Amino acid dipepetide frequency for Henriciella sp. JN25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.955AlaAla: 14.955 ± 0.149	1.306AlaCys: 1.306 ± 0.032	7.242AlaAsp: 7.242 ± 0.078	7.902AlaGlu: 7.902 ± 0.099	4.71AlaPhe: 4.71 ± 0.076	10.668AlaGly: 10.668 ± 0.1	2.082AlaHis: 2.082 ± 0.047	6.193AlaIle: 6.193 ± 0.077	3.975AlaLys: 3.975 ± 0.082	12.338AlaLeu: 12.338 ± 0.135	3.435AlaMet: 3.435 ± 0.063	2.921AlaAsn: 2.921 ± 0.056	5.452AlaPro: 5.452 ± 0.073	3.594AlaGln: 3.594 ± 0.061	8.066AlaArg: 8.066 ± 0.093	6.98AlaSer: 6.98 ± 0.066	5.174AlaThr: 5.174 ± 0.069	7.5AlaVal: 7.5 ± 0.093	1.604AlaTrp: 1.604 ± 0.042	2.694AlaTyr: 2.694 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.07CysAla: 1.07 ± 0.036	0.112CysCys: 0.112 ± 0.009	0.578CysAsp: 0.578 ± 0.022	0.543CysGlu: 0.543 ± 0.019	0.329CysPhe: 0.329 ± 0.018	0.86CysGly: 0.86 ± 0.028	0.198CysHis: 0.198 ± 0.013	0.373CysIle: 0.373 ± 0.018	0.228CysLys: 0.228 ± 0.014	0.81CysLeu: 0.81 ± 0.028	0.176CysMet: 0.176 ± 0.012	0.24CysAsn: 0.24 ± 0.015	0.463CysPro: 0.463 ± 0.023	0.264CysGln: 0.264 ± 0.016	0.532CysArg: 0.532 ± 0.021	0.5CysSer: 0.5 ± 0.021	0.442CysThr: 0.442 ± 0.019	0.628CysVal: 0.628 ± 0.022	0.109CysTrp: 0.109 ± 0.008	0.195CysTyr: 0.195 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.287AspAla: 7.287 ± 0.092	0.534AspCys: 0.534 ± 0.024	3.844AspAsp: 3.844 ± 0.083	4.563AspGlu: 4.563 ± 0.068	2.558AspPhe: 2.558 ± 0.048	5.911AspGly: 5.911 ± 0.089	1.251AspHis: 1.251 ± 0.037	3.424AspIle: 3.424 ± 0.052	2.027AspLys: 2.027 ± 0.049	6.161AspLeu: 6.161 ± 0.079	1.663AspMet: 1.663 ± 0.04	1.458AspAsn: 1.458 ± 0.042	3.566AspPro: 3.566 ± 0.06	1.874AspGln: 1.874 ± 0.041	4.24AspArg: 4.24 ± 0.059	2.552AspSer: 2.552 ± 0.046	3.191AspThr: 3.191 ± 0.056	4.447AspVal: 4.447 ± 0.059	1.256AspTrp: 1.256 ± 0.035	1.688AspTyr: 1.688 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	8.951GluAla: 8.951 ± 0.115	0.381GluCys: 0.381 ± 0.019	4.097GluAsp: 4.097 ± 0.068	4.158GluGlu: 4.158 ± 0.076	2.001GluPhe: 2.001 ± 0.046	4.904GluGly: 4.904 ± 0.073	1.189GluHis: 1.189 ± 0.036	3.622GluIle: 3.622 ± 0.054	2.733GluLys: 2.733 ± 0.062	5.465GluLeu: 5.465 ± 0.072	1.678GluMet: 1.678 ± 0.039	1.914GluAsn: 1.914 ± 0.048	3.027GluPro: 3.027 ± 0.051	2.33GluGln: 2.33 ± 0.046	4.878GluArg: 4.878 ± 0.079	3.101GluSer: 3.101 ± 0.056	4.656GluThr: 4.656 ± 0.066	3.898GluVal: 3.898 ± 0.061	0.83GluTrp: 0.83 ± 0.029	1.223GluTyr: 1.223 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	4.35PheAla: 4.35 ± 0.067	0.4PheCys: 0.4 ± 0.019	3.01PheAsp: 3.01 ± 0.056	2.799PheGlu: 2.799 ± 0.052	1.469PhePhe: 1.469 ± 0.034	3.712PheGly: 3.712 ± 0.072	0.727PheHis: 0.727 ± 0.026	1.869PheIle: 1.869 ± 0.049	1.076PheLys: 1.076 ± 0.038	3.419PheLeu: 3.419 ± 0.066	0.958PheMet: 0.958 ± 0.031	1.19PheAsn: 1.19 ± 0.038	1.542PhePro: 1.542 ± 0.035	1.046PheGln: 1.046 ± 0.031	2.234PheArg: 2.234 ± 0.038	2.475PheSer: 2.475 ± 0.053	1.982PheThr: 1.982 ± 0.038	2.613PheVal: 2.613 ± 0.053	0.561PheTrp: 0.561 ± 0.024	0.924PheTyr: 0.924 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	9.179GlyAla: 9.179 ± 0.106	0.794GlyCys: 0.794 ± 0.027	5.017GlyAsp: 5.017 ± 0.071	5.723GlyGlu: 5.723 ± 0.076	3.696GlyPhe: 3.696 ± 0.066	7.387GlyGly: 7.387 ± 0.1	1.904GlyHis: 1.904 ± 0.043	4.196GlyIle: 4.196 ± 0.065	3.314GlyLys: 3.314 ± 0.058	8.806GlyLeu: 8.806 ± 0.095	2.334GlyMet: 2.334 ± 0.047	2.194GlyAsn: 2.194 ± 0.044	3.686GlyPro: 3.686 ± 0.063	2.897GlyGln: 2.897 ± 0.059	5.557GlyArg: 5.557 ± 0.067	4.476GlySer: 4.476 ± 0.068	4.231GlyThr: 4.231 ± 0.064	6.154GlyVal: 6.154 ± 0.089	1.514GlyTrp: 1.514 ± 0.039	2.336GlyTyr: 2.336 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.159HisAla: 2.159 ± 0.043	0.213HisCys: 0.213 ± 0.013	1.195HisAsp: 1.195 ± 0.031	1.159HisGlu: 1.159 ± 0.036	0.767HisPhe: 0.767 ± 0.024	1.768HisGly: 1.768 ± 0.048	0.5HisHis: 0.5 ± 0.025	0.967HisIle: 0.967 ± 0.027	0.56HisLys: 0.56 ± 0.023	1.811HisLeu: 1.811 ± 0.046	0.469HisMet: 0.469 ± 0.022	0.464HisAsn: 0.464 ± 0.019	1.207HisPro: 1.207 ± 0.033	0.578HisGln: 0.578 ± 0.024	1.237HisArg: 1.237 ± 0.032	0.9HisSer: 0.9 ± 0.029	0.837HisThr: 0.837 ± 0.027	1.347HisVal: 1.347 ± 0.034	0.364HisTrp: 0.364 ± 0.017	0.572HisTyr: 0.572 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	6.822IleAla: 6.822 ± 0.079	0.526IleCys: 0.526 ± 0.022	3.838IleAsp: 3.838 ± 0.057	3.896IleGlu: 3.896 ± 0.061	1.722IlePhe: 1.722 ± 0.041	4.746IleGly: 4.746 ± 0.077	0.925IleHis: 0.925 ± 0.03	2.538IleIle: 2.538 ± 0.051	1.505IleLys: 1.505 ± 0.037	4.411IleLeu: 4.411 ± 0.063	1.107IleMet: 1.107 ± 0.033	1.514IleAsn: 1.514 ± 0.039	2.276IlePro: 2.276 ± 0.042	1.325IleGln: 1.325 ± 0.036	3.194IleArg: 3.194 ± 0.053	3.136IleSer: 3.136 ± 0.053	2.59IleThr: 2.59 ± 0.049	3.879IleVal: 3.879 ± 0.065	0.676IleTrp: 0.676 ± 0.028	1.175IleTyr: 1.175 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.812LysAla: 4.812 ± 0.078	0.158LysCys: 0.158 ± 0.013	2.027LysAsp: 2.027 ± 0.047	1.841LysGlu: 1.841 ± 0.048	0.981LysPhe: 0.981 ± 0.028	2.732LysGly: 2.732 ± 0.055	0.665LysHis: 0.665 ± 0.024	1.694LysIle: 1.694 ± 0.04	1.555LysLys: 1.555 ± 0.047	3.537LysLeu: 3.537 ± 0.07	0.798LysMet: 0.798 ± 0.026	0.862LysAsn: 0.862 ± 0.026	2.246LysPro: 2.246 ± 0.048	1.13LysGln: 1.13 ± 0.036	2.539LysArg: 2.539 ± 0.048	2.12LysSer: 2.12 ± 0.043	2.192LysThr: 2.192 ± 0.053	2.425LysVal: 2.425 ± 0.057	0.423LysTrp: 0.423 ± 0.02	0.685LysTyr: 0.685 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	12.09LeuAla: 12.09 ± 0.121	0.816LeuCys: 0.816 ± 0.031	6.131LeuAsp: 6.131 ± 0.079	5.989LeuGlu: 5.989 ± 0.078	3.633LeuPhe: 3.633 ± 0.064	7.774LeuGly: 7.774 ± 0.084	1.664LeuHis: 1.664 ± 0.034	5.145LeuIle: 5.145 ± 0.082	4.042LeuLys: 4.042 ± 0.07	8.17LeuLeu: 8.17 ± 0.102	2.287LeuMet: 2.287 ± 0.049	2.812LeuAsn: 2.812 ± 0.046	4.927LeuPro: 4.927 ± 0.069	2.696LeuGln: 2.696 ± 0.05	5.867LeuArg: 5.867 ± 0.082	6.745LeuSer: 6.745 ± 0.094	5.386LeuThr: 5.386 ± 0.072	6.692LeuVal: 6.692 ± 0.082	1.124LeuTrp: 1.124 ± 0.036	2.03LeuTyr: 2.03 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.139MetAla: 3.139 ± 0.057	0.166MetCys: 0.166 ± 0.013	1.387MetAsp: 1.387 ± 0.038	1.322MetGlu: 1.322 ± 0.037	0.759MetPhe: 0.759 ± 0.024	1.874MetGly: 1.874 ± 0.042	0.405MetHis: 0.405 ± 0.02	1.384MetIle: 1.384 ± 0.036	1.203MetLys: 1.203 ± 0.034	2.322MetLeu: 2.322 ± 0.043	0.746MetMet: 0.746 ± 0.032	0.776MetAsn: 0.776 ± 0.026	1.469MetPro: 1.469 ± 0.037	0.842MetGln: 0.842 ± 0.028	1.757MetArg: 1.757 ± 0.038	1.91MetSer: 1.91 ± 0.038	1.867MetThr: 1.867 ± 0.036	1.528MetVal: 1.528 ± 0.04	0.204MetTrp: 0.204 ± 0.014	0.352MetTyr: 0.352 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.132AsnAla: 3.132 ± 0.056	0.222AsnCys: 0.222 ± 0.014	1.557AsnAsp: 1.557 ± 0.044	1.613AsnGlu: 1.613 ± 0.037	1.052AsnPhe: 1.052 ± 0.035	2.514AsnGly: 2.514 ± 0.046	0.49AsnHis: 0.49 ± 0.021	1.34AsnIle: 1.34 ± 0.033	0.769AsnLys: 0.769 ± 0.029	2.535AsnLeu: 2.535 ± 0.049	0.666AsnMet: 0.666 ± 0.023	0.708AsnAsn: 0.708 ± 0.026	1.854AsnPro: 1.854 ± 0.037	0.846AsnGln: 0.846 ± 0.029	1.88AsnArg: 1.88 ± 0.042	1.452AsnSer: 1.452 ± 0.039	1.404AsnThr: 1.404 ± 0.037	1.902AsnVal: 1.902 ± 0.038	0.47AsnTrp: 0.47 ± 0.025	0.728AsnTyr: 0.728 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	5.793ProAla: 5.793 ± 0.077	0.339ProCys: 0.339 ± 0.018	4.296ProAsp: 4.296 ± 0.059	4.073ProGlu: 4.073 ± 0.064	1.96ProPhe: 1.96 ± 0.04	4.622ProGly: 4.622 ± 0.068	0.938ProHis: 0.938 ± 0.026	2.176ProIle: 2.176 ± 0.043	1.785ProLys: 1.785 ± 0.05	4.3ProLeu: 4.3 ± 0.065	1.151ProMet: 1.151 ± 0.029	1.308ProAsn: 1.308 ± 0.037	2.486ProPro: 2.486 ± 0.078	1.571ProGln: 1.571 ± 0.04	2.536ProArg: 2.536 ± 0.046	2.797ProSer: 2.797 ± 0.053	2.115ProThr: 2.115 ± 0.052	4.305ProVal: 4.305 ± 0.058	0.67ProTrp: 0.67 ± 0.026	1.176ProTyr: 1.176 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.006GlnAla: 4.006 ± 0.075	0.232GlnCys: 0.232 ± 0.013	1.672GlnAsp: 1.672 ± 0.043	1.561GlnGlu: 1.561 ± 0.04	1.156GlnPhe: 1.156 ± 0.034	2.309GlnGly: 2.309 ± 0.046	0.599GlnHis: 0.599 ± 0.024	1.676GlnIle: 1.676 ± 0.042	1.147GlnLys: 1.147 ± 0.03	2.987GlnLeu: 2.987 ± 0.055	0.844GlnMet: 0.844 ± 0.03	0.812GlnAsn: 0.812 ± 0.028	1.772GlnPro: 1.772 ± 0.045	1.158GlnGln: 1.158 ± 0.034	2.139GlnArg: 2.139 ± 0.054	1.981GlnSer: 1.981 ± 0.044	1.908GlnThr: 1.908 ± 0.039	2.134GlnVal: 2.134 ± 0.041	0.417GlnTrp: 0.417 ± 0.021	0.616GlnTyr: 0.616 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	7.236ArgAla: 7.236 ± 0.093	0.466ArgCys: 0.466 ± 0.022	3.982ArgAsp: 3.982 ± 0.059	4.304ArgGlu: 4.304 ± 0.069	2.713ArgPhe: 2.713 ± 0.048	4.449ArgGly: 4.449 ± 0.068	1.438ArgHis: 1.438 ± 0.036	3.771ArgIle: 3.771 ± 0.06	2.616ArgLys: 2.616 ± 0.05	7.315ArgLeu: 7.315 ± 0.09	1.768ArgMet: 1.768 ± 0.038	1.826ArgAsn: 1.826 ± 0.041	3.171ArgPro: 3.171 ± 0.052	2.47ArgGln: 2.47 ± 0.047	4.861ArgArg: 4.861 ± 0.086	3.618ArgSer: 3.618 ± 0.069	3.283ArgThr: 3.283 ± 0.049	4.211ArgVal: 4.211 ± 0.062	0.939ArgTrp: 0.939 ± 0.033	1.713ArgTyr: 1.713 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.606SerAla: 6.606 ± 0.087	0.471SerCys: 0.471 ± 0.023	3.77SerAsp: 3.77 ± 0.066	3.613SerGlu: 3.613 ± 0.058	2.361SerPhe: 2.361 ± 0.045	5.925SerGly: 5.925 ± 0.09	1.025SerHis: 1.025 ± 0.036	2.867SerIle: 2.867 ± 0.056	1.888SerLys: 1.888 ± 0.042	5.737SerLeu: 5.737 ± 0.077	1.448SerMet: 1.448 ± 0.04	1.617SerAsn: 1.617 ± 0.044	2.936SerPro: 2.936 ± 0.056	1.776SerGln: 1.776 ± 0.039	3.694SerArg: 3.694 ± 0.054	3.252SerSer: 3.252 ± 0.062	2.69SerThr: 2.69 ± 0.052	4.263SerVal: 4.263 ± 0.067	0.827SerTrp: 0.827 ± 0.027	1.348SerTyr: 1.348 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	5.903ThrAla: 5.903 ± 0.069	0.463ThrCys: 0.463 ± 0.02	3.171ThrAsp: 3.171 ± 0.052	3.008ThrGlu: 3.008 ± 0.046	1.977ThrPhe: 1.977 ± 0.041	5.314ThrGly: 5.314 ± 0.074	0.995ThrHis: 0.995 ± 0.028	2.827ThrIle: 2.827 ± 0.052	1.448ThrLys: 1.448 ± 0.036	5.639ThrLeu: 5.639 ± 0.073	1.152ThrMet: 1.152 ± 0.034	1.37ThrAsn: 1.37 ± 0.033	3.305ThrPro: 3.305 ± 0.052	1.387ThrGln: 1.387 ± 0.038	3.316ThrArg: 3.316 ± 0.06	2.987ThrSer: 2.987 ± 0.049	2.512ThrThr: 2.512 ± 0.05	4.017ThrVal: 4.017 ± 0.067	0.655ThrTrp: 0.655 ± 0.025	1.397ThrTyr: 1.397 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	7.426ValAla: 7.426 ± 0.087	0.671ValCys: 0.671 ± 0.025	4.279ValAsp: 4.279 ± 0.068	4.671ValGlu: 4.671 ± 0.071	2.969ValPhe: 2.969 ± 0.055	4.787ValGly: 4.787 ± 0.069	1.283ValHis: 1.283 ± 0.038	4.024ValIle: 4.024 ± 0.059	2.293ValLys: 2.293 ± 0.046	6.433ValLeu: 6.433 ± 0.085	1.814ValMet: 1.814 ± 0.041	2.004ValAsn: 2.004 ± 0.051	3.326ValPro: 3.326 ± 0.057	1.971ValGln: 1.971 ± 0.044	4.682ValArg: 4.682 ± 0.073	4.845ValSer: 4.845 ± 0.072	4.276ValThr: 4.276 ± 0.065	4.638ValVal: 4.638 ± 0.065	1.003ValTrp: 1.003 ± 0.033	1.62ValTyr: 1.62 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	1.356TrpAla: 1.356 ± 0.039	0.135TrpCys: 0.135 ± 0.011	0.761TrpAsp: 0.761 ± 0.025	0.676TrpGlu: 0.676 ± 0.024	0.573TrpPhe: 0.573 ± 0.026	0.976TrpGly: 0.976 ± 0.03	0.316TrpHis: 0.316 ± 0.017	0.735TrpIle: 0.735 ± 0.024	0.532TrpLys: 0.532 ± 0.02	1.684TrpLeu: 1.684 ± 0.048	0.441TrpMet: 0.441 ± 0.018	0.456TrpAsn: 0.456 ± 0.021	0.678TrpPro: 0.678 ± 0.029	0.566TrpGln: 0.566 ± 0.022	1.177TrpArg: 1.177 ± 0.036	0.935TrpSer: 0.935 ± 0.03	0.894TrpThr: 0.894 ± 0.028	0.837TrpVal: 0.837 ± 0.028	0.23TrpTrp: 0.23 ± 0.013	0.303TrpTyr: 0.303 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.52TyrAla: 2.52 ± 0.047	0.245TyrCys: 0.245 ± 0.014	1.64TyrAsp: 1.64 ± 0.039	1.625TyrGlu: 1.625 ± 0.038	0.972TyrPhe: 0.972 ± 0.03	2.164TyrGly: 2.164 ± 0.044	0.478TyrHis: 0.478 ± 0.019	0.98TyrIle: 0.98 ± 0.027	0.727TyrLys: 0.727 ± 0.023	2.139TyrLeu: 2.139 ± 0.047	0.475TyrMet: 0.475 ± 0.02	0.687TyrAsn: 0.687 ± 0.027	1.007TyrPro: 1.007 ± 0.029	0.804TyrGln: 0.804 ± 0.026	1.763TyrArg: 1.763 ± 0.043	1.352TyrSer: 1.352 ± 0.036	1.213TyrThr: 1.213 ± 0.04	1.597TyrVal: 1.597 ± 0.037	0.388TyrTrp: 0.388 ± 0.018	0.725TyrTyr: 0.725 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3647 proteins (1173922 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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