Thermoactinomyces sp. AS95

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Thermoactinomyces; unclassified Thermoactinomyces

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2550 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A151YYF4|A0A151YYF4_9BACL Putative pre-16S rRNA nuclease OS=Thermoactinomyces sp. AS95 OX=1811386 GN=AYX07_06225 PE=3 SV=1
MM1 pKa = 7.39AHH3 pKa = 5.39NHH5 pKa = 5.09EE6 pKa = 4.67PEE8 pKa = 4.07YY9 pKa = 11.02LIIPTEE15 pKa = 3.91NGKK18 pKa = 8.43EE19 pKa = 3.95EE20 pKa = 4.18KK21 pKa = 10.77FEE23 pKa = 3.77ILYY26 pKa = 9.29TFDD29 pKa = 3.52HH30 pKa = 7.34DD31 pKa = 4.24EE32 pKa = 4.2TGKK35 pKa = 10.87SYY37 pKa = 10.55MFVTPVDD44 pKa = 4.11GEE46 pKa = 4.31QGGEE50 pKa = 3.88PDD52 pKa = 3.45EE53 pKa = 4.16QEE55 pKa = 4.42PYY57 pKa = 9.99QEE59 pKa = 4.09VMAFRR64 pKa = 11.84YY65 pKa = 8.67TEE67 pKa = 3.8EE68 pKa = 4.72DD69 pKa = 3.49GNLNLEE75 pKa = 4.46MIEE78 pKa = 4.16EE79 pKa = 4.26EE80 pKa = 5.46NEE82 pKa = 4.19EE83 pKa = 3.96EE84 pKa = 3.73WDD86 pKa = 3.53MVEE89 pKa = 4.56EE90 pKa = 4.23VFNTLVSEE98 pKa = 4.31LEE100 pKa = 4.16EE101 pKa = 3.83EE102 pKa = 4.6SEE104 pKa = 4.59EE105 pKa = 4.38NEE107 pKa = 4.07QQ108 pKa = 4.47

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A151YXM6|A0A151YXM6_9BACL Radical SAM protein OS=Thermoactinomyces sp. AS95 OX=1811386 GN=AYX07_10650 PE=4 SV=1
MM1 pKa = 7.54TIGRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 8.31WLWGGGLLVALILSVSLGVFLGSTDD32 pKa = 3.33VGFWTVWKK40 pKa = 10.7VILQHH45 pKa = 6.34TGLADD50 pKa = 3.9GLNMDD55 pKa = 4.69EE56 pKa = 5.14ADD58 pKa = 4.47AAIIWEE64 pKa = 4.09LRR66 pKa = 11.84LPRR69 pKa = 11.84VLLVGLVGACLATAGVIYY87 pKa = 10.46QGLLKK92 pKa = 10.75NHH94 pKa = 6.74LADD97 pKa = 4.39PYY99 pKa = 10.64ILGVSSGAAVGAALVIITGWGDD121 pKa = 2.97RR122 pKa = 11.84SGWMLPLFAFLGACLALLLVLQLGRR147 pKa = 11.84GRR149 pKa = 11.84RR150 pKa = 11.84ASQASSLILAGVVVQAFFSAVLTLAISTSPEE181 pKa = 3.31EE182 pKa = 3.94MQRR185 pKa = 11.84IQFWLMGSFAFRR197 pKa = 11.84GWQHH201 pKa = 6.38IGLLLPFFLIGFLVCWRR218 pKa = 11.84FSRR221 pKa = 11.84EE222 pKa = 3.9LNLMVLGEE230 pKa = 4.41RR231 pKa = 11.84SAHH234 pKa = 5.88HH235 pKa = 6.48LGIHH239 pKa = 4.75VEE241 pKa = 3.85KK242 pKa = 10.42MRR244 pKa = 11.84IFLLIIVSLMTSAAVSVSGMIGFVGLVVPHH274 pKa = 6.99IMRR277 pKa = 11.84MLTGADD283 pKa = 3.3HH284 pKa = 7.54RR285 pKa = 11.84YY286 pKa = 9.76LLPLSALFGASFLIWADD303 pKa = 3.31GLSRR307 pKa = 11.84IVLAPRR313 pKa = 11.84EE314 pKa = 4.1LPIGVVTAFVGAPFFAGILRR334 pKa = 11.84RR335 pKa = 11.84HH336 pKa = 5.73QRR338 pKa = 11.84SQGG341 pKa = 3.27

Molecular weight: 36.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2550

0

2550

735677

28

3850

288.5

32.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.419 ± 0.054

0.863 ± 0.013

4.917 ± 0.038

7.593 ± 0.059

4.195 ± 0.04

7.354 ± 0.058

2.328 ± 0.025

6.629 ± 0.046

6.531 ± 0.046

9.982 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.022

3.331 ± 0.029

4.385 ± 0.034

4.072 ± 0.034

5.42 ± 0.047

5.379 ± 0.033

4.807 ± 0.032

7.461 ± 0.039

1.309 ± 0.021

3.191 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski