Amino acid dipepetide frequency for Staphylococcus phage phi575

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.589AlaAla: 3.589 ± 0.73
0.312AlaCys: 0.312 ± 0.174
2.341AlaAsp: 2.341 ± 0.433
3.667AlaGlu: 3.667 ± 0.523
2.575AlaPhe: 2.575 ± 0.533
3.979AlaGly: 3.979 ± 0.869
0.858AlaHis: 0.858 ± 0.232
3.511AlaIle: 3.511 ± 0.589
5.305AlaLys: 5.305 ± 0.693
5.383AlaLeu: 5.383 ± 0.675
1.56AlaMet: 1.56 ± 0.392
3.277AlaAsn: 3.277 ± 0.54
1.092AlaPro: 1.092 ± 0.268
1.951AlaGln: 1.951 ± 0.458
1.716AlaArg: 1.716 ± 0.474
3.667AlaSer: 3.667 ± 0.686
3.511AlaThr: 3.511 ± 0.506
2.341AlaVal: 2.341 ± 0.492
0.78AlaTrp: 0.78 ± 0.276
2.029AlaTyr: 2.029 ± 0.374
0.0AlaXaa: 0.0 ± 0.0
Cys
0.312CysAla: 0.312 ± 0.184
0.078CysCys: 0.078 ± 0.07
0.546CysAsp: 0.546 ± 0.252
0.624CysGlu: 0.624 ± 0.234
0.078CysPhe: 0.078 ± 0.071
0.468CysGly: 0.468 ± 0.206
0.234CysHis: 0.234 ± 0.167
0.858CysIle: 0.858 ± 0.288
0.858CysLys: 0.858 ± 0.381
0.624CysLeu: 0.624 ± 0.214
0.078CysMet: 0.078 ± 0.08
0.468CysAsn: 0.468 ± 0.301
0.078CysPro: 0.078 ± 0.095
0.078CysGln: 0.078 ± 0.095
0.078CysArg: 0.078 ± 0.092
0.312CysSer: 0.312 ± 0.161
0.39CysThr: 0.39 ± 0.171
0.39CysVal: 0.39 ± 0.16
0.0CysTrp: 0.0 ± 0.0
0.39CysTyr: 0.39 ± 0.182
0.0CysXaa: 0.0 ± 0.0
Asp
2.731AspAla: 2.731 ± 0.426
0.234AspCys: 0.234 ± 0.159
3.433AspAsp: 3.433 ± 0.735
6.242AspGlu: 6.242 ± 0.941
2.965AspPhe: 2.965 ± 0.378
3.901AspGly: 3.901 ± 0.62
0.936AspHis: 0.936 ± 0.31
5.774AspIle: 5.774 ± 0.572
6.008AspLys: 6.008 ± 0.563
3.433AspLeu: 3.433 ± 0.52
0.936AspMet: 0.936 ± 0.241
3.511AspAsn: 3.511 ± 0.493
1.092AspPro: 1.092 ± 0.317
1.482AspGln: 1.482 ± 0.325
1.56AspArg: 1.56 ± 0.483
4.369AspSer: 4.369 ± 0.622
2.731AspThr: 2.731 ± 0.623
3.745AspVal: 3.745 ± 0.729
0.702AspTrp: 0.702 ± 0.222
3.901AspTyr: 3.901 ± 0.631
0.0AspXaa: 0.0 ± 0.0
Glu
3.667GluAla: 3.667 ± 0.561
0.546GluCys: 0.546 ± 0.203
4.135GluAsp: 4.135 ± 0.53
7.724GluGlu: 7.724 ± 1.208
2.965GluPhe: 2.965 ± 0.419
2.341GluGly: 2.341 ± 0.443
1.638GluHis: 1.638 ± 0.383
6.32GluIle: 6.32 ± 1.218
6.632GluLys: 6.632 ± 0.744
8.192GluLeu: 8.192 ± 1.069
2.185GluMet: 2.185 ± 0.386
5.149GluAsn: 5.149 ± 0.896
2.029GluPro: 2.029 ± 0.434
4.135GluGln: 4.135 ± 0.686
3.043GluArg: 3.043 ± 0.607
4.447GluSer: 4.447 ± 0.612
3.511GluThr: 3.511 ± 0.539
5.305GluVal: 5.305 ± 0.648
0.702GluTrp: 0.702 ± 0.209
3.121GluTyr: 3.121 ± 0.648
0.0GluXaa: 0.0 ± 0.0
Phe
1.716PheAla: 1.716 ± 0.407
0.39PheCys: 0.39 ± 0.139
2.107PheAsp: 2.107 ± 0.355
3.277PheGlu: 3.277 ± 0.421
1.638PhePhe: 1.638 ± 0.372
3.667PheGly: 3.667 ± 0.546
0.546PheHis: 0.546 ± 0.208
3.355PheIle: 3.355 ± 0.486
5.461PheLys: 5.461 ± 0.683
2.809PheLeu: 2.809 ± 0.494
1.56PheMet: 1.56 ± 0.363
3.589PheAsn: 3.589 ± 0.473
0.702PhePro: 0.702 ± 0.214
1.326PheGln: 1.326 ± 0.359
1.873PheArg: 1.873 ± 0.369
2.653PheSer: 2.653 ± 0.627
2.341PheThr: 2.341 ± 0.424
1.716PheVal: 1.716 ± 0.408
0.39PheTrp: 0.39 ± 0.149
1.404PheTyr: 1.404 ± 0.376
0.0PheXaa: 0.0 ± 0.0
Gly
2.419GlyAla: 2.419 ± 0.783
0.156GlyCys: 0.156 ± 0.121
3.511GlyAsp: 3.511 ± 0.675
2.263GlyGlu: 2.263 ± 0.416
3.823GlyPhe: 3.823 ± 0.68
3.277GlyGly: 3.277 ± 0.815
0.858GlyHis: 0.858 ± 0.245
4.603GlyIle: 4.603 ± 0.78
5.696GlyLys: 5.696 ± 0.723
4.603GlyLeu: 4.603 ± 0.615
1.326GlyMet: 1.326 ± 0.55
3.433GlyAsn: 3.433 ± 0.515
1.326GlyPro: 1.326 ± 0.478
2.107GlyGln: 2.107 ± 0.374
2.185GlyArg: 2.185 ± 0.447
3.199GlySer: 3.199 ± 0.777
4.369GlyThr: 4.369 ± 0.7
4.135GlyVal: 4.135 ± 0.714
0.858GlyTrp: 0.858 ± 0.255
2.809GlyTyr: 2.809 ± 0.415
0.0GlyXaa: 0.0 ± 0.0
His
1.404HisAla: 1.404 ± 0.346
0.234HisCys: 0.234 ± 0.17
1.014HisAsp: 1.014 ± 0.28
1.326HisGlu: 1.326 ± 0.288
0.936HisPhe: 0.936 ± 0.213
1.248HisGly: 1.248 ± 0.284
0.312HisHis: 0.312 ± 0.144
1.17HisIle: 1.17 ± 0.333
1.638HisLys: 1.638 ± 0.402
1.248HisLeu: 1.248 ± 0.345
0.702HisMet: 0.702 ± 0.19
1.248HisAsn: 1.248 ± 0.294
0.546HisPro: 0.546 ± 0.269
0.234HisGln: 0.234 ± 0.153
1.014HisArg: 1.014 ± 0.317
0.936HisSer: 0.936 ± 0.352
0.936HisThr: 0.936 ± 0.254
1.326HisVal: 1.326 ± 0.382
0.234HisTrp: 0.234 ± 0.111
0.702HisTyr: 0.702 ± 0.22
0.0HisXaa: 0.0 ± 0.0
Ile
4.447IleAla: 4.447 ± 0.642
0.312IleCys: 0.312 ± 0.14
5.149IleAsp: 5.149 ± 0.781
6.632IleGlu: 6.632 ± 0.854
2.887IlePhe: 2.887 ± 0.55
4.213IleGly: 4.213 ± 0.679
1.482IleHis: 1.482 ± 0.305
5.461IleIle: 5.461 ± 0.674
9.129IleLys: 9.129 ± 0.878
5.305IleLeu: 5.305 ± 0.736
1.17IleMet: 1.17 ± 0.333
5.383IleAsn: 5.383 ± 0.691
2.107IlePro: 2.107 ± 0.362
2.653IleGln: 2.653 ± 0.37
2.731IleArg: 2.731 ± 0.609
4.915IleSer: 4.915 ± 0.589
4.447IleThr: 4.447 ± 0.605
5.071IleVal: 5.071 ± 0.592
1.014IleTrp: 1.014 ± 0.388
2.731IleTyr: 2.731 ± 0.432
0.0IleXaa: 0.0 ± 0.0
Lys
4.837LysAla: 4.837 ± 0.8
0.312LysCys: 0.312 ± 0.215
6.554LysAsp: 6.554 ± 0.874
7.646LysGlu: 7.646 ± 0.868
3.823LysPhe: 3.823 ± 0.492
5.383LysGly: 5.383 ± 1.122
2.419LysHis: 2.419 ± 0.638
5.774LysIle: 5.774 ± 0.757
8.972LysLys: 8.972 ± 1.067
9.363LysLeu: 9.363 ± 0.85
2.731LysMet: 2.731 ± 0.507
5.071LysAsn: 5.071 ± 0.488
2.341LysPro: 2.341 ± 0.436
3.745LysGln: 3.745 ± 0.489
4.759LysArg: 4.759 ± 0.82
7.022LysSer: 7.022 ± 0.988
5.93LysThr: 5.93 ± 0.771
5.852LysVal: 5.852 ± 0.599
0.624LysTrp: 0.624 ± 0.218
5.071LysTyr: 5.071 ± 0.773
0.0LysXaa: 0.0 ± 0.0
Leu
3.589LeuAla: 3.589 ± 0.572
0.858LeuCys: 0.858 ± 0.362
4.681LeuAsp: 4.681 ± 0.67
5.227LeuGlu: 5.227 ± 0.69
3.043LeuPhe: 3.043 ± 0.394
4.057LeuGly: 4.057 ± 0.676
1.248LeuHis: 1.248 ± 0.362
6.398LeuIle: 6.398 ± 0.78
8.738LeuLys: 8.738 ± 1.074
7.256LeuLeu: 7.256 ± 1.034
1.951LeuMet: 1.951 ± 0.356
6.476LeuAsn: 6.476 ± 0.647
2.497LeuPro: 2.497 ± 0.37
3.043LeuGln: 3.043 ± 0.453
3.355LeuArg: 3.355 ± 0.515
6.164LeuSer: 6.164 ± 0.859
5.54LeuThr: 5.54 ± 0.885
4.993LeuVal: 4.993 ± 0.549
0.546LeuTrp: 0.546 ± 0.191
3.589LeuTyr: 3.589 ± 0.634
0.0LeuXaa: 0.0 ± 0.0
Met
1.404MetAla: 1.404 ± 0.425
0.234MetCys: 0.234 ± 0.148
1.014MetAsp: 1.014 ± 0.232
1.248MetGlu: 1.248 ± 0.315
0.78MetPhe: 0.78 ± 0.23
0.858MetGly: 0.858 ± 0.329
0.702MetHis: 0.702 ± 0.23
1.56MetIle: 1.56 ± 0.31
2.653MetLys: 2.653 ± 0.459
2.107MetLeu: 2.107 ± 0.436
0.468MetMet: 0.468 ± 0.181
1.873MetAsn: 1.873 ± 0.376
0.78MetPro: 0.78 ± 0.279
1.014MetGln: 1.014 ± 0.321
1.248MetArg: 1.248 ± 0.33
2.185MetSer: 2.185 ± 0.488
2.029MetThr: 2.029 ± 0.512
1.638MetVal: 1.638 ± 0.343
0.078MetTrp: 0.078 ± 0.066
1.17MetTyr: 1.17 ± 0.343
0.0MetXaa: 0.0 ± 0.0
Asn
3.745AsnAla: 3.745 ± 0.547
0.312AsnCys: 0.312 ± 0.144
4.369AsnAsp: 4.369 ± 0.751
5.618AsnGlu: 5.618 ± 0.752
2.653AsnPhe: 2.653 ± 0.373
5.227AsnGly: 5.227 ± 0.847
1.404AsnHis: 1.404 ± 0.375
5.774AsnIle: 5.774 ± 0.631
7.646AsnLys: 7.646 ± 0.885
5.071AsnLeu: 5.071 ± 0.505
1.716AsnMet: 1.716 ± 0.344
4.213AsnAsn: 4.213 ± 0.702
2.263AsnPro: 2.263 ± 0.318
2.731AsnGln: 2.731 ± 0.617
2.263AsnArg: 2.263 ± 0.386
3.355AsnSer: 3.355 ± 0.62
2.731AsnThr: 2.731 ± 0.444
2.575AsnVal: 2.575 ± 0.516
0.78AsnTrp: 0.78 ± 0.239
2.419AsnTyr: 2.419 ± 0.422
0.0AsnXaa: 0.0 ± 0.0
Pro
1.482ProAla: 1.482 ± 0.36
0.0ProCys: 0.0 ± 0.0
1.873ProAsp: 1.873 ± 0.42
2.263ProGlu: 2.263 ± 0.514
1.17ProPhe: 1.17 ± 0.417
1.638ProGly: 1.638 ± 0.469
0.546ProHis: 0.546 ± 0.209
2.575ProIle: 2.575 ± 0.376
2.341ProLys: 2.341 ± 0.357
2.029ProLeu: 2.029 ± 0.487
0.546ProMet: 0.546 ± 0.253
1.873ProAsn: 1.873 ± 0.34
0.468ProPro: 0.468 ± 0.159
0.702ProGln: 0.702 ± 0.218
0.546ProArg: 0.546 ± 0.258
2.107ProSer: 2.107 ± 0.373
1.404ProThr: 1.404 ± 0.38
1.56ProVal: 1.56 ± 0.286
0.156ProTrp: 0.156 ± 0.133
0.78ProTyr: 0.78 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
2.419GlnAla: 2.419 ± 0.488
0.156GlnCys: 0.156 ± 0.116
2.029GlnAsp: 2.029 ± 0.365
3.277GlnGlu: 3.277 ± 0.596
1.17GlnPhe: 1.17 ± 0.351
1.404GlnGly: 1.404 ± 0.28
0.39GlnHis: 0.39 ± 0.151
3.667GlnIle: 3.667 ± 0.537
2.185GlnLys: 2.185 ± 0.544
3.277GlnLeu: 3.277 ± 0.612
1.092GlnMet: 1.092 ± 0.284
2.263GlnAsn: 2.263 ± 0.454
0.624GlnPro: 0.624 ± 0.247
2.185GlnGln: 2.185 ± 0.496
2.185GlnArg: 2.185 ± 0.39
2.497GlnSer: 2.497 ± 0.481
1.248GlnThr: 1.248 ± 0.362
2.263GlnVal: 2.263 ± 0.465
0.39GlnTrp: 0.39 ± 0.154
2.107GlnTyr: 2.107 ± 0.418
0.0GlnXaa: 0.0 ± 0.0
Arg
2.029ArgAla: 2.029 ± 0.541
0.234ArgCys: 0.234 ± 0.189
2.497ArgAsp: 2.497 ± 0.484
2.731ArgGlu: 2.731 ± 0.467
1.716ArgPhe: 1.716 ± 0.368
1.716ArgGly: 1.716 ± 0.316
0.936ArgHis: 0.936 ± 0.21
3.511ArgIle: 3.511 ± 0.682
3.667ArgLys: 3.667 ± 0.535
3.043ArgLeu: 3.043 ± 0.45
1.638ArgMet: 1.638 ± 0.31
3.355ArgAsn: 3.355 ± 0.463
1.248ArgPro: 1.248 ± 0.354
1.248ArgGln: 1.248 ± 0.225
1.873ArgArg: 1.873 ± 0.481
2.185ArgSer: 2.185 ± 0.457
1.951ArgThr: 1.951 ± 0.367
2.419ArgVal: 2.419 ± 0.417
0.546ArgTrp: 0.546 ± 0.176
1.482ArgTyr: 1.482 ± 0.319
0.0ArgXaa: 0.0 ± 0.0
Ser
3.277SerAla: 3.277 ± 0.593
0.702SerCys: 0.702 ± 0.263
2.809SerAsp: 2.809 ± 0.441
5.696SerGlu: 5.696 ± 0.772
2.887SerPhe: 2.887 ± 0.488
4.603SerGly: 4.603 ± 0.801
1.17SerHis: 1.17 ± 0.322
4.291SerIle: 4.291 ± 0.608
6.866SerLys: 6.866 ± 0.974
5.461SerLeu: 5.461 ± 0.751
2.107SerMet: 2.107 ± 0.329
4.525SerAsn: 4.525 ± 0.51
1.56SerPro: 1.56 ± 0.326
2.497SerGln: 2.497 ± 0.622
2.809SerArg: 2.809 ± 0.404
3.589SerSer: 3.589 ± 0.65
3.589SerThr: 3.589 ± 0.449
2.653SerVal: 2.653 ± 0.295
0.936SerTrp: 0.936 ± 0.246
1.951SerTyr: 1.951 ± 0.524
0.0SerXaa: 0.0 ± 0.0
Thr
3.277ThrAla: 3.277 ± 0.342
0.546ThrCys: 0.546 ± 0.216
4.057ThrAsp: 4.057 ± 0.66
3.901ThrGlu: 3.901 ± 0.683
2.731ThrPhe: 2.731 ± 0.359
3.433ThrGly: 3.433 ± 0.9
1.014ThrHis: 1.014 ± 0.258
3.667ThrIle: 3.667 ± 0.412
5.227ThrLys: 5.227 ± 0.581
5.149ThrLeu: 5.149 ± 0.607
1.014ThrMet: 1.014 ± 0.246
3.511ThrAsn: 3.511 ± 0.751
2.263ThrPro: 2.263 ± 0.428
1.794ThrGln: 1.794 ± 0.371
2.263ThrArg: 2.263 ± 0.496
3.901ThrSer: 3.901 ± 0.742
3.667ThrThr: 3.667 ± 0.609
2.809ThrVal: 2.809 ± 0.439
0.78ThrTrp: 0.78 ± 0.305
2.029ThrTyr: 2.029 ± 0.483
0.0ThrXaa: 0.0 ± 0.0
Val
4.057ValAla: 4.057 ± 0.548
0.39ValCys: 0.39 ± 0.176
4.213ValAsp: 4.213 ± 0.56
4.135ValGlu: 4.135 ± 0.737
2.575ValPhe: 2.575 ± 0.471
3.199ValGly: 3.199 ± 0.617
0.546ValHis: 0.546 ± 0.173
4.915ValIle: 4.915 ± 0.631
4.681ValLys: 4.681 ± 0.639
4.213ValLeu: 4.213 ± 0.664
1.326ValMet: 1.326 ± 0.292
4.681ValAsn: 4.681 ± 0.536
1.326ValPro: 1.326 ± 0.365
1.873ValGln: 1.873 ± 0.324
2.575ValArg: 2.575 ± 0.727
2.965ValSer: 2.965 ± 0.533
3.823ValThr: 3.823 ± 0.602
3.901ValVal: 3.901 ± 0.65
0.39ValTrp: 0.39 ± 0.207
1.951ValTyr: 1.951 ± 0.459
0.0ValXaa: 0.0 ± 0.0
Trp
0.546TrpAla: 0.546 ± 0.335
0.078TrpCys: 0.078 ± 0.072
0.546TrpAsp: 0.546 ± 0.189
0.39TrpGlu: 0.39 ± 0.176
0.312TrpPhe: 0.312 ± 0.184
0.312TrpGly: 0.312 ± 0.134
0.312TrpHis: 0.312 ± 0.162
0.702TrpIle: 0.702 ± 0.218
1.014TrpLys: 1.014 ± 0.275
1.716TrpLeu: 1.716 ± 0.302
0.234TrpMet: 0.234 ± 0.132
0.39TrpAsn: 0.39 ± 0.265
0.234TrpPro: 0.234 ± 0.113
0.312TrpGln: 0.312 ± 0.23
0.468TrpArg: 0.468 ± 0.21
0.936TrpSer: 0.936 ± 0.361
0.78TrpThr: 0.78 ± 0.195
0.546TrpVal: 0.546 ± 0.198
0.234TrpTrp: 0.234 ± 0.132
0.312TrpTyr: 0.312 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.497TyrAla: 2.497 ± 0.384
0.858TyrCys: 0.858 ± 0.393
2.809TyrAsp: 2.809 ± 0.436
3.901TyrGlu: 3.901 ± 0.578
1.794TyrPhe: 1.794 ± 0.482
2.107TyrGly: 2.107 ± 0.399
0.858TyrHis: 0.858 ± 0.257
3.355TyrIle: 3.355 ± 0.591
3.511TyrLys: 3.511 ± 0.482
2.965TyrLeu: 2.965 ± 0.648
0.624TyrMet: 0.624 ± 0.252
2.575TyrAsn: 2.575 ± 0.433
1.482TyrPro: 1.482 ± 0.334
1.716TyrGln: 1.716 ± 0.332
1.482TyrArg: 1.482 ± 0.415
2.575TyrSer: 2.575 ± 0.398
2.107TyrThr: 2.107 ± 0.409
2.497TyrVal: 2.497 ± 0.449
0.234TyrTrp: 0.234 ± 0.144
1.638TyrTyr: 1.638 ± 0.478
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12818 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski