Helicobacter bizzozeronii (strain CIII-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; Helicobacter bizzozeronii

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8KQ95|F8KQ95_HELBC Thiamine-monophosphate kinase OS=Helicobacter bizzozeronii (strain CIII-1) OX=1002804 GN=thiL PE=3 SV=1
MM1 pKa = 6.97QVKK4 pKa = 8.19TLDD7 pKa = 4.06PLSQQALEE15 pKa = 4.44EE16 pKa = 4.62IGLDD20 pKa = 3.11WHH22 pKa = 6.89TDD24 pKa = 3.4TDD26 pKa = 3.58NSPYY30 pKa = 10.09ISQDD34 pKa = 3.25LVIVSQSEE42 pKa = 3.36ADD44 pKa = 3.78AYY46 pKa = 11.41YY47 pKa = 9.72EE48 pKa = 4.27ACNEE52 pKa = 3.95LYY54 pKa = 11.13EE55 pKa = 4.45MFVEE59 pKa = 5.0TAQEE63 pKa = 4.11IIEE66 pKa = 4.07HH67 pKa = 6.54DD68 pKa = 3.71RR69 pKa = 11.84FFEE72 pKa = 4.29LDD74 pKa = 3.37IPNSIVPLIKK84 pKa = 10.42QSWEE88 pKa = 4.24NEE90 pKa = 4.54VHH92 pKa = 3.57WHH94 pKa = 5.59IYY96 pKa = 9.97GRR98 pKa = 11.84FDD100 pKa = 4.57LAGGLDD106 pKa = 3.67GKK108 pKa = 9.49PIKK111 pKa = 10.44LLEE114 pKa = 4.39FNADD118 pKa = 3.85TPTMLYY124 pKa = 10.26EE125 pKa = 4.22SAVDD129 pKa = 3.96AMGVTQIQWLL139 pKa = 3.77

Molecular weight: 15.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8KQQ7|F8KQQ7_HELBC Putative mechanosensitive ion channel OS=Helicobacter bizzozeronii (strain CIII-1) OX=1002804 GN=HBZC1_00750 PE=4 SV=1
MM1 pKa = 7.62SIFMAGSPSWRR12 pKa = 11.84VTHH15 pKa = 6.93IPIPRR20 pKa = 11.84LKK22 pKa = 9.94AHH24 pKa = 6.83RR25 pKa = 11.84PIAQRR30 pKa = 11.84RR31 pKa = 11.84AQHH34 pKa = 4.94SHH36 pKa = 6.19ALFF39 pKa = 4.13

Molecular weight: 4.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1959

0

1959

548772

37

2923

280.1

31.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.896 ± 0.053

1.265 ± 0.028

4.749 ± 0.039

5.836 ± 0.065

4.886 ± 0.051

6.529 ± 0.064

2.728 ± 0.031

6.467 ± 0.054

7.1 ± 0.051

11.408 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.025

4.376 ± 0.053

4.15 ± 0.044

4.981 ± 0.056

3.911 ± 0.035

6.294 ± 0.045

4.593 ± 0.045

5.978 ± 0.052

0.879 ± 0.019

3.613 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski