Thioalkalivibrio sulfidiphilus (strain HL-EbGR7)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Thioalkalivibrio; Thioalkalivibrio sulfidiphilus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3272 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8GM80|B8GM80_THISH Uncharacterized protein OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=Tgr7_2592 PE=4 SV=1
MM1 pKa = 7.58GFPIRR6 pKa = 11.84SLSALALCLGVLLLQSGCGGGGGDD30 pKa = 5.63DD31 pKa = 4.31EE32 pKa = 5.37PPLPSGGWVTITSPTTDD49 pKa = 3.34PSFTDD54 pKa = 3.76YY55 pKa = 11.43CNEE58 pKa = 3.71AYY60 pKa = 10.76LFGEE64 pKa = 4.58AFISPDD70 pKa = 2.67WSAVGFDD77 pKa = 3.61GSVITGVTVTWYY89 pKa = 10.56NAATGQSGPATQSASICYY107 pKa = 9.91ILGTPVPCNHH117 pKa = 5.52VWWATVPMEE126 pKa = 4.38VGFNEE131 pKa = 3.74IRR133 pKa = 11.84ITATDD138 pKa = 3.66PDD140 pKa = 4.44GRR142 pKa = 11.84TGTDD146 pKa = 3.01TLTIDD151 pKa = 3.15HH152 pKa = 7.54PEE154 pKa = 3.9RR155 pKa = 11.84SFSLSGTVKK164 pKa = 9.1TDD166 pKa = 2.98AGIGLSFRR174 pKa = 11.84EE175 pKa = 4.08SQIDD179 pKa = 4.49LILSDD184 pKa = 3.77GSTTRR189 pKa = 11.84RR190 pKa = 11.84TVTSLDD196 pKa = 2.86GSYY199 pKa = 10.34RR200 pKa = 11.84FSCARR205 pKa = 11.84SNMGYY210 pKa = 8.61TITPSSPIPYY220 pKa = 9.72DD221 pKa = 3.45FTPEE225 pKa = 3.94EE226 pKa = 4.06QTVIPQGADD235 pKa = 3.1VSDD238 pKa = 4.73LDD240 pKa = 4.17FSTPAWFLSGLVQLEE255 pKa = 4.31SGGVLSGMMVNVSDD269 pKa = 5.71ASDD272 pKa = 3.37TDD274 pKa = 3.68VTQFTDD280 pKa = 2.94NTGAYY285 pKa = 9.82RR286 pKa = 11.84LALPNGSYY294 pKa = 9.33TVLARR299 pKa = 11.84DD300 pKa = 2.75IWNYY304 pKa = 10.38FSLSPDD310 pKa = 2.62GWTGPVVINGADD322 pKa = 3.32VADD325 pKa = 3.76QNFLATHH332 pKa = 6.79PP333 pKa = 4.15

Molecular weight: 35.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8GRK4|B8GRK4_THISH Glyoxalase/bleomycin resistance protein/dioxygenase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=Tgr7_1474 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.5RR12 pKa = 11.84KK13 pKa = 7.79RR14 pKa = 11.84THH16 pKa = 5.93GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 8.89VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.54GRR39 pKa = 11.84VRR41 pKa = 11.84LCPP44 pKa = 3.58

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3272

0

3272

1002246

31

3954

306.3

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.984 ± 0.057

0.879 ± 0.016

5.703 ± 0.032

6.619 ± 0.045

3.381 ± 0.028

8.285 ± 0.05

2.516 ± 0.021

4.637 ± 0.033

2.672 ± 0.038

11.258 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.535 ± 0.024

2.528 ± 0.022

5.114 ± 0.032

3.699 ± 0.026

8.034 ± 0.044

5.008 ± 0.029

4.814 ± 0.028

7.555 ± 0.035

1.382 ± 0.021

2.4 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski