Marinibacterium profundimaris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Marinibacterium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5086 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A225NMM7|A0A225NMM7_9RHOB Uncharacterized protein OS=Marinibacterium profundimaris OX=1679460 GN=ATO3_16680 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 8.99KK3 pKa = 8.29TLFTSTALVLLAGAAAAQVSFSGYY27 pKa = 9.81GRR29 pKa = 11.84FGLGYY34 pKa = 10.13QEE36 pKa = 5.28DD37 pKa = 4.14RR38 pKa = 11.84QITDD42 pKa = 2.66INGDD46 pKa = 3.52VTDD49 pKa = 4.11TDD51 pKa = 3.51VSLVSRR57 pKa = 11.84FRR59 pKa = 11.84LNIDD63 pKa = 3.4GMAVTDD69 pKa = 3.86GGVEE73 pKa = 4.25FSARR77 pKa = 11.84VRR79 pKa = 11.84LQADD83 pKa = 3.59DD84 pKa = 4.46SPAFNEE90 pKa = 4.06QNSASLNGARR100 pKa = 11.84YY101 pKa = 8.52SVQYY105 pKa = 10.92GGFRR109 pKa = 11.84VDD111 pKa = 3.43AGNVAGSFDD120 pKa = 4.34NMPNYY125 pKa = 10.68YY126 pKa = 10.27GFEE129 pKa = 4.59PGLEE133 pKa = 4.09AFTGQYY139 pKa = 9.93TGVDD143 pKa = 3.91YY144 pKa = 11.45DD145 pKa = 3.88FLAYY149 pKa = 10.48SSTGSGSNAVYY160 pKa = 10.55ASYY163 pKa = 9.07TAGGFFFGASYY174 pKa = 10.6DD175 pKa = 3.71QEE177 pKa = 5.18AFLDD181 pKa = 3.6NDD183 pKa = 3.13RR184 pKa = 11.84WDD186 pKa = 3.19IGGSYY191 pKa = 9.1TFNNFTAALTYY202 pKa = 10.64GEE204 pKa = 4.54NEE206 pKa = 3.69IDD208 pKa = 3.39QSLLVFTLGADD219 pKa = 4.02FDD221 pKa = 4.58RR222 pKa = 11.84FSGTLFVGDD231 pKa = 4.55EE232 pKa = 4.51DD233 pKa = 5.44LDD235 pKa = 4.27LAAGAPDD242 pKa = 3.29IDD244 pKa = 3.65GTVYY248 pKa = 10.88GLSLSFDD255 pKa = 3.33VGAATSILASYY266 pKa = 7.92GTGTGDD272 pKa = 5.68DD273 pKa = 3.96DD274 pKa = 5.0DD275 pKa = 4.79EE276 pKa = 6.95GYY278 pKa = 11.29GVAFVHH284 pKa = 6.95DD285 pKa = 4.62LGGGVSLKK293 pKa = 10.91GGIGAEE299 pKa = 4.38GPKK302 pKa = 10.11DD303 pKa = 3.6DD304 pKa = 4.9SYY306 pKa = 11.67MVGDD310 pKa = 4.74LGVLFNFF317 pKa = 4.97

Molecular weight: 33.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A225NQN9|A0A225NQN9_9RHOB Riboflavin synthase subunit alpha OS=Marinibacterium profundimaris OX=1679460 GN=ATO3_10995 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5086

0

5086

1621870

31

3016

318.9

34.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.406 ± 0.051

0.879 ± 0.011

6.2 ± 0.031

6.018 ± 0.032

3.652 ± 0.023

8.945 ± 0.045

2.018 ± 0.017

5.038 ± 0.029

2.711 ± 0.027

10.096 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.015

2.352 ± 0.019

5.336 ± 0.029

3.049 ± 0.018

6.947 ± 0.037

5.03 ± 0.029

5.524 ± 0.024

7.318 ± 0.024

1.429 ± 0.016

2.228 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski