = 3.9WAAEE202 pKa = 4.11NVVNAAARR210 pKa = 11.84PLRR213 pKa = 11.84PVSIVIEE220 pKa = 4.4GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.06DD262 pKa = 4.24VDD264 pKa = 4.0PNYY267 pKa = 10.84LKK269 pKa = 10.68HH270 pKa = 6.42FKK272 pKa = 10.61EE273 pKa = 4.3FMGAQRR279 pKa = 11.84DD280 pKa = 3.71WQSNRR285 pKa = 11.84KK286 pKa = 8.25YY287 pKa = 10.7NKK289 pKa = 8.79PVQIKK294 pKa = 10.46GGIPTIFLCNPGPNSSYY311 pKa = 11.34KK312 pKa = 10.62EE313 pKa = 3.85FLDD316 pKa = 3.72EE317 pKa = 4.61EE318 pKa = 4.71KK319 pKa = 10.9NSKK322 pKa = 9.32LKK324 pKa = 10.68NWAIKK329 pKa = 10.18NATFVTPEE337 pKa = 4.02GPLYY341 pKa = 11.01SGTNQSAAQASQEE354 pKa = 3.86GDD356 pKa = 3.31QTSTSS361 pKa = 3.42

Molecular weight: 40.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q98741|Q98741_9GEMI Replication enhancer OS=Tomato leaf curl Karnataka virus OX=220932 GN=C3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.97VSRR30 pKa = 11.84AVAPIVRR37 pKa = 11.84VTKK40 pKa = 10.71AKK42 pKa = 10.29AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.67VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 7.0RR173 pKa = 11.84DD174 pKa = 3.31RR175 pKa = 11.84YY176 pKa = 8.78QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.67FYY251 pKa = 11.26DD252 pKa = 3.51SVSNN256 pKa = 3.9

Molecular weight: 29.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1100

97

361

183.3

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.727 ± 0.702

2.182 ± 0.463

4.818 ± 0.508

4.909 ± 0.703

4.455 ± 0.395

4.545 ± 0.384

3.909 ± 0.663

4.818 ± 0.792

6.182 ± 0.649

6.727 ± 0.887

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.737

5.636 ± 0.882

6.182 ± 0.585

4.909 ± 0.606

6.727 ± 1.011

8.182 ± 1.095

5.545 ± 0.616

6.818 ± 1.377

1.364 ± 0.17

3.636 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski