Gordonia phage Gustav

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gustavvirus; Gordonia virus Gustav

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2H4PA60|A0A2H4PA60_9CAUD Uncharacterized protein OS=Gordonia phage Gustav OX=2047872 GN=PHIRE_GUSTAV_56 PE=4 SV=1
MM1 pKa = 7.81SDD3 pKa = 3.11TVVTIHH9 pKa = 5.86YY10 pKa = 8.06QSPADD15 pKa = 4.43DD16 pKa = 4.18PFKK19 pKa = 10.97VPRR22 pKa = 11.84PTRR25 pKa = 11.84IQFDD29 pKa = 3.35AAGNIQTTGGEE40 pKa = 4.38GEE42 pKa = 4.06ILGDD46 pKa = 3.58QLIGLSRR53 pKa = 11.84QDD55 pKa = 3.8GPPEE59 pKa = 4.26SFDD62 pKa = 3.37AEE64 pKa = 3.99NWVEE68 pKa = 4.0AEE70 pKa = 4.48EE71 pKa = 4.11ILSEE75 pKa = 4.25GVADD79 pKa = 5.19LEE81 pKa = 4.38LAGWFPAFMSGEE93 pKa = 4.41GIYY96 pKa = 8.72TWSGAVDD103 pKa = 3.4RR104 pKa = 11.84VEE106 pKa = 4.18VTVQQ110 pKa = 2.76

Molecular weight:
11.93 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2H4PA26|A0A2H4PA26_9CAUD Uncharacterized protein OS=Gordonia phage Gustav OX=2047872 GN=PHIRE_GUSTAV_28 PE=4 SV=1
MM1 pKa = 7.35EE2 pKa = 6.22ADD4 pKa = 3.78LSAYY8 pKa = 9.67HH9 pKa = 6.82HH10 pKa = 7.26LDD12 pKa = 3.08LRR14 pKa = 11.84DD15 pKa = 3.02RR16 pKa = 11.84WRR18 pKa = 11.84YY19 pKa = 10.13EE20 pKa = 3.86EE21 pKa = 4.24VLLASGHH28 pKa = 5.24RR29 pKa = 11.84VVLRR33 pKa = 11.84KK34 pKa = 8.68LTTRR38 pKa = 11.84MIYY41 pKa = 10.02VRR43 pKa = 11.84IRR45 pKa = 11.84HH46 pKa = 5.61GLPPEE51 pKa = 4.15SALAIHH57 pKa = 6.59FNGGKK62 pKa = 9.02WPWKK66 pKa = 10.61LGDD69 pKa = 4.31HH70 pKa = 6.89LLADD74 pKa = 4.93LYY76 pKa = 10.91FLHH79 pKa = 6.7RR80 pKa = 11.84QEE82 pKa = 5.91LEE84 pKa = 3.81GKK86 pKa = 9.0KK87 pKa = 10.68AKK89 pKa = 10.09DD90 pKa = 3.14HH91 pKa = 6.87PGRR94 pKa = 11.84PRR96 pKa = 11.84PDD98 pKa = 3.0KK99 pKa = 10.7TSTVSAARR107 pKa = 11.84ARR109 pKa = 11.84KK110 pKa = 8.46MRR112 pKa = 11.84DD113 pKa = 2.75ARR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84AAAEE121 pKa = 3.64EE122 pKa = 3.53ARR124 pKa = 11.84RR125 pKa = 11.84NYY127 pKa = 9.92RR128 pKa = 11.84RR129 pKa = 11.84SQGGGG134 pKa = 3.02

Molecular weight:
15.6 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

14241

33

1495

206.4

22.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.211 ± 0.478

0.59 ± 0.092

6.509 ± 0.274

6.039 ± 0.334

2.472 ± 0.146

9.016 ± 0.443

1.952 ± 0.151

4.108 ± 0.145

3.68 ± 0.211

8.153 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.149

2.97 ± 0.234

6.046 ± 0.313

3.265 ± 0.16

6.727 ± 0.383

5.091 ± 0.21

7.078 ± 0.277

7.436 ± 0.212

2.086 ± 0.126

2.38 ± 0.139

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski