Gloeothece citriformis (strain PCC 7424) (Cyanothece sp. (strain PCC 7424))

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Chroococcales; Aphanothecaceae; Gloeothece; Gloeothece citriformis

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B7KF08|PYRG_GLOC7 CTP synthase OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=pyrG PE=3 SV=1
MM1 pKa = 7.79AVFTVTNLLDD11 pKa = 3.86NGEE14 pKa = 4.12GSLRR18 pKa = 11.84QAIANANSLDD28 pKa = 3.79GADD31 pKa = 3.79TIEE34 pKa = 4.25FASNLSGGTISLTSGEE50 pKa = 4.22LSITDD55 pKa = 3.73SLTLEE60 pKa = 4.5GLGSDD65 pKa = 4.66LLTISGSDD73 pKa = 3.36LSRR76 pKa = 11.84IFNINDD82 pKa = 2.86QTEE85 pKa = 4.66YY86 pKa = 11.24YY87 pKa = 10.12SYY89 pKa = 11.33NSNILDD95 pKa = 3.7VVISGLTLTNGFVSEE110 pKa = 4.21NTQSFNAPAASGGAIYY126 pKa = 10.36NYY128 pKa = 10.43DD129 pKa = 3.85EE130 pKa = 4.65NLTLIDD136 pKa = 3.77TVITGNTAAGDD147 pKa = 3.49PFAYY151 pKa = 10.44YY152 pKa = 10.57EE153 pKa = 4.01NAEE156 pKa = 4.11ALGGGIFNYY165 pKa = 10.35SGTVTVIDD173 pKa = 4.21SVISSNTAQGGTVLLDD189 pKa = 4.46FIRR192 pKa = 11.84EE193 pKa = 4.14GGFVGSGGGIYY204 pKa = 10.3SRR206 pKa = 11.84TGNVTVINSTISDD219 pKa = 3.61NTAKK223 pKa = 10.03TGRR226 pKa = 11.84GGDD229 pKa = 3.51FVFTYY234 pKa = 9.34EE235 pKa = 4.04SHH237 pKa = 6.55GGGIAGGFNSTITITNSTISNNTVVGANTTNIYY270 pKa = 10.36SRR272 pKa = 11.84EE273 pKa = 4.14GGDD276 pKa = 3.33ARR278 pKa = 11.84GGGISGDD285 pKa = 3.24NVIVNNSSITGNTVLGGNGTGSFYY309 pKa = 11.09VYY311 pKa = 10.05YY312 pKa = 9.78GYY314 pKa = 10.91SGGYY318 pKa = 9.86GGDD321 pKa = 3.26ARR323 pKa = 11.84GGGISGNNVTVSNSTISGNIAQGGNGGIGGKK354 pKa = 10.5ANVFRR359 pKa = 11.84GFFYY363 pKa = 9.49GTNGGRR369 pKa = 11.84GGNATGGGISSANLTMSNSTISGNTAQGGNGANGGQGADD408 pKa = 2.74TATINEE414 pKa = 4.06YY415 pKa = 10.4FGYY418 pKa = 9.8SGYY421 pKa = 11.18YY422 pKa = 10.05YY423 pKa = 10.85YY424 pKa = 10.9FAGNGGNGGNGGNSVGGGVAVSNNALIANSTITNNTLDD462 pKa = 3.81SGTGGVGGSGGLGNEE477 pKa = 4.58SLNIPNGIDD486 pKa = 3.54GTDD489 pKa = 3.3GTIGNEE495 pKa = 3.57IGGGLSANGTVTSTIIAGNTDD516 pKa = 3.37NQDD519 pKa = 3.35LAGTFTSGGNNLIGNGDD536 pKa = 3.85GATGFIDD543 pKa = 4.82GVNDD547 pKa = 3.78DD548 pKa = 4.38QVGTTINPIDD558 pKa = 4.47PLLSPLQDD566 pKa = 3.32NGGLTLTHH574 pKa = 6.64TLLPGSPAIDD584 pKa = 3.69AGSNPSTLISDD595 pKa = 3.33QRR597 pKa = 11.84GAPRR601 pKa = 11.84VAGDD605 pKa = 3.63GADD608 pKa = 3.15IGALEE613 pKa = 4.54LADD616 pKa = 3.85EE617 pKa = 4.6TLLGDD622 pKa = 3.49QSSNSFTSGTGNDD635 pKa = 3.69FLEE638 pKa = 5.31GGDD641 pKa = 5.02GNDD644 pKa = 3.98ALNGSSGNDD653 pKa = 3.62GLRR656 pKa = 11.84GGAGSDD662 pKa = 3.39RR663 pKa = 11.84LIGSDD668 pKa = 4.96GNDD671 pKa = 3.57LLIGGLDD678 pKa = 3.52RR679 pKa = 11.84DD680 pKa = 3.69ILAGGEE686 pKa = 4.13GADD689 pKa = 3.29VFRR692 pKa = 11.84FEE694 pKa = 4.71SLADD698 pKa = 3.74SSLLSPDD705 pKa = 4.32RR706 pKa = 11.84IRR708 pKa = 11.84DD709 pKa = 3.73FNQTEE714 pKa = 4.1GDD716 pKa = 3.78RR717 pKa = 11.84FQLLPTNPSTLWNAGDD733 pKa = 3.61VTGDD737 pKa = 3.48NLRR740 pKa = 11.84VALEE744 pKa = 3.66AAYY747 pKa = 10.46ADD749 pKa = 4.38SNQSAGGSQPLGLNEE764 pKa = 3.78AVLFNFGSRR773 pKa = 11.84SYY775 pKa = 11.43LSINNTISGFNPEE788 pKa = 3.49QDD790 pKa = 3.2LVVEE794 pKa = 4.19VSGIVLASGDD804 pKa = 3.82EE805 pKa = 4.22NAGVLSVSDD814 pKa = 3.81YY815 pKa = 11.13FVV817 pKa = 3.0

Molecular weight: 81.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7KHH1|B7KHH1_GLOC7 Extracellular solute-binding protein family 1 OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=PCC7424_2244 PE=4 SV=1
MM1 pKa = 7.29TRR3 pKa = 11.84RR4 pKa = 11.84TLEE7 pKa = 3.71GTSRR11 pKa = 11.84KK12 pKa = 9.03RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84VSGFRR20 pKa = 11.84TRR22 pKa = 11.84MRR24 pKa = 11.84TINGRR29 pKa = 11.84KK30 pKa = 8.8VIQARR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 10.3GRR40 pKa = 11.84HH41 pKa = 5.42KK42 pKa = 10.85LSVSEE47 pKa = 4.34GG48 pKa = 3.44

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5656

0

5656

1754939

29

2997

310.3

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.748 ± 0.035

1.006 ± 0.011

4.856 ± 0.025

6.686 ± 0.036

4.084 ± 0.024

6.422 ± 0.04

1.844 ± 0.016

7.396 ± 0.029

5.47 ± 0.033

11.219 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.804 ± 0.015

4.687 ± 0.029

4.741 ± 0.029

5.476 ± 0.034

4.742 ± 0.025

6.435 ± 0.028

5.586 ± 0.027

6.035 ± 0.028

1.476 ± 0.015

3.287 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski