Alteraurantiacibacter aquimixticola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Alteraurantiacibacter

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4T3EYD3|A0A4T3EYD3_9SPHN Type VI secretion protein OS=Alteraurantiacibacter aquimixticola OX=2489173 GN=E5222_12340 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.33YY3 pKa = 10.86DD4 pKa = 3.62NLLAASLAAALVAACGGGSSGGGISSTPPPPAAPTPSPTSTSAPTPTPTNSNVGNLVADD63 pKa = 4.12EE64 pKa = 4.69AFSTLSSGSDD74 pKa = 2.79ISLSVIDD81 pKa = 4.79AEE83 pKa = 4.46VGGASVEE90 pKa = 4.05DD91 pKa = 3.53RR92 pKa = 11.84KK93 pKa = 10.89VAISYY98 pKa = 8.49DD99 pKa = 3.55ASTDD103 pKa = 3.33GYY105 pKa = 8.53TVSLGGQGSTFTSAGIIEE123 pKa = 4.21QGEE126 pKa = 4.48YY127 pKa = 10.29PGTTRR132 pKa = 11.84YY133 pKa = 8.24YY134 pKa = 9.21TEE136 pKa = 4.76SGNSSEE142 pKa = 4.88FLTISTRR149 pKa = 11.84GYY151 pKa = 9.31SQNSANQYY159 pKa = 8.83VALGYY164 pKa = 7.42WQRR167 pKa = 11.84NVLNGVQQEE176 pKa = 4.72TEE178 pKa = 3.26FDD180 pKa = 3.55TFVFGFPASSSAVPVSGLGSYY201 pKa = 9.91TIDD204 pKa = 2.82IFGALSVVGTEE215 pKa = 3.96ARR217 pKa = 11.84ALSGSGLLEE226 pKa = 4.31LDD228 pKa = 4.2FGTGAWRR235 pKa = 11.84VRR237 pKa = 11.84TTLGEE242 pKa = 4.2FPLADD247 pKa = 3.47YY248 pKa = 10.52GYY250 pKa = 11.15LSGGSLKK257 pKa = 10.61FNADD261 pKa = 3.08GFVTSNRR268 pKa = 11.84RR269 pKa = 11.84LDD271 pKa = 4.6GIFTYY276 pKa = 10.79TNLDD280 pKa = 3.24GDD282 pKa = 4.35FVSGDD287 pKa = 3.35VSGQFYY293 pKa = 10.81GPAAQEE299 pKa = 3.65VGAAFSGGTGTGAVLNGAFTGKK321 pKa = 9.89RR322 pKa = 11.84DD323 pKa = 3.61NQSRR327 pKa = 11.84AGTLALTNIQGDD339 pKa = 4.43TVVSGYY345 pKa = 10.27LAEE348 pKa = 4.53ALGTIQDD355 pKa = 4.29DD356 pKa = 3.65LTPAFRR362 pKa = 11.84GFYY365 pKa = 10.25GNTYY369 pKa = 7.21TEE371 pKa = 4.25RR372 pKa = 11.84PFTRR376 pKa = 11.84IHH378 pKa = 6.02FQQDD382 pKa = 2.97GTINVQLDD390 pKa = 3.7GALVASIEE398 pKa = 4.07PGEE401 pKa = 4.27LVSSDD406 pKa = 3.04GAYY409 pKa = 10.29DD410 pKa = 3.61VYY412 pKa = 10.92EE413 pKa = 4.31TTTTSAFGTAGLPLTARR430 pKa = 11.84IFKK433 pKa = 10.19PGTANPEE440 pKa = 3.89VQLTYY445 pKa = 11.1SSFGILEE452 pKa = 4.03QEE454 pKa = 4.22ISGSDD459 pKa = 3.43YY460 pKa = 9.64LQQVRR465 pKa = 11.84RR466 pKa = 11.84YY467 pKa = 6.94FTYY470 pKa = 10.54GFDD473 pKa = 3.59TPEE476 pKa = 3.22GHH478 pKa = 6.3MNVRR482 pKa = 11.84SGSASYY488 pKa = 10.86SGIAVGTTTNSSGLAIDD505 pKa = 3.73VDD507 pKa = 4.44GTSSFVVNFSSDD519 pKa = 3.5SYY521 pKa = 11.93SGMLDD526 pKa = 3.66LQADD530 pKa = 4.02DD531 pKa = 4.69GGTIVDD537 pKa = 4.44LGTFEE542 pKa = 4.39FASTITNGIMDD553 pKa = 5.57AANFDD558 pKa = 4.14YY559 pKa = 8.57PTALSASVQSLNTIIPVFYY578 pKa = 10.55GPDD581 pKa = 3.35GSEE584 pKa = 3.37IVAPFSILRR593 pKa = 11.84GPPNAIGTTTITGVAVAIEE612 pKa = 4.09DD613 pKa = 3.57

Molecular weight: 63.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4T3F646|A0A4T3F646_9SPHN Uncharacterized protein OS=Alteraurantiacibacter aquimixticola OX=2489173 GN=E5222_05440 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 7.93VLRR32 pKa = 11.84SRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.51NLCAA44 pKa = 4.54

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3188

0

3188

1045476

40

8505

327.9

35.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.388 ± 0.071

0.85 ± 0.015

6.247 ± 0.066

6.638 ± 0.051

3.689 ± 0.03

8.973 ± 0.053

1.951 ± 0.022

4.955 ± 0.028

2.854 ± 0.042

9.691 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.024

2.724 ± 0.032

5.137 ± 0.035

3.193 ± 0.024

6.746 ± 0.053

5.388 ± 0.032

5.159 ± 0.043

7.078 ± 0.029

1.468 ± 0.018

2.275 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski