Tortoise microvirus 30

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W722|A0A4P8W722_9VIRU DNA pilot protein OS=Tortoise microvirus 30 OX=2583133 PE=4 SV=1
MM1 pKa = 7.65LIKK4 pKa = 9.33EE5 pKa = 4.62TKK7 pKa = 9.6LKK9 pKa = 10.72RR10 pKa = 11.84KK11 pKa = 9.76IITQNDD17 pKa = 3.62LQEE20 pKa = 4.34VAEE23 pKa = 4.51SLLNDD28 pKa = 3.86KK29 pKa = 9.34VTDD32 pKa = 3.59PTGYY36 pKa = 9.61IPLRR40 pKa = 11.84NRR42 pKa = 11.84IEE44 pKa = 4.0AFEE47 pKa = 4.14AAGQNLEE54 pKa = 3.86EE55 pKa = 4.34WRR57 pKa = 11.84DD58 pKa = 3.47QNFDD62 pKa = 3.66YY63 pKa = 10.92EE64 pKa = 4.43SWEE67 pKa = 3.9EE68 pKa = 3.56EE69 pKa = 4.54DD70 pKa = 4.98IIEE73 pKa = 4.24EE74 pKa = 4.05NMNLGQYY81 pKa = 10.36AIRR84 pKa = 11.84RR85 pKa = 11.84QGSDD89 pKa = 2.12IVEE92 pKa = 3.95ILNEE96 pKa = 3.69QEE98 pKa = 3.62AAYY101 pKa = 10.41FNLLRR106 pKa = 11.84QTKK109 pKa = 7.42QPRR112 pKa = 11.84RR113 pKa = 11.84RR114 pKa = 11.84DD115 pKa = 3.32LWEE118 pKa = 4.27EE119 pKa = 3.92EE120 pKa = 4.79ADD122 pKa = 5.51DD123 pKa = 5.08GDD125 pKa = 4.32PKK127 pKa = 10.91QGADD131 pKa = 3.55SKK133 pKa = 11.35KK134 pKa = 8.13EE135 pKa = 3.91HH136 pKa = 6.54QDD138 pKa = 3.23QSHH141 pKa = 6.45TDD143 pKa = 2.92SGTDD147 pKa = 3.13KK148 pKa = 11.17KK149 pKa = 9.95EE150 pKa = 3.96QKK152 pKa = 10.34DD153 pKa = 3.41

Molecular weight: 18.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9K2|A0A4P8W9K2_9VIRU Major capsid protein OS=Tortoise microvirus 30 OX=2583133 PE=3 SV=1
MM1 pKa = 6.29TCNRR5 pKa = 11.84KK6 pKa = 9.35FYY8 pKa = 10.1INKK11 pKa = 9.2VLVPCGKK18 pKa = 10.6CIACKK23 pKa = 10.1KK24 pKa = 9.55NKK26 pKa = 8.04SHH28 pKa = 6.17EE29 pKa = 3.95WAKK32 pKa = 10.62RR33 pKa = 11.84ISDD36 pKa = 4.7EE37 pKa = 4.34IFCSDD42 pKa = 4.11GQCSFITLTYY52 pKa = 10.33KK53 pKa = 10.6EE54 pKa = 4.12NNKK57 pKa = 9.63AQIYY61 pKa = 9.7SLSIDD66 pKa = 5.05DD67 pKa = 4.23IQKK70 pKa = 8.72FWKK73 pKa = 9.96RR74 pKa = 11.84LRR76 pKa = 11.84KK77 pKa = 9.63KK78 pKa = 10.62FPNKK82 pKa = 9.2KK83 pKa = 9.73FKK85 pKa = 10.77YY86 pKa = 8.65FCCGEE91 pKa = 4.08YY92 pKa = 10.89GEE94 pKa = 4.55QNQRR98 pKa = 11.84PHH100 pKa = 5.5YY101 pKa = 8.59HH102 pKa = 6.96AIIIGLSLKK111 pKa = 10.67DD112 pKa = 3.68LEE114 pKa = 4.63AEE116 pKa = 4.4KK117 pKa = 10.51KK118 pKa = 8.1QHH120 pKa = 6.17SNYY123 pKa = 8.71ATSKK127 pKa = 9.82VLEE130 pKa = 4.35KK131 pKa = 9.78TWPFGFNTVGIATINSIHH149 pKa = 6.04YY150 pKa = 9.26VSGYY154 pKa = 9.19ILKK157 pKa = 7.11TTWGQHH163 pKa = 4.38AVKK166 pKa = 9.92IYY168 pKa = 11.02DD169 pKa = 3.95KK170 pKa = 10.9IGLTRR175 pKa = 11.84PFLITSKK182 pKa = 10.73QLGLKK187 pKa = 8.73YY188 pKa = 10.49LKK190 pKa = 10.1EE191 pKa = 4.07NQEE194 pKa = 4.37MIRR197 pKa = 11.84NTRR200 pKa = 11.84KK201 pKa = 10.18GNPRR205 pKa = 11.84YY206 pKa = 9.61YY207 pKa = 9.73IKK209 pKa = 10.42KK210 pKa = 10.06SKK212 pKa = 10.7EE213 pKa = 3.23IDD215 pKa = 3.46SNINIIKK222 pKa = 10.07GSPKK226 pKa = 9.92IISKK230 pKa = 10.95EE231 pKa = 3.78EE232 pKa = 3.86GKK234 pKa = 10.41KK235 pKa = 10.65LKK237 pKa = 10.01TIMTKK242 pKa = 10.37NKK244 pKa = 9.13CNLMEE249 pKa = 4.14ARR251 pKa = 11.84RR252 pKa = 11.84RR253 pKa = 11.84ILEE256 pKa = 4.1SNLARR261 pKa = 11.84EE262 pKa = 4.29QNEE265 pKa = 3.96LAKK268 pKa = 10.4QHH270 pKa = 6.0LLSRR274 pKa = 11.84RR275 pKa = 11.84HH276 pKa = 5.42KK277 pKa = 10.95NIGEE281 pKa = 4.4FII283 pKa = 4.07

Molecular weight: 33.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1320

83

513

264.0

30.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.515 ± 0.928

0.909 ± 0.586

5.455 ± 0.996

7.045 ± 1.492

3.864 ± 0.401

5.152 ± 0.165

2.273 ± 0.351

8.788 ± 0.572

7.955 ± 1.934

7.955 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.879 ± 0.512

6.97 ± 0.287

3.712 ± 1.233

5.682 ± 0.732

5.833 ± 0.359

4.924 ± 0.56

6.212 ± 0.55

2.803 ± 0.566

1.667 ± 0.252

3.409 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski