Amino acid dipepetide frequency for Synechococcus sp. (strain ATCC 27167 / PCC 6312)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.834AlaAla: 8.834 ± 0.122
0.989AlaCys: 0.989 ± 0.034
4.226AlaAsp: 4.226 ± 0.08
5.413AlaGlu: 5.413 ± 0.085
2.752AlaPhe: 2.752 ± 0.056
6.401AlaGly: 6.401 ± 0.094
1.518AlaHis: 1.518 ± 0.041
5.92AlaIle: 5.92 ± 0.09
3.475AlaLys: 3.475 ± 0.065
9.813AlaLeu: 9.813 ± 0.108
1.908AlaMet: 1.908 ± 0.054
3.236AlaAsn: 3.236 ± 0.078
3.955AlaPro: 3.955 ± 0.079
5.562AlaGln: 5.562 ± 0.09
4.106AlaArg: 4.106 ± 0.061
4.562AlaSer: 4.562 ± 0.072
5.237AlaThr: 5.237 ± 0.079
5.882AlaVal: 5.882 ± 0.074
2.388AlaTrp: 2.388 ± 0.061
2.525AlaTyr: 2.525 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.551CysAla: 0.551 ± 0.025
0.16CysCys: 0.16 ± 0.013
0.52CysAsp: 0.52 ± 0.022
0.457CysGlu: 0.457 ± 0.021
0.397CysPhe: 0.397 ± 0.02
0.78CysGly: 0.78 ± 0.031
0.319CysHis: 0.319 ± 0.018
0.459CysIle: 0.459 ± 0.021
0.251CysLys: 0.251 ± 0.015
1.284CysLeu: 1.284 ± 0.035
0.147CysMet: 0.147 ± 0.013
0.286CysAsn: 0.286 ± 0.015
0.673CysPro: 0.673 ± 0.025
0.781CysGln: 0.781 ± 0.028
0.568CysArg: 0.568 ± 0.026
0.534CysSer: 0.534 ± 0.023
0.412CysThr: 0.412 ± 0.02
0.531CysVal: 0.531 ± 0.027
0.163CysTrp: 0.163 ± 0.012
0.375CysTyr: 0.375 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
3.538AspAla: 3.538 ± 0.061
0.574AspCys: 0.574 ± 0.025
2.046AspAsp: 2.046 ± 0.059
2.465AspGlu: 2.465 ± 0.059
2.258AspPhe: 2.258 ± 0.048
3.132AspGly: 3.132 ± 0.075
1.053AspHis: 1.053 ± 0.032
3.063AspIle: 3.063 ± 0.058
1.465AspLys: 1.465 ± 0.045
6.435AspLeu: 6.435 ± 0.083
0.75AspMet: 0.75 ± 0.029
1.465AspAsn: 1.465 ± 0.039
2.93AspPro: 2.93 ± 0.068
2.83AspGln: 2.83 ± 0.052
2.839AspArg: 2.839 ± 0.06
2.625AspSer: 2.625 ± 0.052
2.223AspThr: 2.223 ± 0.053
3.226AspVal: 3.226 ± 0.056
0.996AspTrp: 0.996 ± 0.03
1.927AspTyr: 1.927 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
5.035GluAla: 5.035 ± 0.084
0.389GluCys: 0.389 ± 0.019
2.41GluAsp: 2.41 ± 0.058
2.94GluGlu: 2.94 ± 0.067
2.452GluPhe: 2.452 ± 0.046
2.782GluGly: 2.782 ± 0.052
0.976GluHis: 0.976 ± 0.034
4.342GluIle: 4.342 ± 0.069
2.562GluLys: 2.562 ± 0.056
7.242GluLeu: 7.242 ± 0.102
1.314GluMet: 1.314 ± 0.041
2.032GluAsn: 2.032 ± 0.048
2.546GluPro: 2.546 ± 0.054
3.251GluGln: 3.251 ± 0.06
3.329GluArg: 3.329 ± 0.069
3.097GluSer: 3.097 ± 0.054
3.599GluThr: 3.599 ± 0.059
3.869GluVal: 3.869 ± 0.069
0.694GluTrp: 0.694 ± 0.024
1.43GluTyr: 1.43 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.072PheAla: 3.072 ± 0.06
0.499PheCys: 0.499 ± 0.021
2.111PheAsp: 2.111 ± 0.052
1.751PheGlu: 1.751 ± 0.039
1.635PhePhe: 1.635 ± 0.046
2.998PheGly: 2.998 ± 0.063
0.724PheHis: 0.724 ± 0.028
2.241PheIle: 2.241 ± 0.048
1.173PheLys: 1.173 ± 0.035
4.069PheLeu: 4.069 ± 0.074
0.75PheMet: 0.75 ± 0.029
1.687PheAsn: 1.687 ± 0.045
1.934PhePro: 1.934 ± 0.043
2.13PheGln: 2.13 ± 0.051
1.904PheArg: 1.904 ± 0.043
2.633PheSer: 2.633 ± 0.051
2.217PheThr: 2.217 ± 0.045
2.26PheVal: 2.26 ± 0.043
0.782PheTrp: 0.782 ± 0.034
1.326PheTyr: 1.326 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
5.213GlyAla: 5.213 ± 0.091
0.826GlyCys: 0.826 ± 0.031
3.446GlyAsp: 3.446 ± 0.078
4.085GlyGlu: 4.085 ± 0.078
3.057GlyPhe: 3.057 ± 0.055
5.198GlyGly: 5.198 ± 0.133
1.499GlyHis: 1.499 ± 0.041
5.112GlyIle: 5.112 ± 0.062
2.989GlyLys: 2.989 ± 0.057
9.476GlyLeu: 9.476 ± 0.128
1.63GlyMet: 1.63 ± 0.047
2.665GlyAsn: 2.665 ± 0.102
2.277GlyPro: 2.277 ± 0.055
4.369GlyGln: 4.369 ± 0.077
3.667GlyArg: 3.667 ± 0.07
3.905GlySer: 3.905 ± 0.072
4.033GlyThr: 4.033 ± 0.081
5.068GlyVal: 5.068 ± 0.072
1.202GlyTrp: 1.202 ± 0.038
2.332GlyTyr: 2.332 ± 0.048
0.0GlyXaa: 0.0 ± 0.0
His
1.148HisAla: 1.148 ± 0.032
0.305HisCys: 0.305 ± 0.019
0.896HisAsp: 0.896 ± 0.034
0.871HisGlu: 0.871 ± 0.029
0.773HisPhe: 0.773 ± 0.028
1.294HisGly: 1.294 ± 0.033
0.789HisHis: 0.789 ± 0.034
1.0HisIle: 1.0 ± 0.029
0.545HisLys: 0.545 ± 0.022
2.706HisLeu: 2.706 ± 0.06
0.271HisMet: 0.271 ± 0.016
0.676HisAsn: 0.676 ± 0.025
1.652HisPro: 1.652 ± 0.045
1.529HisGln: 1.529 ± 0.041
1.239HisArg: 1.239 ± 0.033
1.115HisSer: 1.115 ± 0.031
0.929HisThr: 0.929 ± 0.036
0.924HisVal: 0.924 ± 0.029
0.447HisTrp: 0.447 ± 0.02
0.789HisTyr: 0.789 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.934IleAla: 5.934 ± 0.077
0.665IleCys: 0.665 ± 0.024
2.989IleAsp: 2.989 ± 0.053
3.582IleGlu: 3.582 ± 0.066
2.407IlePhe: 2.407 ± 0.06
4.382IleGly: 4.382 ± 0.07
1.369IleHis: 1.369 ± 0.036
3.303IleIle: 3.303 ± 0.067
2.354IleLys: 2.354 ± 0.056
6.825IleLeu: 6.825 ± 0.095
0.889IleMet: 0.889 ± 0.032
2.508IleAsn: 2.508 ± 0.059
3.994IlePro: 3.994 ± 0.068
3.371IleGln: 3.371 ± 0.066
3.27IleArg: 3.27 ± 0.055
3.912IleSer: 3.912 ± 0.066
4.027IleThr: 4.027 ± 0.077
3.781IleVal: 3.781 ± 0.065
0.871IleTrp: 0.871 ± 0.034
1.866IleTyr: 1.866 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
3.216LysAla: 3.216 ± 0.056
0.217LysCys: 0.217 ± 0.015
1.642LysAsp: 1.642 ± 0.045
1.998LysGlu: 1.998 ± 0.057
1.363LysPhe: 1.363 ± 0.037
2.09LysGly: 2.09 ± 0.051
0.675LysHis: 0.675 ± 0.023
2.601LysIle: 2.601 ± 0.052
1.578LysLys: 1.578 ± 0.05
4.281LysLeu: 4.281 ± 0.07
0.698LysMet: 0.698 ± 0.028
1.489LysAsn: 1.489 ± 0.039
2.037LysPro: 2.037 ± 0.043
2.019LysGln: 2.019 ± 0.051
2.006LysArg: 2.006 ± 0.054
2.233LysSer: 2.233 ± 0.05
2.528LysThr: 2.528 ± 0.045
2.56LysVal: 2.56 ± 0.063
0.395LysTrp: 0.395 ± 0.02
0.971LysTyr: 0.971 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
11.575LeuAla: 11.575 ± 0.121
1.011LeuCys: 1.011 ± 0.031
5.9LeuAsp: 5.9 ± 0.084
7.412LeuGlu: 7.412 ± 0.099
3.775LeuPhe: 3.775 ± 0.075
9.572LeuGly: 9.572 ± 0.114
2.035LeuHis: 2.035 ± 0.047
7.061LeuIle: 7.061 ± 0.094
4.797LeuLys: 4.797 ± 0.069
11.559LeuLeu: 11.559 ± 0.143
2.198LeuMet: 2.198 ± 0.045
4.444LeuAsn: 4.444 ± 0.076
6.547LeuPro: 6.547 ± 0.092
5.691LeuGln: 5.691 ± 0.097
5.869LeuArg: 5.869 ± 0.093
7.391LeuSer: 7.391 ± 0.084
7.549LeuThr: 7.549 ± 0.097
8.071LeuVal: 8.071 ± 0.097
1.628LeuTrp: 1.628 ± 0.047
2.676LeuTyr: 2.676 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
2.138MetAla: 2.138 ± 0.054
0.105MetCys: 0.105 ± 0.011
0.816MetAsp: 0.816 ± 0.028
0.917MetGlu: 0.917 ± 0.031
0.564MetPhe: 0.564 ± 0.025
1.569MetGly: 1.569 ± 0.035
0.261MetHis: 0.261 ± 0.017
1.246MetIle: 1.246 ± 0.036
0.726MetLys: 0.726 ± 0.027
1.759MetLeu: 1.759 ± 0.042
0.418MetMet: 0.418 ± 0.022
0.767MetAsn: 0.767 ± 0.026
1.129MetPro: 1.129 ± 0.036
0.83MetGln: 0.83 ± 0.028
0.927MetArg: 0.927 ± 0.029
1.334MetSer: 1.334 ± 0.032
1.519MetThr: 1.519 ± 0.036
1.36MetVal: 1.36 ± 0.034
0.124MetTrp: 0.124 ± 0.01
0.309MetTyr: 0.309 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
2.639AsnAla: 2.639 ± 0.054
0.4AsnCys: 0.4 ± 0.023
1.414AsnAsp: 1.414 ± 0.057
1.36AsnGlu: 1.36 ± 0.04
1.576AsnPhe: 1.576 ± 0.041
2.418AsnGly: 2.418 ± 0.092
0.834AsnHis: 0.834 ± 0.025
2.182AsnIle: 2.182 ± 0.062
1.058AsnLys: 1.058 ± 0.033
5.041AsnLeu: 5.041 ± 0.108
0.518AsnMet: 0.518 ± 0.022
1.322AsnAsn: 1.322 ± 0.052
3.073AsnPro: 3.073 ± 0.072
2.584AsnGln: 2.584 ± 0.063
2.201AsnArg: 2.201 ± 0.052
2.369AsnSer: 2.369 ± 0.065
1.906AsnThr: 1.906 ± 0.054
1.977AsnVal: 1.977 ± 0.051
0.633AsnTrp: 0.633 ± 0.028
1.232AsnTyr: 1.232 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
4.527ProAla: 4.527 ± 0.07
0.402ProCys: 0.402 ± 0.02
3.394ProAsp: 3.394 ± 0.054
4.214ProGlu: 4.214 ± 0.081
1.822ProPhe: 1.822 ± 0.046
4.266ProGly: 4.266 ± 0.064
1.102ProHis: 1.102 ± 0.036
3.282ProIle: 3.282 ± 0.061
1.981ProLys: 1.981 ± 0.047
5.86ProLeu: 5.86 ± 0.079
1.014ProMet: 1.014 ± 0.032
2.292ProAsn: 2.292 ± 0.048
3.392ProPro: 3.392 ± 0.078
3.356ProGln: 3.356 ± 0.06
2.262ProArg: 2.262 ± 0.049
3.091ProSer: 3.091 ± 0.056
3.64ProThr: 3.64 ± 0.074
3.598ProVal: 3.598 ± 0.054
0.78ProTrp: 0.78 ± 0.03
1.459ProTyr: 1.459 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
8.729GlnAla: 8.729 ± 0.123
0.358GlnCys: 0.358 ± 0.019
2.429GlnAsp: 2.429 ± 0.048
3.351GlnGlu: 3.351 ± 0.066
2.035GlnPhe: 2.035 ± 0.039
3.944GlnGly: 3.944 ± 0.061
0.971GlnHis: 0.971 ± 0.034
3.529GlnIle: 3.529 ± 0.059
2.116GlnLys: 2.116 ± 0.051
6.3GlnLeu: 6.3 ± 0.099
1.01GlnMet: 1.01 ± 0.031
1.802GlnAsn: 1.802 ± 0.045
3.101GlnPro: 3.101 ± 0.065
3.664GlnGln: 3.664 ± 0.083
3.474GlnArg: 3.474 ± 0.061
3.108GlnSer: 3.108 ± 0.063
3.571GlnThr: 3.571 ± 0.064
4.207GlnVal: 4.207 ± 0.074
0.796GlnTrp: 0.796 ± 0.028
1.177GlnTyr: 1.177 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
3.657ArgAla: 3.657 ± 0.065
0.503ArgCys: 0.503 ± 0.025
2.67ArgAsp: 2.67 ± 0.052
3.026ArgGlu: 3.026 ± 0.069
2.276ArgPhe: 2.276 ± 0.054
3.222ArgGly: 3.222 ± 0.058
1.164ArgHis: 1.164 ± 0.035
3.286ArgIle: 3.286 ± 0.057
1.903ArgLys: 1.903 ± 0.051
6.388ArgLeu: 6.388 ± 0.084
1.054ArgMet: 1.054 ± 0.034
1.983ArgAsn: 1.983 ± 0.05
2.922ArgPro: 2.922 ± 0.057
3.824ArgGln: 3.824 ± 0.072
3.019ArgArg: 3.019 ± 0.065
2.815ArgSer: 2.815 ± 0.05
2.506ArgThr: 2.506 ± 0.048
3.637ArgVal: 3.637 ± 0.062
0.96ArgTrp: 0.96 ± 0.033
1.878ArgTyr: 1.878 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
3.995SerAla: 3.995 ± 0.064
0.527SerCys: 0.527 ± 0.021
2.741SerAsp: 2.741 ± 0.057
3.129SerGlu: 3.129 ± 0.057
2.22SerPhe: 2.22 ± 0.046
4.669SerGly: 4.669 ± 0.08
1.348SerHis: 1.348 ± 0.037
3.063SerIle: 3.063 ± 0.069
1.861SerLys: 1.861 ± 0.051
7.334SerLeu: 7.334 ± 0.098
1.122SerMet: 1.122 ± 0.031
2.026SerAsn: 2.026 ± 0.066
3.971SerPro: 3.971 ± 0.071
4.005SerGln: 4.005 ± 0.074
3.31SerArg: 3.31 ± 0.056
3.656SerSer: 3.656 ± 0.069
3.039SerThr: 3.039 ± 0.073
3.48SerVal: 3.48 ± 0.056
0.819SerTrp: 0.819 ± 0.029
1.71SerTyr: 1.71 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
5.413ThrAla: 5.413 ± 0.069
0.531ThrCys: 0.531 ± 0.02
2.711ThrAsp: 2.711 ± 0.061
3.102ThrGlu: 3.102 ± 0.057
2.082ThrPhe: 2.082 ± 0.047
4.649ThrGly: 4.649 ± 0.093
1.161ThrHis: 1.161 ± 0.036
3.665ThrIle: 3.665 ± 0.063
1.764ThrLys: 1.764 ± 0.041
7.238ThrLeu: 7.238 ± 0.094
0.902ThrMet: 0.902 ± 0.03
1.932ThrAsn: 1.932 ± 0.06
4.099ThrPro: 4.099 ± 0.071
3.206ThrGln: 3.206 ± 0.062
2.568ThrArg: 2.568 ± 0.05
3.208ThrSer: 3.208 ± 0.058
3.707ThrThr: 3.707 ± 0.099
4.307ThrVal: 4.307 ± 0.067
0.84ThrTrp: 0.84 ± 0.028
1.747ThrTyr: 1.747 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
6.254ValAla: 6.254 ± 0.09
0.638ValCys: 0.638 ± 0.029
3.325ValAsp: 3.325 ± 0.06
3.855ValGlu: 3.855 ± 0.064
2.495ValPhe: 2.495 ± 0.045
5.009ValGly: 5.009 ± 0.08
1.004ValHis: 1.004 ± 0.03
4.784ValIle: 4.784 ± 0.066
2.718ValLys: 2.718 ± 0.059
7.17ValLeu: 7.17 ± 0.093
1.574ValMet: 1.574 ± 0.044
2.751ValAsn: 2.751 ± 0.059
3.033ValPro: 3.033 ± 0.058
3.027ValGln: 3.027 ± 0.063
3.207ValArg: 3.207 ± 0.059
3.964ValSer: 3.964 ± 0.066
4.179ValThr: 4.179 ± 0.075
4.97ValVal: 4.97 ± 0.077
0.845ValTrp: 0.845 ± 0.034
1.706ValTyr: 1.706 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
1.145TrpAla: 1.145 ± 0.037
0.149TrpCys: 0.149 ± 0.014
0.711TrpAsp: 0.711 ± 0.028
0.897TrpGlu: 0.897 ± 0.03
0.659TrpPhe: 0.659 ± 0.027
1.354TrpGly: 1.354 ± 0.041
0.413TrpHis: 0.413 ± 0.021
0.836TrpIle: 0.836 ± 0.03
0.435TrpLys: 0.435 ± 0.02
2.305TrpLeu: 2.305 ± 0.059
0.281TrpMet: 0.281 ± 0.018
0.488TrpAsn: 0.488 ± 0.022
0.684TrpPro: 0.684 ± 0.03
1.636TrpGln: 1.636 ± 0.043
0.907TrpArg: 0.907 ± 0.032
0.891TrpSer: 0.891 ± 0.028
0.639TrpThr: 0.639 ± 0.031
1.094TrpVal: 1.094 ± 0.035
0.307TrpTrp: 0.307 ± 0.022
0.375TrpTyr: 0.375 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.089TyrAla: 2.089 ± 0.047
0.401TyrCys: 0.401 ± 0.019
1.443TyrAsp: 1.443 ± 0.057
1.468TyrGlu: 1.468 ± 0.037
1.295TyrPhe: 1.295 ± 0.042
2.318TyrGly: 2.318 ± 0.058
0.684TyrHis: 0.684 ± 0.027
1.325TyrIle: 1.325 ± 0.038
0.844TyrLys: 0.844 ± 0.031
3.679TyrLeu: 3.679 ± 0.053
0.37TyrMet: 0.37 ± 0.018
0.887TyrAsn: 0.887 ± 0.034
1.784TyrPro: 1.784 ± 0.041
2.008TyrGln: 2.008 ± 0.047
2.023TyrArg: 2.023 ± 0.054
1.583TyrSer: 1.583 ± 0.039
1.372TyrThr: 1.372 ± 0.04
1.707TyrVal: 1.707 ± 0.043
0.533TyrTrp: 0.533 ± 0.023
0.985TyrTyr: 0.985 ± 0.04
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3541 proteins (1047018 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski