Mycobacterium phage Send513

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Papyrusvirus; Mycobacterium virus Send513

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1BRM7|G1BRM7_9CAUD Uncharacterized protein OS=Mycobacterium phage Send513 OX=1034146 GN=49 PE=4 SV=1
MM1 pKa = 7.33AAEE4 pKa = 4.3EE5 pKa = 4.56SDD7 pKa = 3.67LQTVYY12 pKa = 11.04DD13 pKa = 4.75CYY15 pKa = 11.29STMLEE20 pKa = 4.09AAGVPGDD27 pKa = 3.95EE28 pKa = 4.11IAWYY32 pKa = 9.09QEE34 pKa = 3.52YY35 pKa = 8.58MANILDD41 pKa = 3.95NVGTQEE47 pKa = 4.6DD48 pKa = 3.83LQEE51 pKa = 4.13VVKK54 pKa = 11.24AMVYY58 pKa = 10.49LKK60 pKa = 10.72GRR62 pKa = 11.84QQ63 pKa = 3.08

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1BRL6|G1BRL6_9CAUD Uncharacterized protein OS=Mycobacterium phage Send513 OX=1034146 GN=38 PE=4 SV=1
MM1 pKa = 7.52ALAGAALNRR10 pKa = 11.84EE11 pKa = 3.93RR12 pKa = 11.84WFEE15 pKa = 3.71LSLRR19 pKa = 11.84LGIFGHH25 pKa = 7.04LSLFVAALCYY35 pKa = 10.06TLFVMVATHH44 pKa = 6.84GPGDD48 pKa = 3.64KK49 pKa = 10.08PYY51 pKa = 8.69WIAVTSVGLSVGIAYY66 pKa = 10.2ASVQRR71 pKa = 11.84FRR73 pKa = 11.84QMRR76 pKa = 11.84KK77 pKa = 9.35LLAEE81 pKa = 3.64YY82 pKa = 10.06RR83 pKa = 11.84RR84 pKa = 11.84RR85 pKa = 11.84EE86 pKa = 3.87RR87 pKa = 11.84EE88 pKa = 3.85RR89 pKa = 11.84NN90 pKa = 3.34

Molecular weight: 10.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

22172

31

1851

226.2

25.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.449 ± 0.426

0.834 ± 0.125

6.517 ± 0.212

6.441 ± 0.236

3.333 ± 0.131

8.209 ± 0.441

2.003 ± 0.141

4.772 ± 0.195

5.444 ± 0.287

7.933 ± 0.16

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.131

4.046 ± 0.134

5.227 ± 0.165

4.014 ± 0.216

6.138 ± 0.348

5.48 ± 0.229

6.179 ± 0.243

6.837 ± 0.216

1.849 ± 0.118

3.044 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski