Candidatus Phytoplasma pruni

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Acholeplasmatales; Acholeplasmataceae; Candidatus Phytoplasma; 16SrIII (X-disease group)

Average proteome isoelectric point is 7.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M1N007|A0A0M1N007_9MOLU Endo-1 4-beta-glucanase-like protein OS=Candidatus Phytoplasma pruni OX=479893 GN=frvX PE=3 SV=1
MM1 pKa = 7.4NEE3 pKa = 4.33KK4 pKa = 10.64KK5 pKa = 10.69NLIDD9 pKa = 4.74PNNSMIDD16 pKa = 3.01ISYY19 pKa = 10.85QILKK23 pKa = 9.58QNKK26 pKa = 7.68TSIPIYY32 pKa = 10.5QLMEE36 pKa = 3.67QVFQIRR42 pKa = 11.84QLDD45 pKa = 3.73FTDD48 pKa = 3.67VEE50 pKa = 4.81KK51 pKa = 11.21VSQLYY56 pKa = 10.75LDD58 pKa = 3.96IVLSGLFVFNGQDD71 pKa = 3.37LWCVKK76 pKa = 10.47AGNLHH81 pKa = 6.49LWDD84 pKa = 3.63KK85 pKa = 10.79EE86 pKa = 4.23YY87 pKa = 11.23YY88 pKa = 10.18AEE90 pKa = 6.08KK91 pKa = 10.21DD92 pKa = 3.69DD93 pKa = 6.34KK94 pKa = 11.47DD95 pKa = 5.1DD96 pKa = 4.94EE97 pKa = 4.37EE98 pKa = 4.52QTDD101 pKa = 3.72IDD103 pKa = 4.35HH104 pKa = 7.36KK105 pKa = 11.18ILDD108 pKa = 4.28FEE110 pKa = 5.68DD111 pKa = 4.04FTLKK115 pKa = 10.55PKK117 pKa = 10.74NDD119 pKa = 3.94DD120 pKa = 3.58SHH122 pKa = 9.05DD123 pKa = 3.87EE124 pKa = 4.5DD125 pKa = 4.41SDD127 pKa = 3.58EE128 pKa = 4.32HH129 pKa = 8.19KK130 pKa = 11.24EE131 pKa = 4.13EE132 pKa = 4.27IEE134 pKa = 3.97EE135 pKa = 4.18ALDD138 pKa = 3.44IDD140 pKa = 5.65SEE142 pKa = 4.67HH143 pKa = 7.31LDD145 pKa = 3.57NDD147 pKa = 3.41IKK149 pKa = 11.04KK150 pKa = 9.29PQVDD154 pKa = 4.49LNSVSDD160 pKa = 4.46DD161 pKa = 3.85DD162 pKa = 5.42VKK164 pKa = 11.32EE165 pKa = 4.31DD166 pKa = 5.58DD167 pKa = 5.04INDD170 pKa = 3.82EE171 pKa = 3.85YY172 pKa = 11.59DD173 pKa = 3.37YY174 pKa = 11.38LYY176 pKa = 11.09DD177 pKa = 3.47QQ178 pKa = 4.9

Molecular weight: 21.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M1MZB5|A0A0M1MZB5_9MOLU Protein RecA OS=Candidatus Phytoplasma pruni OX=479893 GN=recA PE=3 SV=1
MM1 pKa = 7.33FRR3 pKa = 11.84LKK5 pKa = 10.7NQFKK9 pKa = 10.41IIYY12 pKa = 8.77LCLISFILLLFIFNNPLMAMHH33 pKa = 7.05NGNATPNNGHH43 pKa = 6.8HH44 pKa = 5.68NTNFEE49 pKa = 4.25TKK51 pKa = 10.26AHH53 pKa = 6.91ILQEE57 pKa = 4.21MNRR60 pKa = 11.84EE61 pKa = 3.57QAAIVQQIFNARR73 pKa = 11.84NNNDD77 pKa = 3.13SEE79 pKa = 4.65EE80 pKa = 4.36IINNLVRR87 pKa = 11.84QNIQLSQRR95 pKa = 11.84ISTQQINLHH104 pKa = 5.59NAMPHH109 pKa = 5.34EE110 pKa = 4.45NNRR113 pKa = 11.84NQLNNSNNRR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 3.57

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

549

0

549

146841

50

1638

267.5

30.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.405 ± 0.09

0.615 ± 0.027

4.964 ± 0.066

6.879 ± 0.12

5.684 ± 0.117

4.47 ± 0.105

1.946 ± 0.04

9.172 ± 0.112

10.894 ± 0.124

10.639 ± 0.104

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.117 ± 0.045

6.936 ± 0.1

2.943 ± 0.067

4.702 ± 0.093

2.89 ± 0.068

5.783 ± 0.07

4.997 ± 0.07

5.541 ± 0.113

0.604 ± 0.037

3.818 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski