Holzapfelia floricola DSM 23037 = JCM 16512

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Holzapfelia; Holzapfelia floricola

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1222 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R2DK45|A0A0R2DK45_9LACO AlbA_2 domain-containing protein OS=Holzapfelia floricola DSM 23037 = JCM 16512 OX=1423744 GN=FC86_GL000584 PE=4 SV=1
MM1 pKa = 7.23NLSIPSYY8 pKa = 10.77FDD10 pKa = 3.2YY11 pKa = 10.85KK12 pKa = 11.02KK13 pKa = 11.02EE14 pKa = 4.11DD15 pKa = 3.57FTLTTAKK22 pKa = 9.67IVYY25 pKa = 10.68ASMTGNNEE33 pKa = 3.75EE34 pKa = 4.03VASIIDD40 pKa = 3.85EE41 pKa = 4.3KK42 pKa = 10.61LQDD45 pKa = 3.62MGIQTDD51 pKa = 3.43MSDD54 pKa = 2.81ITFSDD59 pKa = 3.55VNEE62 pKa = 3.86FSEE65 pKa = 4.28YY66 pKa = 10.69DD67 pKa = 3.19IAFMVPYY74 pKa = 10.13SYY76 pKa = 11.8GEE78 pKa = 4.23GEE80 pKa = 4.75LPDD83 pKa = 3.87EE84 pKa = 4.77ALDD87 pKa = 4.07FYY89 pKa = 11.61EE90 pKa = 5.78DD91 pKa = 3.97LLEE94 pKa = 6.05IDD96 pKa = 3.94LTDD99 pKa = 5.0KK100 pKa = 10.89IFAVAGSGDD109 pKa = 3.71VFYY112 pKa = 11.13EE113 pKa = 3.84DD114 pKa = 5.13AYY116 pKa = 11.11CITVDD121 pKa = 4.64DD122 pKa = 4.08FTKK125 pKa = 10.94AFLKK129 pKa = 10.62IGGIQAAEE137 pKa = 3.89SLKK140 pKa = 10.85INLAPDD146 pKa = 3.55LEE148 pKa = 5.93DD149 pKa = 4.08IEE151 pKa = 5.6NIEE154 pKa = 4.1QFTEE158 pKa = 4.05KK159 pKa = 10.62AVNFYY164 pKa = 9.93EE165 pKa = 4.5ANHH168 pKa = 6.32KK169 pKa = 10.08

Molecular weight: 19.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R2DTX9|A0A0R2DTX9_9LACO Phosphoribosylglycinamide formyltransferase OS=Holzapfelia floricola DSM 23037 = JCM 16512 OX=1423744 GN=purN PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.12QKK5 pKa = 7.54THH7 pKa = 6.73RR8 pKa = 11.84GSAKK12 pKa = 10.13RR13 pKa = 11.84FKK15 pKa = 9.74KK16 pKa = 8.27TANGGLKK23 pKa = 9.49RR24 pKa = 11.84HH25 pKa = 6.15HH26 pKa = 7.23AFTGHH31 pKa = 6.77RR32 pKa = 11.84FHH34 pKa = 7.54GKK36 pKa = 6.24TKK38 pKa = 9.47KK39 pKa = 9.37QRR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.7LRR45 pKa = 11.84KK46 pKa = 9.35SAMVSASDD54 pKa = 3.79FKK56 pKa = 10.9RR57 pKa = 11.84IKK59 pKa = 10.67QMLSQVRR66 pKa = 3.52

Molecular weight: 7.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1222

0

1222

383689

45

1884

314.0

35.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.679 ± 0.067

0.374 ± 0.014

5.782 ± 0.061

6.114 ± 0.069

4.449 ± 0.058

6.049 ± 0.064

1.883 ± 0.033

7.765 ± 0.065

7.224 ± 0.058

9.886 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 0.034

5.63 ± 0.082

3.259 ± 0.034

5.044 ± 0.067

3.556 ± 0.053

6.662 ± 0.063

5.864 ± 0.046

6.781 ± 0.061

0.732 ± 0.021

3.743 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski