Mycobacterium saskatchewanense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium;

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5294 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X2CET8|A0A1X2CET8_9MYCO TetR family transcriptional regulator OS=Mycobacterium saskatchewanense OX=220927 GN=AWC23_05195 PE=4 SV=1
MM1 pKa = 7.13ITTSGSGPRR10 pKa = 11.84VEE12 pKa = 4.19EE13 pKa = 3.87TSMSFLTAAPEE24 pKa = 4.79AITAAAGNLAHH35 pKa = 7.08IGSSLQEE42 pKa = 3.68ATAAASGQTTTLAAAAADD60 pKa = 4.04EE61 pKa = 4.33VSVAISQLFGTFGEE75 pKa = 4.55DD76 pKa = 3.46FQALSAQAAAFHH88 pKa = 6.27SAFVSVLNGGSAAYY102 pKa = 10.25LSTEE106 pKa = 3.97AASAEE111 pKa = 4.04QVLFHH116 pKa = 7.05AGAAGVPAATLPIIGDD132 pKa = 3.65LGGILGGGTGGTTGGLLGGLGPILSGSPLGPILNGVGQDD171 pKa = 3.43LGGVLSGILGGSPVSLLANPLGPVLQTLSGAFTDD205 pKa = 4.15VPGLQGLGALLQPLLQPVLPALFAPGALPSPAGDD239 pKa = 3.09PWALLFAQTGANLQNLYY256 pKa = 9.1ATWSADD262 pKa = 3.28PFPFLHH268 pKa = 6.56QILVNQQGYY277 pKa = 8.74AQQLGSQMAFALQNFPTTLANAPASLQLGVQSALNFNPVAAAQVFVNQQLGWAGTITTSLQKK339 pKa = 10.79AGADD343 pKa = 3.61LQTTLPVFQADD354 pKa = 3.45MGLASQAIAAGDD366 pKa = 3.55YY367 pKa = 10.51HH368 pKa = 7.23GAVQDD373 pKa = 4.09FTHH376 pKa = 6.76GLLGLFVSGFDD387 pKa = 3.57TSNLSNITVLGPGGDD402 pKa = 4.29LLPILAIPAQQAQAFAGLFPPGSIPGQIAHH432 pKa = 6.81NYY434 pKa = 8.34LNAVSTLTSSTTSTTFGLNLFNPSANVLQADD465 pKa = 4.09AFFGTPLSLAFSVLGAPVSGLNGLATGATVLSAALQSGNGAAAAGALFDD514 pKa = 3.79MPAYY518 pKa = 10.05GLNGFLNGEE527 pKa = 4.51TIVDD531 pKa = 4.24LALPVSTSTVLPSFLGPLGQPLAAILGVAGIDD563 pKa = 3.29PTIPIVIHH571 pKa = 6.35LPFDD575 pKa = 3.88GLLVPPHH582 pKa = 7.06PISSTVDD589 pKa = 3.22VTVGGVLNLPISLTLGGTPFGGLLPLLVNDD619 pKa = 5.65LPRR622 pKa = 11.84QLATAITPP630 pKa = 3.83

Molecular weight: 62.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X2C690|A0A1X2C690_9MYCO Precorrin 6A synthase OS=Mycobacterium saskatchewanense OX=220927 GN=cobF PE=3 SV=1
MM1 pKa = 7.08TALAPTLQAFFTEE14 pKa = 4.29RR15 pKa = 11.84LARR18 pKa = 11.84QKK20 pKa = 10.45NASPHH25 pKa = 4.8TVASYY30 pKa = 11.24RR31 pKa = 11.84DD32 pKa = 3.77TLRR35 pKa = 11.84LLLGFVAEE43 pKa = 4.46RR44 pKa = 11.84TGTPPARR51 pKa = 11.84MQIEE55 pKa = 4.66DD56 pKa = 4.41LDD58 pKa = 4.78APLIGAFLDD67 pKa = 3.29HH68 pKa = 7.52LEE70 pKa = 4.09YY71 pKa = 10.75QRR73 pKa = 11.84ANSVRR78 pKa = 11.84SRR80 pKa = 11.84NTRR83 pKa = 11.84LAAIHH88 pKa = 5.86SLFRR92 pKa = 11.84FAALQHH98 pKa = 6.67PEE100 pKa = 3.76HH101 pKa = 6.86AALIARR107 pKa = 11.84VLAIPPKK114 pKa = 7.9RR115 pKa = 11.84CNRR118 pKa = 11.84AVVSFLNDD126 pKa = 3.25QEE128 pKa = 4.4IDD130 pKa = 3.65ALLAAPDD137 pKa = 3.52TSRR140 pKa = 11.84WIGRR144 pKa = 11.84RR145 pKa = 11.84DD146 pKa = 3.29HH147 pKa = 7.02ALLLVAVQTGLRR159 pKa = 11.84VSEE162 pKa = 4.29LTGLCCGDD170 pKa = 3.35VHH172 pKa = 8.12LGAGAHH178 pKa = 5.1VRR180 pKa = 11.84CVGKK184 pKa = 10.4GRR186 pKa = 11.84KK187 pKa = 7.63QRR189 pKa = 11.84CTPLTAEE196 pKa = 4.33TVAVLRR202 pKa = 11.84GWLAEE207 pKa = 3.86RR208 pKa = 11.84RR209 pKa = 11.84GRR211 pKa = 11.84PEE213 pKa = 5.13DD214 pKa = 3.47PLFPTSRR221 pKa = 11.84GRR223 pKa = 11.84PLSRR227 pKa = 11.84DD228 pKa = 3.18AVALLVTNHH237 pKa = 7.5AITARR242 pKa = 11.84QRR244 pKa = 11.84CPSIDD249 pKa = 3.3AKK251 pKa = 10.48TITPHH256 pKa = 5.29VLRR259 pKa = 11.84HH260 pKa = 4.73STAMTLLRR268 pKa = 11.84AGVDD272 pKa = 3.38TSVIALWLGHH282 pKa = 6.33EE283 pKa = 4.5KK284 pKa = 10.13TDD286 pKa = 3.4TVQIYY291 pKa = 9.11LHH293 pKa = 6.85ADD295 pKa = 3.0LRR297 pKa = 11.84LKK299 pKa = 10.38EE300 pKa = 3.88RR301 pKa = 11.84ALARR305 pKa = 11.84TTPVHH310 pKa = 5.75ATPGRR315 pKa = 11.84YY316 pKa = 8.78RR317 pKa = 11.84PPDD320 pKa = 3.32TLLAFLEE327 pKa = 4.4ALL329 pKa = 3.95

Molecular weight: 36.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5294

0

5294

1722884

27

12626

325.4

34.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.26 ± 0.052

0.832 ± 0.011

6.151 ± 0.031

5.168 ± 0.032

3.058 ± 0.02

9.107 ± 0.039

2.258 ± 0.016

4.118 ± 0.023

2.097 ± 0.02

9.861 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.02 ± 0.014

2.248 ± 0.018

5.988 ± 0.029

2.955 ± 0.019

7.507 ± 0.037

5.37 ± 0.025

5.76 ± 0.023

8.626 ± 0.036

1.474 ± 0.014

2.143 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski