Nitratireductor sp. SY7

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Nitratireductor; unclassified Nitratireductor

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4636 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B8KTH6|A0A5B8KTH6_9RHIZ Copper-bind domain-containing protein OS=Nitratireductor sp. SY7 OX=2599600 GN=FQ775_00110 PE=4 SV=1
MM1 pKa = 6.86QPAWNGSSEE10 pKa = 4.11PAGVFDD16 pKa = 5.32LTGDD20 pKa = 3.87PNIDD24 pKa = 3.76GILARR29 pKa = 11.84RR30 pKa = 11.84FWSDD34 pKa = 2.62GSITYY39 pKa = 9.32SFPDD43 pKa = 3.21QASDD47 pKa = 3.46YY48 pKa = 10.6GAYY51 pKa = 10.24SYY53 pKa = 11.95VDD55 pKa = 3.6GNGVTHH61 pKa = 7.08FPTATFQQVLAAQQTAIHH79 pKa = 6.43FAMSVEE85 pKa = 3.94NGPLASQGFSVEE97 pKa = 3.94GFTNLDD103 pKa = 3.25VGFNGTTTGNATMRR117 pKa = 11.84FGRR120 pKa = 11.84SDD122 pKa = 3.64DD123 pKa = 3.89AQPTAFAFYY132 pKa = 9.69PSSSNVGGDD141 pKa = 3.02VWLGTQVGGSNLSNPAAGNYY161 pKa = 8.54AWATILHH168 pKa = 6.68EE169 pKa = 4.88IGHH172 pKa = 6.13GLGLKK177 pKa = 9.73HH178 pKa = 6.0GHH180 pKa = 6.8AGDD183 pKa = 4.06SGFPNANPNVLPSNVDD199 pKa = 2.9AMEE202 pKa = 3.99YY203 pKa = 10.72SIMTYY208 pKa = 10.2RR209 pKa = 11.84SFINGPTNGYY219 pKa = 7.63TNEE222 pKa = 4.31TNGFAQTFMMLDD234 pKa = 3.61IAALQAMYY242 pKa = 10.39GADD245 pKa = 4.42FSSQSNNTDD254 pKa = 2.84TVYY257 pKa = 10.67KK258 pKa = 9.75WDD260 pKa = 3.83PTSGDD265 pKa = 3.54TLINGQVGIDD275 pKa = 3.28AAANRR280 pKa = 11.84IFATIWDD287 pKa = 4.04GGGVDD292 pKa = 3.97TYY294 pKa = 11.59DD295 pKa = 3.19LSSYY299 pKa = 9.54TGNLMLDD306 pKa = 3.9LRR308 pKa = 11.84PGEE311 pKa = 4.13EE312 pKa = 4.34SLFSTTQQAHH322 pKa = 6.38LGSGNFASGNIYY334 pKa = 10.53NALQFQGDD342 pKa = 3.8ARR344 pKa = 11.84SLIEE348 pKa = 3.9NAIGGTGNDD357 pKa = 4.11TITGNAANNSLFGGQGDD374 pKa = 4.05DD375 pKa = 3.8TLSGGNGNDD384 pKa = 3.76LLAGGAGVDD393 pKa = 3.78QINGEE398 pKa = 4.35GGVDD402 pKa = 3.26TADD405 pKa = 3.71YY406 pKa = 9.74SASAASVNVNLSTGTGSGGDD426 pKa = 3.65AQGDD430 pKa = 3.94TLTGIEE436 pKa = 4.4NLIGSNVAGTDD447 pKa = 3.31VLTGDD452 pKa = 3.78GTANHH457 pKa = 6.32IQGLAGNDD465 pKa = 3.09ILVGLGGADD474 pKa = 2.87ILVGGTGVDD483 pKa = 3.31TADD486 pKa = 3.59YY487 pKa = 9.23SASGGRR493 pKa = 11.84INVNLTTGAGLGGDD507 pKa = 3.44AHH509 pKa = 7.47GDD511 pKa = 3.54TLSGIEE517 pKa = 4.33NLIGTNIAMTDD528 pKa = 4.29FLTGDD533 pKa = 3.36AGANHH538 pKa = 6.37IQGLAGDD545 pKa = 4.65DD546 pKa = 4.56LIAGLGGADD555 pKa = 3.57VLIGGSGVDD564 pKa = 3.29TADD567 pKa = 3.54YY568 pKa = 10.12SASASRR574 pKa = 11.84IAVNLSTGYY583 pKa = 10.68AAGGDD588 pKa = 3.6AHH590 pKa = 7.44GDD592 pKa = 3.55TLSGIEE598 pKa = 4.3NLIGTNSSLTDD609 pKa = 3.97FLTGDD614 pKa = 3.48AGANHH619 pKa = 6.37IQGLAGDD626 pKa = 4.65DD627 pKa = 4.56LIAGLGGADD636 pKa = 3.57VLIGGSGVDD645 pKa = 3.29TADD648 pKa = 3.54YY649 pKa = 10.12SASASRR655 pKa = 11.84IAVNLSTGYY664 pKa = 10.68AAGGDD669 pKa = 3.6AHH671 pKa = 7.44GDD673 pKa = 3.55TLSGIEE679 pKa = 4.26NLIGTNVALTDD690 pKa = 4.06FLTGNSLANRR700 pKa = 11.84IEE702 pKa = 4.33GGAGDD707 pKa = 5.04DD708 pKa = 5.0LIAGLGGADD717 pKa = 3.57VLIGGSGVDD726 pKa = 3.29TADD729 pKa = 3.46YY730 pKa = 9.82SASGGRR736 pKa = 11.84IAVNLSTGYY745 pKa = 10.68AAGGDD750 pKa = 3.6AHH752 pKa = 7.44GDD754 pKa = 3.55TLSGIEE760 pKa = 4.26NLIGTNVALTDD771 pKa = 4.23FLTGDD776 pKa = 3.29AGANRR781 pKa = 11.84IEE783 pKa = 4.47GLAGDD788 pKa = 4.81DD789 pKa = 4.21EE790 pKa = 5.38INGKK794 pKa = 9.66EE795 pKa = 3.97GADD798 pKa = 3.13IFVGGGGDD806 pKa = 3.82DD807 pKa = 4.95LFIFDD812 pKa = 4.25TALGAGNIDD821 pKa = 4.53AILDD825 pKa = 3.67FVVADD830 pKa = 3.92DD831 pKa = 4.08MVRR834 pKa = 11.84LASSIFTGLSVGTLTAAAFRR854 pKa = 11.84VGAAAADD861 pKa = 3.66ADD863 pKa = 4.9DD864 pKa = 5.3RR865 pKa = 11.84IIYY868 pKa = 9.53NAGSGGLFFDD878 pKa = 3.86VDD880 pKa = 3.81GNGAQGQVQFASLSTGLALTNDD902 pKa = 3.84NFLVAA907 pKa = 5.3

Molecular weight: 90.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B8L058|A0A5B8L058_9RHIZ (S)-ureidoglycine aminohydrolase OS=Nitratireductor sp. SY7 OX=2599600 GN=FQ775_14065 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.68GGRR28 pKa = 11.84RR29 pKa = 11.84VVAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84NRR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4636

0

4636

1446749

32

2124

312.1

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.926 ± 0.052

0.813 ± 0.011

5.844 ± 0.035

6.186 ± 0.037

3.925 ± 0.026

8.804 ± 0.034

2.018 ± 0.016

5.226 ± 0.026

3.097 ± 0.032

9.825 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.017

2.491 ± 0.022

4.983 ± 0.028

2.704 ± 0.018

7.376 ± 0.037

5.199 ± 0.021

5.031 ± 0.022

7.558 ± 0.028

1.311 ± 0.016

2.168 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski