Desulfosporosinus sp. Sb-LF

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfosporosinus; unclassified Desulfosporosinus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3743 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0QRB1|A0A4Z0QRB1_9FIRM Bacillithiol biosynthesis deacetylase BshB2 OS=Desulfosporosinus sp. Sb-LF OX=2560027 GN=bshB2 PE=4 SV=1
MM1 pKa = 7.28IAAVDD6 pKa = 3.59KK7 pKa = 8.54DD8 pKa = 3.75TCIGCGACPEE18 pKa = 4.36FCPEE22 pKa = 3.95VFKK25 pKa = 11.29MEE27 pKa = 4.85DD28 pKa = 3.24DD29 pKa = 4.18GLAVAYY35 pKa = 7.35TNPIPSDD42 pKa = 3.46VEE44 pKa = 4.0AAAKK48 pKa = 10.08DD49 pKa = 3.65AADD52 pKa = 4.28GCPVDD57 pKa = 5.44AIHH60 pKa = 7.29VNGG63 pKa = 4.12

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0QUI4|A0A4Z0QUI4_9FIRM SdpI family protein OS=Desulfosporosinus sp. Sb-LF OX=2560027 GN=E4K68_05520 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 9.19QPKK8 pKa = 8.78NRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.44KK13 pKa = 10.09RR14 pKa = 11.84VHH16 pKa = 5.93GFLSRR21 pKa = 11.84MSTPTGRR28 pKa = 11.84NVLKK32 pKa = 10.51RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.57GRR39 pKa = 11.84KK40 pKa = 8.8KK41 pKa = 10.81LSVV44 pKa = 3.15

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3743

0

3743

1146958

17

2864

306.4

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.638 ± 0.047

1.094 ± 0.018

4.865 ± 0.03

6.678 ± 0.054

4.038 ± 0.031

7.57 ± 0.044

1.921 ± 0.017

7.373 ± 0.04

5.949 ± 0.038

10.413 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.688 ± 0.017

4.095 ± 0.026

3.933 ± 0.026

3.796 ± 0.029

4.737 ± 0.038

6.145 ± 0.034

5.524 ± 0.045

7.372 ± 0.034

1.077 ± 0.017

3.092 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski