Hubei tombus-like virus 32

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGJ2|A0A1L3KGJ2_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 32 OX=1923280 PE=4 SV=1
MM1 pKa = 7.56DD2 pKa = 5.27HH3 pKa = 6.58KK4 pKa = 10.27HH5 pKa = 6.27LRR7 pKa = 11.84DD8 pKa = 3.55SSPVVSDD15 pKa = 4.68DD16 pKa = 4.8DD17 pKa = 4.17MEE19 pKa = 5.04VDD21 pKa = 3.85EE22 pKa = 4.87NQPGYY27 pKa = 10.24EE28 pKa = 4.32LGIAAPSNGKK38 pKa = 9.32SCLPSPVGVPMEE50 pKa = 4.24VDD52 pKa = 3.25TFIDD56 pKa = 3.95DD57 pKa = 4.45VEE59 pKa = 4.37EE60 pKa = 4.11CHH62 pKa = 7.09IGVPALLTDD71 pKa = 3.77VADD74 pKa = 4.25TLASSLRR81 pKa = 11.84LVDD84 pKa = 4.48IIIDD88 pKa = 3.62EE89 pKa = 4.44SVRR92 pKa = 11.84RR93 pKa = 11.84AVNKK97 pKa = 9.61WFDD100 pKa = 3.8NNGFIYY106 pKa = 9.41NQYY109 pKa = 9.29WDD111 pKa = 3.56EE112 pKa = 4.27DD113 pKa = 4.16ANFRR117 pKa = 11.84VDD119 pKa = 5.4DD120 pKa = 5.07DD121 pKa = 4.28EE122 pKa = 5.12LLPLARR128 pKa = 11.84MDD130 pKa = 3.65NMMSHH135 pKa = 6.35TFIPPPIVLRR145 pKa = 11.84PRR147 pKa = 11.84GARR150 pKa = 11.84YY151 pKa = 7.8TYY153 pKa = 10.24DD154 pKa = 3.32NGRR157 pKa = 11.84HH158 pKa = 3.72RR159 pKa = 11.84HH160 pKa = 4.86ARR162 pKa = 11.84LLIMSMCNPDD172 pKa = 3.36RR173 pKa = 11.84VLVRR177 pKa = 11.84SQEE180 pKa = 4.09YY181 pKa = 10.07IIEE184 pKa = 4.46KK185 pKa = 10.57NPGPPKK191 pKa = 9.87PRR193 pKa = 11.84QAAPKK198 pKa = 9.18RR199 pKa = 11.84DD200 pKa = 3.22RR201 pKa = 11.84AGPRR205 pKa = 11.84KK206 pKa = 7.69TDD208 pKa = 3.08ANRR211 pKa = 11.84GHH213 pKa = 6.6IAEE216 pKa = 4.8DD217 pKa = 3.27RR218 pKa = 11.84VVLAFRR224 pKa = 11.84LTQVYY229 pKa = 9.31VRR231 pKa = 11.84GRR233 pKa = 11.84DD234 pKa = 3.4TIYY237 pKa = 10.66PDD239 pKa = 2.69IASRR243 pKa = 11.84DD244 pKa = 3.42RR245 pKa = 11.84FNNWVRR251 pKa = 11.84ANQAQIDD258 pKa = 3.95PAEE261 pKa = 4.16MFVCKK266 pKa = 10.23ICGEE270 pKa = 4.11ASLLLCDD277 pKa = 3.52HH278 pKa = 8.06AIRR281 pKa = 11.84EE282 pKa = 4.42AVVPLACPPPVQAPTYY298 pKa = 9.43RR299 pKa = 11.84YY300 pKa = 10.1LGLRR304 pKa = 11.84RR305 pKa = 11.84LWYY308 pKa = 10.44HH309 pKa = 5.21NSKK312 pKa = 10.84FNFGEE317 pKa = 3.87QNARR321 pKa = 11.84DD322 pKa = 3.71VRR324 pKa = 11.84HH325 pKa = 6.35FDD327 pKa = 3.03NSQISDD333 pKa = 3.52VEE335 pKa = 4.47IIPEE339 pKa = 4.34CYY341 pKa = 10.43NYY343 pKa = 8.66ITSNMQTSYY352 pKa = 11.53DD353 pKa = 3.72VAGKK357 pKa = 10.09DD358 pKa = 3.53SRR360 pKa = 11.84EE361 pKa = 3.82LRR363 pKa = 11.84LSHH366 pKa = 6.05CHH368 pKa = 6.12RR369 pKa = 11.84LAGRR373 pKa = 11.84WAEE376 pKa = 3.87LAKK379 pKa = 11.11VKK381 pKa = 10.57DD382 pKa = 3.93RR383 pKa = 11.84LMEE386 pKa = 4.17DD387 pKa = 2.78TWFKK391 pKa = 11.33NRR393 pKa = 11.84LLFTAQRR400 pKa = 11.84ACDD403 pKa = 3.73QVEE406 pKa = 4.05TTMLYY411 pKa = 10.78KK412 pKa = 10.1FTDD415 pKa = 3.41PTTQNFGLARR425 pKa = 11.84QLQEE429 pKa = 3.91FVLFTTGFSALVVVLLVCGVTALEE453 pKa = 4.24FLLLLLQNIALGLSPWGLISLRR475 pKa = 11.84GKK477 pKa = 9.89IAMLACRR484 pKa = 11.84IYY486 pKa = 10.75LSKK489 pKa = 10.56LAHH492 pKa = 6.62DD493 pKa = 4.2TQSVTVRR500 pKa = 11.84VFLLCFTYY508 pKa = 8.76LTCLVDD514 pKa = 4.27ARR516 pKa = 11.84AA517 pKa = 3.79

Molecular weight: 58.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGJ2|A0A1L3KGJ2_9VIRU Uncharacterized protein OS=Hubei tombus-like virus 32 OX=1923280 PE=4 SV=1
MM1 pKa = 7.22QNIPVEE7 pKa = 4.19TSARR11 pKa = 11.84HH12 pKa = 5.13SKK14 pKa = 10.65CNSEE18 pKa = 4.12SFLTVFHH25 pKa = 6.78LLDD28 pKa = 3.86VPSGRR33 pKa = 11.84TGLTAGSPDD42 pKa = 4.42QITLPLLEE50 pKa = 4.66SQTTTVMSNCYY61 pKa = 9.81HH62 pKa = 5.84NQFDD66 pKa = 4.46SFTNRR71 pKa = 11.84YY72 pKa = 8.47LKK74 pKa = 9.23KK75 pKa = 9.31TPILVPRR82 pKa = 11.84NLNYY86 pKa = 10.88GLILRR91 pKa = 11.84IVKK94 pKa = 10.13HH95 pKa = 5.93LVSQVKK101 pKa = 9.01QFYY104 pKa = 10.42KK105 pKa = 10.82PEE107 pKa = 3.93FSFNKK112 pKa = 9.75YY113 pKa = 8.42VQSKK117 pKa = 9.41PGGSRR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 11.84FLRR127 pKa = 11.84AYY129 pKa = 9.81SQLLKK134 pKa = 10.09GTNEE138 pKa = 3.79LPKK141 pKa = 9.98ISKK144 pKa = 8.2ITAFVKK150 pKa = 10.09NEE152 pKa = 3.85RR153 pKa = 11.84YY154 pKa = 9.52FDD156 pKa = 3.7EE157 pKa = 4.74SKK159 pKa = 10.35SPRR162 pKa = 11.84MIMGRR167 pKa = 11.84DD168 pKa = 3.27PRR170 pKa = 11.84FNILYY175 pKa = 9.74ARR177 pKa = 11.84FIARR181 pKa = 11.84IEE183 pKa = 3.96DD184 pKa = 3.37AFFQLEE190 pKa = 4.19QVANACDD197 pKa = 3.78FHH199 pKa = 9.08KK200 pKa = 10.79CGQKK204 pKa = 10.2FKK206 pKa = 11.47NLIGKK211 pKa = 8.52SKK213 pKa = 11.27SMFEE217 pKa = 4.2NDD219 pKa = 2.83MSKK222 pKa = 11.12YY223 pKa = 9.36EE224 pKa = 3.79ASQRR228 pKa = 11.84EE229 pKa = 3.88FALAIEE235 pKa = 4.19FLVYY239 pKa = 10.85AEE241 pKa = 4.41TVGEE245 pKa = 4.33SEE247 pKa = 4.55IDD249 pKa = 3.54DD250 pKa = 4.58LATLFAVKK258 pKa = 9.16MVKK261 pKa = 9.96SGHH264 pKa = 5.31TGEE267 pKa = 4.17GLKK270 pKa = 10.43FYY272 pKa = 10.49FLHH275 pKa = 6.56CRR277 pKa = 11.84GSGDD281 pKa = 3.39MDD283 pKa = 3.5TGLGNGVLNYY293 pKa = 8.39ITTMYY298 pKa = 10.46FKK300 pKa = 10.54IINFCPLNTDD310 pKa = 2.77CHH312 pKa = 6.56MDD314 pKa = 3.52QKK316 pKa = 11.03CCQFDD321 pKa = 3.7AFVLKK326 pKa = 10.87GDD328 pKa = 4.13DD329 pKa = 3.54SYY331 pKa = 11.79GTVPEE336 pKa = 4.37GVDD339 pKa = 3.71FNMVKK344 pKa = 8.61NTYY347 pKa = 7.87EE348 pKa = 3.82WFGFEE353 pKa = 4.24AKK355 pKa = 10.48LIHH358 pKa = 6.83RR359 pKa = 11.84PDD361 pKa = 3.16GRR363 pKa = 11.84LTEE366 pKa = 4.29FCSGNFIRR374 pKa = 11.84VAGGDD379 pKa = 3.56YY380 pKa = 10.85YY381 pKa = 11.48YY382 pKa = 11.19VQNLRR387 pKa = 11.84KK388 pKa = 9.58LVTSLTTCLNPDD400 pKa = 4.34VIRR403 pKa = 11.84EE404 pKa = 4.14GWVAHH409 pKa = 5.92YY410 pKa = 9.93YY411 pKa = 10.45RR412 pKa = 11.84SLGDD416 pKa = 3.57MYY418 pKa = 11.58AVLYY422 pKa = 10.76KK423 pKa = 10.66NIPFYY428 pKa = 11.26EE429 pKa = 5.05DD430 pKa = 2.57IAGFLQTASSKK441 pKa = 10.81LRR443 pKa = 11.84INVNLVQEE451 pKa = 4.68SYY453 pKa = 9.59GHH455 pKa = 6.98AMAFSHH461 pKa = 7.04FNRR464 pKa = 11.84NVEE467 pKa = 4.38TIDD470 pKa = 3.55ARR472 pKa = 11.84SEE474 pKa = 4.26TILDD478 pKa = 3.47ISDD481 pKa = 3.79CSGLTFPEE489 pKa = 3.99LDD491 pKa = 3.17ALRR494 pKa = 11.84TYY496 pKa = 8.01FTSSKK501 pKa = 10.15ICLPPQYY508 pKa = 10.82NRR510 pKa = 11.84RR511 pKa = 11.84CNIRR515 pKa = 11.84ATKK518 pKa = 9.78RR519 pKa = 11.84AWVDD523 pKa = 3.14LDD525 pKa = 3.57EE526 pKa = 6.52DD527 pKa = 3.4IVYY530 pKa = 10.65NINKK534 pKa = 9.92SSLTKK539 pKa = 10.16EE540 pKa = 3.72MKK542 pKa = 10.44GFRR545 pKa = 11.84KK546 pKa = 8.01TLLRR550 pKa = 11.84CLRR553 pKa = 11.84EE554 pKa = 3.86PLEE557 pKa = 4.22TLGQLAQSGTVSS569 pKa = 2.96

Molecular weight: 65.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1086

517

569

543.0

61.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.446 ± 1.068

2.67 ± 0.027

6.538 ± 1.002

5.064 ± 0.304

5.433 ± 0.986

5.341 ± 0.642

2.302 ± 0.153

5.157 ± 0.092

4.788 ± 1.219

10.037 ± 0.433

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.486 ± 0.02

5.157 ± 0.37

4.512 ± 0.79

3.499 ± 0.013

7.182 ± 0.956

6.169 ± 0.821

5.617 ± 0.562

6.63 ± 0.797

0.921 ± 0.312

4.052 ± 0.549
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski