Amino acid dipepetide frequency for Citrobacter phage CF1 ERZ-2017

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.37AlaAla: 5.37 ± 0.358
0.427AlaCys: 0.427 ± 0.089
4.032AlaAsp: 4.032 ± 0.261
5.277AlaGlu: 5.277 ± 0.402
2.285AlaPhe: 2.285 ± 0.227
4.18AlaGly: 4.18 ± 0.369
1.115AlaHis: 1.115 ± 0.129
5.277AlaIle: 5.277 ± 0.344
4.942AlaLys: 4.942 ± 0.298
6.057AlaLeu: 6.057 ± 0.341
1.839AlaMet: 1.839 ± 0.169
3.251AlaAsn: 3.251 ± 0.274
2.285AlaPro: 2.285 ± 0.221
2.564AlaGln: 2.564 ± 0.252
3.121AlaArg: 3.121 ± 0.245
3.92AlaSer: 3.92 ± 0.281
3.549AlaThr: 3.549 ± 0.407
4.608AlaVal: 4.608 ± 0.344
1.04AlaTrp: 1.04 ± 0.138
2.62AlaTyr: 2.62 ± 0.263
0.0AlaXaa: 0.0 ± 0.0
Cys
0.52CysAla: 0.52 ± 0.1
0.13CysCys: 0.13 ± 0.055
0.818CysAsp: 0.818 ± 0.13
0.818CysGlu: 0.818 ± 0.129
0.52CysPhe: 0.52 ± 0.114
0.855CysGly: 0.855 ± 0.123
0.39CysHis: 0.39 ± 0.08
0.632CysIle: 0.632 ± 0.116
0.873CysLys: 0.873 ± 0.152
0.706CysLeu: 0.706 ± 0.117
0.39CysMet: 0.39 ± 0.078
0.502CysAsn: 0.502 ± 0.103
0.502CysPro: 0.502 ± 0.102
0.39CysGln: 0.39 ± 0.082
0.576CysArg: 0.576 ± 0.103
0.929CysSer: 0.929 ± 0.124
0.576CysThr: 0.576 ± 0.091
0.483CysVal: 0.483 ± 0.111
0.223CysTrp: 0.223 ± 0.067
0.464CysTyr: 0.464 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
3.902AspAla: 3.902 ± 0.323
0.576AspCys: 0.576 ± 0.089
4.273AspAsp: 4.273 ± 0.282
4.664AspGlu: 4.664 ± 0.321
3.047AspPhe: 3.047 ± 0.233
4.329AspGly: 4.329 ± 0.343
1.096AspHis: 1.096 ± 0.137
4.775AspIle: 4.775 ± 0.284
4.385AspLys: 4.385 ± 0.279
5.184AspLeu: 5.184 ± 0.312
1.672AspMet: 1.672 ± 0.192
3.382AspAsn: 3.382 ± 0.221
2.192AspPro: 2.192 ± 0.203
1.951AspGln: 1.951 ± 0.195
2.806AspArg: 2.806 ± 0.223
4.069AspSer: 4.069 ± 0.271
3.27AspThr: 3.27 ± 0.255
4.05AspVal: 4.05 ± 0.238
1.319AspTrp: 1.319 ± 0.165
3.307AspTyr: 3.307 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
5.388GluAla: 5.388 ± 0.344
1.04GluCys: 1.04 ± 0.127
4.533GluAsp: 4.533 ± 0.302
5.332GluGlu: 5.332 ± 0.355
3.53GluPhe: 3.53 ± 0.279
3.827GluGly: 3.827 ± 0.254
1.412GluHis: 1.412 ± 0.162
5.741GluIle: 5.741 ± 0.378
5.332GluLys: 5.332 ± 0.287
7.246GluLeu: 7.246 ± 0.395
2.564GluMet: 2.564 ± 0.198
3.493GluAsn: 3.493 ± 0.236
1.486GluPro: 1.486 ± 0.172
2.267GluGln: 2.267 ± 0.238
2.713GluArg: 2.713 ± 0.235
4.162GluSer: 4.162 ± 0.268
3.716GluThr: 3.716 ± 0.261
5.091GluVal: 5.091 ± 0.277
0.966GluTrp: 0.966 ± 0.121
3.084GluTyr: 3.084 ± 0.287
0.0GluXaa: 0.0 ± 0.0
Phe
2.713PheAla: 2.713 ± 0.231
0.595PheCys: 0.595 ± 0.098
3.27PheAsp: 3.27 ± 0.24
3.604PheGlu: 3.604 ± 0.286
1.561PhePhe: 1.561 ± 0.173
3.103PheGly: 3.103 ± 0.241
0.799PheHis: 0.799 ± 0.114
3.177PheIle: 3.177 ± 0.241
3.716PheLys: 3.716 ± 0.264
2.285PheLeu: 2.285 ± 0.21
1.338PheMet: 1.338 ± 0.172
3.047PheAsn: 3.047 ± 0.263
1.133PhePro: 1.133 ± 0.129
1.616PheGln: 1.616 ± 0.198
1.877PheArg: 1.877 ± 0.182
2.657PheSer: 2.657 ± 0.22
2.267PheThr: 2.267 ± 0.203
2.806PheVal: 2.806 ± 0.225
0.613PheTrp: 0.613 ± 0.102
1.524PheTyr: 1.524 ± 0.198
0.0PheXaa: 0.0 ± 0.0
Gly
3.642GlyAla: 3.642 ± 0.282
0.632GlyCys: 0.632 ± 0.117
4.162GlyAsp: 4.162 ± 0.325
4.106GlyGlu: 4.106 ± 0.222
3.382GlyPhe: 3.382 ± 0.281
4.032GlyGly: 4.032 ± 0.442
1.078GlyHis: 1.078 ± 0.14
3.995GlyIle: 3.995 ± 0.33
4.311GlyLys: 4.311 ± 0.293
4.794GlyLeu: 4.794 ± 0.317
1.877GlyMet: 1.877 ± 0.201
2.824GlyAsn: 2.824 ± 0.304
1.616GlyPro: 1.616 ± 0.188
2.118GlyGln: 2.118 ± 0.218
2.768GlyArg: 2.768 ± 0.206
4.255GlySer: 4.255 ± 0.231
4.218GlyThr: 4.218 ± 0.354
4.366GlyVal: 4.366 ± 0.288
1.022GlyTrp: 1.022 ± 0.152
2.843GlyTyr: 2.843 ± 0.199
0.0GlyXaa: 0.0 ± 0.0
His
1.078HisAla: 1.078 ± 0.162
0.316HisCys: 0.316 ± 0.089
1.226HisAsp: 1.226 ± 0.152
1.245HisGlu: 1.245 ± 0.138
0.929HisPhe: 0.929 ± 0.139
1.356HisGly: 1.356 ± 0.15
0.39HisHis: 0.39 ± 0.101
1.356HisIle: 1.356 ± 0.179
1.412HisLys: 1.412 ± 0.182
1.654HisLeu: 1.654 ± 0.205
0.595HisMet: 0.595 ± 0.112
0.725HisAsn: 0.725 ± 0.107
0.78HisPro: 0.78 ± 0.124
0.632HisGln: 0.632 ± 0.089
0.725HisArg: 0.725 ± 0.115
1.059HisSer: 1.059 ± 0.159
0.818HisThr: 0.818 ± 0.119
1.338HisVal: 1.338 ± 0.15
0.297HisTrp: 0.297 ± 0.067
0.669HisTyr: 0.669 ± 0.103
0.0HisXaa: 0.0 ± 0.0
Ile
5.017IleAla: 5.017 ± 0.317
0.762IleCys: 0.762 ± 0.113
5.165IleAsp: 5.165 ± 0.217
5.407IleGlu: 5.407 ± 0.382
2.211IlePhe: 2.211 ± 0.193
3.902IleGly: 3.902 ± 0.287
1.449IleHis: 1.449 ± 0.182
4.385IleIle: 4.385 ± 0.357
6.168IleLys: 6.168 ± 0.346
4.515IleLeu: 4.515 ± 0.312
2.1IleMet: 2.1 ± 0.203
4.701IleAsn: 4.701 ± 0.365
3.251IlePro: 3.251 ± 0.2
2.434IleGln: 2.434 ± 0.237
3.976IleArg: 3.976 ± 0.26
4.385IleSer: 4.385 ± 0.319
4.626IleThr: 4.626 ± 0.294
3.976IleVal: 3.976 ± 0.271
0.892IleTrp: 0.892 ± 0.162
2.601IleTyr: 2.601 ± 0.189
0.0IleXaa: 0.0 ± 0.0
Lys
5.908LysAla: 5.908 ± 0.332
0.818LysCys: 0.818 ± 0.121
4.664LysAsp: 4.664 ± 0.256
5.295LysGlu: 5.295 ± 0.311
3.456LysPhe: 3.456 ± 0.245
3.883LysGly: 3.883 ± 0.233
1.393LysHis: 1.393 ± 0.187
5.351LysIle: 5.351 ± 0.318
4.552LysLys: 4.552 ± 0.348
5.853LysLeu: 5.853 ± 0.323
2.954LysMet: 2.954 ± 0.239
3.753LysAsn: 3.753 ± 0.222
2.527LysPro: 2.527 ± 0.223
2.397LysGln: 2.397 ± 0.22
3.251LysArg: 3.251 ± 0.289
4.608LysSer: 4.608 ± 0.312
4.088LysThr: 4.088 ± 0.26
4.682LysVal: 4.682 ± 0.337
1.393LysTrp: 1.393 ± 0.147
3.084LysTyr: 3.084 ± 0.237
0.0LysXaa: 0.0 ± 0.0
Leu
5.147LeuAla: 5.147 ± 0.332
0.985LeuCys: 0.985 ± 0.15
5.351LeuAsp: 5.351 ± 0.293
5.574LeuGlu: 5.574 ± 0.364
3.4LeuPhe: 3.4 ± 0.259
4.273LeuGly: 4.273 ± 0.317
1.208LeuHis: 1.208 ± 0.133
5.481LeuIle: 5.481 ± 0.274
5.778LeuLys: 5.778 ± 0.334
4.571LeuLeu: 4.571 ± 0.26
2.731LeuMet: 2.731 ± 0.235
4.348LeuAsn: 4.348 ± 0.274
3.214LeuPro: 3.214 ± 0.239
2.415LeuGln: 2.415 ± 0.206
3.865LeuArg: 3.865 ± 0.309
4.812LeuSer: 4.812 ± 0.269
4.311LeuThr: 4.311 ± 0.305
4.626LeuVal: 4.626 ± 0.259
0.725LeuTrp: 0.725 ± 0.125
2.583LeuTyr: 2.583 ± 0.217
0.0LeuXaa: 0.0 ± 0.0
Met
2.36MetAla: 2.36 ± 0.2
0.26MetCys: 0.26 ± 0.075
1.598MetAsp: 1.598 ± 0.193
1.877MetGlu: 1.877 ± 0.223
1.412MetPhe: 1.412 ± 0.157
1.449MetGly: 1.449 ± 0.182
0.39MetHis: 0.39 ± 0.084
2.137MetIle: 2.137 ± 0.206
3.121MetLys: 3.121 ± 0.217
2.415MetLeu: 2.415 ± 0.201
1.003MetMet: 1.003 ± 0.158
2.23MetAsn: 2.23 ± 0.182
0.687MetPro: 0.687 ± 0.117
1.189MetGln: 1.189 ± 0.146
1.152MetArg: 1.152 ± 0.154
2.007MetSer: 2.007 ± 0.19
1.988MetThr: 1.988 ± 0.204
1.524MetVal: 1.524 ± 0.146
0.242MetTrp: 0.242 ± 0.08
1.189MetTyr: 1.189 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
3.679AsnAla: 3.679 ± 0.257
0.818AsnCys: 0.818 ± 0.112
3.289AsnAsp: 3.289 ± 0.234
4.311AsnGlu: 4.311 ± 0.264
2.285AsnPhe: 2.285 ± 0.192
3.902AsnGly: 3.902 ± 0.271
0.985AsnHis: 0.985 ± 0.123
3.827AsnIle: 3.827 ± 0.245
3.604AsnLys: 3.604 ± 0.28
3.586AsnLeu: 3.586 ± 0.255
1.319AsnMet: 1.319 ± 0.165
3.251AsnAsn: 3.251 ± 0.32
2.36AsnPro: 2.36 ± 0.217
1.858AsnGln: 1.858 ± 0.181
2.62AsnArg: 2.62 ± 0.193
3.233AsnSer: 3.233 ± 0.215
2.713AsnThr: 2.713 ± 0.204
3.066AsnVal: 3.066 ± 0.226
0.576AsnTrp: 0.576 ± 0.103
2.081AsnTyr: 2.081 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
2.36ProAla: 2.36 ± 0.237
0.464ProCys: 0.464 ± 0.081
2.378ProAsp: 2.378 ± 0.208
3.196ProGlu: 3.196 ± 0.26
1.449ProPhe: 1.449 ± 0.146
2.601ProGly: 2.601 ± 0.236
0.855ProHis: 0.855 ± 0.107
2.081ProIle: 2.081 ± 0.223
2.322ProLys: 2.322 ± 0.214
2.137ProLeu: 2.137 ± 0.189
0.929ProMet: 0.929 ± 0.128
1.784ProAsn: 1.784 ± 0.171
1.078ProPro: 1.078 ± 0.164
0.743ProGln: 0.743 ± 0.111
1.431ProArg: 1.431 ± 0.145
2.174ProSer: 2.174 ± 0.188
2.062ProThr: 2.062 ± 0.195
2.564ProVal: 2.564 ± 0.222
0.65ProTrp: 0.65 ± 0.125
1.375ProTyr: 1.375 ± 0.188
0.0ProXaa: 0.0 ± 0.0
Gln
2.007GlnAla: 2.007 ± 0.239
0.26GlnCys: 0.26 ± 0.075
1.802GlnAsp: 1.802 ± 0.168
2.118GlnGlu: 2.118 ± 0.198
1.654GlnPhe: 1.654 ± 0.174
1.914GlnGly: 1.914 ± 0.168
0.539GlnHis: 0.539 ± 0.096
2.638GlnIle: 2.638 ± 0.192
2.453GlnLys: 2.453 ± 0.211
3.159GlnLeu: 3.159 ± 0.239
1.078GlnMet: 1.078 ± 0.129
1.431GlnAsn: 1.431 ± 0.165
1.189GlnPro: 1.189 ± 0.145
1.022GlnGln: 1.022 ± 0.15
1.802GlnArg: 1.802 ± 0.183
2.137GlnSer: 2.137 ± 0.199
2.248GlnThr: 2.248 ± 0.198
2.341GlnVal: 2.341 ± 0.24
0.669GlnTrp: 0.669 ± 0.112
1.672GlnTyr: 1.672 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
3.01ArgAla: 3.01 ± 0.27
0.613ArgCys: 0.613 ± 0.122
2.731ArgAsp: 2.731 ± 0.222
3.4ArgGlu: 3.4 ± 0.26
2.044ArgPhe: 2.044 ± 0.198
2.768ArgGly: 2.768 ± 0.208
0.948ArgHis: 0.948 ± 0.139
3.753ArgIle: 3.753 ± 0.256
3.214ArgLys: 3.214 ± 0.253
3.493ArgLeu: 3.493 ± 0.262
1.301ArgMet: 1.301 ± 0.121
2.285ArgAsn: 2.285 ± 0.225
1.189ArgPro: 1.189 ± 0.147
1.802ArgGln: 1.802 ± 0.196
2.211ArgArg: 2.211 ± 0.194
2.768ArgSer: 2.768 ± 0.216
2.304ArgThr: 2.304 ± 0.276
3.437ArgVal: 3.437 ± 0.262
0.706ArgTrp: 0.706 ± 0.119
1.858ArgTyr: 1.858 ± 0.174
0.0ArgXaa: 0.0 ± 0.0
Ser
3.772SerAla: 3.772 ± 0.265
0.762SerCys: 0.762 ± 0.124
3.567SerAsp: 3.567 ± 0.285
3.902SerGlu: 3.902 ± 0.243
2.824SerPhe: 2.824 ± 0.216
4.775SerGly: 4.775 ± 0.31
1.096SerHis: 1.096 ± 0.143
4.403SerIle: 4.403 ± 0.345
5.017SerLys: 5.017 ± 0.32
4.756SerLeu: 4.756 ± 0.289
1.839SerMet: 1.839 ± 0.166
3.27SerAsn: 3.27 ± 0.303
2.007SerPro: 2.007 ± 0.21
2.23SerGln: 2.23 ± 0.189
2.898SerArg: 2.898 ± 0.24
4.162SerSer: 4.162 ± 0.296
4.013SerThr: 4.013 ± 0.322
4.106SerVal: 4.106 ± 0.265
0.948SerTrp: 0.948 ± 0.13
2.583SerTyr: 2.583 ± 0.238
0.0SerXaa: 0.0 ± 0.0
Thr
3.753ThrAla: 3.753 ± 0.3
0.557ThrCys: 0.557 ± 0.109
3.01ThrAsp: 3.01 ± 0.246
3.79ThrGlu: 3.79 ± 0.293
2.62ThrPhe: 2.62 ± 0.205
4.273ThrGly: 4.273 ± 0.363
1.263ThrHis: 1.263 ± 0.153
4.273ThrIle: 4.273 ± 0.297
3.679ThrLys: 3.679 ± 0.222
4.218ThrLeu: 4.218 ± 0.28
1.301ThrMet: 1.301 ± 0.154
2.88ThrAsn: 2.88 ± 0.21
3.029ThrPro: 3.029 ± 0.266
1.895ThrGln: 1.895 ± 0.156
2.843ThrArg: 2.843 ± 0.209
3.27ThrSer: 3.27 ± 0.279
3.326ThrThr: 3.326 ± 0.265
3.53ThrVal: 3.53 ± 0.278
0.91ThrTrp: 0.91 ± 0.138
2.137ThrTyr: 2.137 ± 0.197
0.0ThrXaa: 0.0 ± 0.0
Val
4.311ValAla: 4.311 ± 0.285
0.595ValCys: 0.595 ± 0.111
4.236ValAsp: 4.236 ± 0.302
5.147ValGlu: 5.147 ± 0.306
2.583ValPhe: 2.583 ± 0.261
3.214ValGly: 3.214 ± 0.22
1.208ValHis: 1.208 ± 0.169
4.775ValIle: 4.775 ± 0.249
5.128ValLys: 5.128 ± 0.325
4.645ValLeu: 4.645 ± 0.307
1.765ValMet: 1.765 ± 0.165
3.27ValAsn: 3.27 ± 0.205
2.248ValPro: 2.248 ± 0.204
2.434ValGln: 2.434 ± 0.211
2.954ValArg: 2.954 ± 0.189
4.626ValSer: 4.626 ± 0.306
3.493ValThr: 3.493 ± 0.327
4.552ValVal: 4.552 ± 0.302
0.818ValTrp: 0.818 ± 0.098
2.75ValTyr: 2.75 ± 0.237
0.0ValXaa: 0.0 ± 0.0
Trp
1.003TrpAla: 1.003 ± 0.147
0.149TrpCys: 0.149 ± 0.054
1.003TrpAsp: 1.003 ± 0.143
1.133TrpGlu: 1.133 ± 0.163
0.669TrpPhe: 0.669 ± 0.108
0.669TrpGly: 0.669 ± 0.119
0.242TrpHis: 0.242 ± 0.078
0.929TrpIle: 0.929 ± 0.135
1.226TrpLys: 1.226 ± 0.166
1.338TrpLeu: 1.338 ± 0.142
0.464TrpMet: 0.464 ± 0.085
0.725TrpAsn: 0.725 ± 0.108
0.446TrpPro: 0.446 ± 0.094
0.595TrpGln: 0.595 ± 0.104
0.557TrpArg: 0.557 ± 0.095
0.836TrpSer: 0.836 ± 0.113
0.78TrpThr: 0.78 ± 0.132
0.948TrpVal: 0.948 ± 0.125
0.316TrpTrp: 0.316 ± 0.078
0.836TrpTyr: 0.836 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.824TyrAla: 2.824 ± 0.266
0.539TyrCys: 0.539 ± 0.084
3.14TyrAsp: 3.14 ± 0.25
2.731TyrGlu: 2.731 ± 0.23
1.839TyrPhe: 1.839 ± 0.181
2.731TyrGly: 2.731 ± 0.247
0.743TyrHis: 0.743 ± 0.114
2.936TyrIle: 2.936 ± 0.235
2.675TyrLys: 2.675 ± 0.231
2.806TyrLeu: 2.806 ± 0.242
1.152TyrMet: 1.152 ± 0.14
2.211TyrAsn: 2.211 ± 0.187
1.412TyrPro: 1.412 ± 0.142
1.579TyrGln: 1.579 ± 0.172
1.691TyrArg: 1.691 ± 0.189
2.861TyrSer: 2.861 ± 0.233
2.192TyrThr: 2.192 ± 0.198
2.657TyrVal: 2.657 ± 0.222
0.539TyrTrp: 0.539 ± 0.093
1.654TyrTyr: 1.654 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 306 proteins (53823 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski