Spirosoma oryzae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Spirosoma

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5655 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0TIR4|A0A2T0TIR4_9BACT Putative NBD/HSP70 family sugar kinase OS=Spirosoma oryzae OX=1469603 GN=CLV58_102352 PE=3 SV=1
MM1 pKa = 7.66ANYY4 pKa = 9.81KK5 pKa = 10.53NEE7 pKa = 3.92GFDD10 pKa = 3.7PEE12 pKa = 4.97EE13 pKa = 4.89IDD15 pKa = 4.45ALKK18 pKa = 10.58EE19 pKa = 3.63EE20 pKa = 4.63CQAAGKK26 pKa = 10.38SFVYY30 pKa = 10.76VDD32 pKa = 5.36DD33 pKa = 4.77NDD35 pKa = 5.02LDD37 pKa = 3.85VLEE40 pKa = 5.23SGEE43 pKa = 4.32CVHH46 pKa = 6.57IQFPGQYY53 pKa = 9.14QGQEE57 pKa = 4.13VIYY60 pKa = 10.27DD61 pKa = 3.55ALVYY65 pKa = 8.55TLRR68 pKa = 11.84LHH70 pKa = 6.7HH71 pKa = 6.86SSLVYY76 pKa = 10.24EE77 pKa = 4.3MAVEE81 pKa = 4.76EE82 pKa = 4.34VQKK85 pKa = 9.69TYY87 pKa = 10.88PDD89 pKa = 3.47YY90 pKa = 10.61TPPEE94 pKa = 4.05EE95 pKa = 4.93RR96 pKa = 11.84EE97 pKa = 4.06AGYY100 pKa = 10.4KK101 pKa = 9.36IAPDD105 pKa = 3.52RR106 pKa = 11.84EE107 pKa = 4.36EE108 pKa = 4.01EE109 pKa = 4.34AEE111 pKa = 4.12TALTEE116 pKa = 4.32IIGEE120 pKa = 4.16IEE122 pKa = 3.9DD123 pKa = 4.09TEE125 pKa = 4.55TVKK128 pKa = 10.73VQEE131 pKa = 4.93HH132 pKa = 6.44IEE134 pKa = 3.95IDD136 pKa = 3.36TDD138 pKa = 3.23SDD140 pKa = 3.98YY141 pKa = 11.8GVALDD146 pKa = 3.71VCLNTEE152 pKa = 4.74DD153 pKa = 4.4ISDD156 pKa = 3.68EE157 pKa = 4.19VIEE160 pKa = 5.28NFIQQFKK167 pKa = 11.14ANTLALDD174 pKa = 3.47TTLYY178 pKa = 11.01SFTSEE183 pKa = 4.49DD184 pKa = 3.88DD185 pKa = 3.51EE186 pKa = 4.7

Molecular weight: 21.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0RUG1|A0A2T0RUG1_9BACT Zn_protease domain-containing protein OS=Spirosoma oryzae OX=1469603 GN=CLV58_13812 PE=4 SV=1
MM1 pKa = 7.66GEE3 pKa = 4.05LHH5 pKa = 6.03QRR7 pKa = 11.84PSPFRR12 pKa = 11.84IRR14 pKa = 11.84LRR16 pKa = 11.84WVYY19 pKa = 10.2CVVWYY24 pKa = 10.37RR25 pKa = 11.84INCLHH30 pKa = 6.89RR31 pKa = 11.84LSLTDD36 pKa = 3.16PAGSVRR42 pKa = 11.84VGRR45 pKa = 11.84SRR47 pKa = 11.84DD48 pKa = 3.46GQAVVAGPTQGLCPTACSVQKK69 pKa = 10.13STDD72 pKa = 3.39HH73 pKa = 6.75PPEE76 pKa = 4.38LSCLIPCAQWSDD88 pKa = 3.4RR89 pKa = 11.84TVNSQDD95 pKa = 3.73SACLNAVQTKK105 pKa = 7.0CHH107 pKa = 6.72CEE109 pKa = 3.39PGFISRR115 pKa = 11.84QRR117 pKa = 11.84KK118 pKa = 6.04AQRR121 pKa = 11.84IVRR124 pKa = 11.84TVRR127 pKa = 11.84LSAEE131 pKa = 4.12GAAGSIKK138 pKa = 9.71TGRR141 pKa = 11.84PVYY144 pKa = 10.3CNCTTKK150 pKa = 10.73CVGTCLITDD159 pKa = 4.25TASGEE164 pKa = 4.13PIRR167 pKa = 11.84TKK169 pKa = 10.63RR170 pKa = 11.84QLTRR174 pKa = 11.84IDD176 pKa = 3.57RR177 pKa = 11.84AVLGSSSILTVATTIRR193 pKa = 11.84SRR195 pKa = 11.84PRR197 pKa = 11.84SAIVLRR203 pKa = 11.84TTEE206 pKa = 3.93SWVQRR211 pKa = 11.84YY212 pKa = 6.65WVPRR216 pKa = 11.84SRR218 pKa = 4.43

Molecular weight: 24.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5655

0

5655

1917797

24

3356

339.1

37.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.703 ± 0.036

0.738 ± 0.011

5.578 ± 0.025

4.698 ± 0.034

4.145 ± 0.022

7.303 ± 0.04

1.78 ± 0.017

5.455 ± 0.029

4.373 ± 0.038

10.163 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.017

4.47 ± 0.032

4.811 ± 0.024

4.669 ± 0.028

5.716 ± 0.032

6.209 ± 0.035

6.908 ± 0.049

7.055 ± 0.029

1.295 ± 0.012

3.825 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski