Streptococcus phage Javan472

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B632|A0A4D6B632_9CAUD Uncharacterized protein OS=Streptococcus phage Javan472 OX=2548191 GN=Javan472_0023 PE=4 SV=1
MM1 pKa = 7.64NYY3 pKa = 10.44LEE5 pKa = 4.33YY6 pKa = 11.13ALAYY10 pKa = 10.27LEE12 pKa = 4.77RR13 pKa = 11.84EE14 pKa = 4.39LEE16 pKa = 4.5IIDD19 pKa = 3.82NDD21 pKa = 3.93VIEE24 pKa = 4.46VEE26 pKa = 4.47LPNGGWEE33 pKa = 4.17FAPNPYY39 pKa = 9.89YY40 pKa = 10.49EE41 pKa = 5.01EE42 pKa = 4.65GLHH45 pKa = 6.64DD46 pKa = 3.79SPYY49 pKa = 10.48YY50 pKa = 10.02RR51 pKa = 11.84SQVAKK56 pKa = 10.55DD57 pKa = 3.09ILDD60 pKa = 3.46IKK62 pKa = 10.8GLLGRR67 pKa = 4.72

Molecular weight: 7.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B567|A0A4D6B567_9CAUD DNA primase OS=Streptococcus phage Javan472 OX=2548191 GN=Javan472_0018 PE=4 SV=1
MM1 pKa = 7.32SLIYY5 pKa = 10.37RR6 pKa = 11.84MRR8 pKa = 11.84GLDD11 pKa = 3.02RR12 pKa = 11.84FLRR15 pKa = 11.84SVEE18 pKa = 4.05RR19 pKa = 11.84KK20 pKa = 8.65QKK22 pKa = 9.38SVRR25 pKa = 11.84IAVDD29 pKa = 3.31KK30 pKa = 10.73EE31 pKa = 4.09LSKK34 pKa = 10.9SAARR38 pKa = 11.84IEE40 pKa = 4.15RR41 pKa = 11.84QAKK44 pKa = 9.19ILAPVDD50 pKa = 3.94TGWLRR55 pKa = 11.84AQIYY59 pKa = 10.15SEE61 pKa = 4.1QQRR64 pKa = 11.84LLHH67 pKa = 5.57YY68 pKa = 9.97RR69 pKa = 11.84VVSPALYY76 pKa = 10.27SIYY79 pKa = 10.79LEE81 pKa = 4.28LGTRR85 pKa = 11.84KK86 pKa = 8.85MEE88 pKa = 4.3AQSFLDD94 pKa = 3.47PALRR98 pKa = 11.84KK99 pKa = 8.48EE100 pKa = 4.46WPVLMANIKK109 pKa = 10.68KK110 pKa = 8.93MFKK113 pKa = 10.27RR114 pKa = 3.68

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12481

38

1460

235.5

26.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.036 ± 0.646

0.441 ± 0.092

6.049 ± 0.457

7.187 ± 0.555

3.734 ± 0.224

6.338 ± 0.377

1.194 ± 0.148

7.011 ± 0.341

8.629 ± 0.481

9.102 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.176

5.456 ± 0.256

2.756 ± 0.186

3.878 ± 0.273

4.222 ± 0.337

6.506 ± 0.676

6.258 ± 0.279

5.969 ± 0.21

1.01 ± 0.114

3.87 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski