Amino acid dipepetide frequency for Streptomyces sp. HPH0547

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.399AlaAla: 23.399 ± 0.206	1.189AlaCys: 1.189 ± 0.027	8.326AlaAsp: 8.326 ± 0.074	9.099AlaGlu: 9.099 ± 0.081	3.361AlaPhe: 3.361 ± 0.042	14.875AlaGly: 14.875 ± 0.105	3.214AlaHis: 3.214 ± 0.042	2.581AlaIle: 2.581 ± 0.043	2.589AlaLys: 2.589 ± 0.051	14.968AlaLeu: 14.968 ± 0.114	2.369AlaMet: 2.369 ± 0.036	1.633AlaAsn: 1.633 ± 0.028	8.318AlaPro: 8.318 ± 0.092	4.077AlaGln: 4.077 ± 0.053	11.732AlaArg: 11.732 ± 0.097	5.543AlaSer: 5.543 ± 0.063	6.801AlaThr: 6.801 ± 0.069	12.335AlaVal: 12.335 ± 0.089	1.808AlaTrp: 1.808 ± 0.031	2.466AlaTyr: 2.466 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	1.233CysAla: 1.233 ± 0.028	0.104CysCys: 0.104 ± 0.008	0.494CysAsp: 0.494 ± 0.016	0.408CysGlu: 0.408 ± 0.016	0.217CysPhe: 0.217 ± 0.01	1.022CysGly: 1.022 ± 0.026	0.192CysHis: 0.192 ± 0.009	0.146CysIle: 0.146 ± 0.007	0.118CysLys: 0.118 ± 0.007	0.764CysLeu: 0.764 ± 0.021	0.122CysMet: 0.122 ± 0.007	0.124CysAsn: 0.124 ± 0.007	0.525CysPro: 0.525 ± 0.017	0.179CysGln: 0.179 ± 0.01	0.696CysArg: 0.696 ± 0.018	0.4CysSer: 0.4 ± 0.013	0.544CysThr: 0.544 ± 0.017	0.715CysVal: 0.715 ± 0.018	0.142CysTrp: 0.142 ± 0.008	0.168CysTyr: 0.168 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.866AspAla: 7.866 ± 0.063	0.401AspCys: 0.401 ± 0.015	3.425AspAsp: 3.425 ± 0.046	4.053AspGlu: 4.053 ± 0.052	1.486AspPhe: 1.486 ± 0.026	6.835AspGly: 6.835 ± 0.069	1.346AspHis: 1.346 ± 0.028	1.743AspIle: 1.743 ± 0.036	1.135AspLys: 1.135 ± 0.027	5.364AspLeu: 5.364 ± 0.052	0.803AspMet: 0.803 ± 0.02	0.85AspAsn: 0.85 ± 0.021	4.451AspPro: 4.451 ± 0.054	1.37AspGln: 1.37 ± 0.025	4.881AspArg: 4.881 ± 0.051	2.422AspSer: 2.422 ± 0.04	3.083AspThr: 3.083 ± 0.042	4.311AspVal: 4.311 ± 0.045	0.991AspTrp: 0.991 ± 0.022	0.987AspTyr: 0.987 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.878GluAla: 7.878 ± 0.083	0.387GluCys: 0.387 ± 0.016	2.941GluAsp: 2.941 ± 0.043	4.138GluGlu: 4.138 ± 0.049	1.308GluPhe: 1.308 ± 0.024	4.867GluGly: 4.867 ± 0.047	1.59GluHis: 1.59 ± 0.027	1.927GluIle: 1.927 ± 0.035	1.467GluLys: 1.467 ± 0.033	6.591GluLeu: 6.591 ± 0.054	0.935GluMet: 0.935 ± 0.021	0.978GluAsn: 0.978 ± 0.022	3.715GluPro: 3.715 ± 0.053	2.343GluGln: 2.343 ± 0.045	6.11GluArg: 6.11 ± 0.064	2.445GluSer: 2.445 ± 0.043	3.181GluThr: 3.181 ± 0.04	4.298GluVal: 4.298 ± 0.047	0.803GluTrp: 0.803 ± 0.021	1.026GluTyr: 1.026 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.628PheAla: 3.628 ± 0.04	0.278PheCys: 0.278 ± 0.013	1.789PheAsp: 1.789 ± 0.029	1.275PheGlu: 1.275 ± 0.024	0.864PhePhe: 0.864 ± 0.022	2.835PheGly: 2.835 ± 0.045	0.601PheHis: 0.601 ± 0.016	0.588PheIle: 0.588 ± 0.017	0.435PheLys: 0.435 ± 0.015	2.517PheLeu: 2.517 ± 0.039	0.349PheMet: 0.349 ± 0.013	0.465PheAsn: 0.465 ± 0.016	1.341PhePro: 1.341 ± 0.025	0.601PheGln: 0.601 ± 0.017	1.834PheArg: 1.834 ± 0.035	1.341PheSer: 1.341 ± 0.024	1.873PheThr: 1.873 ± 0.034	2.114PheVal: 2.114 ± 0.034	0.385PheTrp: 0.385 ± 0.013	0.503PheTyr: 0.503 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	12.274GlyAla: 12.274 ± 0.089	0.883GlyCys: 0.883 ± 0.023	5.311GlyAsp: 5.311 ± 0.057	5.665GlyGlu: 5.665 ± 0.056	2.673GlyPhe: 2.673 ± 0.038	9.962GlyGly: 9.962 ± 0.1	2.531GlyHis: 2.531 ± 0.04	3.184GlyIle: 3.184 ± 0.036	2.507GlyLys: 2.507 ± 0.042	9.468GlyLeu: 9.468 ± 0.076	2.147GlyMet: 2.147 ± 0.036	1.668GlyAsn: 1.668 ± 0.03	6.463GlyPro: 6.463 ± 0.083	2.83GlyGln: 2.83 ± 0.044	8.569GlyArg: 8.569 ± 0.078	5.425GlySer: 5.425 ± 0.061	7.085GlyThr: 7.085 ± 0.074	7.324GlyVal: 7.324 ± 0.068	1.688GlyTrp: 1.688 ± 0.028	2.157GlyTyr: 2.157 ± 0.035	0.0GlyXaa: 0.0 ± 0.0
His	2.838HisAla: 2.838 ± 0.041	0.229HisCys: 0.229 ± 0.011	1.343HisAsp: 1.343 ± 0.028	1.308HisGlu: 1.308 ± 0.023	0.615HisPhe: 0.615 ± 0.016	2.681HisGly: 2.681 ± 0.04	0.73HisHis: 0.73 ± 0.023	0.645HisIle: 0.645 ± 0.018	0.396HisLys: 0.396 ± 0.016	2.495HisLeu: 2.495 ± 0.033	0.369HisMet: 0.369 ± 0.014	0.355HisAsn: 0.355 ± 0.013	1.986HisPro: 1.986 ± 0.035	0.684HisGln: 0.684 ± 0.019	2.336HisArg: 2.336 ± 0.037	1.077HisSer: 1.077 ± 0.023	1.491HisThr: 1.491 ± 0.028	1.668HisVal: 1.668 ± 0.025	0.381HisTrp: 0.381 ± 0.013	0.482HisTyr: 0.482 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	3.929IleAla: 3.929 ± 0.055	0.253IleCys: 0.253 ± 0.011	1.863IleAsp: 1.863 ± 0.034	1.708IleGlu: 1.708 ± 0.035	0.577IlePhe: 0.577 ± 0.019	3.049IleGly: 3.049 ± 0.049	0.515IleHis: 0.515 ± 0.015	0.686IleIle: 0.686 ± 0.018	0.599IleLys: 0.599 ± 0.02	1.937IleLeu: 1.937 ± 0.036	0.35IleMet: 0.35 ± 0.011	0.568IleAsn: 0.568 ± 0.017	1.548IlePro: 1.548 ± 0.029	0.6IleGln: 0.6 ± 0.018	2.008IleArg: 2.008 ± 0.033	1.436IleSer: 1.436 ± 0.028	1.791IleThr: 1.791 ± 0.032	2.283IleVal: 2.283 ± 0.035	0.287IleTrp: 0.287 ± 0.012	0.412IleTyr: 0.412 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	2.628LysAla: 2.628 ± 0.048	0.111LysCys: 0.111 ± 0.007	1.244LysAsp: 1.244 ± 0.028	1.21LysGlu: 1.21 ± 0.029	0.41LysPhe: 0.41 ± 0.013	1.809LysGly: 1.809 ± 0.041	0.437LysHis: 0.437 ± 0.014	0.695LysIle: 0.695 ± 0.019	0.924LysLys: 0.924 ± 0.029	1.882LysLeu: 1.882 ± 0.034	0.373LysMet: 0.373 ± 0.013	0.479LysAsn: 0.479 ± 0.017	1.331LysPro: 1.331 ± 0.03	0.671LysGln: 0.671 ± 0.022	1.411LysArg: 1.411 ± 0.029	1.005LysSer: 1.005 ± 0.023	1.175LysThr: 1.175 ± 0.025	1.635LysVal: 1.635 ± 0.033	0.239LysTrp: 0.239 ± 0.011	0.391LysTyr: 0.391 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.464LeuAla: 15.464 ± 0.119	0.916LeuCys: 0.916 ± 0.025	6.311LeuAsp: 6.311 ± 0.059	4.839LeuGlu: 4.839 ± 0.055	2.526LeuPhe: 2.526 ± 0.037	9.174LeuGly: 9.174 ± 0.064	2.422LeuHis: 2.422 ± 0.038	2.683LeuIle: 2.683 ± 0.048	1.681LeuLys: 1.681 ± 0.032	11.482LeuLeu: 11.482 ± 0.097	1.533LeuMet: 1.533 ± 0.026	1.424LeuAsn: 1.424 ± 0.028	6.786LeuPro: 6.786 ± 0.063	1.849LeuGln: 1.849 ± 0.03	9.224LeuArg: 9.224 ± 0.074	5.147LeuSer: 5.147 ± 0.05	6.691LeuThr: 6.691 ± 0.06	8.674LeuVal: 8.674 ± 0.073	1.299LeuTrp: 1.299 ± 0.028	1.717LeuTyr: 1.717 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.213MetAla: 2.213 ± 0.035	0.148MetCys: 0.148 ± 0.009	0.845MetAsp: 0.845 ± 0.022	0.752MetGlu: 0.752 ± 0.017	0.409MetPhe: 0.409 ± 0.016	1.286MetGly: 1.286 ± 0.025	0.355MetHis: 0.355 ± 0.012	0.569MetIle: 0.569 ± 0.02	0.373MetLys: 0.373 ± 0.013	1.659MetLeu: 1.659 ± 0.031	0.279MetMet: 0.279 ± 0.013	0.41MetAsn: 0.41 ± 0.013	1.149MetPro: 1.149 ± 0.021	0.399MetGln: 0.399 ± 0.013	1.483MetArg: 1.483 ± 0.024	1.276MetSer: 1.276 ± 0.026	1.481MetThr: 1.481 ± 0.025	1.197MetVal: 1.197 ± 0.022	0.223MetTrp: 0.223 ± 0.01	0.298MetTyr: 0.298 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.046AsnAla: 2.046 ± 0.034	0.149AsnCys: 0.149 ± 0.008	0.818AsnAsp: 0.818 ± 0.021	0.75AsnGlu: 0.75 ± 0.018	0.391AsnPhe: 0.391 ± 0.012	1.712AsnGly: 1.712 ± 0.03	0.339AsnHis: 0.339 ± 0.013	0.544AsnIle: 0.544 ± 0.016	0.352AsnLys: 0.352 ± 0.014	1.438AsnLeu: 1.438 ± 0.029	0.268AsnMet: 0.268 ± 0.009	0.344AsnAsn: 0.344 ± 0.013	1.202AsnPro: 1.202 ± 0.025	0.433AsnGln: 0.433 ± 0.014	1.202AsnArg: 1.202 ± 0.026	0.779AsnSer: 0.779 ± 0.018	0.977AsnThr: 0.977 ± 0.024	1.162AsnVal: 1.162 ± 0.027	0.245AsnTrp: 0.245 ± 0.01	0.359AsnTyr: 0.359 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.949ProAla: 9.949 ± 0.106	0.404ProCys: 0.404 ± 0.014	4.754ProAsp: 4.754 ± 0.056	4.603ProGlu: 4.603 ± 0.063	1.534ProPhe: 1.534 ± 0.028	8.119ProGly: 8.119 ± 0.083	1.817ProHis: 1.817 ± 0.036	0.955ProIle: 0.955 ± 0.022	1.102ProLys: 1.102 ± 0.027	5.584ProLeu: 5.584 ± 0.057	0.958ProMet: 0.958 ± 0.02	0.772ProAsn: 0.772 ± 0.019	4.336ProPro: 4.336 ± 0.075	2.149ProGln: 2.149 ± 0.049	4.759ProArg: 4.759 ± 0.063	3.338ProSer: 3.338 ± 0.043	2.976ProThr: 2.976 ± 0.042	5.67ProVal: 5.67 ± 0.066	0.93ProTrp: 0.93 ± 0.026	1.335ProTyr: 1.335 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.755GlnAla: 3.755 ± 0.051	0.177GlnCys: 0.177 ± 0.01	1.448GlnAsp: 1.448 ± 0.027	1.64GlnGlu: 1.64 ± 0.035	0.624GlnPhe: 0.624 ± 0.018	2.378GlnGly: 2.378 ± 0.043	0.718GlnHis: 0.718 ± 0.02	0.948GlnIle: 0.948 ± 0.023	0.533GlnLys: 0.533 ± 0.018	2.932GlnLeu: 2.932 ± 0.038	0.466GlnMet: 0.466 ± 0.018	0.434GlnAsn: 0.434 ± 0.015	1.993GlnPro: 1.993 ± 0.052	1.353GlnGln: 1.353 ± 0.038	2.504GlnArg: 2.504 ± 0.038	1.12GlnSer: 1.12 ± 0.024	1.255GlnThr: 1.255 ± 0.026	2.252GlnVal: 2.252 ± 0.035	0.413GlnTrp: 0.413 ± 0.013	0.532GlnTyr: 0.532 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	10.857ArgAla: 10.857 ± 0.095	0.682ArgCys: 0.682 ± 0.019	4.467ArgAsp: 4.467 ± 0.046	5.307ArgGlu: 5.307 ± 0.06	2.309ArgPhe: 2.309 ± 0.03	6.517ArgGly: 6.517 ± 0.061	2.298ArgHis: 2.298 ± 0.036	3.016ArgIle: 3.016 ± 0.036	1.83ArgLys: 1.83 ± 0.032	9.311ArgLeu: 9.311 ± 0.075	1.838ArgMet: 1.838 ± 0.031	1.369ArgAsn: 1.369 ± 0.027	5.846ArgPro: 5.846 ± 0.068	2.56ArgGln: 2.56 ± 0.042	8.708ArgArg: 8.708 ± 0.08	4.223ArgSer: 4.223 ± 0.047	6.034ArgThr: 6.034 ± 0.059	5.991ArgVal: 5.991 ± 0.056	1.429ArgTrp: 1.429 ± 0.026	1.844ArgTyr: 1.844 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.721SerAla: 6.721 ± 0.066	0.409SerCys: 0.409 ± 0.013	2.477SerAsp: 2.477 ± 0.04	2.305SerGlu: 2.305 ± 0.046	1.432SerPhe: 1.432 ± 0.024	6.102SerGly: 6.102 ± 0.064	0.994SerHis: 0.994 ± 0.019	1.012SerIle: 1.012 ± 0.023	0.87SerLys: 0.87 ± 0.023	4.522SerLeu: 4.522 ± 0.045	0.943SerMet: 0.943 ± 0.02	0.697SerAsn: 0.697 ± 0.017	3.394SerPro: 3.394 ± 0.044	1.167SerGln: 1.167 ± 0.026	3.94SerArg: 3.94 ± 0.045	2.722SerSer: 2.722 ± 0.049	2.746SerThr: 2.746 ± 0.037	3.95SerVal: 3.95 ± 0.04	0.817SerTrp: 0.817 ± 0.02	1.078SerTyr: 1.078 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.309ThrAla: 9.309 ± 0.079	0.429ThrCys: 0.429 ± 0.015	3.541ThrAsp: 3.541 ± 0.04	3.261ThrGlu: 3.261 ± 0.04	1.52ThrPhe: 1.52 ± 0.029	7.22ThrGly: 7.22 ± 0.061	1.219ThrHis: 1.219 ± 0.025	1.289ThrIle: 1.289 ± 0.03	0.998ThrLys: 0.998 ± 0.024	5.581ThrLeu: 5.581 ± 0.05	0.899ThrMet: 0.899 ± 0.021	0.84ThrAsn: 0.84 ± 0.022	4.218ThrPro: 4.218 ± 0.045	1.236ThrGln: 1.236 ± 0.024	4.185ThrArg: 4.185 ± 0.043	2.921ThrSer: 2.921 ± 0.04	3.649ThrThr: 3.649 ± 0.056	6.131ThrVal: 6.131 ± 0.057	0.813ThrTrp: 0.813 ± 0.019	1.173ThrTyr: 1.173 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.389ValAla: 10.389 ± 0.088	0.848ValCys: 0.848 ± 0.02	4.23ValAsp: 4.23 ± 0.05	4.6ValGlu: 4.6 ± 0.064	2.331ValPhe: 2.331 ± 0.035	6.176ValGly: 6.176 ± 0.056	2.024ValHis: 2.024 ± 0.029	2.34ValIle: 2.34 ± 0.041	1.476ValLys: 1.476 ± 0.03	9.596ValLeu: 9.596 ± 0.077	1.297ValMet: 1.297 ± 0.027	1.459ValAsn: 1.459 ± 0.028	5.659ValPro: 5.659 ± 0.065	1.855ValGln: 1.855 ± 0.031	7.665ValArg: 7.665 ± 0.069	4.02ValSer: 4.02 ± 0.042	5.633ValThr: 5.633 ± 0.055	7.558ValVal: 7.558 ± 0.079	1.109ValTrp: 1.109 ± 0.023	1.416ValTyr: 1.416 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.673TrpAla: 1.673 ± 0.033	0.159TrpCys: 0.159 ± 0.008	0.854TrpAsp: 0.854 ± 0.02	0.809TrpGlu: 0.809 ± 0.023	0.449TrpPhe: 0.449 ± 0.015	1.053TrpGly: 1.053 ± 0.025	0.386TrpHis: 0.386 ± 0.015	0.466TrpIle: 0.466 ± 0.016	0.333TrpLys: 0.333 ± 0.014	1.796TrpLeu: 1.796 ± 0.03	0.273TrpMet: 0.273 ± 0.011	0.321TrpAsn: 0.321 ± 0.013	0.762TrpPro: 0.762 ± 0.02	0.595TrpGln: 0.595 ± 0.017	1.425TrpArg: 1.425 ± 0.027	0.817TrpSer: 0.817 ± 0.02	0.905TrpThr: 0.905 ± 0.02	0.925TrpVal: 0.925 ± 0.019	0.33TrpTrp: 0.33 ± 0.013	0.337TrpTyr: 0.337 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.634TyrAla: 2.634 ± 0.037	0.162TyrCys: 0.162 ± 0.009	1.319TyrAsp: 1.319 ± 0.027	1.197TyrGlu: 1.197 ± 0.026	0.581TyrPhe: 0.581 ± 0.017	2.14TyrGly: 2.14 ± 0.033	0.378TyrHis: 0.378 ± 0.014	0.38TyrIle: 0.38 ± 0.013	0.343TyrLys: 0.343 ± 0.014	1.955TyrLeu: 1.955 ± 0.03	0.231TyrMet: 0.231 ± 0.01	0.317TyrAsn: 0.317 ± 0.013	1.019TyrPro: 1.019 ± 0.023	0.525TyrGln: 0.525 ± 0.016	1.766TyrArg: 1.766 ± 0.033	0.843TyrSer: 0.843 ± 0.022	1.064TyrThr: 1.064 ± 0.022	1.491TyrVal: 1.491 ± 0.024	0.337TyrTrp: 0.337 ± 0.012	0.393TyrTyr: 0.393 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6620 proteins (2249410 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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