Streptomyces phage ClubPenguin

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bingvirus; unclassified Bingvirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3SW39|A0A6M3SW39_9CAUD Uncharacterized protein OS=Streptomyces phage ClubPenguin OX=2725618 GN=25 PE=4 SV=1
MM1 pKa = 7.12TTLKK5 pKa = 9.81WDD7 pKa = 3.54QPGEE11 pKa = 4.1RR12 pKa = 11.84TYY14 pKa = 10.41EE15 pKa = 3.96TGVSKK20 pKa = 10.91GVLYY24 pKa = 10.33LQNNGIYY31 pKa = 8.91DD32 pKa = 3.6TGVAWNGLTTLTEE45 pKa = 4.32SPSGAEE51 pKa = 4.07SNKK54 pKa = 10.0QYY56 pKa = 11.01ADD58 pKa = 3.02NMVYY62 pKa = 10.87LNLLSVEE69 pKa = 4.19QFGGTIEE76 pKa = 4.49AFTYY80 pKa = 8.75PDD82 pKa = 3.13EE83 pKa = 4.14WAEE86 pKa = 4.21CDD88 pKa = 3.94GSAEE92 pKa = 4.02PTAGVLVGQQPRR104 pKa = 11.84RR105 pKa = 11.84TFGLSYY111 pKa = 8.11QTKK114 pKa = 9.71VGNDD118 pKa = 3.38MDD120 pKa = 4.5PEE122 pKa = 5.55AGYY125 pKa = 10.12KK126 pKa = 9.14IHH128 pKa = 6.88LVYY131 pKa = 10.45GALAAPSEE139 pKa = 4.28KK140 pKa = 10.51AYY142 pKa = 9.79ATVNDD147 pKa = 4.01SPEE150 pKa = 4.04AMGLSWDD157 pKa = 3.4ISTTPVEE164 pKa = 4.58VPGTNPTTGKK174 pKa = 9.4PFKK177 pKa = 9.04PTASLVIDD185 pKa = 3.85STKK188 pKa = 10.52VAASGLTTLTDD199 pKa = 3.63ALYY202 pKa = 8.49GTAGQDD208 pKa = 3.18PRR210 pKa = 11.84LPLPGEE216 pKa = 4.32VISMFSGTVTTVTPTEE232 pKa = 4.01PAFDD236 pKa = 3.68QPTNTITIPTVAGVNYY252 pKa = 10.52YY253 pKa = 9.96IDD255 pKa = 5.51DD256 pKa = 3.94VLQAAGPVVITTDD269 pKa = 3.53TVVTAMPAPGYY280 pKa = 9.93KK281 pKa = 10.1FPAVVDD287 pKa = 3.75NDD289 pKa = 3.43WLYY292 pKa = 9.42THH294 pKa = 6.75VV295 pKa = 4.07

Molecular weight: 31.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SXD8|A0A6M3SXD8_9CAUD Uncharacterized protein OS=Streptomyces phage ClubPenguin OX=2725618 GN=74 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.17KK3 pKa = 9.49RR4 pKa = 11.84ALVSGVVRR12 pKa = 11.84RR13 pKa = 11.84NFAIGMMCGEE23 pKa = 3.69SWYY26 pKa = 10.69RR27 pKa = 11.84LSSWRR32 pKa = 11.84NYY34 pKa = 8.71PLVWKK39 pKa = 10.21LKK41 pKa = 8.79NDD43 pKa = 2.99WGFGIGPILIWSGKK57 pKa = 8.73

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

17748

38

1771

195.0

21.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.336 ± 0.643

0.625 ± 0.12

6.158 ± 0.332

6.446 ± 0.513

3.82 ± 0.19

7.578 ± 0.307

1.831 ± 0.159

5.477 ± 0.258

6.513 ± 0.346

8.001 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.597 ± 0.121

3.989 ± 0.112

3.871 ± 0.236

3.499 ± 0.169

5.037 ± 0.325

6.22 ± 0.34

6.947 ± 0.374

7.28 ± 0.228

1.42 ± 0.147

3.352 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski