bacterium SGD-2

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3826 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1UIQ7|A0A6M1UIQ7_9BACT Acyl-CoA carboxylase subunit epsilon OS=bacterium SGD-2 OX=2713607 GN=G5B41_02095 PE=4 SV=1
MM1 pKa = 7.85PIEE4 pKa = 4.12SHH6 pKa = 6.04AVSHH10 pKa = 6.53FEE12 pKa = 4.22LGIVLDD18 pKa = 4.25VVADD22 pKa = 4.24DD23 pKa = 4.49LPLRR27 pKa = 11.84AYY29 pKa = 10.3VAVRR33 pKa = 11.84PPDD36 pKa = 3.99PEE38 pKa = 4.11CVIAFVPPEE47 pKa = 3.89QFEE50 pKa = 4.38NGANLHH56 pKa = 5.25VAAVASTLEE65 pKa = 4.09APGTVEE71 pKa = 5.39DD72 pKa = 3.99VLFNVDD78 pKa = 3.85PNDD81 pKa = 3.56AVAFLCADD89 pKa = 3.69EE90 pKa = 5.37DD91 pKa = 3.55SWLAVLEE98 pKa = 4.01ALGYY102 pKa = 9.71SAGPAPLEE110 pKa = 4.0

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1UK81|A0A6M1UK81_9BACT DUF934 domain-containing protein OS=bacterium SGD-2 OX=2713607 GN=G5B41_06640 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3826

0

3826

1258729

29

2671

329.0

35.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.28 ± 0.055

0.882 ± 0.012

5.489 ± 0.03

5.95 ± 0.042

3.404 ± 0.023

8.326 ± 0.039

2.323 ± 0.018

4.814 ± 0.033

2.746 ± 0.032

10.454 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.446 ± 0.02

2.556 ± 0.023

5.324 ± 0.031

3.596 ± 0.028

7.458 ± 0.048

5.171 ± 0.026

5.197 ± 0.026

7.899 ± 0.033

1.362 ± 0.016

2.323 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski