Amino acid dipepetide frequency for Rivularia sp. PCC 7116

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.588AlaAla: 6.588 ± 0.076
0.782AlaCys: 0.782 ± 0.019
3.825AlaAsp: 3.825 ± 0.05
4.804AlaGlu: 4.804 ± 0.053
2.919AlaPhe: 2.919 ± 0.039
5.125AlaGly: 5.125 ± 0.078
1.032AlaHis: 1.032 ± 0.021
6.189AlaIle: 6.189 ± 0.063
4.306AlaLys: 4.306 ± 0.043
7.569AlaLeu: 7.569 ± 0.079
1.502AlaMet: 1.502 ± 0.031
3.756AlaAsn: 3.756 ± 0.074
2.484AlaPro: 2.484 ± 0.04
3.573AlaGln: 3.573 ± 0.044
3.326AlaArg: 3.326 ± 0.04
4.963AlaSer: 4.963 ± 0.059
4.278AlaThr: 4.278 ± 0.049
5.249AlaVal: 5.249 ± 0.054
0.893AlaTrp: 0.893 ± 0.025
2.241AlaTyr: 2.241 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
0.58CysAla: 0.58 ± 0.016
0.16CysCys: 0.16 ± 0.008
0.545CysAsp: 0.545 ± 0.017
0.56CysGlu: 0.56 ± 0.017
0.453CysPhe: 0.453 ± 0.015
0.767CysGly: 0.767 ± 0.019
0.239CysHis: 0.239 ± 0.01
0.615CysIle: 0.615 ± 0.016
0.446CysLys: 0.446 ± 0.016
1.119CysLeu: 1.119 ± 0.023
0.163CysMet: 0.163 ± 0.009
0.446CysAsn: 0.446 ± 0.015
0.455CysPro: 0.455 ± 0.015
0.472CysGln: 0.472 ± 0.015
0.464CysArg: 0.464 ± 0.017
0.685CysSer: 0.685 ± 0.018
0.442CysThr: 0.442 ± 0.014
0.563CysVal: 0.563 ± 0.016
0.136CysTrp: 0.136 ± 0.008
0.369CysTyr: 0.369 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.669AspAla: 3.669 ± 0.054
0.539AspCys: 0.539 ± 0.015
2.515AspAsp: 2.515 ± 0.045
3.279AspGlu: 3.279 ± 0.042
2.652AspPhe: 2.652 ± 0.039
3.593AspGly: 3.593 ± 0.067
0.421AspHis: 0.421 ± 0.016
4.207AspIle: 4.207 ± 0.051
3.364AspLys: 3.364 ± 0.05
5.284AspLeu: 5.284 ± 0.058
0.798AspMet: 0.798 ± 0.019
2.903AspAsn: 2.903 ± 0.045
1.536AspPro: 1.536 ± 0.034
0.862AspGln: 0.862 ± 0.024
2.253AspArg: 2.253 ± 0.036
3.692AspSer: 3.692 ± 0.051
2.738AspThr: 2.738 ± 0.057
3.052AspVal: 3.052 ± 0.038
0.813AspTrp: 0.813 ± 0.02
1.965AspTyr: 1.965 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
5.176GluAla: 5.176 ± 0.058
0.493GluCys: 0.493 ± 0.016
3.183GluAsp: 3.183 ± 0.044
4.732GluGlu: 4.732 ± 0.059
2.657GluPhe: 2.657 ± 0.038
3.28GluGly: 3.28 ± 0.047
0.945GluHis: 0.945 ± 0.024
5.304GluIle: 5.304 ± 0.057
4.473GluLys: 4.473 ± 0.057
6.939GluLeu: 6.939 ± 0.068
1.284GluMet: 1.284 ± 0.023
3.777GluAsn: 3.777 ± 0.043
2.206GluPro: 2.206 ± 0.035
3.338GluGln: 3.338 ± 0.051
3.031GluArg: 3.031 ± 0.041
3.821GluSer: 3.821 ± 0.042
3.546GluThr: 3.546 ± 0.052
4.384GluVal: 4.384 ± 0.053
0.805GluTrp: 0.805 ± 0.021
2.049GluTyr: 2.049 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
3.221PheAla: 3.221 ± 0.04
0.548PheCys: 0.548 ± 0.015
2.472PheAsp: 2.472 ± 0.041
2.478PheGlu: 2.478 ± 0.036
1.803PhePhe: 1.803 ± 0.031
3.182PheGly: 3.182 ± 0.05
0.729PheHis: 0.729 ± 0.019
2.804PheIle: 2.804 ± 0.039
1.988PheLys: 1.988 ± 0.033
4.122PheLeu: 4.122 ± 0.048
0.718PheMet: 0.718 ± 0.018
2.205PheAsn: 2.205 ± 0.038
1.758PhePro: 1.758 ± 0.027
1.674PheGln: 1.674 ± 0.029
1.782PheArg: 1.782 ± 0.029
3.323PheSer: 3.323 ± 0.047
2.464PheThr: 2.464 ± 0.038
2.565PheVal: 2.565 ± 0.037
0.646PheTrp: 0.646 ± 0.017
1.448PheTyr: 1.448 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
4.46GlyAla: 4.46 ± 0.069
0.762GlyCys: 0.762 ± 0.023
3.492GlyAsp: 3.492 ± 0.066
4.445GlyGlu: 4.445 ± 0.053
3.111GlyPhe: 3.111 ± 0.038
4.9GlyGly: 4.9 ± 0.105
1.017GlyHis: 1.017 ± 0.025
5.402GlyIle: 5.402 ± 0.054
4.662GlyLys: 4.662 ± 0.06
6.374GlyLeu: 6.374 ± 0.064
1.457GlyMet: 1.457 ± 0.027
3.945GlyAsn: 3.945 ± 0.108
0.995GlyPro: 0.995 ± 0.023
2.1GlyGln: 2.1 ± 0.04
2.851GlyArg: 2.851 ± 0.038
4.239GlySer: 4.239 ± 0.066
3.661GlyThr: 3.661 ± 0.058
4.318GlyVal: 4.318 ± 0.049
0.984GlyTrp: 0.984 ± 0.023
2.281GlyTyr: 2.281 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
0.888HisAla: 0.888 ± 0.021
0.219HisCys: 0.219 ± 0.01
0.669HisAsp: 0.669 ± 0.019
0.837HisGlu: 0.837 ± 0.019
0.733HisPhe: 0.733 ± 0.02
0.964HisGly: 0.964 ± 0.023
0.507HisHis: 0.507 ± 0.019
1.007HisIle: 1.007 ± 0.023
0.874HisLys: 0.874 ± 0.023
1.856HisLeu: 1.856 ± 0.034
0.237HisMet: 0.237 ± 0.01
0.789HisAsn: 0.789 ± 0.021
1.074HisPro: 1.074 ± 0.022
0.982HisGln: 0.982 ± 0.02
0.917HisArg: 0.917 ± 0.02
1.109HisSer: 1.109 ± 0.025
0.765HisThr: 0.765 ± 0.021
0.719HisVal: 0.719 ± 0.018
0.279HisTrp: 0.279 ± 0.014
0.579HisTyr: 0.579 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
6.59IleAla: 6.59 ± 0.064
0.803IleCys: 0.803 ± 0.021
3.997IleAsp: 3.997 ± 0.049
4.835IleGlu: 4.835 ± 0.052
3.072IlePhe: 3.072 ± 0.051
4.694IleGly: 4.694 ± 0.058
1.224IleHis: 1.224 ± 0.025
4.605IleIle: 4.605 ± 0.059
4.135IleLys: 4.135 ± 0.049
6.925IleLeu: 6.925 ± 0.073
0.924IleMet: 0.924 ± 0.02
4.103IleAsn: 4.103 ± 0.055
3.72IlePro: 3.72 ± 0.046
2.955IleGln: 2.955 ± 0.039
3.085IleArg: 3.085 ± 0.04
5.345IleSer: 5.345 ± 0.057
3.926IleThr: 3.926 ± 0.05
4.495IleVal: 4.495 ± 0.044
0.884IleTrp: 0.884 ± 0.023
2.195IleTyr: 2.195 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
4.42LysAla: 4.42 ± 0.054
0.405LysCys: 0.405 ± 0.015
2.793LysAsp: 2.793 ± 0.041
3.559LysGlu: 3.559 ± 0.051
2.269LysPhe: 2.269 ± 0.031
3.2LysGly: 3.2 ± 0.043
0.942LysHis: 0.942 ± 0.022
4.437LysIle: 4.437 ± 0.05
3.386LysLys: 3.386 ± 0.052
6.534LysLeu: 6.534 ± 0.068
1.074LysMet: 1.074 ± 0.026
3.125LysAsn: 3.125 ± 0.046
2.795LysPro: 2.795 ± 0.043
3.303LysGln: 3.303 ± 0.052
2.641LysArg: 2.641 ± 0.04
3.864LysSer: 3.864 ± 0.046
3.237LysThr: 3.237 ± 0.045
3.871LysVal: 3.871 ± 0.043
0.631LysTrp: 0.631 ± 0.018
1.878LysTyr: 1.878 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
8.277LeuAla: 8.277 ± 0.075
0.918LeuCys: 0.918 ± 0.02
5.11LeuAsp: 5.11 ± 0.053
7.304LeuGlu: 7.304 ± 0.076
4.034LeuPhe: 4.034 ± 0.051
6.882LeuGly: 6.882 ± 0.07
1.653LeuHis: 1.653 ± 0.029
7.26LeuIle: 7.26 ± 0.067
6.2LeuLys: 6.2 ± 0.07
10.395LeuLeu: 10.395 ± 0.101
1.906LeuMet: 1.906 ± 0.031
5.238LeuAsn: 5.238 ± 0.057
5.116LeuPro: 5.116 ± 0.057
5.498LeuGln: 5.498 ± 0.054
5.037LeuArg: 5.037 ± 0.048
7.611LeuSer: 7.611 ± 0.064
5.851LeuThr: 5.851 ± 0.054
6.402LeuVal: 6.402 ± 0.053
1.32LeuTrp: 1.32 ± 0.032
2.675LeuTyr: 2.675 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
1.459MetAla: 1.459 ± 0.028
0.127MetCys: 0.127 ± 0.008
0.683MetAsp: 0.683 ± 0.019
0.964MetGlu: 0.964 ± 0.022
0.598MetPhe: 0.598 ± 0.016
1.273MetGly: 1.273 ± 0.028
0.305MetHis: 0.305 ± 0.01
1.089MetIle: 1.089 ± 0.022
1.118MetLys: 1.118 ± 0.025
1.877MetLeu: 1.877 ± 0.03
0.457MetMet: 0.457 ± 0.016
0.972MetAsn: 0.972 ± 0.022
0.856MetPro: 0.856 ± 0.019
0.969MetGln: 0.969 ± 0.02
0.918MetArg: 0.918 ± 0.022
1.314MetSer: 1.314 ± 0.026
1.154MetThr: 1.154 ± 0.025
1.165MetVal: 1.165 ± 0.03
0.172MetTrp: 0.172 ± 0.009
0.38MetTyr: 0.38 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.489AsnAla: 3.489 ± 0.061
0.564AsnCys: 0.564 ± 0.017
2.161AsnAsp: 2.161 ± 0.058
2.263AsnGlu: 2.263 ± 0.032
2.373AsnPhe: 2.373 ± 0.034
3.181AsnGly: 3.181 ± 0.062
0.961AsnHis: 0.961 ± 0.021
4.041AsnIle: 4.041 ± 0.062
2.744AsnLys: 2.744 ± 0.039
6.374AsnLeu: 6.374 ± 0.075
0.719AsnMet: 0.719 ± 0.017
3.211AsnAsn: 3.211 ± 0.057
3.193AsnPro: 3.193 ± 0.042
3.266AsnGln: 3.266 ± 0.046
2.68AsnArg: 2.68 ± 0.032
4.204AsnSer: 4.204 ± 0.058
2.747AsnThr: 2.747 ± 0.045
2.579AsnVal: 2.579 ± 0.042
0.819AsnTrp: 0.819 ± 0.019
1.907AsnTyr: 1.907 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
2.685ProAla: 2.685 ± 0.039
0.336ProCys: 0.336 ± 0.012
2.581ProAsp: 2.581 ± 0.037
3.583ProGlu: 3.583 ± 0.047
1.681ProPhe: 1.681 ± 0.028
2.704ProGly: 2.704 ± 0.048
0.722ProHis: 0.722 ± 0.022
2.908ProIle: 2.908 ± 0.043
2.275ProLys: 2.275 ± 0.038
4.114ProLeu: 4.114 ± 0.056
0.65ProMet: 0.65 ± 0.016
2.172ProAsn: 2.172 ± 0.031
1.88ProPro: 1.88 ± 0.038
2.465ProGln: 2.465 ± 0.039
1.531ProArg: 1.531 ± 0.029
2.85ProSer: 2.85 ± 0.043
2.402ProThr: 2.402 ± 0.042
2.937ProVal: 2.937 ± 0.038
0.487ProTrp: 0.487 ± 0.017
1.157ProTyr: 1.157 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
4.082GlnAla: 4.082 ± 0.052
0.282GlnCys: 0.282 ± 0.014
2.081GlnAsp: 2.081 ± 0.032
3.393GlnGlu: 3.393 ± 0.048
1.661GlnPhe: 1.661 ± 0.032
2.734GlnGly: 2.734 ± 0.037
0.731GlnHis: 0.731 ± 0.019
3.63GlnIle: 3.63 ± 0.046
3.055GlnLys: 3.055 ± 0.04
5.447GlnLeu: 5.447 ± 0.07
0.989GlnMet: 0.989 ± 0.023
2.517GlnAsn: 2.517 ± 0.037
2.198GlnPro: 2.198 ± 0.034
3.249GlnGln: 3.249 ± 0.054
2.456GlnArg: 2.456 ± 0.038
2.814GlnSer: 2.814 ± 0.035
2.676GlnThr: 2.676 ± 0.039
3.304GlnVal: 3.304 ± 0.041
0.553GlnTrp: 0.553 ± 0.018
1.122GlnTyr: 1.122 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
2.817ArgAla: 2.817 ± 0.033
0.453ArgCys: 0.453 ± 0.015
2.356ArgAsp: 2.356 ± 0.039
3.149ArgGlu: 3.149 ± 0.034
2.078ArgPhe: 2.078 ± 0.028
2.824ArgGly: 2.824 ± 0.036
0.776ArgHis: 0.776 ± 0.018
3.37ArgIle: 3.37 ± 0.038
2.764ArgLys: 2.764 ± 0.041
5.245ArgLeu: 5.245 ± 0.056
0.93ArgMet: 0.93 ± 0.022
2.467ArgAsn: 2.467 ± 0.029
1.552ArgPro: 1.552 ± 0.029
2.562ArgGln: 2.562 ± 0.043
2.746ArgArg: 2.746 ± 0.044
2.777ArgSer: 2.777 ± 0.038
2.24ArgThr: 2.24 ± 0.036
3.036ArgVal: 3.036 ± 0.041
0.693ArgTrp: 0.693 ± 0.02
1.779ArgTyr: 1.779 ± 0.028
0.0ArgXaa: 0.0 ± 0.0
Ser
4.528SerAla: 4.528 ± 0.051
0.651SerCys: 0.651 ± 0.018
3.346SerAsp: 3.346 ± 0.047
4.542SerGlu: 4.542 ± 0.048
2.971SerPhe: 2.971 ± 0.039
4.948SerGly: 4.948 ± 0.072
1.192SerHis: 1.192 ± 0.027
4.525SerIle: 4.525 ± 0.046
3.641SerLys: 3.641 ± 0.046
7.477SerLeu: 7.477 ± 0.063
1.246SerMet: 1.246 ± 0.023
3.644SerAsn: 3.644 ± 0.052
3.134SerPro: 3.134 ± 0.044
3.843SerGln: 3.843 ± 0.049
3.158SerArg: 3.158 ± 0.038
5.268SerSer: 5.268 ± 0.067
3.692SerThr: 3.692 ± 0.046
4.171SerVal: 4.171 ± 0.051
0.891SerTrp: 0.891 ± 0.022
2.0SerTyr: 2.0 ± 0.027
0.0SerXaa: 0.0 ± 0.0
Thr
4.442ThrAla: 4.442 ± 0.048
0.427ThrCys: 0.427 ± 0.014
2.645ThrAsp: 2.645 ± 0.045
3.223ThrGlu: 3.223 ± 0.053
2.19ThrPhe: 2.19 ± 0.033
4.043ThrGly: 4.043 ± 0.065
0.833ThrHis: 0.833 ± 0.021
3.949ThrIle: 3.949 ± 0.049
2.753ThrLys: 2.753 ± 0.04
5.687ThrLeu: 5.687 ± 0.057
0.727ThrMet: 0.727 ± 0.02
2.694ThrAsn: 2.694 ± 0.04
3.086ThrPro: 3.086 ± 0.057
2.686ThrGln: 2.686 ± 0.045
2.143ThrArg: 2.143 ± 0.03
3.685ThrSer: 3.685 ± 0.042
3.241ThrThr: 3.241 ± 0.061
4.019ThrVal: 4.019 ± 0.05
0.632ThrTrp: 0.632 ± 0.018
1.543ThrTyr: 1.543 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
5.268ValAla: 5.268 ± 0.056
0.626ValCys: 0.626 ± 0.017
3.5ValAsp: 3.5 ± 0.043
4.764ValGlu: 4.764 ± 0.05
2.617ValPhe: 2.617 ± 0.041
4.228ValGly: 4.228 ± 0.053
0.865ValHis: 0.865 ± 0.021
4.469ValIle: 4.469 ± 0.049
3.85ValLys: 3.85 ± 0.053
6.034ValLeu: 6.034 ± 0.055
1.284ValMet: 1.284 ± 0.025
3.429ValAsn: 3.429 ± 0.045
2.496ValPro: 2.496 ± 0.036
2.247ValGln: 2.247 ± 0.035
2.975ValArg: 2.975 ± 0.038
4.386ValSer: 4.386 ± 0.045
3.612ValThr: 3.612 ± 0.054
4.495ValVal: 4.495 ± 0.046
0.779ValTrp: 0.779 ± 0.021
1.832ValTyr: 1.832 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
0.765TrpAla: 0.765 ± 0.018
0.152TrpCys: 0.152 ± 0.008
0.671TrpAsp: 0.671 ± 0.021
0.904TrpGlu: 0.904 ± 0.022
0.592TrpPhe: 0.592 ± 0.02
0.913TrpGly: 0.913 ± 0.024
0.294TrpHis: 0.294 ± 0.011
0.803TrpIle: 0.803 ± 0.02
0.75TrpLys: 0.75 ± 0.021
1.733TrpLeu: 1.733 ± 0.035
0.328TrpMet: 0.328 ± 0.013
0.722TrpAsn: 0.722 ± 0.02
0.12TrpPro: 0.12 ± 0.008
0.955TrpGln: 0.955 ± 0.025
0.712TrpArg: 0.712 ± 0.018
0.718TrpSer: 0.718 ± 0.018
0.522TrpThr: 0.522 ± 0.015
0.856TrpVal: 0.856 ± 0.02
0.234TrpTrp: 0.234 ± 0.011
0.407TrpTyr: 0.407 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.999TyrAla: 1.999 ± 0.03
0.429TyrCys: 0.429 ± 0.014
1.509TyrAsp: 1.509 ± 0.033
1.808TyrGlu: 1.808 ± 0.03
1.467TyrPhe: 1.467 ± 0.026
2.022TyrGly: 2.022 ± 0.035
0.615TyrHis: 0.615 ± 0.017
1.898TyrIle: 1.898 ± 0.032
1.579TyrLys: 1.579 ± 0.032
3.583TyrLeu: 3.583 ± 0.044
0.457TyrMet: 0.457 ± 0.014
1.361TyrAsn: 1.361 ± 0.027
1.465TyrPro: 1.465 ± 0.025
1.973TyrGln: 1.973 ± 0.035
1.908TyrArg: 1.908 ± 0.036
2.201TyrSer: 2.201 ± 0.035
1.457TyrThr: 1.457 ± 0.026
1.576TyrVal: 1.576 ± 0.029
0.501TyrTrp: 0.501 ± 0.018
1.136TyrTyr: 1.136 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6617 proteins (2262525 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski