Staphylococcus phage IME1323_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; unclassified Azeredovirinae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JNQ0|A0A1W6JNQ0_9CAUD ATPase OS=Staphylococcus phage IME1323_01 OX=1965487 PE=4 SV=1
MM1 pKa = 7.22VKK3 pKa = 9.83IKK5 pKa = 10.23EE6 pKa = 4.11IQTDD10 pKa = 3.92GTYY13 pKa = 10.24TIYY16 pKa = 10.97DD17 pKa = 3.8SNDD20 pKa = 2.65NSINITKK27 pKa = 10.6EE28 pKa = 3.45EE29 pKa = 3.98FEE31 pKa = 4.7EE32 pKa = 4.12IKK34 pKa = 10.59QYY36 pKa = 11.19SIDD39 pKa = 3.56SEE41 pKa = 5.01YY42 pKa = 11.5NFLSSEE48 pKa = 4.13DD49 pKa = 3.75TQAA52 pKa = 4.25

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JNR2|A0A1W6JNR2_9CAUD Uncharacterized protein OS=Staphylococcus phage IME1323_01 OX=1965487 PE=4 SV=1
MM1 pKa = 7.44AQRR4 pKa = 11.84NNYY7 pKa = 8.54PNSSWKK13 pKa = 9.98IYY15 pKa = 10.41RR16 pKa = 11.84NTPSFVPKK24 pKa = 10.4PGDD27 pKa = 3.52IVCWTYY33 pKa = 11.15GWAGHH38 pKa = 5.53TGIVVGPSDD47 pKa = 3.58KK48 pKa = 10.35KK49 pKa = 8.97TFRR52 pKa = 11.84TVDD55 pKa = 3.57QNWFGANQYY64 pKa = 9.97SGSRR68 pKa = 11.84AAYY71 pKa = 9.65VKK73 pKa = 9.34HH74 pKa = 6.33TYY76 pKa = 10.9NGMGGNIYY84 pKa = 10.25FIRR87 pKa = 11.84PPYY90 pKa = 9.4KK91 pKa = 10.31ASNNSAPAKK100 pKa = 10.33KK101 pKa = 9.76QDD103 pKa = 3.51TNKK106 pKa = 9.64VVTKK110 pKa = 9.91KK111 pKa = 10.5KK112 pKa = 10.25KK113 pKa = 8.07RR114 pKa = 11.84TEE116 pKa = 3.51ISFTIDD122 pKa = 3.29DD123 pKa = 4.43NEE125 pKa = 4.38TIYY128 pKa = 10.87PEE130 pKa = 4.83FIPHH134 pKa = 6.56QIVLGNDD141 pKa = 3.09RR142 pKa = 11.84GHH144 pKa = 5.99SPKK147 pKa = 10.78GVTVRR152 pKa = 11.84NAGTMCSVQDD162 pKa = 3.71MYY164 pKa = 11.44FDD166 pKa = 3.47RR167 pKa = 11.84QKK169 pKa = 10.92YY170 pKa = 9.96IKK172 pKa = 10.38DD173 pKa = 3.26KK174 pKa = 10.54EE175 pKa = 4.29YY176 pKa = 9.64PHH178 pKa = 6.96YY179 pKa = 10.66FIDD182 pKa = 5.64RR183 pKa = 11.84NHH185 pKa = 6.03IWQPRR190 pKa = 11.84LEE192 pKa = 4.41TYY194 pKa = 8.16EE195 pKa = 4.37VPSDD199 pKa = 3.7PQNLVIEE206 pKa = 4.56VCGDD210 pKa = 3.47LSDD213 pKa = 4.43SKK215 pKa = 11.82NDD217 pKa = 4.09FILNEE222 pKa = 3.93LHH224 pKa = 7.26AMIFVTQRR232 pKa = 11.84MKK234 pKa = 10.72FHH236 pKa = 6.92NISLKK241 pKa = 10.44KK242 pKa = 10.58SSINVKK248 pKa = 10.42DD249 pKa = 3.66PVWRR253 pKa = 11.84SIYY256 pKa = 10.5EE257 pKa = 4.04HH258 pKa = 7.09GDD260 pKa = 2.65WNTALKK266 pKa = 10.64GKK268 pKa = 10.02APKK271 pKa = 10.04KK272 pKa = 10.69VIDD275 pKa = 3.8KK276 pKa = 9.7TVEE279 pKa = 3.54ALMYY283 pKa = 10.45LYY285 pKa = 10.68GHH287 pKa = 7.13RR288 pKa = 11.84KK289 pKa = 7.16TLLTEE294 pKa = 4.07IPRR297 pKa = 11.84DD298 pKa = 3.57KK299 pKa = 10.36ITTRR303 pKa = 11.84TIRR306 pKa = 11.84VSVPSSSSKK315 pKa = 10.72NSNSTSSSSKK325 pKa = 10.68KK326 pKa = 10.4NSTSHH331 pKa = 6.21NSSHH335 pKa = 6.05STTKK339 pKa = 10.42KK340 pKa = 10.54KK341 pKa = 9.45KK342 pKa = 6.19TTNKK346 pKa = 9.66KK347 pKa = 7.93QNQVIVEE354 pKa = 3.97HH355 pKa = 6.4SKK357 pKa = 9.14YY358 pKa = 9.58TFNQAVNIQSRR369 pKa = 11.84LSPQINYY376 pKa = 9.26GVGWYY381 pKa = 9.51NATRR385 pKa = 11.84SQTLNAMNSLQIWNSGTQKK404 pKa = 10.8YY405 pKa = 9.08QMLNLGKK412 pKa = 9.63YY413 pKa = 9.45QGISVTALNKK423 pKa = 9.62ILRR426 pKa = 11.84GKK428 pKa = 10.68GSLSGQGKK436 pKa = 9.31AVAYY440 pKa = 9.94ACKK443 pKa = 10.06KK444 pKa = 9.61YY445 pKa = 10.83NLNEE449 pKa = 3.84IYY451 pKa = 10.47LISHH455 pKa = 7.21AFLEE459 pKa = 4.57SGYY462 pKa = 9.98GRR464 pKa = 11.84SYY466 pKa = 10.73FSSGRR471 pKa = 11.84AGVYY475 pKa = 10.16NYY477 pKa = 10.44FGIGAFDD484 pKa = 4.28SNPNNAINYY493 pKa = 7.55ARR495 pKa = 11.84SHH497 pKa = 5.71GWTTPSKK504 pKa = 10.76GIIGGARR511 pKa = 11.84FVRR514 pKa = 11.84RR515 pKa = 11.84GYY517 pKa = 10.18ISQGQNTLYY526 pKa = 10.78RR527 pKa = 11.84MRR529 pKa = 11.84WNPKK533 pKa = 9.65HH534 pKa = 6.64PGTHH538 pKa = 5.68QYY540 pKa = 9.23ATDD543 pKa = 3.61VRR545 pKa = 11.84WAQVQATTIKK555 pKa = 10.49SLYY558 pKa = 7.82EE559 pKa = 3.58QIGIKK564 pKa = 10.4GEE566 pKa = 3.79YY567 pKa = 9.66FIRR570 pKa = 11.84DD571 pKa = 3.27RR572 pKa = 11.84YY573 pKa = 9.4KK574 pKa = 10.69

Molecular weight: 65.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13645

38

1302

203.7

23.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.738 ± 0.808

0.652 ± 0.109

6.259 ± 0.35

7.131 ± 0.476

4.295 ± 0.201

5.694 ± 0.356

1.979 ± 0.228

7.534 ± 0.32

9.154 ± 0.474

7.453 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.182

7.028 ± 0.394

2.682 ± 0.205

3.694 ± 0.194

4.075 ± 0.326

6.207 ± 0.309

6.083 ± 0.332

6.31 ± 0.375

1.217 ± 0.151

4.39 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski