Sweet potato leaf curl Spain virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6LI28|I6LI28_9GEMI Replication-associated protein OS=Sweet potato leaf curl Spain virus OX=652717 GN=C1 PE=3 SV=1
MM1 pKa = 7.31PRR3 pKa = 11.84AGRR6 pKa = 11.84FNINAKK12 pKa = 10.36NYY14 pKa = 9.14FLTYY18 pKa = 7.87PQCSISKK25 pKa = 10.05EE26 pKa = 3.79EE27 pKa = 4.29ALAQILNIPTAVNKK41 pKa = 10.32KK42 pKa = 9.63FIKK45 pKa = 9.69ICRR48 pKa = 11.84EE49 pKa = 3.76LHH51 pKa = 6.97EE52 pKa = 5.88DD53 pKa = 3.95GQPHH57 pKa = 6.66LHH59 pKa = 6.38VLLQFEE65 pKa = 5.61GKK67 pKa = 9.18FQCTNQRR74 pKa = 11.84LFDD77 pKa = 4.26LVSQTRR83 pKa = 11.84SAHH86 pKa = 4.74FHH88 pKa = 6.49PNIQRR93 pKa = 11.84AKK95 pKa = 9.77SSSDD99 pKa = 2.99VKK101 pKa = 11.22SYY103 pKa = 11.11VDD105 pKa = 3.65KK106 pKa = 11.62DD107 pKa = 3.31GDD109 pKa = 3.77TLEE112 pKa = 4.1WGEE115 pKa = 3.95FQVDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSKK143 pKa = 10.24DD144 pKa = 3.18AALQIIRR151 pKa = 11.84EE152 pKa = 4.06KK153 pKa = 10.91LPEE156 pKa = 3.95KK157 pKa = 10.64FIFQYY162 pKa = 11.08HH163 pKa = 5.73NLVSNLDD170 pKa = 4.36RR171 pKa = 11.84IFSPPPSVYY180 pKa = 10.41SSPFSISSFNNVPDD194 pKa = 5.62IISDD198 pKa = 3.84WAAEE202 pKa = 4.12NVMDD206 pKa = 5.32AAARR210 pKa = 11.84PEE212 pKa = 4.01RR213 pKa = 11.84PISIVIEE220 pKa = 4.71GPSRR224 pKa = 11.84MGKK227 pKa = 6.66TVWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.09VYY251 pKa = 10.85SNSAWYY257 pKa = 10.34NVIDD261 pKa = 4.95DD262 pKa = 4.8VNPQYY267 pKa = 11.15LKK269 pKa = 10.65HH270 pKa = 6.26FKK272 pKa = 10.69EE273 pKa = 4.43FMGAQKK279 pKa = 10.56DD280 pKa = 3.62WQSNCKK286 pKa = 9.38YY287 pKa = 10.21GKK289 pKa = 9.18PVQIKK294 pKa = 10.41GGIPTIFLCNPGEE307 pKa = 4.28GSSFKK312 pKa = 10.83LWLDD316 pKa = 3.35KK317 pKa = 11.08PEE319 pKa = 4.24QEE321 pKa = 5.02ALKK324 pKa = 10.68NWAVKK329 pKa = 10.42NAVFCDD335 pKa = 3.19VDD337 pKa = 4.13SPFWIQEE344 pKa = 4.27EE345 pKa = 4.8VSHH348 pKa = 6.74SGTNTRR354 pKa = 11.84GGQEE358 pKa = 3.91EE359 pKa = 4.49PEE361 pKa = 4.34EE362 pKa = 4.15NSS364 pKa = 3.18

Molecular weight: 40.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6LI26|I6LI26_9GEMI Replication enhancer OS=Sweet potato leaf curl Spain virus OX=652717 GN=C3 PE=3 SV=1
MM1 pKa = 7.12TGRR4 pKa = 11.84MRR6 pKa = 11.84VTRR9 pKa = 11.84RR10 pKa = 11.84FHH12 pKa = 7.1PYY14 pKa = 9.34GGTPARR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84LNFEE26 pKa = 3.95TAIVPYY32 pKa = 9.22TGNAVPIAARR42 pKa = 11.84SYY44 pKa = 10.33VPISRR49 pKa = 11.84SAQMKK54 pKa = 9.26RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84GDD59 pKa = 4.08RR60 pKa = 11.84IPKK63 pKa = 9.96GCVGPCKK70 pKa = 10.08VQDD73 pKa = 3.86YY74 pKa = 8.72EE75 pKa = 4.55FKK77 pKa = 10.32MDD79 pKa = 3.61VPHH82 pKa = 7.03SGTFVCVSDD91 pKa = 3.96FTRR94 pKa = 11.84GTGLTHH100 pKa = 7.28RR101 pKa = 11.84LGKK104 pKa = 9.86RR105 pKa = 11.84VCIKK109 pKa = 11.05SMGIDD114 pKa = 3.27GKK116 pKa = 11.25VWMDD120 pKa = 3.8DD121 pKa = 3.32NVAKK125 pKa = 10.49GDD127 pKa = 3.52HH128 pKa = 6.23TNIITYY134 pKa = 9.02WLIRR138 pKa = 11.84DD139 pKa = 3.65RR140 pKa = 11.84RR141 pKa = 11.84PNKK144 pKa = 10.44DD145 pKa = 3.19PLTFSQAFTMYY156 pKa = 10.83DD157 pKa = 3.65NEE159 pKa = 4.15PTTAKK164 pKa = 10.17IRR166 pKa = 11.84MDD168 pKa = 3.48LRR170 pKa = 11.84DD171 pKa = 3.42RR172 pKa = 11.84MQVLKK177 pKa = 10.63KK178 pKa = 10.18FAVTVSGGPYY188 pKa = 8.51NHH190 pKa = 7.45KK191 pKa = 10.06EE192 pKa = 3.61QALVRR197 pKa = 11.84KK198 pKa = 8.56FFNSLYY204 pKa = 10.63NHH206 pKa = 5.01VTYY209 pKa = 10.84NHH211 pKa = 6.65KK212 pKa = 10.86EE213 pKa = 3.59EE214 pKa = 4.51AKK216 pKa = 10.79YY217 pKa = 10.68EE218 pKa = 4.05NHH220 pKa = 7.03LEE222 pKa = 4.07NALMLYY228 pKa = 9.88SASSHH233 pKa = 6.23ASNPVYY239 pKa = 9.46QTLRR243 pKa = 11.84CRR245 pKa = 11.84AYY247 pKa = 10.5FYY249 pKa = 10.73DD250 pKa = 3.47SHH252 pKa = 8.3KK253 pKa = 11.26NN254 pKa = 3.32

Molecular weight: 29.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1110

85

364

185.0

21.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.126 ± 0.513

2.973 ± 0.712

4.955 ± 0.403

5.405 ± 0.845

4.054 ± 0.548

5.495 ± 0.336

3.333 ± 0.369

5.586 ± 0.62

6.126 ± 0.344

7.117 ± 1.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.072 ± 0.574

5.586 ± 0.602

6.036 ± 0.681

4.414 ± 0.771

6.486 ± 1.176

8.198 ± 0.967

5.586 ± 1.192

4.865 ± 0.812

1.982 ± 0.319

3.604 ± 0.615
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski