Amino acid dipepetide frequency for Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.529AlaAla: 5.529 ± 0.144
0.493AlaCys: 0.493 ± 0.028
4.072AlaAsp: 4.072 ± 0.083
3.822AlaGlu: 3.822 ± 0.088
2.981AlaPhe: 2.981 ± 0.083
4.933AlaGly: 4.933 ± 0.103
1.324AlaHis: 1.324 ± 0.053
5.89AlaIle: 5.89 ± 0.146
5.795AlaLys: 5.795 ± 0.16
6.497AlaLeu: 6.497 ± 0.144
1.88AlaMet: 1.88 ± 0.064
3.598AlaAsn: 3.598 ± 0.088
2.086AlaPro: 2.086 ± 0.059
2.904AlaGln: 2.904 ± 0.08
2.608AlaArg: 2.608 ± 0.073
4.062AlaSer: 4.062 ± 0.089
4.185AlaThr: 4.185 ± 0.118
4.781AlaVal: 4.781 ± 0.094
0.664AlaTrp: 0.664 ± 0.034
2.399AlaTyr: 2.399 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.38CysAla: 0.38 ± 0.028
0.043CysCys: 0.043 ± 0.009
0.366CysAsp: 0.366 ± 0.025
0.277CysGlu: 0.277 ± 0.02
0.288CysPhe: 0.288 ± 0.022
0.517CysGly: 0.517 ± 0.031
0.132CysHis: 0.132 ± 0.014
0.373CysIle: 0.373 ± 0.025
0.264CysLys: 0.264 ± 0.024
0.538CysLeu: 0.538 ± 0.03
0.146CysMet: 0.146 ± 0.015
0.229CysAsn: 0.229 ± 0.02
0.243CysPro: 0.243 ± 0.021
0.192CysGln: 0.192 ± 0.019
0.18CysArg: 0.18 ± 0.018
0.36CysSer: 0.36 ± 0.025
0.238CysThr: 0.238 ± 0.019
0.337CysVal: 0.337 ± 0.025
0.077CysTrp: 0.077 ± 0.012
0.231CysTyr: 0.231 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
3.985AspAla: 3.985 ± 0.133
0.317AspCys: 0.317 ± 0.025
3.647AspAsp: 3.647 ± 0.105
4.325AspGlu: 4.325 ± 0.106
2.788AspPhe: 2.788 ± 0.077
3.921AspGly: 3.921 ± 0.101
1.348AspHis: 1.348 ± 0.044
4.255AspIle: 4.255 ± 0.094
4.957AspLys: 4.957 ± 0.111
5.723AspLeu: 5.723 ± 0.126
1.545AspMet: 1.545 ± 0.059
3.366AspAsn: 3.366 ± 0.093
2.673AspPro: 2.673 ± 0.077
2.772AspGln: 2.772 ± 0.09
2.182AspArg: 2.182 ± 0.066
3.204AspSer: 3.204 ± 0.07
2.968AspThr: 2.968 ± 0.093
3.897AspVal: 3.897 ± 0.094
0.716AspTrp: 0.716 ± 0.04
2.706AspTyr: 2.706 ± 0.075
0.0AspXaa: 0.0 ± 0.0
Glu
3.896GluAla: 3.896 ± 0.112
0.24GluCys: 0.24 ± 0.019
3.577GluAsp: 3.577 ± 0.09
3.866GluGlu: 3.866 ± 0.101
2.134GluPhe: 2.134 ± 0.063
2.962GluGly: 2.962 ± 0.07
1.195GluHis: 1.195 ± 0.049
4.565GluIle: 4.565 ± 0.091
5.7GluLys: 5.7 ± 0.107
5.515GluLeu: 5.515 ± 0.119
1.755GluMet: 1.755 ± 0.062
3.887GluAsn: 3.887 ± 0.105
1.709GluPro: 1.709 ± 0.063
2.154GluGln: 2.154 ± 0.067
2.091GluArg: 2.091 ± 0.076
2.599GluSer: 2.599 ± 0.06
2.801GluThr: 2.801 ± 0.073
3.94GluVal: 3.94 ± 0.096
0.49GluTrp: 0.49 ± 0.033
2.01GluTyr: 2.01 ± 0.067
0.0GluXaa: 0.0 ± 0.0
Phe
3.093PheAla: 3.093 ± 0.083
0.317PheCys: 0.317 ± 0.025
2.916PheAsp: 2.916 ± 0.075
2.173PheGlu: 2.173 ± 0.063
2.067PhePhe: 2.067 ± 0.071
2.92PheGly: 2.92 ± 0.096
0.865PheHis: 0.865 ± 0.04
3.348PheIle: 3.348 ± 0.095
3.027PheLys: 3.027 ± 0.083
3.993PheLeu: 3.993 ± 0.115
1.103PheMet: 1.103 ± 0.047
2.572PheAsn: 2.572 ± 0.078
1.336PhePro: 1.336 ± 0.046
1.175PheGln: 1.175 ± 0.045
1.308PheArg: 1.308 ± 0.059
2.937PheSer: 2.937 ± 0.082
2.654PheThr: 2.654 ± 0.069
2.988PheVal: 2.988 ± 0.079
0.58PheTrp: 0.58 ± 0.038
1.717PheTyr: 1.717 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
4.319GlyAla: 4.319 ± 0.117
0.413GlyCys: 0.413 ± 0.026
3.401GlyAsp: 3.401 ± 0.084
3.392GlyGlu: 3.392 ± 0.082
2.873GlyPhe: 2.873 ± 0.087
3.938GlyGly: 3.938 ± 0.099
1.378GlyHis: 1.378 ± 0.048
5.579GlyIle: 5.579 ± 0.122
5.615GlyLys: 5.615 ± 0.1
5.781GlyLeu: 5.781 ± 0.118
1.914GlyMet: 1.914 ± 0.053
3.344GlyAsn: 3.344 ± 0.117
1.524GlyPro: 1.524 ± 0.055
2.581GlyGln: 2.581 ± 0.07
2.36GlyArg: 2.36 ± 0.081
3.825GlySer: 3.825 ± 0.094
3.892GlyThr: 3.892 ± 0.116
4.481GlyVal: 4.481 ± 0.111
0.712GlyTrp: 0.712 ± 0.037
2.894GlyTyr: 2.894 ± 0.092
0.0GlyXaa: 0.0 ± 0.0
His
1.252HisAla: 1.252 ± 0.045
0.113HisCys: 0.113 ± 0.013
1.286HisAsp: 1.286 ± 0.05
1.219HisGlu: 1.219 ± 0.049
1.026HisPhe: 1.026 ± 0.048
1.517HisGly: 1.517 ± 0.06
0.635HisHis: 0.635 ± 0.04
1.337HisIle: 1.337 ± 0.049
1.274HisLys: 1.274 ± 0.044
1.795HisLeu: 1.795 ± 0.064
0.517HisMet: 0.517 ± 0.028
1.113HisAsn: 1.113 ± 0.045
0.979HisPro: 0.979 ± 0.038
0.774HisGln: 0.774 ± 0.036
0.798HisArg: 0.798 ± 0.039
1.048HisSer: 1.048 ± 0.045
0.99HisThr: 0.99 ± 0.039
1.334HisVal: 1.334 ± 0.045
0.238HisTrp: 0.238 ± 0.024
0.985HisTyr: 0.985 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
6.105IleAla: 6.105 ± 0.133
0.584IleCys: 0.584 ± 0.035
5.009IleAsp: 5.009 ± 0.108
4.058IleGlu: 4.058 ± 0.086
3.606IlePhe: 3.606 ± 0.106
5.207IleGly: 5.207 ± 0.13
1.349IleHis: 1.349 ± 0.049
6.144IleIle: 6.144 ± 0.165
5.822IleLys: 5.822 ± 0.119
7.019IleLeu: 7.019 ± 0.165
1.937IleMet: 1.937 ± 0.067
4.771IleAsn: 4.771 ± 0.113
3.175IlePro: 3.175 ± 0.096
2.43IleGln: 2.43 ± 0.065
2.519IleArg: 2.519 ± 0.07
4.961IleSer: 4.961 ± 0.12
4.507IleThr: 4.507 ± 0.078
5.353IleVal: 5.353 ± 0.104
0.772IleTrp: 0.772 ± 0.043
2.807IleTyr: 2.807 ± 0.071
0.0IleXaa: 0.0 ± 0.0
Lys
4.99LysAla: 4.99 ± 0.122
0.241LysCys: 0.241 ± 0.023
5.298LysAsp: 5.298 ± 0.12
5.265LysGlu: 5.265 ± 0.107
2.685LysPhe: 2.685 ± 0.076
3.94LysGly: 3.94 ± 0.085
1.598LysHis: 1.598 ± 0.048
6.132LysIle: 6.132 ± 0.112
7.964LysLys: 7.964 ± 0.173
6.817LysLeu: 6.817 ± 0.12
2.762LysMet: 2.762 ± 0.072
5.313LysAsn: 5.313 ± 0.092
2.616LysPro: 2.616 ± 0.071
3.348LysGln: 3.348 ± 0.095
3.235LysArg: 3.235 ± 0.086
4.185LysSer: 4.185 ± 0.09
4.394LysThr: 4.394 ± 0.111
5.057LysVal: 5.057 ± 0.099
0.752LysTrp: 0.752 ± 0.038
3.421LysTyr: 3.421 ± 0.084
0.0LysXaa: 0.0 ± 0.0
Leu
7.223LeuAla: 7.223 ± 0.128
0.514LeuCys: 0.514 ± 0.035
5.353LeuAsp: 5.353 ± 0.106
4.579LeuGlu: 4.579 ± 0.101
3.961LeuPhe: 3.961 ± 0.117
5.875LeuGly: 5.875 ± 0.129
1.741LeuHis: 1.741 ± 0.063
7.341LeuIle: 7.341 ± 0.144
7.065LeuLys: 7.065 ± 0.127
7.937LeuLeu: 7.937 ± 0.184
2.238LeuMet: 2.238 ± 0.073
5.32LeuAsn: 5.32 ± 0.108
3.601LeuPro: 3.601 ± 0.082
2.976LeuGln: 2.976 ± 0.084
3.413LeuArg: 3.413 ± 0.097
6.024LeuSer: 6.024 ± 0.111
5.788LeuThr: 5.788 ± 0.097
6.009LeuVal: 6.009 ± 0.118
0.743LeuTrp: 0.743 ± 0.04
2.784LeuTyr: 2.784 ± 0.072
0.0LeuXaa: 0.0 ± 0.0
Met
2.158MetAla: 2.158 ± 0.062
0.152MetCys: 0.152 ± 0.017
1.426MetAsp: 1.426 ± 0.049
1.175MetGlu: 1.175 ± 0.052
1.012MetPhe: 1.012 ± 0.057
1.697MetGly: 1.697 ± 0.055
0.56MetHis: 0.56 ± 0.033
2.072MetIle: 2.072 ± 0.071
2.223MetLys: 2.223 ± 0.062
2.31MetLeu: 2.31 ± 0.065
0.861MetMet: 0.861 ± 0.042
1.622MetAsn: 1.622 ± 0.051
1.219MetPro: 1.219 ± 0.046
1.139MetGln: 1.139 ± 0.049
1.062MetArg: 1.062 ± 0.049
1.671MetSer: 1.671 ± 0.054
1.673MetThr: 1.673 ± 0.052
1.743MetVal: 1.743 ± 0.061
0.238MetTrp: 0.238 ± 0.02
0.724MetTyr: 0.724 ± 0.036
0.0MetXaa: 0.0 ± 0.0
Asn
3.741AsnAla: 3.741 ± 0.13
0.301AsnCys: 0.301 ± 0.024
3.402AsnAsp: 3.402 ± 0.092
3.322AsnGlu: 3.322 ± 0.074
2.529AsnPhe: 2.529 ± 0.069
4.202AsnGly: 4.202 ± 0.088
1.183AsnHis: 1.183 ± 0.049
4.14AsnIle: 4.14 ± 0.107
4.682AsnLys: 4.682 ± 0.1
5.252AsnLeu: 5.252 ± 0.109
1.493AsnMet: 1.493 ± 0.053
3.812AsnAsn: 3.812 ± 0.12
2.445AsnPro: 2.445 ± 0.074
2.461AsnGln: 2.461 ± 0.065
2.057AsnArg: 2.057 ± 0.059
3.399AsnSer: 3.399 ± 0.086
2.991AsnThr: 2.991 ± 0.09
3.673AsnVal: 3.673 ± 0.098
0.783AsnTrp: 0.783 ± 0.041
2.577AsnTyr: 2.577 ± 0.083
0.0AsnXaa: 0.0 ± 0.0
Pro
2.495ProAla: 2.495 ± 0.063
0.135ProCys: 0.135 ± 0.016
2.724ProAsp: 2.724 ± 0.09
2.87ProGlu: 2.87 ± 0.087
1.646ProPhe: 1.646 ± 0.06
2.176ProGly: 2.176 ± 0.071
0.656ProHis: 0.656 ± 0.037
2.62ProIle: 2.62 ± 0.074
2.661ProLys: 2.661 ± 0.072
2.825ProLeu: 2.825 ± 0.075
0.812ProMet: 0.812 ± 0.034
2.199ProAsn: 2.199 ± 0.07
0.514ProPro: 0.514 ± 0.036
1.32ProGln: 1.32 ± 0.047
1.12ProArg: 1.12 ± 0.043
1.978ProSer: 1.978 ± 0.067
2.325ProThr: 2.325 ± 0.096
2.765ProVal: 2.765 ± 0.087
0.396ProTrp: 0.396 ± 0.027
1.384ProTyr: 1.384 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
2.63GlnAla: 2.63 ± 0.088
0.134GlnCys: 0.134 ± 0.014
2.072GlnAsp: 2.072 ± 0.071
2.247GlnGlu: 2.247 ± 0.06
1.397GlnPhe: 1.397 ± 0.049
2.029GlnGly: 2.029 ± 0.062
0.644GlnHis: 0.644 ± 0.034
3.396GlnIle: 3.396 ± 0.077
3.474GlnLys: 3.474 ± 0.08
3.598GlnLeu: 3.598 ± 0.09
1.175GlnMet: 1.175 ± 0.047
2.432GlnAsn: 2.432 ± 0.069
1.108GlnPro: 1.108 ± 0.047
1.38GlnGln: 1.38 ± 0.047
1.615GlnArg: 1.615 ± 0.054
1.95GlnSer: 1.95 ± 0.07
2.284GlnThr: 2.284 ± 0.077
2.702GlnVal: 2.702 ± 0.072
0.32GlnTrp: 0.32 ± 0.024
1.438GlnTyr: 1.438 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
2.366ArgAla: 2.366 ± 0.077
0.188ArgCys: 0.188 ± 0.017
2.128ArgAsp: 2.128 ± 0.065
2.295ArgGlu: 2.295 ± 0.086
1.616ArgPhe: 1.616 ± 0.056
2.091ArgGly: 2.091 ± 0.059
0.884ArgHis: 0.884 ± 0.048
2.952ArgIle: 2.952 ± 0.091
3.103ArgLys: 3.103 ± 0.08
3.327ArgLeu: 3.327 ± 0.087
1.096ArgMet: 1.096 ± 0.043
1.961ArgAsn: 1.961 ± 0.054
1.327ArgPro: 1.327 ± 0.047
1.531ArgGln: 1.531 ± 0.052
1.935ArgArg: 1.935 ± 0.073
1.978ArgSer: 1.978 ± 0.056
1.759ArgThr: 1.759 ± 0.054
2.479ArgVal: 2.479 ± 0.07
0.274ArgTrp: 0.274 ± 0.023
1.664ArgTyr: 1.664 ± 0.06
0.0ArgXaa: 0.0 ± 0.0
Ser
3.971SerAla: 3.971 ± 0.098
0.241SerCys: 0.241 ± 0.022
3.741SerAsp: 3.741 ± 0.087
3.24SerGlu: 3.24 ± 0.082
2.759SerPhe: 2.759 ± 0.076
4.416SerGly: 4.416 ± 0.116
1.123SerHis: 1.123 ± 0.048
4.358SerIle: 4.358 ± 0.09
4.161SerLys: 4.161 ± 0.092
5.365SerLeu: 5.365 ± 0.112
1.459SerMet: 1.459 ± 0.056
3.303SerAsn: 3.303 ± 0.09
1.704SerPro: 1.704 ± 0.06
2.248SerGln: 2.248 ± 0.058
2.135SerArg: 2.135 ± 0.062
4.082SerSer: 4.082 ± 0.126
3.507SerThr: 3.507 ± 0.099
3.851SerVal: 3.851 ± 0.09
0.731SerTrp: 0.731 ± 0.04
2.515SerTyr: 2.515 ± 0.076
0.0SerXaa: 0.0 ± 0.0
Thr
4.127ThrAla: 4.127 ± 0.105
0.286ThrCys: 0.286 ± 0.026
3.628ThrAsp: 3.628 ± 0.121
2.882ThrGlu: 2.882 ± 0.069
2.432ThrPhe: 2.432 ± 0.074
4.202ThrGly: 4.202 ± 0.12
1.015ThrHis: 1.015 ± 0.036
4.911ThrIle: 4.911 ± 0.102
4.183ThrLys: 4.183 ± 0.099
4.932ThrLeu: 4.932 ± 0.092
1.337ThrMet: 1.337 ± 0.05
3.13ThrAsn: 3.13 ± 0.106
2.824ThrPro: 2.824 ± 0.166
2.019ThrGln: 2.019 ± 0.077
1.866ThrArg: 1.866 ± 0.065
3.474ThrSer: 3.474 ± 0.105
3.844ThrThr: 3.844 ± 0.146
4.07ThrVal: 4.07 ± 0.11
0.598ThrTrp: 0.598 ± 0.035
2.151ThrTyr: 2.151 ± 0.081
0.0ThrXaa: 0.0 ± 0.0
Val
5.175ValAla: 5.175 ± 0.1
0.375ValCys: 0.375 ± 0.027
4.111ValAsp: 4.111 ± 0.1
3.635ValGlu: 3.635 ± 0.083
2.748ValPhe: 2.748 ± 0.078
4.414ValGly: 4.414 ± 0.092
1.279ValHis: 1.279 ± 0.059
5.322ValIle: 5.322 ± 0.111
5.262ValLys: 5.262 ± 0.112
6.094ValLeu: 6.094 ± 0.104
1.575ValMet: 1.575 ± 0.047
3.762ValAsn: 3.762 ± 0.085
2.67ValPro: 2.67 ± 0.08
2.281ValGln: 2.281 ± 0.065
2.288ValArg: 2.288 ± 0.068
4.236ValSer: 4.236 ± 0.101
4.433ValThr: 4.433 ± 0.172
5.022ValVal: 5.022 ± 0.12
0.625ValTrp: 0.625 ± 0.033
2.358ValTyr: 2.358 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.623TrpAla: 0.623 ± 0.034
0.057TrpCys: 0.057 ± 0.01
0.587TrpAsp: 0.587 ± 0.034
0.399TrpGlu: 0.399 ± 0.028
0.498TrpPhe: 0.498 ± 0.032
0.738TrpGly: 0.738 ± 0.034
0.271TrpHis: 0.271 ± 0.023
0.815TrpIle: 0.815 ± 0.038
0.541TrpLys: 0.541 ± 0.028
1.2TrpLeu: 1.2 ± 0.05
0.307TrpMet: 0.307 ± 0.023
0.606TrpAsn: 0.606 ± 0.033
0.3TrpPro: 0.3 ± 0.022
0.577TrpGln: 0.577 ± 0.034
0.413TrpArg: 0.413 ± 0.028
0.62TrpSer: 0.62 ± 0.035
0.567TrpThr: 0.567 ± 0.041
0.649TrpVal: 0.649 ± 0.041
0.146TrpTrp: 0.146 ± 0.019
0.442TrpTyr: 0.442 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.447TyrAla: 2.447 ± 0.07
0.267TyrCys: 0.267 ± 0.023
2.546TyrAsp: 2.546 ± 0.072
2.046TyrGlu: 2.046 ± 0.061
2.043TyrPhe: 2.043 ± 0.069
2.519TyrGly: 2.519 ± 0.063
1.05TyrHis: 1.05 ± 0.048
2.582TyrIle: 2.582 ± 0.074
2.24TyrLys: 2.24 ± 0.065
3.968TyrLeu: 3.968 ± 0.092
0.824TyrMet: 0.824 ± 0.033
2.106TyrAsn: 2.106 ± 0.052
1.569TyrPro: 1.569 ± 0.058
1.765TyrGln: 1.765 ± 0.068
1.772TyrArg: 1.772 ± 0.055
2.319TyrSer: 2.319 ± 0.059
2.111TyrThr: 2.111 ± 0.075
2.534TyrVal: 2.534 ± 0.066
0.5TyrTrp: 0.5 ± 0.036
1.707TyrTyr: 1.707 ± 0.07
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1859 proteins (583994 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski