Streptomyces phage Zuko

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6D728|A0A5J6D728_9CAUD Tail assembly chaperone OS=Streptomyces phage Zuko OX=2601695 GN=21 PE=4 SV=1
MM1 pKa = 7.83PYY3 pKa = 10.19EE4 pKa = 3.93IEE6 pKa = 3.9NPKK9 pKa = 10.18RR10 pKa = 11.84VEE12 pKa = 4.13IIIATAKK19 pKa = 10.27IVGFPVDD26 pKa = 3.35QLGALVLEE34 pKa = 5.02ALDD37 pKa = 4.57ADD39 pKa = 4.41HH40 pKa = 7.26DD41 pKa = 4.03QSLFPIYY48 pKa = 10.48NVLPDD53 pKa = 4.77AEE55 pKa = 4.42NADD58 pKa = 3.54PRR60 pKa = 11.84AFMIFFLHH68 pKa = 6.41NMIGSDD74 pKa = 4.43DD75 pKa = 3.67DD76 pKa = 3.91HH77 pKa = 9.39DD78 pKa = 5.29YY79 pKa = 11.14ISHH82 pKa = 7.36DD83 pKa = 4.39LDD85 pKa = 3.92HH86 pKa = 6.88ATNALDD92 pKa = 3.62PCPEE96 pKa = 4.62HH97 pKa = 6.38GTEE100 pKa = 5.99CSTCCDD106 pKa = 3.57EE107 pKa = 5.19CLSDD111 pKa = 4.06

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6D6Y9|A0A5J6D6Y9_9CAUD Tape measure protein OS=Streptomyces phage Zuko OX=2601695 GN=22 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84VYY4 pKa = 10.66SRR6 pKa = 11.84SRR8 pKa = 11.84LPISPDD14 pKa = 2.66SHH16 pKa = 7.49QEE18 pKa = 3.87LLIMPSQKK26 pKa = 9.02TVYY29 pKa = 9.77GPCVCCGRR37 pKa = 11.84MGYY40 pKa = 9.95HH41 pKa = 6.47EE42 pKa = 4.82ARR44 pKa = 11.84GLRR47 pKa = 11.84LACYY51 pKa = 10.12SRR53 pKa = 11.84WKK55 pKa = 10.52RR56 pKa = 11.84RR57 pKa = 11.84GLALKK62 pKa = 10.2RR63 pKa = 11.84FPTVRR68 pKa = 11.84RR69 pKa = 11.84KK70 pKa = 10.4GSYY73 pKa = 10.88DD74 pKa = 3.37LVKK77 pKa = 10.18PAAIRR82 pKa = 11.84QAWQYY87 pKa = 11.45AEE89 pKa = 4.75LVTGRR94 pKa = 11.84DD95 pKa = 3.0ACSRR99 pKa = 11.84EE100 pKa = 3.88RR101 pKa = 11.84ACIVLSISTRR111 pKa = 11.84TAIRR115 pKa = 11.84YY116 pKa = 7.37EE117 pKa = 4.13RR118 pKa = 11.84YY119 pKa = 10.38LGMKK123 pKa = 9.12YY124 pKa = 9.98RR125 pKa = 11.84KK126 pKa = 8.84QVGYY130 pKa = 10.93GRR132 pKa = 11.84GGEE135 pKa = 4.02

Molecular weight: 15.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

115

0

115

23721

29

1409

206.3

22.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.671 ± 0.367

1.172 ± 0.132

6.458 ± 0.201

6.69 ± 0.423

3.17 ± 0.115

7.786 ± 0.368

2.209 ± 0.162

4.342 ± 0.191

4.894 ± 0.3

7.382 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.14

3.529 ± 0.214

5.493 ± 0.245

3.469 ± 0.12

6.184 ± 0.233

5.78 ± 0.281

6.872 ± 0.348

7.044 ± 0.176

2.066 ± 0.126

2.976 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski