Ruminococcus sp. CAG:330

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7F8N2|R7F8N2_9FIRM Uncharacterized protein OS=Ruminococcus sp. CAG:330 OX=1262954 GN=BN611_00979 PE=4 SV=1
MM1 pKa = 7.38NKK3 pKa = 9.98LKK5 pKa = 9.61KK6 pKa = 8.94TLALMAALTMTATAFVGCGDD26 pKa = 4.54KK27 pKa = 10.83EE28 pKa = 4.46DD29 pKa = 4.23SSSTADD35 pKa = 3.23SSVAEE40 pKa = 4.27SSADD44 pKa = 3.48EE45 pKa = 4.28SSADD49 pKa = 3.57AEE51 pKa = 4.37SSEE54 pKa = 4.97DD55 pKa = 3.67ADD57 pKa = 3.58SSEE60 pKa = 5.11AEE62 pKa = 4.39GGDD65 pKa = 3.47TTGAPTPDD73 pKa = 3.48GTLKK77 pKa = 11.03DD78 pKa = 4.55DD79 pKa = 5.79DD80 pKa = 4.31DD81 pKa = 4.47TLSILCWTDD90 pKa = 2.66TDD92 pKa = 4.1LKK94 pKa = 11.65AMFDD98 pKa = 3.56VTSAKK103 pKa = 10.46NPTYY107 pKa = 11.19VNVGSGGTEE116 pKa = 3.5ANEE119 pKa = 3.9QYY121 pKa = 10.82VQYY124 pKa = 9.9FSSGSDD130 pKa = 2.79VDD132 pKa = 5.81LFVCDD137 pKa = 4.45ADD139 pKa = 3.12WVMSYY144 pKa = 11.02EE145 pKa = 4.25NNDD148 pKa = 3.45EE149 pKa = 4.11YY150 pKa = 10.98TAPLSALGIDD160 pKa = 3.01EE161 pKa = 5.48SMYY164 pKa = 10.98KK165 pKa = 10.08DD166 pKa = 3.14AYY168 pKa = 10.37GYY170 pKa = 8.56TVTLGTDD177 pKa = 3.15SNGVLKK183 pKa = 10.58GASWQAAAGGYY194 pKa = 10.28AYY196 pKa = 10.52RR197 pKa = 11.84ADD199 pKa = 3.8LAEE202 pKa = 4.73EE203 pKa = 4.22YY204 pKa = 10.85LGVTSPEE211 pKa = 3.94DD212 pKa = 3.38MQAQIGDD219 pKa = 3.37WDD221 pKa = 4.47AFWKK225 pKa = 8.78TAATVYY231 pKa = 10.03EE232 pKa = 4.02KK233 pKa = 10.9SGNKK237 pKa = 8.06TAMADD242 pKa = 3.97TIAGVWRR249 pKa = 11.84AYY251 pKa = 10.45SAGNRR256 pKa = 11.84TTPWIDD262 pKa = 3.31ADD264 pKa = 5.0GKK266 pKa = 9.77FQPDD270 pKa = 3.13ATKK273 pKa = 11.09DD274 pKa = 3.87FITMAKK280 pKa = 10.04EE281 pKa = 3.99NFDD284 pKa = 3.03KK285 pKa = 11.43GYY287 pKa = 8.17ITNVKK292 pKa = 9.08QWTDD296 pKa = 2.74DD297 pKa = 3.08WKK299 pKa = 11.43LIGQSEE305 pKa = 4.42GALANATFGYY315 pKa = 9.03FFPSWSMAAGAQLQDD330 pKa = 3.63GEE332 pKa = 4.48GGEE335 pKa = 4.56GGSTYY340 pKa = 10.91GKK342 pKa = 9.98YY343 pKa = 10.53GYY345 pKa = 9.48TMGPTGWYY353 pKa = 8.51WGGSWLCVSPNCNSATAAAQFVYY376 pKa = 10.98DD377 pKa = 3.59MTINADD383 pKa = 3.4TMKK386 pKa = 10.49QYY388 pKa = 11.58ALSHH392 pKa = 6.39SDD394 pKa = 3.25FVNNKK399 pKa = 7.5TVMADD404 pKa = 3.45VVAEE408 pKa = 4.28GANKK412 pKa = 10.17NPLLKK417 pKa = 10.76DD418 pKa = 3.54GQDD421 pKa = 3.33QFATLLDD428 pKa = 3.89SADD431 pKa = 4.18NISLDD436 pKa = 3.64GVAGQNDD443 pKa = 4.16GTINDD448 pKa = 3.91AFVTAVQSYY457 pKa = 7.8CTGDD461 pKa = 3.56LDD463 pKa = 5.35SEE465 pKa = 4.54EE466 pKa = 4.79ACLDD470 pKa = 3.57NFLDD474 pKa = 4.23AVSTALPDD482 pKa = 3.7VQVDD486 pKa = 3.29

Molecular weight: 51.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7FBU5|R7FBU5_9FIRM Uncharacterized protein conserved in bacteria (DUF2325) OS=Ruminococcus sp. CAG:330 OX=1262954 GN=BN611_01232 PE=3 SV=1
MM1 pKa = 7.29EE2 pKa = 6.02LEE4 pKa = 4.21TFFTVPEE11 pKa = 3.97QTRR14 pKa = 11.84LLFCAVFLGIPLGLCFDD31 pKa = 4.06VLRR34 pKa = 11.84VLRR37 pKa = 11.84MLLPHH42 pKa = 6.8GKK44 pKa = 9.58LAVALEE50 pKa = 4.23DD51 pKa = 3.65TAFLLVWGGALLCFAGIFARR71 pKa = 11.84GEE73 pKa = 3.65MRR75 pKa = 11.84AYY77 pKa = 9.89YY78 pKa = 10.75ALGSTLGFLLYY89 pKa = 10.14RR90 pKa = 11.84CTIGTVTVRR99 pKa = 11.84VLHH102 pKa = 6.73RR103 pKa = 11.84IFGVSGRR110 pKa = 11.84VLRR113 pKa = 11.84WLTTPLFHH121 pKa = 6.75GVVRR125 pKa = 11.84IYY127 pKa = 11.19GVLKK131 pKa = 10.47EE132 pKa = 4.4KK133 pKa = 10.44FGHH136 pKa = 5.46FAKK139 pKa = 10.77VSGKK143 pKa = 7.59VHH145 pKa = 6.41FFWHH149 pKa = 6.52LPLIVIRR156 pKa = 11.84KK157 pKa = 8.57VLYY160 pKa = 8.42NNKK163 pKa = 9.58RR164 pKa = 11.84KK165 pKa = 7.31TVKK168 pKa = 10.28RR169 pKa = 3.71

Molecular weight: 19.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2234

0

2234

701908

29

2801

314.2

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.947 ± 0.062

1.886 ± 0.027

5.644 ± 0.046

6.769 ± 0.053

3.906 ± 0.035

7.124 ± 0.053

2.013 ± 0.026

6.173 ± 0.05

5.299 ± 0.057

9.203 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.026

3.737 ± 0.037

3.759 ± 0.033

4.043 ± 0.046

4.792 ± 0.05

6.037 ± 0.049

6.161 ± 0.086

6.832 ± 0.039

0.981 ± 0.019

3.839 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski