Geobacillus sp. (strain WCH70)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus; unclassified Geobacillus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5DA41|C5DA41_GEOSW Uncharacterized protein OS=Geobacillus sp. (strain WCH70) OX=471223 GN=GWCH70_1307 PE=4 SV=1
MM1 pKa = 7.65IIASNIEE8 pKa = 4.06EE9 pKa = 4.48IEE11 pKa = 4.16NAFDD15 pKa = 3.63FTDD18 pKa = 4.37SIITGVKK25 pKa = 9.53CVNHH29 pKa = 6.02LTDD32 pKa = 4.45LSISVDD38 pKa = 4.37YY39 pKa = 11.17YY40 pKa = 10.81WDD42 pKa = 3.48IQDD45 pKa = 4.5GKK47 pKa = 11.04SEE49 pKa = 4.37TIIDD53 pKa = 3.86APP55 pKa = 3.91

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5DA04|C5DA04_GEOSW SpoOM family protein OS=Geobacillus sp. (strain WCH70) OX=471223 GN=GWCH70_1266 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.21RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.3VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3048

0

3048

882799

26

1892

289.6

32.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.552 ± 0.049

0.837 ± 0.014

4.731 ± 0.032

7.68 ± 0.051

4.419 ± 0.036

6.594 ± 0.04

2.339 ± 0.022

7.636 ± 0.042

6.952 ± 0.044

9.709 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.712 ± 0.022

3.748 ± 0.032

3.84 ± 0.03

3.975 ± 0.038

5.021 ± 0.034

5.173 ± 0.027

5.154 ± 0.027

7.201 ± 0.038

1.094 ± 0.018

3.635 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski