Human gokushovirus

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9Q0U7|A0A1X9Q0U7_9VIRU Putative VP4 OS=Human gokushovirus OX=1986030 PE=4 SV=1
MM1 pKa = 6.93QRR3 pKa = 11.84PRR5 pKa = 11.84SKK7 pKa = 10.53FDD9 pKa = 3.45RR10 pKa = 11.84GHH12 pKa = 6.02QLLTTINEE20 pKa = 4.36GEE22 pKa = 4.28LVPIYY27 pKa = 9.68MDD29 pKa = 3.41EE30 pKa = 4.19VLPGDD35 pKa = 3.74TARR38 pKa = 11.84VQLNGLIRR46 pKa = 11.84MSTPIYY52 pKa = 9.97PIMDD56 pKa = 3.93NCYY59 pKa = 7.94MDD61 pKa = 3.49TYY63 pKa = 11.04FFFVPSRR70 pKa = 11.84LLWEE74 pKa = 4.4HH75 pKa = 6.43FEE77 pKa = 4.56NMFGEE82 pKa = 4.28NDD84 pKa = 3.07TDD86 pKa = 3.34YY87 pKa = 10.41WAEE90 pKa = 3.83KK91 pKa = 9.71TEE93 pKa = 4.34YY94 pKa = 9.2STPTCTIGGTSGLANGSIGDD114 pKa = 3.93YY115 pKa = 10.49FGLPTEE121 pKa = 4.29VNKK124 pKa = 10.55ALKK127 pKa = 10.63VNALPARR134 pKa = 11.84AYY136 pKa = 10.96AMIYY140 pKa = 10.19NEE142 pKa = 3.96WFRR145 pKa = 11.84DD146 pKa = 3.72EE147 pKa = 4.86NLEE150 pKa = 4.14APLMLGYY157 pKa = 10.7KK158 pKa = 8.98KK159 pKa = 9.19TDD161 pKa = 3.62DD162 pKa = 3.93GGTNADD168 pKa = 3.53ASEE171 pKa = 4.33EE172 pKa = 4.2TSSANAIDD180 pKa = 3.77KK181 pKa = 7.6TTNTNEE187 pKa = 3.62ATLYY191 pKa = 10.77AMKK194 pKa = 9.7PARR197 pKa = 11.84AGKK200 pKa = 8.95FHH202 pKa = 7.99DD203 pKa = 4.67YY204 pKa = 8.27FTSCLPSPMKK214 pKa = 10.71NEE216 pKa = 3.91EE217 pKa = 4.12AVTIPLGGIAPVKK230 pKa = 10.15NYY232 pKa = 10.04KK233 pKa = 10.26HH234 pKa = 6.93EE235 pKa = 4.4DD236 pKa = 3.31LKK238 pKa = 11.39DD239 pKa = 3.52PRR241 pKa = 11.84NVTLYY246 pKa = 10.35AWGAVSEE253 pKa = 4.37PGQPEE258 pKa = 3.88SGYY261 pKa = 11.62KK262 pKa = 9.55MGIQEE267 pKa = 3.95KK268 pKa = 9.83GKK270 pKa = 9.11PVKK273 pKa = 10.31IGFNDD278 pKa = 3.49NNDD281 pKa = 3.38GFLGADD287 pKa = 4.11LNNVAATTINDD298 pKa = 3.3LRR300 pKa = 11.84QAIALQHH307 pKa = 6.14IFEE310 pKa = 5.01ADD312 pKa = 3.02ARR314 pKa = 11.84NGTRR318 pKa = 11.84YY319 pKa = 9.79RR320 pKa = 11.84EE321 pKa = 4.13FLSGTWGVTSPDD333 pKa = 3.02SRR335 pKa = 11.84LQIPEE340 pKa = 4.2YY341 pKa = 10.48IGGQRR346 pKa = 11.84IAINVNQVIQTSQTDD361 pKa = 3.53AQTGQALGNTAAYY374 pKa = 10.84SLTTCSKK381 pKa = 11.1QMVDD385 pKa = 3.55YY386 pKa = 10.91AATEE390 pKa = 3.75YY391 pKa = 11.22GYY393 pKa = 10.2IIGLAVVRR401 pKa = 11.84VEE403 pKa = 4.73HH404 pKa = 7.07SYY406 pKa = 10.5QQGLGTKK413 pKa = 6.63WTRR416 pKa = 11.84GGRR419 pKa = 11.84FTYY422 pKa = 10.38YY423 pKa = 10.69DD424 pKa = 3.6PRR426 pKa = 11.84LAGLGEE432 pKa = 3.88QPVYY436 pKa = 10.71NRR438 pKa = 11.84EE439 pKa = 3.49IFAQGTEE446 pKa = 3.94KK447 pKa = 10.76DD448 pKa = 4.01DD449 pKa = 4.66EE450 pKa = 4.26IFGYY454 pKa = 7.68QEE456 pKa = 3.34CWADD460 pKa = 3.51YY461 pKa = 9.94RR462 pKa = 11.84YY463 pKa = 10.4KK464 pKa = 10.46PSYY467 pKa = 8.48VTGEE471 pKa = 3.66MRR473 pKa = 11.84SNYY476 pKa = 7.86KK477 pKa = 9.78TSLDD481 pKa = 3.19AWHH484 pKa = 6.56YY485 pKa = 11.32ADD487 pKa = 6.34DD488 pKa = 4.62YY489 pKa = 11.22DD490 pKa = 4.75TLPTLSAEE498 pKa = 4.66WIQEE502 pKa = 3.34GRR504 pKa = 11.84EE505 pKa = 4.05NIDD508 pKa = 2.82RR509 pKa = 11.84TIAVTSAVSHH519 pKa = 6.26QFLCDD524 pKa = 3.73FWFNEE529 pKa = 3.32AWFRR533 pKa = 11.84EE534 pKa = 4.03MPIYY538 pKa = 10.09SIPGIEE544 pKa = 4.29RR545 pKa = 11.84II546 pKa = 4.12

Molecular weight: 61.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9Q0V8|A0A1X9Q0V8_9VIRU Peptidase M15 OS=Human gokushovirus OX=1986030 PE=4 SV=1
MM1 pKa = 7.4LNEE4 pKa = 3.91YY5 pKa = 11.08SLFYY9 pKa = 10.58QGAHH13 pKa = 5.51RR14 pKa = 11.84VSAHH18 pKa = 5.57FKK20 pKa = 10.16IKK22 pKa = 10.41EE23 pKa = 3.82FAQKK27 pKa = 10.44DD28 pKa = 4.02GRR30 pKa = 11.84CDD32 pKa = 3.43KK33 pKa = 10.95ILIDD37 pKa = 3.95SEE39 pKa = 4.3LVEE42 pKa = 4.09VLEE45 pKa = 5.04DD46 pKa = 3.77VRR48 pKa = 11.84IHH50 pKa = 6.47FNAPVIITSGYY61 pKa = 7.52RR62 pKa = 11.84TPEE65 pKa = 3.74YY66 pKa = 9.43NAKK69 pKa = 10.09IGGVKK74 pKa = 10.34NSQHH78 pKa = 5.73TKK80 pKa = 8.66GTAADD85 pKa = 3.48IRR87 pKa = 11.84VKK89 pKa = 10.5GVPANKK95 pKa = 8.39VQQYY99 pKa = 10.18LKK101 pKa = 10.16HH102 pKa = 6.51KK103 pKa = 8.32YY104 pKa = 6.95WDD106 pKa = 3.47RR107 pKa = 11.84YY108 pKa = 10.54GIGSYY113 pKa = 9.84WNFTHH118 pKa = 7.7IDD120 pKa = 3.37TRR122 pKa = 11.84EE123 pKa = 3.63KK124 pKa = 9.47KK125 pKa = 10.6ARR127 pKa = 11.84WKK129 pKa = 10.5KK130 pKa = 10.27

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1291

130

546

322.8

36.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.753 ± 1.715

0.93 ± 0.286

5.035 ± 0.621

6.584 ± 0.809

3.253 ± 0.478

7.746 ± 0.462

1.549 ± 0.446

5.267 ± 0.631

7.049 ± 1.986

6.429 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.943 ± 0.532

5.964 ± 0.827

4.105 ± 0.511

4.648 ± 0.408

4.88 ± 0.594

6.274 ± 1.445

6.894 ± 0.899

4.183 ± 0.392

1.936 ± 0.248

5.577 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski