Lipomyces starkeyi NRRL Y-11557

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Lipomycetaceae; Lipomyces; Lipomyces starkeyi

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8136 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3Q572|A0A1E3Q572_LIPST L-ornithine N(5)-monooxygenase OS=Lipomyces starkeyi NRRL Y-11557 OX=675824 GN=LIPSTDRAFT_4483 PE=3 SV=1
MM1 pKa = 6.57MTLLGLGSDD10 pKa = 4.29IFPGLIFLAAAFDD23 pKa = 4.12EE24 pKa = 4.5VDD26 pKa = 4.66DD27 pKa = 4.39LAASIFDD34 pKa = 3.24WSTWIDD40 pKa = 3.42CSIRR44 pKa = 11.84TVRR47 pKa = 11.84APYY50 pKa = 10.0FLLSLYY56 pKa = 10.02EE57 pKa = 4.02SHH59 pKa = 7.27

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3PTU3|A0A1E3PTU3_LIPST SH3 domain-containing protein OS=Lipomyces starkeyi NRRL Y-11557 OX=675824 GN=LIPSTDRAFT_66811 PE=4 SV=1
MM1 pKa = 6.94TTTTTINVCRR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84KK15 pKa = 7.01RR16 pKa = 11.84QRR18 pKa = 11.84KK19 pKa = 8.23RR20 pKa = 11.84KK21 pKa = 9.19RR22 pKa = 11.84KK23 pKa = 9.03RR24 pKa = 11.84KK25 pKa = 9.15RR26 pKa = 11.84KK27 pKa = 9.15RR28 pKa = 11.84KK29 pKa = 9.15RR30 pKa = 11.84KK31 pKa = 9.15RR32 pKa = 11.84KK33 pKa = 9.15RR34 pKa = 11.84KK35 pKa = 9.15RR36 pKa = 11.84KK37 pKa = 9.15RR38 pKa = 11.84KK39 pKa = 9.15RR40 pKa = 11.84KK41 pKa = 9.15RR42 pKa = 11.84KK43 pKa = 9.15RR44 pKa = 11.84KK45 pKa = 9.15RR46 pKa = 11.84KK47 pKa = 9.15RR48 pKa = 11.84KK49 pKa = 9.2RR50 pKa = 11.84KK51 pKa = 9.48RR52 pKa = 11.84KK53 pKa = 9.26

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8135

1

8136

3319957

50

5672

408.1

45.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.952 ± 0.023

1.253 ± 0.012

5.754 ± 0.024

6.139 ± 0.026

4.022 ± 0.019

6.102 ± 0.025

2.212 ± 0.011

5.712 ± 0.02

5.004 ± 0.024

9.153 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.01

3.853 ± 0.012

5.228 ± 0.022

3.807 ± 0.025

5.879 ± 0.023

8.646 ± 0.034

5.9 ± 0.019

6.644 ± 0.02

1.3 ± 0.011

3.198 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski