Firmicutes bacterium CAG:646

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5QXE0|R5QXE0_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:646 OX=1262995 GN=BN747_02142 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 10.13IKK4 pKa = 10.51YY5 pKa = 9.97LSVLMALGLLSAAAAGCGEE24 pKa = 4.28KK25 pKa = 10.37KK26 pKa = 10.5EE27 pKa = 4.43SFDD30 pKa = 3.78AAAYY34 pKa = 5.83TQAYY38 pKa = 9.56LDD40 pKa = 3.97ARR42 pKa = 11.84IKK44 pKa = 11.24GNTEE48 pKa = 3.58EE49 pKa = 4.11YY50 pKa = 10.76AKK52 pKa = 10.92LSGEE56 pKa = 4.2SPEE59 pKa = 4.9DD60 pKa = 3.14LAALFDD66 pKa = 4.25EE67 pKa = 5.27KK68 pKa = 11.14VQSLVTLSIGEE79 pKa = 4.14NSNEE83 pKa = 4.4PGSQLLPPQLSQDD96 pKa = 3.75YY97 pKa = 9.89AAMWKK102 pKa = 10.33DD103 pKa = 3.57VLQHH107 pKa = 5.33TKK109 pKa = 10.62YY110 pKa = 10.61KK111 pKa = 10.63AVEE114 pKa = 3.94AKK116 pKa = 10.32KK117 pKa = 10.77DD118 pKa = 3.42GDD120 pKa = 4.01SYY122 pKa = 12.03AVTIEE127 pKa = 4.14TEE129 pKa = 4.23YY130 pKa = 10.73MDD132 pKa = 6.03LNTPVNDD139 pKa = 4.12LLTEE143 pKa = 4.16KK144 pKa = 9.93LTEE147 pKa = 4.34YY148 pKa = 10.56YY149 pKa = 10.68AEE151 pKa = 4.59SPDD154 pKa = 3.58AQTNASEE161 pKa = 4.65TYY163 pKa = 9.16LQLMLEE169 pKa = 4.89SYY171 pKa = 10.7QEE173 pKa = 4.21VLQEE177 pKa = 3.89PAYY180 pKa = 9.82EE181 pKa = 4.3EE182 pKa = 4.09PAKK185 pKa = 8.85TTVTLSQDD193 pKa = 3.64DD194 pKa = 4.0KK195 pKa = 11.6KK196 pKa = 11.3VWTVSDD202 pKa = 3.99EE203 pKa = 4.23DD204 pKa = 3.94LQTVQDD210 pKa = 4.18GLFLPALYY218 pKa = 10.56SDD220 pKa = 4.74AEE222 pKa = 4.27AEE224 pKa = 4.47GEE226 pKa = 4.37GASDD230 pKa = 3.9PGSLSTEE237 pKa = 3.84ATPDD241 pKa = 3.01QTYY244 pKa = 10.79PEE246 pKa = 5.49DD247 pKa = 4.34LDD249 pKa = 3.54QTPTHH254 pKa = 6.23TLGEE258 pKa = 4.38SFILQQDD265 pKa = 3.87GQDD268 pKa = 3.18MVEE271 pKa = 4.17FSVDD275 pKa = 3.16KK276 pKa = 11.68VEE278 pKa = 4.35VTDD281 pKa = 4.12EE282 pKa = 3.94RR283 pKa = 11.84SEE285 pKa = 4.26YY286 pKa = 9.82DD287 pKa = 2.93TSNPEE292 pKa = 3.7KK293 pKa = 10.67VVVVTYY299 pKa = 8.57TYY301 pKa = 11.25KK302 pKa = 10.92NLGFADD308 pKa = 5.36PILYY312 pKa = 10.11DD313 pKa = 3.4QMSFKK318 pKa = 10.76LLEE321 pKa = 4.56GEE323 pKa = 4.82STCSPYY329 pKa = 10.95YY330 pKa = 10.27LQSLSPADD338 pKa = 3.43IATKK342 pKa = 10.69GGDD345 pKa = 3.39SVTASLAYY353 pKa = 9.9GVSADD358 pKa = 3.78CKK360 pKa = 10.02EE361 pKa = 3.96VTVYY365 pKa = 11.06VNNSQIEE372 pKa = 4.49SPFQVTVPLAA382 pKa = 3.77

Molecular weight: 41.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5RMH7|R5RMH7_9FIRM Phosphoribosylaminoimidazolesuccinocarboxamide synthase OS=Firmicutes bacterium CAG:646 OX=1262995 GN=BN747_01114 PE=4 SV=1
MM1 pKa = 7.57LPTVVYY7 pKa = 10.17NYY9 pKa = 9.46RR10 pKa = 11.84TMFVQALCQIVNRR23 pKa = 11.84LCEE26 pKa = 4.21VLLHH30 pKa = 7.13LSGLDD35 pKa = 3.33LAYY38 pKa = 10.55APGFIKK44 pKa = 10.41WSPGNNTGMIVILFYY59 pKa = 11.08NFHH62 pKa = 6.87PLSDD66 pKa = 4.1KK67 pKa = 9.7FTHH70 pKa = 6.49CIIRR74 pKa = 11.84ILVSGSHH81 pKa = 6.99LAPHH85 pKa = 5.55QHH87 pKa = 6.76PFYY90 pKa = 9.62ITPIQEE96 pKa = 4.02SFIFNLLMFSQSIVTQSLNLLNIFDD121 pKa = 3.94QRR123 pKa = 11.84LLTGRR128 pKa = 11.84CQMRR132 pKa = 11.84IFPISLIQNQPLINRR147 pKa = 11.84TTVQQHH153 pKa = 5.09IRR155 pKa = 11.84TLNANISHH163 pKa = 6.21GKK165 pKa = 8.11IRR167 pKa = 11.84TYY169 pKa = 10.37RR170 pKa = 11.84VRR172 pKa = 11.84HH173 pKa = 5.83FSLEE177 pKa = 3.78HH178 pKa = 5.99HH179 pKa = 6.88FKK181 pKa = 11.31LNIIQFRR188 pKa = 11.84FFRR191 pKa = 11.84RR192 pKa = 11.84PRR194 pKa = 11.84PYY196 pKa = 11.07APFQPEE202 pKa = 3.79IIIPQEE208 pKa = 4.03YY209 pKa = 9.48IGSRR213 pKa = 11.84LHH215 pKa = 4.88MLRR218 pKa = 11.84RR219 pKa = 11.84NSRR222 pKa = 11.84EE223 pKa = 4.19HH224 pKa = 5.74FRR226 pKa = 11.84HH227 pKa = 6.46RR228 pKa = 11.84YY229 pKa = 6.92PFEE232 pKa = 4.58RR233 pKa = 11.84NRR235 pKa = 11.84CCHH238 pKa = 5.58QKK240 pKa = 9.95IFSHH244 pKa = 5.76DD245 pKa = 3.22TFRR248 pKa = 11.84RR249 pKa = 11.84LHH251 pKa = 7.09LIFQIQSMTFHH262 pKa = 7.11IRR264 pKa = 11.84RR265 pKa = 11.84HH266 pKa = 4.22FHH268 pKa = 5.66TFQMNLGNVLTPHH281 pKa = 7.49RR282 pKa = 11.84FPDD285 pKa = 3.93TGCLHH290 pKa = 6.65IPASKK295 pKa = 10.37ILIDD299 pKa = 3.84PALLSSRR306 pKa = 11.84LLHH309 pKa = 6.61IKK311 pKa = 10.34RR312 pKa = 11.84IFHH315 pKa = 6.63LHH317 pKa = 5.25HH318 pKa = 6.2QFIFSVPNYY327 pKa = 9.12RR328 pKa = 11.84SKK330 pKa = 10.66IKK332 pKa = 10.43RR333 pKa = 11.84KK334 pKa = 9.73SCVTTPVTAYY344 pKa = 9.96ILTIYY349 pKa = 10.32PNLCDD354 pKa = 5.21KK355 pKa = 10.06ITAFKK360 pKa = 9.23MKK362 pKa = 10.19HH363 pKa = 5.9IIFLFQSASIHH374 pKa = 6.21LKK376 pKa = 8.73TSSVPDD382 pKa = 3.57VIMAGFILNPTHH394 pKa = 7.01FCFISKK400 pKa = 8.16WHH402 pKa = 6.42LNGHH406 pKa = 6.27RR407 pKa = 11.84ALKK410 pKa = 10.38ISVPSLLLSTAGIIKK425 pKa = 10.21GKK427 pKa = 9.4IPRR430 pKa = 11.84SIQILPVIPHH440 pKa = 7.41KK441 pKa = 10.56LRR443 pKa = 11.84SWIILSVTLHH453 pKa = 6.42AKK455 pKa = 7.2TLRR458 pKa = 11.84FPIHH462 pKa = 6.41LNSIFVYY469 pKa = 10.59LIGLYY474 pKa = 10.41LIYY477 pKa = 9.86WVCQLNFVNHH487 pKa = 5.94KK488 pKa = 8.47MFSIIYY494 pKa = 7.83TVILSICRR502 pKa = 11.84CKK504 pKa = 9.54QHH506 pKa = 6.47EE507 pKa = 4.41YY508 pKa = 10.73CYY510 pKa = 10.38FPRR513 pKa = 11.84KK514 pKa = 9.03YY515 pKa = 10.38GFMVFPHH522 pKa = 6.88PSPTKK527 pKa = 10.01IRR529 pKa = 11.84IDD531 pKa = 3.64TGSHH535 pKa = 5.65SDD537 pKa = 3.57LFKK540 pKa = 11.0KK541 pKa = 10.51

Molecular weight: 63.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2671

0

2671

834769

29

2951

312.5

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.966 ± 0.05

1.473 ± 0.021

5.194 ± 0.037

8.379 ± 0.064

4.132 ± 0.035

7.245 ± 0.046

1.801 ± 0.021

7.04 ± 0.045

6.93 ± 0.04

9.171 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.171 ± 0.021

4.023 ± 0.033

3.354 ± 0.025

3.737 ± 0.031

4.557 ± 0.041

5.505 ± 0.038

5.236 ± 0.043

6.892 ± 0.039

1.07 ± 0.022

4.121 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski