Amino acid dipepetide frequency for Ramlibacter sp. MAH-25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.599AlaAla: 20.599 ± 0.16
1.434AlaCys: 1.434 ± 0.032
6.835AlaAsp: 6.835 ± 0.063
6.383AlaGlu: 6.383 ± 0.072
4.189AlaPhe: 4.189 ± 0.053
12.934AlaGly: 12.934 ± 0.121
2.632AlaHis: 2.632 ± 0.043
5.091AlaIle: 5.091 ± 0.066
3.297AlaLys: 3.297 ± 0.069
15.072AlaLeu: 15.072 ± 0.111
3.489AlaMet: 3.489 ± 0.05
2.663AlaAsn: 2.663 ± 0.042
7.057AlaPro: 7.057 ± 0.082
5.81AlaGln: 5.81 ± 0.063
10.671AlaArg: 10.671 ± 0.081
6.659AlaSer: 6.659 ± 0.066
6.585AlaThr: 6.585 ± 0.076
9.965AlaVal: 9.965 ± 0.087
2.229AlaTrp: 2.229 ± 0.043
2.387AlaTyr: 2.387 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
1.21CysAla: 1.21 ± 0.028
0.111CysCys: 0.111 ± 0.008
0.516CysAsp: 0.516 ± 0.018
0.504CysGlu: 0.504 ± 0.017
0.273CysPhe: 0.273 ± 0.014
1.006CysGly: 1.006 ± 0.023
0.275CysHis: 0.275 ± 0.013
0.381CysIle: 0.381 ± 0.014
0.2CysLys: 0.2 ± 0.011
0.81CysLeu: 0.81 ± 0.022
0.2CysMet: 0.2 ± 0.011
0.21CysAsn: 0.21 ± 0.012
0.47CysPro: 0.47 ± 0.017
0.231CysGln: 0.231 ± 0.012
0.668CysArg: 0.668 ± 0.022
0.5CysSer: 0.5 ± 0.02
0.481CysThr: 0.481 ± 0.018
0.727CysVal: 0.727 ± 0.02
0.13CysTrp: 0.13 ± 0.008
0.192CysTyr: 0.192 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.975AspAla: 6.975 ± 0.073
0.44AspCys: 0.44 ± 0.018
2.711AspAsp: 2.711 ± 0.05
3.036AspGlu: 3.036 ± 0.046
1.936AspPhe: 1.936 ± 0.041
4.888AspGly: 4.888 ± 0.06
1.153AspHis: 1.153 ± 0.024
2.25AspIle: 2.25 ± 0.036
1.512AspLys: 1.512 ± 0.032
5.784AspLeu: 5.784 ± 0.074
1.099AspMet: 1.099 ± 0.03
1.111AspAsn: 1.111 ± 0.025
3.278AspPro: 3.278 ± 0.046
1.586AspGln: 1.586 ± 0.036
3.814AspArg: 3.814 ± 0.05
2.018AspSer: 2.018 ± 0.037
2.42AspThr: 2.42 ± 0.043
4.272AspVal: 4.272 ± 0.051
0.951AspTrp: 0.951 ± 0.023
1.269AspTyr: 1.269 ± 0.027
0.0AspXaa: 0.0 ± 0.0
Glu
6.878GluAla: 6.878 ± 0.064
0.371GluCys: 0.371 ± 0.014
2.118GluAsp: 2.118 ± 0.045
2.356GluGlu: 2.356 ± 0.042
1.717GluPhe: 1.717 ± 0.038
3.735GluGly: 3.735 ± 0.047
1.307GluHis: 1.307 ± 0.031
2.272GluIle: 2.272 ± 0.043
1.551GluLys: 1.551 ± 0.038
5.846GluLeu: 5.846 ± 0.069
1.2GluMet: 1.2 ± 0.026
1.056GluAsn: 1.056 ± 0.028
2.802GluPro: 2.802 ± 0.041
2.513GluGln: 2.513 ± 0.041
4.84GluArg: 4.84 ± 0.06
2.227GluSer: 2.227 ± 0.04
2.288GluThr: 2.288 ± 0.038
4.162GluVal: 4.162 ± 0.061
0.679GluTrp: 0.679 ± 0.02
0.91GluTyr: 0.91 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
4.246PheAla: 4.246 ± 0.055
0.338PheCys: 0.338 ± 0.014
2.407PheAsp: 2.407 ± 0.039
1.995PheGlu: 1.995 ± 0.036
1.171PhePhe: 1.171 ± 0.033
3.326PheGly: 3.326 ± 0.044
0.794PheHis: 0.794 ± 0.025
1.26PheIle: 1.26 ± 0.03
0.883PheLys: 0.883 ± 0.026
3.021PheLeu: 3.021 ± 0.048
0.67PheMet: 0.67 ± 0.022
0.921PheAsn: 0.921 ± 0.025
1.635PhePro: 1.635 ± 0.035
1.083PheGln: 1.083 ± 0.027
2.091PheArg: 2.091 ± 0.035
1.851PheSer: 1.851 ± 0.032
1.88PheThr: 1.88 ± 0.036
2.661PheVal: 2.661 ± 0.047
0.465PheTrp: 0.465 ± 0.017
0.761PheTyr: 0.761 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
10.395GlyAla: 10.395 ± 0.102
0.961GlyCys: 0.961 ± 0.026
4.023GlyAsp: 4.023 ± 0.061
4.42GlyGlu: 4.42 ± 0.055
3.253GlyPhe: 3.253 ± 0.041
7.95GlyGly: 7.95 ± 0.142
2.04GlyHis: 2.04 ± 0.036
4.009GlyIle: 4.009 ± 0.05
2.992GlyLys: 2.992 ± 0.046
9.237GlyLeu: 9.237 ± 0.083
2.232GlyMet: 2.232 ± 0.036
2.141GlyAsn: 2.141 ± 0.048
3.918GlyPro: 3.918 ± 0.054
3.541GlyGln: 3.541 ± 0.057
6.591GlyArg: 6.591 ± 0.076
4.849GlySer: 4.849 ± 0.074
5.023GlyThr: 5.023 ± 0.081
6.716GlyVal: 6.716 ± 0.078
1.604GlyTrp: 1.604 ± 0.033
2.356GlyTyr: 2.356 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.924HisAla: 2.924 ± 0.046
0.27HisCys: 0.27 ± 0.014
1.218HisAsp: 1.218 ± 0.029
1.202HisGlu: 1.202 ± 0.029
0.814HisPhe: 0.814 ± 0.023
2.243HisGly: 2.243 ± 0.039
0.6HisHis: 0.6 ± 0.021
0.801HisIle: 0.801 ± 0.02
0.484HisLys: 0.484 ± 0.018
2.334HisLeu: 2.334 ± 0.032
0.453HisMet: 0.453 ± 0.016
0.418HisAsn: 0.418 ± 0.017
1.608HisPro: 1.608 ± 0.034
0.703HisGln: 0.703 ± 0.023
1.604HisArg: 1.604 ± 0.033
0.89HisSer: 0.89 ± 0.022
0.959HisThr: 0.959 ± 0.027
1.86HisVal: 1.86 ± 0.034
0.403HisTrp: 0.403 ± 0.019
0.557HisTyr: 0.557 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
5.655IleAla: 5.655 ± 0.057
0.361IleCys: 0.361 ± 0.015
2.723IleAsp: 2.723 ± 0.043
2.624IleGlu: 2.624 ± 0.045
1.023IlePhe: 1.023 ± 0.028
3.996IleGly: 3.996 ± 0.05
0.771IleHis: 0.771 ± 0.023
1.108IleIle: 1.108 ± 0.027
1.109IleLys: 1.109 ± 0.027
3.054IleLeu: 3.054 ± 0.047
0.57IleMet: 0.57 ± 0.02
1.083IleAsn: 1.083 ± 0.03
1.959IlePro: 1.959 ± 0.035
1.144IleGln: 1.144 ± 0.03
2.589IleArg: 2.589 ± 0.04
1.809IleSer: 1.809 ± 0.035
2.141IleThr: 2.141 ± 0.047
3.403IleVal: 3.403 ± 0.052
0.374IleTrp: 0.374 ± 0.012
0.742IleTyr: 0.742 ± 0.018
0.0IleXaa: 0.0 ± 0.0
Lys
3.809LysAla: 3.809 ± 0.063
0.142LysCys: 0.142 ± 0.011
1.534LysAsp: 1.534 ± 0.034
1.291LysGlu: 1.291 ± 0.032
0.799LysPhe: 0.799 ± 0.027
2.158LysGly: 2.158 ± 0.042
0.496LysHis: 0.496 ± 0.018
1.114LysIle: 1.114 ± 0.025
1.075LysLys: 1.075 ± 0.037
3.076LysLeu: 3.076 ± 0.053
0.623LysMet: 0.623 ± 0.022
0.701LysAsn: 0.701 ± 0.023
1.853LysPro: 1.853 ± 0.041
0.985LysGln: 0.985 ± 0.024
1.812LysArg: 1.812 ± 0.038
1.369LysSer: 1.369 ± 0.034
1.474LysThr: 1.474 ± 0.034
2.36LysVal: 2.36 ± 0.038
0.321LysTrp: 0.321 ± 0.014
0.544LysTyr: 0.544 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
16.755LeuAla: 16.755 ± 0.13
0.973LeuCys: 0.973 ± 0.024
5.828LeuAsp: 5.828 ± 0.065
5.289LeuGlu: 5.289 ± 0.073
3.297LeuPhe: 3.297 ± 0.052
9.075LeuGly: 9.075 ± 0.087
2.44LeuHis: 2.44 ± 0.048
3.122LeuIle: 3.122 ± 0.048
2.961LeuLys: 2.961 ± 0.05
11.771LeuLeu: 11.771 ± 0.117
2.165LeuMet: 2.165 ± 0.041
2.275LeuAsn: 2.275 ± 0.037
6.595LeuPro: 6.595 ± 0.065
4.559LeuGln: 4.559 ± 0.05
8.532LeuArg: 8.532 ± 0.084
5.146LeuSer: 5.146 ± 0.064
4.775LeuThr: 4.775 ± 0.065
9.12LeuVal: 9.12 ± 0.087
1.345LeuTrp: 1.345 ± 0.032
1.906LeuTyr: 1.906 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
3.074MetAla: 3.074 ± 0.048
0.145MetCys: 0.145 ± 0.01
1.134MetAsp: 1.134 ± 0.027
0.984MetGlu: 0.984 ± 0.023
0.65MetPhe: 0.65 ± 0.02
1.799MetGly: 1.799 ± 0.031
0.507MetHis: 0.507 ± 0.018
0.712MetIle: 0.712 ± 0.02
0.868MetLys: 0.868 ± 0.022
2.375MetLeu: 2.375 ± 0.048
0.472MetMet: 0.472 ± 0.016
0.703MetAsn: 0.703 ± 0.019
1.487MetPro: 1.487 ± 0.027
0.945MetGln: 0.945 ± 0.024
1.657MetArg: 1.657 ± 0.036
1.282MetSer: 1.282 ± 0.026
1.463MetThr: 1.463 ± 0.029
1.736MetVal: 1.736 ± 0.033
0.188MetTrp: 0.188 ± 0.011
0.316MetTyr: 0.316 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.796AsnAla: 2.796 ± 0.049
0.24AsnCys: 0.24 ± 0.012
1.102AsnAsp: 1.102 ± 0.033
1.056AsnGlu: 1.056 ± 0.026
0.816AsnPhe: 0.816 ± 0.023
2.04AsnGly: 2.04 ± 0.047
0.435AsnHis: 0.435 ± 0.015
1.023AsnIle: 1.023 ± 0.03
0.681AsnLys: 0.681 ± 0.02
2.354AsnLeu: 2.354 ± 0.042
0.468AsnMet: 0.468 ± 0.019
0.628AsnAsn: 0.628 ± 0.024
1.731AsnPro: 1.731 ± 0.035
0.769AsnGln: 0.769 ± 0.024
1.601AsnArg: 1.601 ± 0.033
0.986AsnSer: 0.986 ± 0.028
1.175AsnThr: 1.175 ± 0.037
1.806AsnVal: 1.806 ± 0.032
0.356AsnTrp: 0.356 ± 0.012
0.587AsnTyr: 0.587 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
8.898ProAla: 8.898 ± 0.091
0.412ProCys: 0.412 ± 0.016
3.573ProAsp: 3.573 ± 0.045
3.182ProGlu: 3.182 ± 0.045
1.906ProPhe: 1.906 ± 0.033
5.706ProGly: 5.706 ± 0.062
1.238ProHis: 1.238 ± 0.025
1.858ProIle: 1.858 ± 0.039
1.244ProLys: 1.244 ± 0.029
5.671ProLeu: 5.671 ± 0.057
1.286ProMet: 1.286 ± 0.03
1.255ProAsn: 1.255 ± 0.029
3.312ProPro: 3.312 ± 0.056
2.329ProGln: 2.329 ± 0.04
3.622ProArg: 3.622 ± 0.048
2.826ProSer: 2.826 ± 0.039
2.674ProThr: 2.674 ± 0.046
4.865ProVal: 4.865 ± 0.058
0.837ProTrp: 0.837 ± 0.021
1.269ProTyr: 1.269 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
6.135GlnAla: 6.135 ± 0.075
0.287GlnCys: 0.287 ± 0.013
1.792GlnAsp: 1.792 ± 0.037
1.758GlnGlu: 1.758 ± 0.035
1.211GlnPhe: 1.211 ± 0.026
3.159GlnGly: 3.159 ± 0.044
0.896GlnHis: 0.896 ± 0.022
1.316GlnIle: 1.316 ± 0.027
0.998GlnLys: 0.998 ± 0.028
4.429GlnLeu: 4.429 ± 0.053
0.814GlnMet: 0.814 ± 0.025
0.656GlnAsn: 0.656 ± 0.02
2.586GlnPro: 2.586 ± 0.038
1.914GlnGln: 1.914 ± 0.039
3.437GlnArg: 3.437 ± 0.049
1.658GlnSer: 1.658 ± 0.031
1.488GlnThr: 1.488 ± 0.03
3.451GlnVal: 3.451 ± 0.048
0.642GlnTrp: 0.642 ± 0.017
0.687GlnTyr: 0.687 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
8.912ArgAla: 8.912 ± 0.081
0.638ArgCys: 0.638 ± 0.019
3.84ArgAsp: 3.84 ± 0.052
4.573ArgGlu: 4.573 ± 0.068
2.776ArgPhe: 2.776 ± 0.045
5.271ArgGly: 5.271 ± 0.066
2.045ArgHis: 2.045 ± 0.034
3.462ArgIle: 3.462 ± 0.044
2.053ArgLys: 2.053 ± 0.038
8.901ArgLeu: 8.901 ± 0.097
1.928ArgMet: 1.928 ± 0.032
1.752ArgAsn: 1.752 ± 0.036
4.242ArgPro: 4.242 ± 0.062
3.209ArgGln: 3.209 ± 0.045
6.282ArgArg: 6.282 ± 0.08
3.902ArgSer: 3.902 ± 0.05
3.741ArgThr: 3.741 ± 0.047
5.43ArgVal: 5.43 ± 0.054
1.355ArgTrp: 1.355 ± 0.025
1.782ArgTyr: 1.782 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
5.939SerAla: 5.939 ± 0.064
0.459SerCys: 0.459 ± 0.018
2.326SerAsp: 2.326 ± 0.037
2.1SerGlu: 2.1 ± 0.031
1.861SerPhe: 1.861 ± 0.029
5.072SerGly: 5.072 ± 0.073
1.076SerHis: 1.076 ± 0.024
2.039SerIle: 2.039 ± 0.035
1.225SerLys: 1.225 ± 0.033
5.172SerLeu: 5.172 ± 0.056
1.238SerMet: 1.238 ± 0.027
1.148SerAsn: 1.148 ± 0.024
2.892SerPro: 2.892 ± 0.043
1.679SerGln: 1.679 ± 0.036
3.65SerArg: 3.65 ± 0.048
2.666SerSer: 2.666 ± 0.047
2.735SerThr: 2.735 ± 0.044
3.784SerVal: 3.784 ± 0.053
0.752SerTrp: 0.752 ± 0.02
1.177SerTyr: 1.177 ± 0.026
0.0SerXaa: 0.0 ± 0.0
Thr
6.156ThrAla: 6.156 ± 0.077
0.419ThrCys: 0.419 ± 0.016
2.439ThrAsp: 2.439 ± 0.04
2.18ThrGlu: 2.18 ± 0.035
1.749ThrPhe: 1.749 ± 0.035
4.833ThrGly: 4.833 ± 0.071
1.1ThrHis: 1.1 ± 0.024
2.033ThrIle: 2.033 ± 0.036
1.055ThrLys: 1.055 ± 0.027
5.877ThrLeu: 5.877 ± 0.062
1.109ThrMet: 1.109 ± 0.026
1.054ThrAsn: 1.054 ± 0.035
3.635ThrPro: 3.635 ± 0.05
1.666ThrGln: 1.666 ± 0.031
3.52ThrArg: 3.52 ± 0.046
2.625ThrSer: 2.625 ± 0.045
2.741ThrThr: 2.741 ± 0.052
4.373ThrVal: 4.373 ± 0.06
0.731ThrTrp: 0.731 ± 0.022
0.973ThrTyr: 0.973 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
10.664ValAla: 10.664 ± 0.08
0.676ValCys: 0.676 ± 0.02
4.493ValAsp: 4.493 ± 0.046
4.252ValGlu: 4.252 ± 0.053
2.652ValPhe: 2.652 ± 0.042
6.004ValGly: 6.004 ± 0.068
1.746ValHis: 1.746 ± 0.035
3.11ValIle: 3.11 ± 0.047
2.229ValLys: 2.229 ± 0.042
8.951ValLeu: 8.951 ± 0.081
1.705ValMet: 1.705 ± 0.039
1.973ValAsn: 1.973 ± 0.046
4.91ValPro: 4.91 ± 0.057
3.184ValGln: 3.184 ± 0.053
6.155ValArg: 6.155 ± 0.062
3.862ValSer: 3.862 ± 0.045
4.213ValThr: 4.213 ± 0.057
7.193ValVal: 7.193 ± 0.077
0.921ValTrp: 0.921 ± 0.024
1.503ValTyr: 1.503 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.445TrpAla: 1.445 ± 0.029
0.182TrpCys: 0.182 ± 0.01
0.705TrpAsp: 0.705 ± 0.02
0.562TrpGlu: 0.562 ± 0.021
0.564TrpPhe: 0.564 ± 0.019
1.033TrpGly: 1.033 ± 0.025
0.375TrpHis: 0.375 ± 0.017
0.615TrpIle: 0.615 ± 0.019
0.463TrpLys: 0.463 ± 0.017
2.007TrpLeu: 2.007 ± 0.038
0.394TrpMet: 0.394 ± 0.016
0.404TrpAsn: 0.404 ± 0.017
0.821TrpPro: 0.821 ± 0.025
0.733TrpGln: 0.733 ± 0.021
1.312TrpArg: 1.312 ± 0.034
0.848TrpSer: 0.848 ± 0.023
0.838TrpThr: 0.838 ± 0.025
0.992TrpVal: 0.992 ± 0.026
0.295TrpTrp: 0.295 ± 0.014
0.325TrpTyr: 0.325 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.516TyrAla: 2.516 ± 0.04
0.237TyrCys: 0.237 ± 0.011
1.187TyrAsp: 1.187 ± 0.029
1.08TyrGlu: 1.08 ± 0.024
0.802TyrPhe: 0.802 ± 0.024
2.021TyrGly: 2.021 ± 0.037
0.42TyrHis: 0.42 ± 0.016
0.662TyrIle: 0.662 ± 0.021
0.656TyrLys: 0.656 ± 0.018
2.188TyrLeu: 2.188 ± 0.042
0.381TyrMet: 0.381 ± 0.014
0.496TyrAsn: 0.496 ± 0.016
1.172TyrPro: 1.172 ± 0.028
0.73TyrGln: 0.73 ± 0.02
1.648TyrArg: 1.648 ± 0.031
1.015TyrSer: 1.015 ± 0.024
1.172TyrThr: 1.172 ± 0.028
1.521TyrVal: 1.521 ± 0.029
0.336TyrTrp: 0.336 ± 0.014
0.506TyrTyr: 0.506 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5208 proteins (1681097 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski