TM7 phylum sp. oral taxon 356

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Saccharibacteria

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 611 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A563AES4|A0A563AES4_9BACT Phage holin family protein OS=TM7 phylum sp. oral taxon 356 OX=713057 GN=EUA81_01605 PE=4 SV=1
MM1 pKa = 7.92HH2 pKa = 6.91YY3 pKa = 10.49NQYY6 pKa = 10.2QRR8 pKa = 11.84LINIVGGLYY17 pKa = 10.22EE18 pKa = 3.93NHH20 pKa = 6.83LGYY23 pKa = 10.4FDD25 pKa = 5.94DD26 pKa = 4.14LTAEE30 pKa = 4.17EE31 pKa = 4.44RR32 pKa = 11.84QVLSRR37 pKa = 11.84VFFYY41 pKa = 10.56DD42 pKa = 3.2YY43 pKa = 10.81DD44 pKa = 4.39YY45 pKa = 11.67DD46 pKa = 5.76SEE48 pKa = 4.9DD49 pKa = 4.32CPDD52 pKa = 4.66DD53 pKa = 4.18FPEE56 pKa = 4.26SFPDD60 pKa = 4.05FFRR63 pKa = 11.84DD64 pKa = 4.54RR65 pKa = 11.84IAGNQALQDD74 pKa = 3.58EE75 pKa = 4.86ALAAVARR82 pKa = 11.84LYY84 pKa = 11.45AMSGMGDD91 pKa = 3.74FALTRR96 pKa = 11.84VSDD99 pKa = 3.68KK100 pKa = 11.03PLL102 pKa = 4.58

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A563ADB0|A0A563ADB0_9BACT Response regulator OS=TM7 phylum sp. oral taxon 356 OX=713057 GN=EUA81_02960 PE=4 SV=1
MM1 pKa = 7.04YY2 pKa = 10.13KK3 pKa = 10.19VNRR6 pKa = 11.84AMEE9 pKa = 4.3RR10 pKa = 11.84WRR12 pKa = 11.84YY13 pKa = 7.49NANRR17 pKa = 11.84VSAGGSSWAGRR28 pKa = 11.84VDD30 pKa = 3.65PAKK33 pKa = 10.59SRR35 pKa = 11.84QNQITARR42 pKa = 11.84QIKK45 pKa = 10.57ADD47 pKa = 3.75LFDD50 pKa = 6.13DD51 pKa = 4.18NGEE54 pKa = 3.97LKK56 pKa = 10.48LARR59 pKa = 11.84RR60 pKa = 11.84HH61 pKa = 5.71RR62 pKa = 11.84ADD64 pKa = 3.15HH65 pKa = 6.62GIVALAVALSIFGVVIIYY83 pKa = 10.27SIASGLYY90 pKa = 9.25GGNTTVINQEE100 pKa = 3.79MLKK103 pKa = 10.41RR104 pKa = 11.84GIFLAVGVVLFIVASRR120 pKa = 11.84IPLNWWKK127 pKa = 10.7KK128 pKa = 7.63AGPYY132 pKa = 9.89IFLIALLICLALPILGAAHH151 pKa = 6.15VPVARR156 pKa = 11.84CALGACRR163 pKa = 11.84WYY165 pKa = 10.88KK166 pKa = 10.69LGVVSFQPAEE176 pKa = 3.93FLKK179 pKa = 10.99LGTVLFIAGYY189 pKa = 10.4LSTRR193 pKa = 11.84LANGKK198 pKa = 10.06LNDD201 pKa = 4.04KK202 pKa = 9.34LTLAEE207 pKa = 4.22VLAVMLVSLLIIVGLQRR224 pKa = 11.84DD225 pKa = 4.21MGTGAAIVAIFLIEE239 pKa = 3.93LVMSGMSWRR248 pKa = 11.84KK249 pKa = 8.75LAIVLGVIVLLFVVMTLAAPHH270 pKa = 5.86RR271 pKa = 11.84MSRR274 pKa = 11.84IFTFTKK280 pKa = 10.14SGSNADD286 pKa = 3.07SDD288 pKa = 4.08YY289 pKa = 11.06HH290 pKa = 8.11INQALIGLGSGGLTGRR306 pKa = 11.84GLGQSVQAFGWLPEE320 pKa = 4.11AVNDD324 pKa = 4.5SIFAIVGEE332 pKa = 4.24TLGFVGTVGLIATFAFLIMRR352 pKa = 11.84MFSKK356 pKa = 9.91TNYY359 pKa = 8.95LNNMYY364 pKa = 10.36LRR366 pKa = 11.84LVVAGVVGWLASHH379 pKa = 6.61VLANVMAMTHH389 pKa = 7.11LIPLTGITLPLVSSGGTSAIFVMISLGVVFEE420 pKa = 4.19ISCYY424 pKa = 7.0TAHH427 pKa = 6.9RR428 pKa = 11.84RR429 pKa = 11.84IDD431 pKa = 3.2IAEE434 pKa = 4.13VNEE437 pKa = 4.31SQGGDD442 pKa = 3.27SEE444 pKa = 4.84NPVRR448 pKa = 11.84RR449 pKa = 11.84RR450 pKa = 11.84RR451 pKa = 11.84QRR453 pKa = 11.84WSHH456 pKa = 4.51STDD459 pKa = 2.61RR460 pKa = 11.84RR461 pKa = 11.84RR462 pKa = 11.84SRR464 pKa = 11.84TTTSAGG470 pKa = 3.27

Molecular weight: 51.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

611

0

611

181644

25

1106

297.3

33.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.442 ± 0.104

0.747 ± 0.026

6.175 ± 0.094

5.518 ± 0.096

3.47 ± 0.064

6.732 ± 0.092

1.915 ± 0.048

7.115 ± 0.099

6.032 ± 0.087

9.328 ± 0.112

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.041

4.421 ± 0.075

3.994 ± 0.075

4.167 ± 0.077

5.757 ± 0.075

6.829 ± 0.089

5.576 ± 0.074

6.84 ± 0.09

1.056 ± 0.032

3.577 ± 0.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski