Amino acid dipepetide frequency for Burkholderiales bacterium PBB4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.256AlaAla: 15.256 ± 0.155	1.31AlaCys: 1.31 ± 0.033	6.094AlaAsp: 6.094 ± 0.07	5.977AlaGlu: 5.977 ± 0.081	3.992AlaPhe: 3.992 ± 0.05	9.248AlaGly: 9.248 ± 0.092	2.763AlaHis: 2.763 ± 0.057	5.249AlaIle: 5.249 ± 0.065	4.765AlaLys: 4.765 ± 0.073	14.348AlaLeu: 14.348 ± 0.11	3.431AlaMet: 3.431 ± 0.056	3.319AlaAsn: 3.319 ± 0.059	5.695AlaPro: 5.695 ± 0.078	6.086AlaGln: 6.086 ± 0.08	7.118AlaArg: 7.118 ± 0.077	7.198AlaSer: 7.198 ± 0.085	6.43AlaThr: 6.43 ± 0.08	8.579AlaVal: 8.579 ± 0.082	1.94AlaTrp: 1.94 ± 0.045	2.539AlaTyr: 2.539 ± 0.046	0.002AlaXaa: 0.002 ± 0.001
Cys	1.144CysAla: 1.144 ± 0.033	0.13CysCys: 0.13 ± 0.011	0.5CysAsp: 0.5 ± 0.018	0.508CysGlu: 0.508 ± 0.02	0.333CysPhe: 0.333 ± 0.016	0.979CysGly: 0.979 ± 0.03	0.263CysHis: 0.263 ± 0.016	0.453CysIle: 0.453 ± 0.018	0.318CysLys: 0.318 ± 0.015	0.928CysLeu: 0.928 ± 0.027	0.225CysMet: 0.225 ± 0.013	0.289CysAsn: 0.289 ± 0.013	0.476CysPro: 0.476 ± 0.022	0.315CysGln: 0.315 ± 0.016	0.541CysArg: 0.541 ± 0.021	0.583CysSer: 0.583 ± 0.02	0.537CysThr: 0.537 ± 0.021	0.776CysVal: 0.776 ± 0.024	0.146CysTrp: 0.146 ± 0.018	0.201CysTyr: 0.201 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	6.585AspAla: 6.585 ± 0.064	0.506AspCys: 0.506 ± 0.017	2.482AspAsp: 2.482 ± 0.043	2.71AspGlu: 2.71 ± 0.043	2.128AspPhe: 2.128 ± 0.045	4.193AspGly: 4.193 ± 0.059	1.142AspHis: 1.142 ± 0.03	2.6AspIle: 2.6 ± 0.04	1.964AspLys: 1.964 ± 0.05	5.487AspLeu: 5.487 ± 0.065	1.265AspMet: 1.265 ± 0.031	1.293AspAsn: 1.293 ± 0.032	2.664AspPro: 2.664 ± 0.048	1.826AspGln: 1.826 ± 0.037	2.901AspArg: 2.901 ± 0.059	2.67AspSer: 2.67 ± 0.042	2.681AspThr: 2.681 ± 0.049	3.811AspVal: 3.811 ± 0.058	0.97AspTrp: 0.97 ± 0.027	1.417AspTyr: 1.417 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	6.221GluAla: 6.221 ± 0.074	0.374GluCys: 0.374 ± 0.016	2.218GluAsp: 2.218 ± 0.043	2.318GluGlu: 2.318 ± 0.047	1.926GluPhe: 1.926 ± 0.035	3.535GluGly: 3.535 ± 0.052	1.154GluHis: 1.154 ± 0.03	2.663GluIle: 2.663 ± 0.044	2.172GluLys: 2.172 ± 0.048	5.659GluLeu: 5.659 ± 0.066	1.272GluMet: 1.272 ± 0.03	1.337GluAsn: 1.337 ± 0.03	2.199GluPro: 2.199 ± 0.044	2.347GluGln: 2.347 ± 0.045	3.82GluArg: 3.82 ± 0.066	2.856GluSer: 2.856 ± 0.047	2.438GluThr: 2.438 ± 0.044	4.045GluVal: 4.045 ± 0.065	0.704GluTrp: 0.704 ± 0.021	1.036GluTyr: 1.036 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.361PheAla: 4.361 ± 0.047	0.423PheCys: 0.423 ± 0.018	2.506PheAsp: 2.506 ± 0.043	2.081PheGlu: 2.081 ± 0.045	1.426PhePhe: 1.426 ± 0.033	3.255PheGly: 3.255 ± 0.054	0.715PheHis: 0.715 ± 0.023	1.506PheIle: 1.506 ± 0.033	1.556PheLys: 1.556 ± 0.033	3.146PheLeu: 3.146 ± 0.056	0.83PheMet: 0.83 ± 0.026	1.29PheAsn: 1.29 ± 0.034	1.45PhePro: 1.45 ± 0.035	1.232PheGln: 1.232 ± 0.027	1.728PheArg: 1.728 ± 0.035	2.48PheSer: 2.48 ± 0.041	2.076PheThr: 2.076 ± 0.036	2.811PheVal: 2.811 ± 0.048	0.561PheTrp: 0.561 ± 0.022	0.923PheTyr: 0.923 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	8.64GlyAla: 8.64 ± 0.088	0.884GlyCys: 0.884 ± 0.026	3.925GlyAsp: 3.925 ± 0.057	3.757GlyGlu: 3.757 ± 0.057	3.257GlyPhe: 3.257 ± 0.051	6.29GlyGly: 6.29 ± 0.094	1.847GlyHis: 1.847 ± 0.038	3.961GlyIle: 3.961 ± 0.06	3.723GlyLys: 3.723 ± 0.064	8.686GlyLeu: 8.686 ± 0.087	2.185GlyMet: 2.185 ± 0.046	2.428GlyAsn: 2.428 ± 0.049	2.932GlyPro: 2.932 ± 0.052	3.474GlyGln: 3.474 ± 0.056	4.542GlyArg: 4.542 ± 0.056	4.776GlySer: 4.776 ± 0.064	4.636GlyThr: 4.636 ± 0.076	6.414GlyVal: 6.414 ± 0.081	1.341GlyTrp: 1.341 ± 0.035	2.312GlyTyr: 2.312 ± 0.047	0.002GlyXaa: 0.002 ± 0.001
His	2.698HisAla: 2.698 ± 0.052	0.314HisCys: 0.314 ± 0.015	1.076HisAsp: 1.076 ± 0.029	1.007HisGlu: 1.007 ± 0.028	0.955HisPhe: 0.955 ± 0.029	1.89HisGly: 1.89 ± 0.038	0.716HisHis: 0.716 ± 0.022	1.068HisIle: 1.068 ± 0.029	0.667HisLys: 0.667 ± 0.02	2.323HisLeu: 2.323 ± 0.05	0.532HisMet: 0.532 ± 0.019	0.54HisAsn: 0.54 ± 0.02	1.388HisPro: 1.388 ± 0.032	0.888HisGln: 0.888 ± 0.023	1.395HisArg: 1.395 ± 0.036	1.253HisSer: 1.253 ± 0.03	1.277HisThr: 1.277 ± 0.034	1.472HisVal: 1.472 ± 0.037	0.432HisTrp: 0.432 ± 0.017	0.689HisTyr: 0.689 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.123IleAla: 6.123 ± 0.066	0.424IleCys: 0.424 ± 0.016	2.835IleAsp: 2.835 ± 0.043	2.879IleGlu: 2.879 ± 0.044	1.437IlePhe: 1.437 ± 0.036	3.872IleGly: 3.872 ± 0.06	0.913IleHis: 0.913 ± 0.027	1.566IleIle: 1.566 ± 0.04	1.699IleLys: 1.699 ± 0.034	3.6IleLeu: 3.6 ± 0.06	0.759IleMet: 0.759 ± 0.024	1.495IleAsn: 1.495 ± 0.033	2.081IlePro: 2.081 ± 0.036	1.597IleGln: 1.597 ± 0.032	2.556IleArg: 2.556 ± 0.044	2.856IleSer: 2.856 ± 0.046	2.667IleThr: 2.667 ± 0.049	3.184IleVal: 3.184 ± 0.049	0.494IleTrp: 0.494 ± 0.022	1.007IleTyr: 1.007 ± 0.027	0.0IleXaa: 0.0 ± 0.0
Lys	4.703LysAla: 4.703 ± 0.067	0.219LysCys: 0.219 ± 0.015	2.039LysAsp: 2.039 ± 0.042	1.813LysGlu: 1.813 ± 0.04	1.279LysPhe: 1.279 ± 0.035	2.868LysGly: 2.868 ± 0.049	0.801LysHis: 0.801 ± 0.023	1.723LysIle: 1.723 ± 0.041	1.771LysLys: 1.771 ± 0.044	4.166LysLeu: 4.166 ± 0.065	0.934LysMet: 0.934 ± 0.023	1.19LysAsn: 1.19 ± 0.029	2.346LysPro: 2.346 ± 0.054	1.385LysGln: 1.385 ± 0.034	2.415LysArg: 2.415 ± 0.042	2.338LysSer: 2.338 ± 0.043	2.385LysThr: 2.385 ± 0.047	3.058LysVal: 3.058 ± 0.051	0.416LysTrp: 0.416 ± 0.019	0.783LysTyr: 0.783 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	13.58LeuAla: 13.58 ± 0.111	1.06LeuCys: 1.06 ± 0.027	5.594LeuAsp: 5.594 ± 0.067	5.41LeuGlu: 5.41 ± 0.081	3.37LeuPhe: 3.37 ± 0.048	8.675LeuGly: 8.675 ± 0.086	2.417LeuHis: 2.417 ± 0.049	4.2LeuIle: 4.2 ± 0.066	4.287LeuLys: 4.287 ± 0.06	10.707LeuLeu: 10.707 ± 0.122	2.709LeuMet: 2.709 ± 0.043	3.218LeuAsn: 3.218 ± 0.051	5.823LeuPro: 5.823 ± 0.061	5.012LeuGln: 5.012 ± 0.064	6.853LeuArg: 6.853 ± 0.07	6.686LeuSer: 6.686 ± 0.074	5.821LeuThr: 5.821 ± 0.068	8.088LeuVal: 8.088 ± 0.09	1.427LeuTrp: 1.427 ± 0.038	1.997LeuTyr: 1.997 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	3.186MetAla: 3.186 ± 0.055	0.19MetCys: 0.19 ± 0.012	1.246MetAsp: 1.246 ± 0.028	1.228MetGlu: 1.228 ± 0.032	0.747MetPhe: 0.747 ± 0.025	2.094MetGly: 2.094 ± 0.041	0.548MetHis: 0.548 ± 0.019	0.845MetIle: 0.845 ± 0.028	1.073MetLys: 1.073 ± 0.03	2.651MetLeu: 2.651 ± 0.047	0.577MetMet: 0.577 ± 0.023	0.847MetAsn: 0.847 ± 0.024	1.509MetPro: 1.509 ± 0.033	1.183MetGln: 1.183 ± 0.03	1.622MetArg: 1.622 ± 0.033	1.53MetSer: 1.53 ± 0.038	1.551MetThr: 1.551 ± 0.038	1.972MetVal: 1.972 ± 0.041	0.264MetTrp: 0.264 ± 0.014	0.428MetTyr: 0.428 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.578AsnAla: 3.578 ± 0.057	0.277AsnCys: 0.277 ± 0.014	1.522AsnAsp: 1.522 ± 0.037	1.268AsnGlu: 1.268 ± 0.035	1.142AsnPhe: 1.142 ± 0.03	2.421AsnGly: 2.421 ± 0.048	0.599AsnHis: 0.599 ± 0.021	1.38AsnIle: 1.38 ± 0.033	0.948AsnLys: 0.948 ± 0.031	3.074AsnLeu: 3.074 ± 0.051	0.632AsnMet: 0.632 ± 0.02	0.912AsnAsn: 0.912 ± 0.03	1.898AsnPro: 1.898 ± 0.041	1.102AsnGln: 1.102 ± 0.026	1.75AsnArg: 1.75 ± 0.035	1.638AsnSer: 1.638 ± 0.041	1.756AsnThr: 1.756 ± 0.04	2.118AsnVal: 2.118 ± 0.048	0.446AsnTrp: 0.446 ± 0.018	0.815AsnTyr: 0.815 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	6.162ProAla: 6.162 ± 0.085	0.349ProCys: 0.349 ± 0.015	2.916ProAsp: 2.916 ± 0.045	2.992ProGlu: 2.992 ± 0.048	1.761ProPhe: 1.761 ± 0.038	4.133ProGly: 4.133 ± 0.055	1.136ProHis: 1.136 ± 0.029	1.933ProIle: 1.933 ± 0.04	1.892ProLys: 1.892 ± 0.043	5.062ProLeu: 5.062 ± 0.064	1.375ProMet: 1.375 ± 0.03	1.309ProAsn: 1.309 ± 0.034	2.504ProPro: 2.504 ± 0.055	2.184ProGln: 2.184 ± 0.043	2.43ProArg: 2.43 ± 0.047	3.0ProSer: 3.0 ± 0.049	2.797ProThr: 2.797 ± 0.049	4.122ProVal: 4.122 ± 0.062	0.858ProTrp: 0.858 ± 0.029	1.076ProTyr: 1.076 ± 0.029	0.001ProXaa: 0.001 ± 0.001
Gln	5.748GlnAla: 5.748 ± 0.082	0.37GlnCys: 0.37 ± 0.02	1.847GlnAsp: 1.847 ± 0.039	1.853GlnGlu: 1.853 ± 0.047	1.467GlnPhe: 1.467 ± 0.033	3.373GlnGly: 3.373 ± 0.05	0.99GlnHis: 0.99 ± 0.028	1.878GlnIle: 1.878 ± 0.038	1.359GlnLys: 1.359 ± 0.03	4.56GlnLeu: 4.56 ± 0.06	1.097GlnMet: 1.097 ± 0.031	1.064GlnAsn: 1.064 ± 0.028	2.065GlnPro: 2.065 ± 0.037	1.966GlnGln: 1.966 ± 0.042	3.29GlnArg: 3.29 ± 0.051	2.688GlnSer: 2.688 ± 0.041	2.242GlnThr: 2.242 ± 0.037	3.329GlnVal: 3.329 ± 0.053	0.825GlnTrp: 0.825 ± 0.025	0.878GlnTyr: 0.878 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	6.582ArgAla: 6.582 ± 0.068	0.562ArgCys: 0.562 ± 0.024	3.133ArgAsp: 3.133 ± 0.051	3.338ArgGlu: 3.338 ± 0.055	2.509ArgPhe: 2.509 ± 0.043	3.909ArgGly: 3.909 ± 0.06	1.477ArgHis: 1.477 ± 0.033	3.144ArgIle: 3.144 ± 0.049	2.438ArgLys: 2.438 ± 0.046	6.833ArgLeu: 6.833 ± 0.083	1.768ArgMet: 1.768 ± 0.041	1.902ArgAsn: 1.902 ± 0.034	2.593ArgPro: 2.593 ± 0.041	2.66ArgGln: 2.66 ± 0.049	3.764ArgArg: 3.764 ± 0.051	3.591ArgSer: 3.591 ± 0.056	3.105ArgThr: 3.105 ± 0.045	4.534ArgVal: 4.534 ± 0.058	1.055ArgTrp: 1.055 ± 0.026	1.644ArgTyr: 1.644 ± 0.033	0.002ArgXaa: 0.002 ± 0.001
Ser	7.073SerAla: 7.073 ± 0.078	0.501SerCys: 0.501 ± 0.024	2.924SerAsp: 2.924 ± 0.044	2.837SerGlu: 2.837 ± 0.047	2.37SerPhe: 2.37 ± 0.045	5.551SerGly: 5.551 ± 0.078	1.325SerHis: 1.325 ± 0.031	2.788SerIle: 2.788 ± 0.043	2.199SerLys: 2.199 ± 0.041	6.426SerLeu: 6.426 ± 0.062	1.582SerMet: 1.582 ± 0.035	1.83SerAsn: 1.83 ± 0.044	3.082SerPro: 3.082 ± 0.052	2.34SerGln: 2.34 ± 0.046	3.414SerArg: 3.414 ± 0.057	3.728SerSer: 3.728 ± 0.068	3.536SerThr: 3.536 ± 0.054	4.4SerVal: 4.4 ± 0.065	0.821SerTrp: 0.821 ± 0.025	1.393SerTyr: 1.393 ± 0.039	0.001SerXaa: 0.001 ± 0.001
Thr	6.388ThrAla: 6.388 ± 0.076	0.463ThrCys: 0.463 ± 0.021	2.656ThrAsp: 2.656 ± 0.048	2.631ThrGlu: 2.631 ± 0.051	1.825ThrPhe: 1.825 ± 0.037	4.789ThrGly: 4.789 ± 0.076	1.299ThrHis: 1.299 ± 0.032	2.25ThrIle: 2.25 ± 0.042	1.722ThrLys: 1.722 ± 0.033	6.583ThrLeu: 6.583 ± 0.081	1.215ThrMet: 1.215 ± 0.031	1.509ThrAsn: 1.509 ± 0.046	3.701ThrPro: 3.701 ± 0.058	2.312ThrGln: 2.312 ± 0.038	3.081ThrArg: 3.081 ± 0.051	3.279ThrSer: 3.279 ± 0.064	3.32ThrThr: 3.32 ± 0.066	4.54ThrVal: 4.54 ± 0.055	0.802ThrTrp: 0.802 ± 0.023	1.225ThrTyr: 1.225 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	9.146ValAla: 9.146 ± 0.083	0.805ValCys: 0.805 ± 0.022	3.975ValAsp: 3.975 ± 0.05	3.999ValGlu: 3.999 ± 0.055	2.827ValPhe: 2.827 ± 0.052	5.815ValGly: 5.815 ± 0.074	1.661ValHis: 1.661 ± 0.038	3.355ValIle: 3.355 ± 0.051	2.747ValLys: 2.747 ± 0.05	8.19ValLeu: 8.19 ± 0.086	2.007ValMet: 2.007 ± 0.037	2.355ValAsn: 2.355 ± 0.04	3.742ValPro: 3.742 ± 0.051	3.208ValGln: 3.208 ± 0.051	4.685ValArg: 4.685 ± 0.066	4.731ValSer: 4.731 ± 0.054	4.177ValThr: 4.177 ± 0.06	6.724ValVal: 6.724 ± 0.09	1.152ValTrp: 1.152 ± 0.03	1.599ValTyr: 1.599 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.503TrpAla: 1.503 ± 0.034	0.201TrpCys: 0.201 ± 0.012	0.687TrpAsp: 0.687 ± 0.021	0.626TrpGlu: 0.626 ± 0.021	0.559TrpPhe: 0.559 ± 0.02	1.185TrpGly: 1.185 ± 0.035	0.366TrpHis: 0.366 ± 0.015	0.668TrpIle: 0.668 ± 0.026	0.536TrpLys: 0.536 ± 0.021	1.976TrpLeu: 1.976 ± 0.044	0.459TrpMet: 0.459 ± 0.019	0.506TrpAsn: 0.506 ± 0.019	0.691TrpPro: 0.691 ± 0.022	0.774TrpGln: 0.774 ± 0.025	1.102TrpArg: 1.102 ± 0.034	0.897TrpSer: 0.897 ± 0.027	0.802TrpThr: 0.802 ± 0.025	1.236TrpVal: 1.236 ± 0.03	0.313TrpTrp: 0.313 ± 0.016	0.297TrpTyr: 0.297 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.66TyrAla: 2.66 ± 0.046	0.282TyrCys: 0.282 ± 0.013	1.123TyrAsp: 1.123 ± 0.027	1.062TyrGlu: 1.062 ± 0.032	1.042TyrPhe: 1.042 ± 0.028	1.935TyrGly: 1.935 ± 0.042	0.45TyrHis: 0.45 ± 0.019	0.814TyrIle: 0.814 ± 0.025	0.794TyrLys: 0.794 ± 0.024	2.527TyrLeu: 2.527 ± 0.042	0.437TyrMet: 0.437 ± 0.02	0.642TyrAsn: 0.642 ± 0.021	1.155TyrPro: 1.155 ± 0.029	0.997TyrGln: 0.997 ± 0.03	1.538TyrArg: 1.538 ± 0.035	1.343TyrSer: 1.343 ± 0.033	1.356TyrThr: 1.356 ± 0.034	1.686TyrVal: 1.686 ± 0.042	0.416TyrTrp: 0.416 ± 0.018	0.595TyrTyr: 0.595 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.002XaaSer: 0.002 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.017XaaXaa: 0.017 ± 0.005

Statistics based on 5338 proteins (1380797 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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