Amino acid dipepetide frequency for Ekhidna lutea

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.261AlaAla: 4.261 ± 0.077	0.561AlaCys: 0.561 ± 0.025	3.891AlaAsp: 3.891 ± 0.075	4.302AlaGlu: 4.302 ± 0.078	3.475AlaPhe: 3.475 ± 0.06	4.747AlaGly: 4.747 ± 0.095	1.078AlaHis: 1.078 ± 0.027	5.109AlaIle: 5.109 ± 0.071	3.91AlaLys: 3.91 ± 0.068	5.976AlaLeu: 5.976 ± 0.075	1.628AlaMet: 1.628 ± 0.037	3.269AlaAsn: 3.269 ± 0.064	1.904AlaPro: 1.904 ± 0.043	2.295AlaGln: 2.295 ± 0.051	2.28AlaArg: 2.28 ± 0.046	4.512AlaSer: 4.512 ± 0.078	3.6AlaThr: 3.6 ± 0.095	3.881AlaVal: 3.881 ± 0.063	0.669AlaTrp: 0.669 ± 0.022	2.438AlaTyr: 2.438 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.438CysAla: 0.438 ± 0.02	0.093CysCys: 0.093 ± 0.009	0.461CysAsp: 0.461 ± 0.034	0.498CysGlu: 0.498 ± 0.023	0.354CysPhe: 0.354 ± 0.019	0.574CysGly: 0.574 ± 0.023	0.147CysHis: 0.147 ± 0.012	0.516CysIle: 0.516 ± 0.022	0.341CysLys: 0.341 ± 0.017	0.601CysLeu: 0.601 ± 0.022	0.138CysMet: 0.138 ± 0.011	0.321CysAsn: 0.321 ± 0.018	0.286CysPro: 0.286 ± 0.016	0.233CysGln: 0.233 ± 0.014	0.231CysArg: 0.231 ± 0.013	0.499CysSer: 0.499 ± 0.023	0.401CysThr: 0.401 ± 0.02	0.393CysVal: 0.393 ± 0.019	0.075CysTrp: 0.075 ± 0.008	0.251CysTyr: 0.251 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.734AspAla: 3.734 ± 0.093	0.355AspCys: 0.355 ± 0.02	3.675AspAsp: 3.675 ± 0.083	4.743AspGlu: 4.743 ± 0.073	3.569AspPhe: 3.569 ± 0.062	5.062AspGly: 5.062 ± 0.181	1.145AspHis: 1.145 ± 0.037	4.334AspIle: 4.334 ± 0.069	3.448AspLys: 3.448 ± 0.061	5.881AspLeu: 5.881 ± 0.069	1.275AspMet: 1.275 ± 0.033	2.998AspAsn: 2.998 ± 0.076	2.544AspPro: 2.544 ± 0.05	2.408AspGln: 2.408 ± 0.047	2.432AspArg: 2.432 ± 0.049	3.914AspSer: 3.914 ± 0.083	2.773AspThr: 2.773 ± 0.068	3.8AspVal: 3.8 ± 0.069	0.752AspTrp: 0.752 ± 0.025	2.66AspTyr: 2.66 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.608GluAla: 4.608 ± 0.063	0.345GluCys: 0.345 ± 0.018	3.991GluAsp: 3.991 ± 0.066	5.714GluGlu: 5.714 ± 0.096	3.127GluPhe: 3.127 ± 0.057	4.383GluGly: 4.383 ± 0.071	1.103GluHis: 1.103 ± 0.031	5.661GluIle: 5.661 ± 0.074	5.487GluLys: 5.487 ± 0.091	6.713GluLeu: 6.713 ± 0.08	1.865GluMet: 1.865 ± 0.038	4.067GluAsn: 4.067 ± 0.063	1.865GluPro: 1.865 ± 0.047	2.388GluGln: 2.388 ± 0.052	2.847GluArg: 2.847 ± 0.045	4.249GluSer: 4.249 ± 0.063	3.482GluThr: 3.482 ± 0.058	5.108GluVal: 5.108 ± 0.076	0.86GluTrp: 0.86 ± 0.025	2.541GluTyr: 2.541 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.873PheAla: 2.873 ± 0.044	0.391PheCys: 0.391 ± 0.018	3.527PheAsp: 3.527 ± 0.059	3.658PheGlu: 3.658 ± 0.067	2.666PhePhe: 2.666 ± 0.048	3.581PheGly: 3.581 ± 0.05	0.921PheHis: 0.921 ± 0.027	3.768PheIle: 3.768 ± 0.058	2.997PheLys: 2.997 ± 0.061	4.527PheLeu: 4.527 ± 0.072	1.16PheMet: 1.16 ± 0.029	2.873PheAsn: 2.873 ± 0.056	1.601PhePro: 1.601 ± 0.035	1.724PheGln: 1.724 ± 0.035	1.923PheArg: 1.923 ± 0.042	3.967PheSer: 3.967 ± 0.068	3.167PheThr: 3.167 ± 0.059	2.917PheVal: 2.917 ± 0.053	0.603PheTrp: 0.603 ± 0.022	2.108PheTyr: 2.108 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.446GlyAla: 4.446 ± 0.095	0.587GlyCys: 0.587 ± 0.029	4.315GlyAsp: 4.315 ± 0.102	4.315GlyGlu: 4.315 ± 0.058	3.685GlyPhe: 3.685 ± 0.062	5.176GlyGly: 5.176 ± 0.097	1.092GlyHis: 1.092 ± 0.033	5.417GlyIle: 5.417 ± 0.077	4.399GlyLys: 4.399 ± 0.075	6.211GlyLeu: 6.211 ± 0.086	1.724GlyMet: 1.724 ± 0.035	3.801GlyAsn: 3.801 ± 0.092	1.532GlyPro: 1.532 ± 0.038	2.151GlyGln: 2.151 ± 0.05	2.529GlyArg: 2.529 ± 0.049	4.82GlySer: 4.82 ± 0.09	4.44GlyThr: 4.44 ± 0.119	4.722GlyVal: 4.722 ± 0.069	0.928GlyTrp: 0.928 ± 0.034	2.801GlyTyr: 2.801 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.032HisAla: 1.032 ± 0.029	0.158HisCys: 0.158 ± 0.012	0.944HisAsp: 0.944 ± 0.03	1.154HisGlu: 1.154 ± 0.035	1.121HisPhe: 1.121 ± 0.029	1.137HisGly: 1.137 ± 0.031	0.517HisHis: 0.517 ± 0.022	1.286HisIle: 1.286 ± 0.037	1.024HisLys: 1.024 ± 0.03	1.888HisLeu: 1.888 ± 0.04	0.356HisMet: 0.356 ± 0.017	0.746HisAsn: 0.746 ± 0.025	0.906HisPro: 0.906 ± 0.027	0.694HisGln: 0.694 ± 0.024	0.729HisArg: 0.729 ± 0.03	1.189HisSer: 1.189 ± 0.037	0.908HisThr: 0.908 ± 0.025	0.957HisVal: 0.957 ± 0.028	0.205HisTrp: 0.205 ± 0.013	0.798HisTyr: 0.798 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	5.36IleAla: 5.36 ± 0.078	0.549IleCys: 0.549 ± 0.019	5.019IleAsp: 5.019 ± 0.066	5.253IleGlu: 5.253 ± 0.077	3.432IlePhe: 3.432 ± 0.061	5.282IleGly: 5.282 ± 0.075	1.478IleHis: 1.478 ± 0.04	5.43IleIle: 5.43 ± 0.092	4.843IleLys: 4.843 ± 0.078	6.804IleLeu: 6.804 ± 0.093	1.362IleMet: 1.362 ± 0.032	4.229IleAsn: 4.229 ± 0.064	3.087IlePro: 3.087 ± 0.06	2.555IleGln: 2.555 ± 0.052	2.996IleArg: 2.996 ± 0.052	5.942IleSer: 5.942 ± 0.077	4.605IleThr: 4.605 ± 0.078	4.527IleVal: 4.527 ± 0.064	0.729IleTrp: 0.729 ± 0.027	2.729IleTyr: 2.729 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.292LysAla: 4.292 ± 0.072	0.333LysCys: 0.333 ± 0.016	3.875LysAsp: 3.875 ± 0.064	5.744LysGlu: 5.744 ± 0.091	2.398LysPhe: 2.398 ± 0.051	4.065LysGly: 4.065 ± 0.072	1.173LysHis: 1.173 ± 0.029	4.656LysIle: 4.656 ± 0.077	5.455LysLys: 5.455 ± 0.095	5.858LysLeu: 5.858 ± 0.101	1.758LysMet: 1.758 ± 0.044	3.597LysAsn: 3.597 ± 0.055	2.15LysPro: 2.15 ± 0.046	2.186LysGln: 2.186 ± 0.04	2.711LysArg: 2.711 ± 0.059	4.29LysSer: 4.29 ± 0.073	3.308LysThr: 3.308 ± 0.065	4.396LysVal: 4.396 ± 0.077	0.791LysTrp: 0.791 ± 0.027	2.581LysTyr: 2.581 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	6.071LeuAla: 6.071 ± 0.079	0.608LeuCys: 0.608 ± 0.023	5.548LeuAsp: 5.548 ± 0.084	6.23LeuGlu: 6.23 ± 0.09	4.583LeuPhe: 4.583 ± 0.064	5.904LeuGly: 5.904 ± 0.073	1.461LeuHis: 1.461 ± 0.034	7.58LeuIle: 7.58 ± 0.109	6.764LeuLys: 6.764 ± 0.107	8.405LeuLeu: 8.405 ± 0.124	2.139LeuMet: 2.139 ± 0.046	5.218LeuAsn: 5.218 ± 0.07	3.32LeuPro: 3.32 ± 0.054	2.907LeuGln: 2.907 ± 0.052	3.518LeuArg: 3.518 ± 0.057	7.356LeuSer: 7.356 ± 0.086	5.42LeuThr: 5.42 ± 0.078	5.719LeuVal: 5.719 ± 0.083	0.86LeuTrp: 0.86 ± 0.031	2.962LeuTyr: 2.962 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	1.573MetAla: 1.573 ± 0.035	0.133MetCys: 0.133 ± 0.011	1.386MetAsp: 1.386 ± 0.032	1.602MetGlu: 1.602 ± 0.037	0.82MetPhe: 0.82 ± 0.032	1.548MetGly: 1.548 ± 0.032	0.405MetHis: 0.405 ± 0.021	1.804MetIle: 1.804 ± 0.048	2.137MetLys: 2.137 ± 0.049	1.952MetLeu: 1.952 ± 0.042	0.668MetMet: 0.668 ± 0.022	1.326MetAsn: 1.326 ± 0.028	0.869MetPro: 0.869 ± 0.024	0.736MetGln: 0.736 ± 0.025	1.007MetArg: 1.007 ± 0.028	1.51MetSer: 1.51 ± 0.039	1.212MetThr: 1.212 ± 0.03	1.444MetVal: 1.444 ± 0.038	0.199MetTrp: 0.199 ± 0.013	0.668MetTyr: 0.668 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.487AsnAla: 3.487 ± 0.066	0.387AsnCys: 0.387 ± 0.021	3.293AsnAsp: 3.293 ± 0.073	3.836AsnGlu: 3.836 ± 0.052	2.572AsnPhe: 2.572 ± 0.041	4.001AsnGly: 4.001 ± 0.091	1.108AsnHis: 1.108 ± 0.032	3.734AsnIle: 3.734 ± 0.055	3.077AsnLys: 3.077 ± 0.052	5.023AsnLeu: 5.023 ± 0.065	1.183AsnMet: 1.183 ± 0.03	2.936AsnAsn: 2.936 ± 0.07	2.737AsnPro: 2.737 ± 0.048	2.249AsnGln: 2.249 ± 0.05	2.241AsnArg: 2.241 ± 0.038	3.597AsnSer: 3.597 ± 0.07	2.873AsnThr: 2.873 ± 0.084	3.211AsnVal: 3.211 ± 0.063	0.73AsnTrp: 0.73 ± 0.027	2.407AsnTyr: 2.407 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	2.161ProAla: 2.161 ± 0.048	0.192ProCys: 0.192 ± 0.013	2.564ProAsp: 2.564 ± 0.062	2.991ProGlu: 2.991 ± 0.052	1.862ProPhe: 1.862 ± 0.041	2.302ProGly: 2.302 ± 0.05	0.592ProHis: 0.592 ± 0.023	2.594ProIle: 2.594 ± 0.048	2.073ProLys: 2.073 ± 0.046	2.801ProLeu: 2.801 ± 0.053	0.753ProMet: 0.753 ± 0.026	2.082ProAsn: 2.082 ± 0.053	1.008ProPro: 1.008 ± 0.035	1.061ProGln: 1.061 ± 0.027	1.063ProArg: 1.063 ± 0.033	2.421ProSer: 2.421 ± 0.044	2.101ProThr: 2.101 ± 0.048	2.39ProVal: 2.39 ± 0.048	0.365ProTrp: 0.365 ± 0.02	1.321ProTyr: 1.321 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.19GlnAla: 2.19 ± 0.043	0.189GlnCys: 0.189 ± 0.012	1.768GlnAsp: 1.768 ± 0.037	2.344GlnGlu: 2.344 ± 0.05	1.785GlnPhe: 1.785 ± 0.04	1.909GlnGly: 1.909 ± 0.04	0.569GlnHis: 0.569 ± 0.022	2.7GlnIle: 2.7 ± 0.048	2.472GlnLys: 2.472 ± 0.042	3.45GlnLeu: 3.45 ± 0.058	0.852GlnMet: 0.852 ± 0.025	1.909GlnAsn: 1.909 ± 0.04	1.15GlnPro: 1.15 ± 0.027	1.31GlnGln: 1.31 ± 0.034	1.336GlnArg: 1.336 ± 0.03	2.371GlnSer: 2.371 ± 0.048	1.831GlnThr: 1.831 ± 0.044	2.168GlnVal: 2.168 ± 0.04	0.398GlnTrp: 0.398 ± 0.02	1.335GlnTyr: 1.335 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	2.204ArgAla: 2.204 ± 0.041	0.2ArgCys: 0.2 ± 0.012	2.066ArgAsp: 2.066 ± 0.042	2.589ArgGlu: 2.589 ± 0.051	2.094ArgPhe: 2.094 ± 0.042	2.147ArgGly: 2.147 ± 0.043	0.618ArgHis: 0.618 ± 0.022	3.191ArgIle: 3.191 ± 0.054	3.077ArgLys: 3.077 ± 0.064	3.659ArgLeu: 3.659 ± 0.062	1.076ArgMet: 1.076 ± 0.032	2.198ArgAsn: 2.198 ± 0.042	1.233ArgPro: 1.233 ± 0.032	1.238ArgGln: 1.238 ± 0.031	1.647ArgArg: 1.647 ± 0.039	2.543ArgSer: 2.543 ± 0.045	1.949ArgThr: 1.949 ± 0.04	2.525ArgVal: 2.525 ± 0.047	0.487ArgTrp: 0.487 ± 0.021	1.56ArgTyr: 1.56 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	4.377SerAla: 4.377 ± 0.074	0.557SerCys: 0.557 ± 0.023	4.265SerAsp: 4.265 ± 0.082	4.538SerGlu: 4.538 ± 0.058	4.142SerPhe: 4.142 ± 0.064	5.479SerGly: 5.479 ± 0.11	1.194SerHis: 1.194 ± 0.034	5.67SerIle: 5.67 ± 0.07	4.175SerLys: 4.175 ± 0.066	6.768SerLeu: 6.768 ± 0.08	1.511SerMet: 1.511 ± 0.034	3.811SerAsn: 3.811 ± 0.07	2.351SerPro: 2.351 ± 0.044	2.344SerGln: 2.344 ± 0.047	2.503SerArg: 2.503 ± 0.052	5.227SerSer: 5.227 ± 0.1	3.946SerThr: 3.946 ± 0.073	4.208SerVal: 4.208 ± 0.057	0.867SerTrp: 0.867 ± 0.033	2.949SerTyr: 2.949 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	3.503ThrAla: 3.503 ± 0.072	0.354ThrCys: 0.354 ± 0.019	3.544ThrAsp: 3.544 ± 0.11	3.316ThrGlu: 3.316 ± 0.054	3.063ThrPhe: 3.063 ± 0.057	4.2ThrGly: 4.2 ± 0.095	0.996ThrHis: 0.996 ± 0.028	4.8ThrIle: 4.8 ± 0.078	3.005ThrLys: 3.005 ± 0.05	5.211ThrLeu: 5.211 ± 0.081	0.983ThrMet: 0.983 ± 0.032	3.004ThrAsn: 3.004 ± 0.08	2.229ThrPro: 2.229 ± 0.052	1.693ThrGln: 1.693 ± 0.042	1.755ThrArg: 1.755 ± 0.044	4.055ThrSer: 4.055 ± 0.081	3.32ThrThr: 3.32 ± 0.087	3.764ThrVal: 3.764 ± 0.103	0.62ThrTrp: 0.62 ± 0.025	2.364ThrTyr: 2.364 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	4.192ValAla: 4.192 ± 0.067	0.477ValCys: 0.477 ± 0.022	4.09ValAsp: 4.09 ± 0.077	4.257ValGlu: 4.257 ± 0.06	3.245ValPhe: 3.245 ± 0.059	4.294ValGly: 4.294 ± 0.062	1.037ValHis: 1.037 ± 0.035	4.83ValIle: 4.83 ± 0.063	3.949ValLys: 3.949 ± 0.068	5.745ValLeu: 5.745 ± 0.078	1.467ValMet: 1.467 ± 0.037	3.565ValAsn: 3.565 ± 0.061	2.165ValPro: 2.165 ± 0.045	1.843ValGln: 1.843 ± 0.036	2.337ValArg: 2.337 ± 0.048	4.903ValSer: 4.903 ± 0.076	3.726ValThr: 3.726 ± 0.102	4.307ValVal: 4.307 ± 0.06	0.67ValTrp: 0.67 ± 0.022	2.26ValTyr: 2.26 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.63TrpAla: 0.63 ± 0.023	0.085TrpCys: 0.085 ± 0.009	0.698TrpAsp: 0.698 ± 0.023	0.717TrpGlu: 0.717 ± 0.027	0.578TrpPhe: 0.578 ± 0.024	0.732TrpGly: 0.732 ± 0.028	0.26TrpHis: 0.26 ± 0.015	0.879TrpIle: 0.879 ± 0.028	0.784TrpLys: 0.784 ± 0.029	1.112TrpLeu: 1.112 ± 0.035	0.349TrpMet: 0.349 ± 0.016	0.71TrpAsn: 0.71 ± 0.025	0.296TrpPro: 0.296 ± 0.016	0.39TrpGln: 0.39 ± 0.018	0.445TrpArg: 0.445 ± 0.02	0.77TrpSer: 0.77 ± 0.029	0.626TrpThr: 0.626 ± 0.028	0.737TrpVal: 0.737 ± 0.028	0.189TrpTrp: 0.189 ± 0.013	0.474TrpTyr: 0.474 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.354TyrAla: 2.354 ± 0.047	0.297TyrCys: 0.297 ± 0.015	2.58TyrAsp: 2.58 ± 0.051	2.606TyrGlu: 2.606 ± 0.05	2.379TyrPhe: 2.379 ± 0.045	2.568TyrGly: 2.568 ± 0.049	0.856TyrHis: 0.856 ± 0.029	2.252TyrIle: 2.252 ± 0.045	2.199TyrLys: 2.199 ± 0.048	3.988TyrLeu: 3.988 ± 0.066	0.752TyrMet: 0.752 ± 0.026	2.02TyrAsn: 2.02 ± 0.045	1.464TyrPro: 1.464 ± 0.034	1.632TyrGln: 1.632 ± 0.035	1.771TyrArg: 1.771 ± 0.043	2.748TyrSer: 2.748 ± 0.052	2.09TyrThr: 2.09 ± 0.045	2.189TyrVal: 2.189 ± 0.043	0.465TyrTrp: 0.465 ± 0.019	1.849TyrTyr: 1.849 ± 0.054	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3721 proteins (1309046 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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