Ectocarpus sp. CCAP 1310/34

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; PX clade; Phaeophyceae; Ectocarpales; Ectocarpaceae; Ectocarpus; unclassified Ectocarpus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H5JCI3|A0A6H5JCI3_9PHAE Uncharacterized protein (Fragment) OS=Ectocarpus sp. CCAP 1310/34 OX=867726 GN=ESUBFT_1007_7 PE=4 SV=1
MM1 pKa = 7.66SLEE4 pKa = 4.19FRR6 pKa = 11.84LDD8 pKa = 3.2LCGYY12 pKa = 10.14CDD14 pKa = 3.55TYY16 pKa = 11.96NNGSFNPIDD25 pKa = 3.38TFQYY29 pKa = 10.51EE30 pKa = 4.22DD31 pKa = 3.86AEE33 pKa = 4.55GNEE36 pKa = 4.05PNDD39 pKa = 3.7RR40 pKa = 11.84KK41 pKa = 10.61SGYY44 pKa = 7.18QLKK47 pKa = 10.11LDD49 pKa = 3.86CAGIEE54 pKa = 4.08GDD56 pKa = 3.69PVEE59 pKa = 5.34SINATPAPVRR69 pKa = 11.84PPTPSPTAAATPGTSEE85 pKa = 3.95VTIAPSLEE93 pKa = 4.13AATTAAPTGMPAPSSTVSATRR114 pKa = 11.84GPFKK118 pKa = 10.67VTMAPSLEE126 pKa = 4.16ATTAAPTGMEE136 pKa = 3.6TDD138 pKa = 3.18AWLDD142 pKa = 3.49AATSAPSTVATGSPTEE158 pKa = 4.08MVMDD162 pKa = 4.52PWLAAATPAPTMRR175 pKa = 11.84TTAAPTQMATDD186 pKa = 4.57PLLQAATPAPTTQTTAGPTEE206 pKa = 4.57LVTDD210 pKa = 4.65PLLEE214 pKa = 5.03ADD216 pKa = 4.43APVPTTGTTAAPTEE230 pKa = 4.16MAMDD234 pKa = 5.05PSLEE238 pKa = 4.16AATPAPTTQTTAGPTEE254 pKa = 4.57LVTDD258 pKa = 4.65PLLEE262 pKa = 5.03ADD264 pKa = 4.43APVPTTGTTAAPTEE278 pKa = 4.16MAMDD282 pKa = 5.05PSLEE286 pKa = 4.15AATPTPSTVASAAPTKK302 pKa = 10.31MEE304 pKa = 4.5MDD306 pKa = 4.24PSLEE310 pKa = 4.11AATPTPSTVASAAPTKK326 pKa = 10.31MEE328 pKa = 4.5MDD330 pKa = 4.24PSLEE334 pKa = 4.11AATPTPSTVASAAPTKK350 pKa = 10.31MEE352 pKa = 4.5MDD354 pKa = 4.24PSLEE358 pKa = 4.11AATPTPSTVASAAPTEE374 pKa = 4.34MVMDD378 pKa = 5.1PLLGADD384 pKa = 3.91APAPTTATMAPSLEE398 pKa = 4.26AVTASPTEE406 pKa = 3.83MALDD410 pKa = 3.65KK411 pKa = 10.92SLEE414 pKa = 4.43SVTPAPTTQTTAGPTEE430 pKa = 4.57LVTDD434 pKa = 4.52PLLEE438 pKa = 4.98AGAPAPTTGTTAAPTEE454 pKa = 4.16MAMDD458 pKa = 5.05PSLEE462 pKa = 4.15AATPTPSTVASAAPTKK478 pKa = 10.25MVMDD482 pKa = 5.25PSLEE486 pKa = 4.13AATPTPSTVASVAPTEE502 pKa = 4.14MVMDD506 pKa = 5.13PSLEE510 pKa = 4.11AATPTPHH517 pKa = 7.39ADD519 pKa = 3.07TVYY522 pKa = 11.0GSVGRR527 pKa = 11.84TNADD531 pKa = 2.51GHH533 pKa = 6.08GPIAGGSHH541 pKa = 6.52VGTDD545 pKa = 3.61YY546 pKa = 11.58GNHH549 pKa = 6.79GGTDD553 pKa = 3.47TDD555 pKa = 3.35GDD557 pKa = 3.91RR558 pKa = 11.84PIASGSHH565 pKa = 6.88ADD567 pKa = 3.59TDD569 pKa = 4.28HH570 pKa = 6.97ANHH573 pKa = 7.09GGTDD577 pKa = 3.61ADD579 pKa = 3.58SDD581 pKa = 4.35GPITGGSHH589 pKa = 6.66VGTDD593 pKa = 3.61YY594 pKa = 11.57GNHH597 pKa = 6.73GGTHH601 pKa = 6.63ANGDD605 pKa = 3.8GPIASGSHH613 pKa = 6.96ADD615 pKa = 3.08TDD617 pKa = 4.09YY618 pKa = 11.95ANDD621 pKa = 3.21GRR623 pKa = 11.84TDD625 pKa = 3.66RR626 pKa = 11.84LCNGFSDD633 pKa = 3.94YY634 pKa = 11.0HH635 pKa = 7.56YY636 pKa = 11.36LLAWYY641 pKa = 9.31DD642 pKa = 3.58SSTGSWNSSTDD653 pKa = 2.6TDD655 pKa = 4.02YY656 pKa = 12.02ANDD659 pKa = 3.26GRR661 pKa = 11.84TDD663 pKa = 3.66RR664 pKa = 11.84LCNGFSDD671 pKa = 3.94YY672 pKa = 11.0HH673 pKa = 7.56YY674 pKa = 11.36LLAWYY679 pKa = 9.31DD680 pKa = 3.58SSTGSWNSSTDD691 pKa = 2.6TDD693 pKa = 4.02YY694 pKa = 12.02ANDD697 pKa = 3.26GRR699 pKa = 11.84TDD701 pKa = 3.66RR702 pKa = 11.84LCNGFSDD709 pKa = 3.76YY710 pKa = 11.0HH711 pKa = 7.65YY712 pKa = 11.35LLTWYY717 pKa = 9.99DD718 pKa = 3.22SSTGSRR724 pKa = 11.84NSSTDD729 pKa = 2.78PWNSSAVGGG738 pKa = 4.01

Molecular weight: 75.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H5KJ57|A0A6H5KJ57_9PHAE Uncharacterized protein OS=Ectocarpus sp. CCAP 1310/34 OX=867726 GN=ESUBFT_41_3 PE=4 SV=1
MM1 pKa = 7.47SSSSTTVHH9 pKa = 5.82SRR11 pKa = 11.84RR12 pKa = 11.84PLPPPLSLSSSRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84STSSRR31 pKa = 11.84RR32 pKa = 11.84FPPAPLRR39 pKa = 11.84GTWRR43 pKa = 11.84VPSRR47 pKa = 11.84QVV49 pKa = 2.6

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25329

1

25330

11448718

20

11060

452.0

48.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.134 ± 0.033

1.62 ± 0.007

5.512 ± 0.014

6.582 ± 0.018

2.935 ± 0.011

10.056 ± 0.028

2.105 ± 0.007

2.946 ± 0.01

4.35 ± 0.018

7.88 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.007

2.697 ± 0.01

5.486 ± 0.019

3.439 ± 0.012

7.305 ± 0.016

7.844 ± 0.022

5.331 ± 0.014

6.82 ± 0.015

1.299 ± 0.006

1.828 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski