Cyanobium gracile (strain ATCC 27147 / PCC 6307)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Cyanobium; Cyanobium gracile

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3261 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9PC76|K9PC76_CYAGP Guanylate kinase OS=Cyanobium gracile (strain ATCC 27147 / PCC 6307) OX=292564 GN=gmk PE=3 SV=1
MM1 pKa = 7.85ADD3 pKa = 3.93ALQSSDD9 pKa = 2.67GSRR12 pKa = 11.84PCRR15 pKa = 11.84GLRR18 pKa = 11.84AGSLAGLLLLVLAGGGGRR36 pKa = 11.84VLANVQFEE44 pKa = 4.71NCVTAADD51 pKa = 4.52GSITCDD57 pKa = 3.15TVPTGNTLMNDD68 pKa = 2.34VDD70 pKa = 3.7ARR72 pKa = 11.84YY73 pKa = 10.11GLLQNASPGWSEE85 pKa = 3.63FEE87 pKa = 4.3PYY89 pKa = 9.96EE90 pKa = 5.34GYY92 pKa = 10.94DD93 pKa = 3.61DD94 pKa = 5.91DD95 pKa = 5.75FGGNEE100 pKa = 3.93TT101 pKa = 4.43

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9P532|K9P532_CYAGP Outer membrane protein OS=Cyanobium gracile (strain ATCC 27147 / PCC 6307) OX=292564 GN=Cyagr_0921 PE=3 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.03RR4 pKa = 11.84TLEE7 pKa = 3.92GTSRR11 pKa = 11.84KK12 pKa = 9.24RR13 pKa = 11.84KK14 pKa = 7.95RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84THH26 pKa = 5.95TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84TRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.5

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3261

0

3261

987198

29

4028

302.7

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.162 ± 0.07

1.1 ± 0.016

4.983 ± 0.029

5.658 ± 0.048

3.087 ± 0.027

9.094 ± 0.054

2.07 ± 0.025

3.808 ± 0.035

1.896 ± 0.036

12.951 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.787 ± 0.021

2.055 ± 0.038

6.442 ± 0.044

3.955 ± 0.034

8.139 ± 0.058

5.602 ± 0.041

4.656 ± 0.055

7.07 ± 0.038

1.708 ± 0.021

1.778 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski