Amino acid dipepetide frequency for Kribbella sp. VKM Ac-2527

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.568AlaAla: 18.568 ± 0.124
0.915AlaCys: 0.915 ± 0.019
8.146AlaAsp: 8.146 ± 0.069
7.926AlaGlu: 7.926 ± 0.064
3.525AlaPhe: 3.525 ± 0.043
12.141AlaGly: 12.141 ± 0.079
2.196AlaHis: 2.196 ± 0.029
5.076AlaIle: 5.076 ± 0.051
3.239AlaLys: 3.239 ± 0.039
12.461AlaLeu: 12.461 ± 0.079
2.459AlaMet: 2.459 ± 0.032
2.56AlaAsn: 2.56 ± 0.033
5.446AlaPro: 5.446 ± 0.058
3.636AlaGln: 3.636 ± 0.039
8.296AlaArg: 8.296 ± 0.066
5.941AlaSer: 5.941 ± 0.046
7.388AlaThr: 7.388 ± 0.059
11.128AlaVal: 11.128 ± 0.075
1.824AlaTrp: 1.824 ± 0.027
2.601AlaTyr: 2.601 ± 0.029
0.0AlaXaa: 0.0 ± 0.0
Cys
0.812CysAla: 0.812 ± 0.018
0.109CysCys: 0.109 ± 0.006
0.444CysAsp: 0.444 ± 0.013
0.387CysGlu: 0.387 ± 0.012
0.214CysPhe: 0.214 ± 0.008
0.855CysGly: 0.855 ± 0.02
0.181CysHis: 0.181 ± 0.008
0.207CysIle: 0.207 ± 0.009
0.133CysLys: 0.133 ± 0.007
0.659CysLeu: 0.659 ± 0.014
0.116CysMet: 0.116 ± 0.006
0.162CysAsn: 0.162 ± 0.009
0.428CysPro: 0.428 ± 0.013
0.183CysGln: 0.183 ± 0.008
0.533CysArg: 0.533 ± 0.016
0.445CysSer: 0.445 ± 0.011
0.461CysThr: 0.461 ± 0.014
0.564CysVal: 0.564 ± 0.015
0.131CysTrp: 0.131 ± 0.007
0.148CysTyr: 0.148 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.033AspAla: 7.033 ± 0.059
0.39AspCys: 0.39 ± 0.013
3.911AspAsp: 3.911 ± 0.037
4.091AspGlu: 4.091 ± 0.041
1.663AspPhe: 1.663 ± 0.024
6.15AspGly: 6.15 ± 0.057
1.401AspHis: 1.401 ± 0.023
1.937AspIle: 1.937 ± 0.03
1.34AspLys: 1.34 ± 0.022
6.852AspLeu: 6.852 ± 0.051
0.702AspMet: 0.702 ± 0.014
1.239AspAsn: 1.239 ± 0.022
4.507AspPro: 4.507 ± 0.044
2.164AspGln: 2.164 ± 0.029
4.729AspArg: 4.729 ± 0.045
2.749AspSer: 2.749 ± 0.028
2.896AspThr: 2.896 ± 0.035
5.105AspVal: 5.105 ± 0.045
1.05AspTrp: 1.05 ± 0.02
1.261AspTyr: 1.261 ± 0.02
0.0AspXaa: 0.0 ± 0.0
Glu
6.361GluAla: 6.361 ± 0.061
0.298GluCys: 0.298 ± 0.008
2.561GluAsp: 2.561 ± 0.032
2.748GluGlu: 2.748 ± 0.035
1.662GluPhe: 1.662 ± 0.024
3.476GluGly: 3.476 ± 0.034
1.453GluHis: 1.453 ± 0.024
2.541GluIle: 2.541 ± 0.03
1.292GluLys: 1.292 ± 0.022
7.145GluLeu: 7.145 ± 0.064
0.869GluMet: 0.869 ± 0.019
1.088GluAsn: 1.088 ± 0.019
3.273GluPro: 3.273 ± 0.037
2.392GluGln: 2.392 ± 0.036
4.526GluArg: 4.526 ± 0.043
2.805GluSer: 2.805 ± 0.029
2.863GluThr: 2.863 ± 0.035
4.721GluVal: 4.721 ± 0.039
0.874GluTrp: 0.874 ± 0.017
1.21GluTyr: 1.21 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.847PheAla: 3.847 ± 0.039
0.282PheCys: 0.282 ± 0.01
2.197PheAsp: 2.197 ± 0.026
1.636PheGlu: 1.636 ± 0.024
0.976PhePhe: 0.976 ± 0.021
3.211PheGly: 3.211 ± 0.033
0.643PheHis: 0.643 ± 0.017
0.929PheIle: 0.929 ± 0.022
0.614PheLys: 0.614 ± 0.016
2.666PheLeu: 2.666 ± 0.039
0.412PheMet: 0.412 ± 0.012
0.699PheAsn: 0.699 ± 0.016
1.382PhePro: 1.382 ± 0.024
0.794PheGln: 0.794 ± 0.016
1.892PheArg: 1.892 ± 0.027
1.609PheSer: 1.609 ± 0.027
2.12PheThr: 2.12 ± 0.029
2.657PheVal: 2.657 ± 0.035
0.495PheTrp: 0.495 ± 0.012
0.693PheTyr: 0.693 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
9.134GlyAla: 9.134 ± 0.064
0.802GlyCys: 0.802 ± 0.016
5.013GlyAsp: 5.013 ± 0.045
4.538GlyGlu: 4.538 ± 0.039
3.087GlyPhe: 3.087 ± 0.036
7.783GlyGly: 7.783 ± 0.074
1.907GlyHis: 1.907 ± 0.029
3.956GlyIle: 3.956 ± 0.037
2.615GlyLys: 2.615 ± 0.033
9.417GlyLeu: 9.417 ± 0.066
1.817GlyMet: 1.817 ± 0.028
2.148GlyAsn: 2.148 ± 0.029
4.495GlyPro: 4.495 ± 0.043
2.951GlyGln: 2.951 ± 0.043
6.609GlyArg: 6.609 ± 0.048
5.502GlySer: 5.502 ± 0.051
5.856GlyThr: 5.856 ± 0.058
7.541GlyVal: 7.541 ± 0.06
1.882GlyTrp: 1.882 ± 0.025
2.382GlyTyr: 2.382 ± 0.032
0.0GlyXaa: 0.0 ± 0.0
His
2.216HisAla: 2.216 ± 0.029
0.186HisCys: 0.186 ± 0.008
1.258HisAsp: 1.258 ± 0.023
1.141HisGlu: 1.141 ± 0.02
0.596HisPhe: 0.596 ± 0.014
1.982HisGly: 1.982 ± 0.028
0.632HisHis: 0.632 ± 0.017
0.633HisIle: 0.633 ± 0.015
0.378HisLys: 0.378 ± 0.01
2.286HisLeu: 2.286 ± 0.032
0.265HisMet: 0.265 ± 0.01
0.45HisAsn: 0.45 ± 0.014
1.589HisPro: 1.589 ± 0.023
0.726HisGln: 0.726 ± 0.018
1.832HisArg: 1.832 ± 0.027
0.986HisSer: 0.986 ± 0.019
1.111HisThr: 1.111 ± 0.019
1.629HisVal: 1.629 ± 0.024
0.375HisTrp: 0.375 ± 0.011
0.453HisTyr: 0.453 ± 0.013
0.0HisXaa: 0.0 ± 0.0
Ile
5.764IleAla: 5.764 ± 0.045
0.331IleCys: 0.331 ± 0.013
2.901IleAsp: 2.901 ± 0.032
2.445IleGlu: 2.445 ± 0.032
1.029IlePhe: 1.029 ± 0.021
4.263IleGly: 4.263 ± 0.048
0.735IleHis: 0.735 ± 0.017
1.199IleIle: 1.199 ± 0.023
0.87IleLys: 0.87 ± 0.02
3.027IleLeu: 3.027 ± 0.037
0.507IleMet: 0.507 ± 0.014
0.918IleAsn: 0.918 ± 0.017
2.2IlePro: 2.2 ± 0.026
1.006IleGln: 1.006 ± 0.017
2.783IleArg: 2.783 ± 0.033
2.192IleSer: 2.192 ± 0.031
2.802IleThr: 2.802 ± 0.032
3.48IleVal: 3.48 ± 0.037
0.525IleTrp: 0.525 ± 0.012
0.789IleTyr: 0.789 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
3.1LysAla: 3.1 ± 0.038
0.111LysCys: 0.111 ± 0.007
1.276LysAsp: 1.276 ± 0.025
1.073LysGlu: 1.073 ± 0.022
0.62LysPhe: 0.62 ± 0.017
1.658LysGly: 1.658 ± 0.028
0.518LysHis: 0.518 ± 0.014
0.998LysIle: 0.998 ± 0.021
0.767LysLys: 0.767 ± 0.023
2.534LysLeu: 2.534 ± 0.03
0.398LysMet: 0.398 ± 0.013
0.591LysAsn: 0.591 ± 0.016
1.702LysPro: 1.702 ± 0.031
0.852LysGln: 0.852 ± 0.02
1.498LysArg: 1.498 ± 0.026
1.303LysSer: 1.303 ± 0.022
1.547LysThr: 1.547 ± 0.028
2.258LysVal: 2.258 ± 0.03
0.332LysTrp: 0.332 ± 0.013
0.598LysTyr: 0.598 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
14.397LeuAla: 14.397 ± 0.081
0.697LeuCys: 0.697 ± 0.017
6.767LeuAsp: 6.767 ± 0.051
4.928LeuGlu: 4.928 ± 0.049
2.708LeuPhe: 2.708 ± 0.038
9.089LeuGly: 9.089 ± 0.061
2.071LeuHis: 2.071 ± 0.028
4.184LeuIle: 4.184 ± 0.043
2.228LeuLys: 2.228 ± 0.034
10.721LeuLeu: 10.721 ± 0.08
1.659LeuMet: 1.659 ± 0.025
2.04LeuAsn: 2.04 ± 0.028
6.006LeuPro: 6.006 ± 0.051
2.749LeuGln: 2.749 ± 0.031
7.825LeuArg: 7.825 ± 0.06
5.593LeuSer: 5.593 ± 0.046
7.099LeuThr: 7.099 ± 0.048
9.256LeuVal: 9.256 ± 0.063
1.32LeuTrp: 1.32 ± 0.023
1.879LeuTyr: 1.879 ± 0.026
0.0LeuXaa: 0.0 ± 0.0
Met
2.08MetAla: 2.08 ± 0.031
0.107MetCys: 0.107 ± 0.006
0.831MetAsp: 0.831 ± 0.021
0.688MetGlu: 0.688 ± 0.017
0.537MetPhe: 0.537 ± 0.015
1.172MetGly: 1.172 ± 0.021
0.343MetHis: 0.343 ± 0.012
0.792MetIle: 0.792 ± 0.016
0.48MetLys: 0.48 ± 0.013
1.817MetLeu: 1.817 ± 0.027
0.309MetMet: 0.309 ± 0.011
0.474MetAsn: 0.474 ± 0.014
1.08MetPro: 1.08 ± 0.019
0.458MetGln: 0.458 ± 0.013
1.346MetArg: 1.346 ± 0.021
1.378MetSer: 1.378 ± 0.02
1.608MetThr: 1.608 ± 0.022
1.437MetVal: 1.437 ± 0.022
0.218MetTrp: 0.218 ± 0.008
0.336MetTyr: 0.336 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.508AsnAla: 2.508 ± 0.03
0.184AsnCys: 0.184 ± 0.009
1.213AsnAsp: 1.213 ± 0.023
1.053AsnGlu: 1.053 ± 0.021
0.665AsnPhe: 0.665 ± 0.017
2.262AsnGly: 2.262 ± 0.033
0.512AsnHis: 0.512 ± 0.014
0.749AsnIle: 0.749 ± 0.018
0.486AsnLys: 0.486 ± 0.013
2.286AsnLeu: 2.286 ± 0.028
0.317AsnMet: 0.317 ± 0.011
0.586AsnAsn: 0.586 ± 0.016
1.746AsnPro: 1.746 ± 0.024
0.787AsnGln: 0.787 ± 0.02
1.562AsnArg: 1.562 ± 0.029
1.216AsnSer: 1.216 ± 0.026
1.325AsnThr: 1.325 ± 0.023
1.641AsnVal: 1.641 ± 0.026
0.402AsnTrp: 0.402 ± 0.013
0.536AsnTyr: 0.536 ± 0.015
0.0AsnXaa: 0.0 ± 0.0
Pro
7.606ProAla: 7.606 ± 0.059
0.3ProCys: 0.3 ± 0.011
4.425ProAsp: 4.425 ± 0.039
3.68ProGlu: 3.68 ± 0.042
1.554ProPhe: 1.554 ± 0.024
5.735ProGly: 5.735 ± 0.047
1.134ProHis: 1.134 ± 0.022
2.035ProIle: 2.035 ± 0.031
1.339ProLys: 1.339 ± 0.025
4.888ProLeu: 4.888 ± 0.043
1.03ProMet: 1.03 ± 0.019
1.291ProAsn: 1.291 ± 0.024
3.235ProPro: 3.235 ± 0.049
1.678ProGln: 1.678 ± 0.036
3.306ProArg: 3.306 ± 0.037
3.293ProSer: 3.293 ± 0.038
3.935ProThr: 3.935 ± 0.047
4.923ProVal: 4.923 ± 0.048
0.971ProTrp: 0.971 ± 0.019
1.339ProTyr: 1.339 ± 0.023
0.0ProXaa: 0.0 ± 0.0
Gln
4.096GlnAla: 4.096 ± 0.046
0.157GlnCys: 0.157 ± 0.008
1.349GlnAsp: 1.349 ± 0.023
1.304GlnGlu: 1.304 ± 0.023
0.903GlnPhe: 0.903 ± 0.017
2.123GlnGly: 2.123 ± 0.032
0.714GlnHis: 0.714 ± 0.018
1.339GlnIle: 1.339 ± 0.022
0.671GlnLys: 0.671 ± 0.016
3.667GlnLeu: 3.667 ± 0.039
0.513GlnMet: 0.513 ± 0.013
0.689GlnAsn: 0.689 ± 0.016
2.123GlnPro: 2.123 ± 0.039
1.471GlnGln: 1.471 ± 0.029
2.579GlnArg: 2.579 ± 0.035
1.469GlnSer: 1.469 ± 0.023
1.798GlnThr: 1.798 ± 0.026
2.924GlnVal: 2.924 ± 0.032
0.537GlnTrp: 0.537 ± 0.013
0.858GlnTyr: 0.858 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
7.949ArgAla: 7.949 ± 0.067
0.517ArgCys: 0.517 ± 0.013
4.02ArgAsp: 4.02 ± 0.041
4.034ArgGlu: 4.034 ± 0.046
2.364ArgPhe: 2.364 ± 0.027
5.05ArgGly: 5.05 ± 0.045
1.669ArgHis: 1.669 ± 0.028
3.462ArgIle: 3.462 ± 0.034
1.711ArgLys: 1.711 ± 0.025
8.046ArgLeu: 8.046 ± 0.055
1.682ArgMet: 1.682 ± 0.024
1.569ArgAsn: 1.569 ± 0.023
4.238ArgPro: 4.238 ± 0.049
2.426ArgGln: 2.426 ± 0.033
6.95ArgArg: 6.95 ± 0.062
4.161ArgSer: 4.161 ± 0.042
4.637ArgThr: 4.637 ± 0.05
5.404ArgVal: 5.404 ± 0.049
1.402ArgTrp: 1.402 ± 0.021
1.802ArgTyr: 1.802 ± 0.025
0.0ArgXaa: 0.0 ± 0.0
Ser
6.635SerAla: 6.635 ± 0.056
0.394SerCys: 0.394 ± 0.013
2.938SerAsp: 2.938 ± 0.035
2.49SerGlu: 2.49 ± 0.028
1.743SerPhe: 1.743 ± 0.025
5.814SerGly: 5.814 ± 0.053
0.963SerHis: 0.963 ± 0.017
2.131SerIle: 2.131 ± 0.029
1.273SerLys: 1.273 ± 0.025
5.115SerLeu: 5.115 ± 0.044
1.243SerMet: 1.243 ± 0.02
1.203SerAsn: 1.203 ± 0.022
3.19SerPro: 3.19 ± 0.033
1.486SerGln: 1.486 ± 0.022
3.766SerArg: 3.766 ± 0.038
3.377SerSer: 3.377 ± 0.038
3.793SerThr: 3.793 ± 0.05
4.528SerVal: 4.528 ± 0.042
1.06SerTrp: 1.06 ± 0.02
1.439SerTyr: 1.439 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
8.161ThrAla: 8.161 ± 0.058
0.433ThrCys: 0.433 ± 0.011
3.755ThrAsp: 3.755 ± 0.043
3.31ThrGlu: 3.31 ± 0.038
2.003ThrPhe: 2.003 ± 0.029
6.388ThrGly: 6.388 ± 0.057
1.132ThrHis: 1.132 ± 0.022
2.609ThrIle: 2.609 ± 0.03
1.517ThrLys: 1.517 ± 0.028
5.703ThrLeu: 5.703 ± 0.049
1.094ThrMet: 1.094 ± 0.021
1.389ThrAsn: 1.389 ± 0.025
4.171ThrPro: 4.171 ± 0.05
1.62ThrGln: 1.62 ± 0.026
3.778ThrArg: 3.778 ± 0.038
3.603ThrSer: 3.603 ± 0.043
4.635ThrThr: 4.635 ± 0.056
5.791ThrVal: 5.791 ± 0.053
1.072ThrTrp: 1.072 ± 0.02
1.533ThrTyr: 1.533 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
10.934ValAla: 10.934 ± 0.071
0.632ValCys: 0.632 ± 0.018
5.548ValAsp: 5.548 ± 0.046
4.852ValGlu: 4.852 ± 0.048
2.57ValPhe: 2.57 ± 0.03
6.966ValGly: 6.966 ± 0.063
1.693ValHis: 1.693 ± 0.023
3.662ValIle: 3.662 ± 0.036
1.925ValLys: 1.925 ± 0.025
9.612ValLeu: 9.612 ± 0.067
1.505ValMet: 1.505 ± 0.023
1.92ValAsn: 1.92 ± 0.026
4.828ValPro: 4.828 ± 0.044
2.404ValGln: 2.404 ± 0.032
6.161ValArg: 6.161 ± 0.057
4.649ValSer: 4.649 ± 0.043
5.448ValThr: 5.448 ± 0.044
8.712ValVal: 8.712 ± 0.074
1.089ValTrp: 1.089 ± 0.021
1.613ValTyr: 1.613 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.615TrpAla: 1.615 ± 0.026
0.133TrpCys: 0.133 ± 0.007
0.857TrpAsp: 0.857 ± 0.02
0.643TrpGlu: 0.643 ± 0.015
0.606TrpPhe: 0.606 ± 0.012
1.083TrpGly: 1.083 ± 0.021
0.396TrpHis: 0.396 ± 0.012
0.752TrpIle: 0.752 ± 0.017
0.41TrpLys: 0.41 ± 0.012
1.942TrpLeu: 1.942 ± 0.028
0.352TrpMet: 0.352 ± 0.01
0.513TrpAsn: 0.513 ± 0.013
0.874TrpPro: 0.874 ± 0.017
0.697TrpGln: 0.697 ± 0.016
1.267TrpArg: 1.267 ± 0.021
1.164TrpSer: 1.164 ± 0.021
1.111TrpThr: 1.111 ± 0.022
1.144TrpVal: 1.144 ± 0.018
0.371TrpTrp: 0.371 ± 0.011
0.401TrpTyr: 0.401 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.657TyrAla: 2.657 ± 0.033
0.196TyrCys: 0.196 ± 0.009
1.759TyrAsp: 1.759 ± 0.033
1.19TyrGlu: 1.19 ± 0.018
0.728TyrPhe: 0.728 ± 0.017
2.234TyrGly: 2.234 ± 0.032
0.433TyrHis: 0.433 ± 0.013
0.576TyrIle: 0.576 ± 0.014
0.446TyrLys: 0.446 ± 0.014
2.371TyrLeu: 2.371 ± 0.028
0.24TyrMet: 0.24 ± 0.01
0.508TyrAsn: 0.508 ± 0.017
1.183TyrPro: 1.183 ± 0.019
0.804TyrGln: 0.804 ± 0.018
1.855TyrArg: 1.855 ± 0.028
1.146TyrSer: 1.146 ± 0.022
1.265TyrThr: 1.265 ± 0.026
1.879TyrVal: 1.879 ± 0.024
0.401TyrTrp: 0.401 ± 0.012
0.581TyrTyr: 0.581 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8831 proteins (2844625 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski