Raineya orbicola

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Raineyaceae; Raineya

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2N3IHX5|A0A2N3IHX5_9BACT Phosphoheptose isomerase OS=Raineya orbicola OX=2016530 GN=gmhA PE=3 SV=1
MM1 pKa = 7.54LSKK4 pKa = 9.64ITYY7 pKa = 9.09KK8 pKa = 10.8KK9 pKa = 10.79LFGFRR14 pKa = 11.84QGTGLTPNILGFGTILLLLYY34 pKa = 8.76WLNVTSSYY42 pKa = 11.21AQAGKK47 pKa = 10.38DD48 pKa = 3.23GALTVTTANTVLNRR62 pKa = 11.84YY63 pKa = 6.59TRR65 pKa = 11.84VTTDD69 pKa = 2.79VTAGSNVITVVNINDD84 pKa = 3.95LNRR87 pKa = 11.84DD88 pKa = 3.78GIGYY92 pKa = 9.57LPAGFVTNSSVYY104 pKa = 10.85ANNALSPGDD113 pKa = 4.61LIIIYY118 pKa = 8.58QAQGAIINSTNTINYY133 pKa = 8.06GQVTNYY139 pKa = 10.27NGAGTYY145 pKa = 9.77EE146 pKa = 4.35FAQVASVSGNNITLACRR163 pKa = 11.84TKK165 pKa = 10.42YY166 pKa = 10.7SYY168 pKa = 10.67FAARR172 pKa = 11.84YY173 pKa = 7.33VQVIRR178 pKa = 11.84VPQYY182 pKa = 7.4TTLTVNAGASVVAVPWGAPSFGGADD207 pKa = 3.43PSALEE212 pKa = 3.86RR213 pKa = 11.84RR214 pKa = 11.84RR215 pKa = 11.84GGFMVVHH222 pKa = 6.65ATNIVNNGTIHH233 pKa = 6.95ANAAGFRR240 pKa = 11.84GGTIDD245 pKa = 4.92PNTSPTGNNFSIAFVTNSSNEE266 pKa = 4.01SAEE269 pKa = 4.03KK270 pKa = 10.61GEE272 pKa = 4.58SIAGYY277 pKa = 10.21RR278 pKa = 11.84DD279 pKa = 4.41DD280 pKa = 5.87YY281 pKa = 12.03DD282 pKa = 3.31NLYY285 pKa = 9.84NGRR288 pKa = 11.84YY289 pKa = 9.28GRR291 pKa = 11.84GAPANGGGGGNGHH304 pKa = 6.34NAGGGGGANGGNPAGWFRR322 pKa = 11.84GAGVMNSFGTCGNPGAWALDD342 pKa = 3.42PDD344 pKa = 4.65YY345 pKa = 10.87IANGNALTNSQGGGRR360 pKa = 11.84GGYY363 pKa = 10.03SFGSSNQNACTLGPSYY379 pKa = 9.82PANFIAPGIPAADD392 pKa = 3.82VQNTAWGGDD401 pKa = 3.23NRR403 pKa = 11.84DD404 pKa = 3.41AVGGLGGRR412 pKa = 11.84PIVSANFQNQIFFGGGGGAGDD433 pKa = 4.25GNNGANADD441 pKa = 3.92GGDD444 pKa = 3.36GGGIVFLIATGNITGSGTISANGEE468 pKa = 3.89NGFNTISGHH477 pKa = 5.7NDD479 pKa = 2.78APGGGGGGGTVAIQATSIANTINIRR504 pKa = 11.84ANGGNGGNQLITNNEE519 pKa = 4.02SEE521 pKa = 4.54GPGGGGGGGVIVINATTDD539 pKa = 3.11GSTKK543 pKa = 10.06QVNGGQNGTTTSAAVTEE560 pKa = 5.09FTANGATSGNSGTISSVSVSLFSIVCTSNLGINKK594 pKa = 9.68AISNSSPNVGTNVTFTLTASNAGPDD619 pKa = 3.44PATNVVVNDD628 pKa = 4.82LLPAGYY634 pKa = 7.96TFVSATPSQGTYY646 pKa = 11.17NNVTGVWNVGSLGIGGSATLTIVATVNASGPYY678 pKa = 9.39TNTATISTPDD688 pKa = 3.28QNDD691 pKa = 3.63PNTSNNTSSVTPTPVPQTNLGVTKK715 pKa = 9.19TASNMTPPVGSNITFTITANNAGPSNATGVVVNDD749 pKa = 3.93VLPSGYY755 pKa = 8.66TFVSATTSQGTYY767 pKa = 10.52NNVSGVWSVGALANGASATLTITVTVNASGSYY799 pKa = 11.11ANTATITGDD808 pKa = 3.19QADD811 pKa = 4.07PVAGNNSSTATPTPVPQTNLGVTKK835 pKa = 9.19TASNMTPPVGSNITFTITANNAGPSNATGVVVNDD869 pKa = 3.93VLPSGYY875 pKa = 8.85TFVSATASQGTYY887 pKa = 10.82NNVSGVWSIGALANGASATLTITVTVNASGSYY919 pKa = 10.16TNTATISGDD928 pKa = 3.31QADD931 pKa = 4.22PVAGNNSSTATPTPVNVAPDD951 pKa = 3.94AVNDD955 pKa = 3.92NYY957 pKa = 11.61TMAQNGNLSNSVATNDD973 pKa = 3.04ILQAGGNPHH982 pKa = 6.88TFTLVSGGSAAANGTLTFNPDD1003 pKa = 2.54GTFTYY1008 pKa = 10.55VPNTGFSGTVSFTYY1022 pKa = 8.95QVCNGVTPINQCDD1035 pKa = 3.48TAVATIVVLPPPVAVNDD1052 pKa = 3.87NAVTPINTPVSGNLSTNDD1070 pKa = 3.55NLPSGGTYY1078 pKa = 9.58TYY1080 pKa = 10.55TIATPPNPAQGTVTINPDD1098 pKa = 2.67GTYY1101 pKa = 10.2TFTPATGFTGTATFTYY1117 pKa = 9.36QVCNQLGQCSTATVSVLVLPPPVAVNDD1144 pKa = 4.08TANTPINTPVNGNASTNDD1162 pKa = 3.48NLPSGGTYY1170 pKa = 9.65TYY1172 pKa = 10.9TVTTPPANGSVTMNPDD1188 pKa = 2.51GTYY1191 pKa = 9.93TYY1193 pKa = 10.29TPNTGFTGTDD1203 pKa = 2.69VFTYY1207 pKa = 7.97QVCNQVGQCSTATVTINVVPAPVANDD1233 pKa = 3.72DD1234 pKa = 4.73SYY1236 pKa = 12.18TMPQNGSLSNSVVGNDD1252 pKa = 3.35VLPASGNPHH1261 pKa = 5.85TFSLVSGGSAAANGTLTFNPDD1282 pKa = 2.54GTFTYY1287 pKa = 10.55VPNTGFSGTVSFTYY1301 pKa = 8.96QVCNSLIPAQCDD1313 pKa = 3.18TAVATIVVLPPPVAVNDD1330 pKa = 3.87NAVTPINTPVSGNLSTNDD1348 pKa = 3.55NLPSGGTYY1356 pKa = 9.58TYY1358 pKa = 10.55TIATPPNPAQGTVTINPDD1376 pKa = 2.67GTYY1379 pKa = 10.2TFTPATGFTGTATFTYY1395 pKa = 9.36QVCNQLGQCSTATVSVLVLPPPVAVNDD1422 pKa = 4.08TANTPINTPVNGNASTNDD1440 pKa = 3.48NLPSGGTYY1448 pKa = 9.65TYY1450 pKa = 10.9TVTTPPANGSVTMNPDD1466 pKa = 2.51GTYY1469 pKa = 9.93TYY1471 pKa = 10.29TPNTGFTGTDD1481 pKa = 2.69VFTYY1485 pKa = 7.97QVCNQVGQCSTATVTINVVPAPVANDD1511 pKa = 3.72DD1512 pKa = 4.73SYY1514 pKa = 12.18TMPQNGSLSNSVVGNDD1530 pKa = 3.35VLPASGNPHH1539 pKa = 5.85TFSLVSGGSAAANGTLTFNPDD1560 pKa = 2.54GTFTYY1565 pKa = 10.55VPNTGFSGTVSFTYY1579 pKa = 8.96QVCNSLIPAQCDD1591 pKa = 3.18TAVATIVVLPPPVAVNDD1608 pKa = 4.1SAVTPINTPVNGNLSSNDD1626 pKa = 3.4NLPAGGTYY1634 pKa = 9.57TYY1636 pKa = 10.64TVATPPNPAQGTVTINPDD1654 pKa = 2.67GTYY1657 pKa = 10.2TFTPATGFTGTASFTYY1673 pKa = 8.85QVCNQLGQCSTATVSVLVLPPPVAVNDD1700 pKa = 4.08TANTPINTPVNGNASTNDD1718 pKa = 3.48NLPSGGTYY1726 pKa = 9.65TYY1728 pKa = 10.9TVTTPPANGSVTMNPDD1744 pKa = 2.51GTYY1747 pKa = 9.93TYY1749 pKa = 10.29TPNTGFTGTDD1759 pKa = 2.69VFTYY1763 pKa = 7.97QVCNQVGQCSTATVTITVPANPVAVNDD1790 pKa = 4.22NYY1792 pKa = 8.04TTPINTPVGGNLAANDD1808 pKa = 4.23LLPTGFTYY1816 pKa = 10.27TFSLVNGGSAASNGTLVVNPDD1837 pKa = 2.74GTYY1840 pKa = 11.12NFTPNTGFSGVVSFTYY1856 pKa = 8.99QVCNQVNVCSTATVTITILAPPVASNDD1883 pKa = 3.79TAGTQPNTPVNGNVSPNDD1901 pKa = 3.43NLPAGGTYY1909 pKa = 9.72TFTVTTPPANGNVTMNPDD1927 pKa = 2.7GSYY1930 pKa = 10.06TYY1932 pKa = 9.88TPNPGFVGTDD1942 pKa = 2.82TFTYY1946 pKa = 8.07TVCNQLGQCSTATVTITVGQPPLAVNDD1973 pKa = 4.25EE1974 pKa = 4.58YY1975 pKa = 11.41NTGAGIPVSGNVGTNDD1991 pKa = 3.43QNPSGGTTTFNLVSGGTATQNGTLVFNPNGIFTFTPNPGFSGTVTFTYY2039 pKa = 9.0EE2040 pKa = 3.02ICNPVGCSQATVTINVAPPVQAVDD2064 pKa = 4.22DD2065 pKa = 4.82NYY2067 pKa = 8.9TTTPNNPVSGNLGTNDD2083 pKa = 3.5VVPAGQAPTFSLVNGGTATQNGTLVVNPDD2112 pKa = 2.9GSFTFTPNPGFVGTVTFTYY2131 pKa = 9.49QVCNQFGQCSTATVTIVIEE2150 pKa = 4.41DD2151 pKa = 3.92PVVQIPEE2158 pKa = 4.3GFSPNNDD2165 pKa = 3.8GINDD2169 pKa = 3.44NFVIKK2174 pKa = 10.24GRR2176 pKa = 11.84NGRR2179 pKa = 11.84PVVLRR2184 pKa = 11.84IYY2186 pKa = 10.41NRR2188 pKa = 11.84WGNLVYY2194 pKa = 10.91EE2195 pKa = 4.34NLNYY2199 pKa = 9.92QDD2201 pKa = 4.39DD2202 pKa = 4.2WNGTSNIGIRR2212 pKa = 11.84IGEE2215 pKa = 4.1QLPDD2219 pKa = 3.07GTYY2222 pKa = 10.3FYY2224 pKa = 10.29TAEE2227 pKa = 4.21FKK2229 pKa = 10.36DD2230 pKa = 3.16TGEE2233 pKa = 3.86RR2234 pKa = 11.84YY2235 pKa = 9.83ARR2237 pKa = 11.84YY2238 pKa = 8.43LTIQRR2243 pKa = 4.08

Molecular weight:
227.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2N3I946|A0A2N3I946_9BACT Outer membrane protein beta-barrel domain OS=Raineya orbicola OX=2016530 GN=Rain11_2252 PE=4 SV=1
MM1 pKa = 7.71IYY3 pKa = 10.38SLHH6 pKa = 6.37KK7 pKa = 10.15RR8 pKa = 11.84GASRR12 pKa = 11.84IVRR15 pKa = 11.84PFLVANYY22 pKa = 10.01YY23 pKa = 9.03NVKK26 pKa = 10.83GNFATFNWLLLVLALLILFASSVAVGSVSIPFTEE60 pKa = 4.04IVKK63 pKa = 9.06TLSGLQEE70 pKa = 3.65NATWKK75 pKa = 10.4FIVWEE80 pKa = 3.99LRR82 pKa = 11.84LPKK85 pKa = 10.41ALTAVLVGVALSVAGLVMQTFFANPLASPSEE116 pKa = 4.25LGVSAGASLGVASITLSSGISWSAFQQWGLVGNSFVAIIAILGAMLVMLLLLLMSRR172 pKa = 11.84FVWNNTTLLIVGLMLGSLAISLIGLWQFLSSPEE205 pKa = 4.0QIRR208 pKa = 11.84DD209 pKa = 3.74FLWWSFGSLAGTNYY223 pKa = 10.5SQIAVLAFLVAMSLLLLFLQTQKK246 pKa = 11.34LNALLLGEE254 pKa = 4.48MYY256 pKa = 10.61ARR258 pKa = 11.84SMGVNIRR265 pKa = 11.84KK266 pKa = 8.91LRR268 pKa = 11.84WVLIVVVSVLSGVTTAFCGPIGFVGLAVPHH298 pKa = 6.8LARR301 pKa = 11.84SLFQTANHH309 pKa = 5.64WTLFPASTLLGASVLLFCDD328 pKa = 3.2ILAYY332 pKa = 10.99NLFANFALPINLVTSLLGSPIVIWIVIRR360 pKa = 11.84RR361 pKa = 11.84SFRR364 pKa = 11.84FF365 pKa = 3.22

Molecular weight:
39.69 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2684

0

2684

933702

29

2470

347.9

39.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.918 ± 0.044

0.854 ± 0.014

4.418 ± 0.027

7.171 ± 0.063

5.538 ± 0.04

5.914 ± 0.051

1.727 ± 0.021

7.718 ± 0.046

7.903 ± 0.064

9.86 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.057 ± 0.019

5.588 ± 0.046

3.644 ± 0.031

4.618 ± 0.031

3.968 ± 0.031

5.655 ± 0.027

5.249 ± 0.048

5.915 ± 0.041

1.229 ± 0.019

4.057 ± 0.028

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski