Gordonia phage MintFen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514CXV5|A0A514CXV5_9CAUD Minor tail protein OS=Gordonia phage MintFen OX=2588491 GN=28 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.91NPWYY45 pKa = 10.09EE46 pKa = 3.62PTYY49 pKa = 11.08NYY51 pKa = 10.89GVDD54 pKa = 3.91PNGHH58 pKa = 5.62TEE60 pKa = 4.15VTDD63 pKa = 3.08PWKK66 pKa = 10.68RR67 pKa = 11.84HH68 pKa = 3.81TQFPEE73 pKa = 3.86VCAMGFGGPNNSGLPTEE90 pKa = 4.89PPPLPAEE97 pKa = 4.28SEE99 pKa = 4.11PTPDD103 pKa = 4.75LEE105 pKa = 4.91EE106 pKa = 4.43PTDD109 pKa = 3.7GG110 pKa = 4.88

Molecular weight: 11.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514CY03|A0A514CY03_9CAUD Uncharacterized protein OS=Gordonia phage MintFen OX=2588491 GN=65 PE=4 SV=1
MM1 pKa = 6.63TTLDD5 pKa = 3.6KK6 pKa = 11.34SRR8 pKa = 11.84ILNTQIEE15 pKa = 4.95VLCPHH20 pKa = 6.92GPAPLLIPPSRR31 pKa = 11.84RR32 pKa = 11.84GRR34 pKa = 11.84ARR36 pKa = 11.84TLDD39 pKa = 3.42TTHH42 pKa = 7.27PMLGWHH48 pKa = 5.65QPCGDD53 pKa = 3.57RR54 pKa = 11.84ASPHH58 pKa = 5.25RR59 pKa = 11.84TEE61 pKa = 4.65HH62 pKa = 6.58AAPAPRR68 pKa = 11.84RR69 pKa = 11.84APNRR73 pKa = 11.84HH74 pKa = 5.79EE75 pKa = 4.26NPMARR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84PNPEE86 pKa = 3.24QRR88 pKa = 11.84GLGHH92 pKa = 6.69AHH94 pKa = 6.24KK95 pKa = 10.55QQVKK99 pKa = 9.27HH100 pKa = 5.94LKK102 pKa = 7.95TRR104 pKa = 11.84HH105 pKa = 5.47VDD107 pKa = 3.6GTPCWWCGKK116 pKa = 9.42PMYY119 pKa = 10.3LDD121 pKa = 3.19RR122 pKa = 11.84TRR124 pKa = 11.84NPDD127 pKa = 3.69YY128 pKa = 11.07NPTSPDD134 pKa = 3.55PASGSLAGDD143 pKa = 3.23HH144 pKa = 6.01TLARR148 pKa = 11.84THH150 pKa = 6.66GGTLADD156 pKa = 3.84RR157 pKa = 11.84LLHH160 pKa = 5.94GRR162 pKa = 11.84CNKK165 pKa = 9.38QRR167 pKa = 11.84GDD169 pKa = 3.25GRR171 pKa = 11.84HH172 pKa = 5.79DD173 pKa = 3.32HH174 pKa = 6.33TRR176 pKa = 11.84PALTGKK182 pKa = 8.91HH183 pKa = 4.65QPAPTTHH190 pKa = 7.03LAINCWPP197 pKa = 3.68

Molecular weight: 22.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

15900

40

1840

201.3

21.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.711 ± 0.44

0.824 ± 0.14

6.881 ± 0.315

5.415 ± 0.356

2.811 ± 0.147

8.491 ± 0.32

2.113 ± 0.198

4.604 ± 0.184

3.421 ± 0.226

7.755 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.195

2.855 ± 0.143

5.925 ± 0.256

3.264 ± 0.139

7.447 ± 0.378

5.478 ± 0.213

6.623 ± 0.294

7.887 ± 0.213

2.019 ± 0.142

2.233 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski