Amino acid dipepetide frequency for Vibrio phage VP3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.951AlaAla: 8.951 ± 1.346
0.821AlaCys: 0.821 ± 0.259
4.681AlaAsp: 4.681 ± 0.69
5.748AlaGlu: 5.748 ± 1.008
2.628AlaPhe: 2.628 ± 0.456
5.584AlaGly: 5.584 ± 0.766
1.314AlaHis: 1.314 ± 0.307
5.83AlaIle: 5.83 ± 0.829
6.651AlaLys: 6.651 ± 0.768
7.144AlaLeu: 7.144 ± 0.928
3.038AlaMet: 3.038 ± 0.665
4.188AlaAsn: 4.188 ± 0.496
2.381AlaPro: 2.381 ± 0.394
3.695AlaGln: 3.695 ± 0.603
3.531AlaArg: 3.531 ± 0.505
5.009AlaSer: 5.009 ± 0.653
3.859AlaThr: 3.859 ± 0.635
5.091AlaVal: 5.091 ± 0.672
1.15AlaTrp: 1.15 ± 0.537
3.777AlaTyr: 3.777 ± 0.64
0.0AlaXaa: 0.0 ± 0.0
Cys
0.985CysAla: 0.985 ± 0.283
0.164CysCys: 0.164 ± 0.109
0.657CysAsp: 0.657 ± 0.26
0.739CysGlu: 0.739 ± 0.32
0.985CysPhe: 0.985 ± 0.266
0.821CysGly: 0.821 ± 0.257
0.493CysHis: 0.493 ± 0.175
0.246CysIle: 0.246 ± 0.139
0.575CysLys: 0.575 ± 0.2
0.821CysLeu: 0.821 ± 0.269
0.493CysMet: 0.493 ± 0.24
0.246CysAsn: 0.246 ± 0.146
0.657CysPro: 0.657 ± 0.174
0.493CysGln: 0.493 ± 0.193
0.821CysArg: 0.821 ± 0.372
0.411CysSer: 0.411 ± 0.19
0.246CysThr: 0.246 ± 0.168
0.493CysVal: 0.493 ± 0.184
0.246CysTrp: 0.246 ± 0.166
0.411CysTyr: 0.411 ± 0.206
0.0CysXaa: 0.0 ± 0.0
Asp
4.681AspAla: 4.681 ± 0.749
1.314AspCys: 1.314 ± 0.397
3.613AspAsp: 3.613 ± 0.759
4.024AspGlu: 4.024 ± 0.592
3.038AspPhe: 3.038 ± 0.537
4.598AspGly: 4.598 ± 0.527
0.739AspHis: 0.739 ± 0.233
3.859AspIle: 3.859 ± 0.522
3.613AspLys: 3.613 ± 0.567
4.106AspLeu: 4.106 ± 0.535
1.807AspMet: 1.807 ± 0.334
2.381AspAsn: 2.381 ± 0.55
2.463AspPro: 2.463 ± 0.504
1.478AspGln: 1.478 ± 0.415
2.299AspArg: 2.299 ± 0.433
3.531AspSer: 3.531 ± 0.614
4.106AspThr: 4.106 ± 0.601
4.598AspVal: 4.598 ± 0.62
0.985AspTrp: 0.985 ± 0.3
2.792AspTyr: 2.792 ± 0.616
0.0AspXaa: 0.0 ± 0.0
Glu
7.719GluAla: 7.719 ± 1.083
0.739GluCys: 0.739 ± 0.233
5.502GluAsp: 5.502 ± 0.836
5.666GluGlu: 5.666 ± 0.814
2.463GluPhe: 2.463 ± 0.31
5.009GluGly: 5.009 ± 0.799
1.478GluHis: 1.478 ± 0.442
3.942GluIle: 3.942 ± 0.665
3.367GluLys: 3.367 ± 0.447
5.994GluLeu: 5.994 ± 0.823
2.628GluMet: 2.628 ± 0.525
2.299GluAsn: 2.299 ± 0.282
1.642GluPro: 1.642 ± 0.357
3.367GluGln: 3.367 ± 0.547
3.531GluArg: 3.531 ± 0.489
4.27GluSer: 4.27 ± 0.719
3.285GluThr: 3.285 ± 0.53
4.598GluVal: 4.598 ± 0.617
1.067GluTrp: 1.067 ± 0.247
2.71GluTyr: 2.71 ± 0.406
0.0GluXaa: 0.0 ± 0.0
Phe
2.546PheAla: 2.546 ± 0.545
0.493PheCys: 0.493 ± 0.204
2.792PheAsp: 2.792 ± 0.554
3.202PheGlu: 3.202 ± 0.444
1.642PhePhe: 1.642 ± 0.513
3.285PheGly: 3.285 ± 0.549
0.575PheHis: 0.575 ± 0.247
2.71PheIle: 2.71 ± 0.471
3.367PheLys: 3.367 ± 0.661
3.449PheLeu: 3.449 ± 0.653
1.724PheMet: 1.724 ± 0.408
2.299PheAsn: 2.299 ± 0.498
1.232PhePro: 1.232 ± 0.334
1.396PheGln: 1.396 ± 0.335
1.642PheArg: 1.642 ± 0.331
2.71PheSer: 2.71 ± 0.471
2.299PheThr: 2.299 ± 0.512
2.463PheVal: 2.463 ± 0.491
0.246PheTrp: 0.246 ± 0.131
1.56PheTyr: 1.56 ± 0.333
0.0PheXaa: 0.0 ± 0.0
Gly
6.159GlyAla: 6.159 ± 0.772
0.411GlyCys: 0.411 ± 0.195
4.434GlyAsp: 4.434 ± 0.57
5.091GlyGlu: 5.091 ± 0.625
2.217GlyPhe: 2.217 ± 0.376
5.337GlyGly: 5.337 ± 0.669
1.314GlyHis: 1.314 ± 0.319
3.449GlyIle: 3.449 ± 0.545
5.173GlyLys: 5.173 ± 0.767
6.323GlyLeu: 6.323 ± 0.922
2.299GlyMet: 2.299 ± 0.47
2.956GlyAsn: 2.956 ± 0.438
0.0GlyPro: 0.0 ± 0.0
2.546GlyGln: 2.546 ± 0.424
3.777GlyArg: 3.777 ± 0.434
4.845GlySer: 4.845 ± 0.918
3.531GlyThr: 3.531 ± 0.492
4.516GlyVal: 4.516 ± 0.63
0.903GlyTrp: 0.903 ± 0.329
3.202GlyTyr: 3.202 ± 0.412
0.0GlyXaa: 0.0 ± 0.0
His
1.314HisAla: 1.314 ± 0.279
0.493HisCys: 0.493 ± 0.238
1.314HisAsp: 1.314 ± 0.409
1.56HisGlu: 1.56 ± 0.377
1.232HisPhe: 1.232 ± 0.319
1.314HisGly: 1.314 ± 0.333
0.246HisHis: 0.246 ± 0.145
1.232HisIle: 1.232 ± 0.327
0.903HisLys: 0.903 ± 0.276
1.56HisLeu: 1.56 ± 0.346
0.493HisMet: 0.493 ± 0.224
0.821HisAsn: 0.821 ± 0.249
0.493HisPro: 0.493 ± 0.179
0.246HisGln: 0.246 ± 0.133
0.903HisArg: 0.903 ± 0.309
0.657HisSer: 0.657 ± 0.318
0.739HisThr: 0.739 ± 0.305
1.478HisVal: 1.478 ± 0.268
0.493HisTrp: 0.493 ± 0.227
0.411HisTyr: 0.411 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
4.352IleAla: 4.352 ± 0.519
0.821IleCys: 0.821 ± 0.317
3.367IleAsp: 3.367 ± 0.496
4.27IleGlu: 4.27 ± 0.53
1.232IlePhe: 1.232 ± 0.373
3.531IleGly: 3.531 ± 0.514
0.821IleHis: 0.821 ± 0.264
2.792IleIle: 2.792 ± 0.626
4.763IleLys: 4.763 ± 0.665
4.681IleLeu: 4.681 ± 0.564
1.232IleMet: 1.232 ± 0.315
2.874IleAsn: 2.874 ± 0.517
2.874IlePro: 2.874 ± 0.478
2.546IleGln: 2.546 ± 0.531
3.285IleArg: 3.285 ± 0.438
3.613IleSer: 3.613 ± 0.523
3.531IleThr: 3.531 ± 0.532
3.695IleVal: 3.695 ± 0.52
0.493IleTrp: 0.493 ± 0.196
1.642IleTyr: 1.642 ± 0.308
0.0IleXaa: 0.0 ± 0.0
Lys
5.994LysAla: 5.994 ± 0.623
0.575LysCys: 0.575 ± 0.226
4.434LysAsp: 4.434 ± 0.64
3.777LysGlu: 3.777 ± 0.478
2.956LysPhe: 2.956 ± 0.48
4.598LysGly: 4.598 ± 0.774
1.15LysHis: 1.15 ± 0.289
3.038LysIle: 3.038 ± 0.492
4.598LysLys: 4.598 ± 0.739
5.584LysLeu: 5.584 ± 0.711
2.463LysMet: 2.463 ± 0.423
2.546LysAsn: 2.546 ± 0.464
2.956LysPro: 2.956 ± 0.731
3.449LysGln: 3.449 ± 0.667
3.695LysArg: 3.695 ± 0.514
3.942LysSer: 3.942 ± 0.653
4.188LysThr: 4.188 ± 0.551
4.681LysVal: 4.681 ± 0.751
1.067LysTrp: 1.067 ± 0.374
2.956LysTyr: 2.956 ± 0.53
0.0LysXaa: 0.0 ± 0.0
Leu
6.159LeuAla: 6.159 ± 0.841
0.985LeuCys: 0.985 ± 0.36
3.859LeuAsp: 3.859 ± 0.425
6.405LeuGlu: 6.405 ± 0.82
2.71LeuPhe: 2.71 ± 0.516
5.173LeuGly: 5.173 ± 0.734
1.56LeuHis: 1.56 ± 0.336
5.337LeuIle: 5.337 ± 0.808
7.555LeuLys: 7.555 ± 0.817
5.994LeuLeu: 5.994 ± 0.819
2.217LeuMet: 2.217 ± 0.348
4.845LeuAsn: 4.845 ± 0.692
2.956LeuPro: 2.956 ± 0.42
4.024LeuGln: 4.024 ± 0.549
5.255LeuArg: 5.255 ± 0.619
5.009LeuSer: 5.009 ± 0.582
5.009LeuThr: 5.009 ± 0.599
4.024LeuVal: 4.024 ± 0.524
1.232LeuTrp: 1.232 ± 0.295
2.792LeuTyr: 2.792 ± 0.472
0.0LeuXaa: 0.0 ± 0.0
Met
3.367MetAla: 3.367 ± 0.564
0.411MetCys: 0.411 ± 0.184
1.232MetAsp: 1.232 ± 0.33
2.546MetGlu: 2.546 ± 0.452
1.15MetPhe: 1.15 ± 0.339
1.971MetGly: 1.971 ± 0.605
0.411MetHis: 0.411 ± 0.175
1.478MetIle: 1.478 ± 0.297
2.217MetLys: 2.217 ± 0.415
1.724MetLeu: 1.724 ± 0.414
0.657MetMet: 0.657 ± 0.193
1.642MetAsn: 1.642 ± 0.392
1.56MetPro: 1.56 ± 0.338
1.56MetGln: 1.56 ± 0.43
1.807MetArg: 1.807 ± 0.451
1.889MetSer: 1.889 ± 0.31
2.381MetThr: 2.381 ± 0.55
1.807MetVal: 1.807 ± 0.339
0.164MetTrp: 0.164 ± 0.106
0.328MetTyr: 0.328 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
3.202AsnAla: 3.202 ± 0.537
0.328AsnCys: 0.328 ± 0.161
2.628AsnAsp: 2.628 ± 0.429
2.299AsnGlu: 2.299 ± 0.403
2.299AsnPhe: 2.299 ± 0.446
3.531AsnGly: 3.531 ± 0.647
1.232AsnHis: 1.232 ± 0.367
2.217AsnIle: 2.217 ± 0.408
2.381AsnLys: 2.381 ± 0.461
4.763AsnLeu: 4.763 ± 0.592
1.807AsnMet: 1.807 ± 0.431
1.889AsnAsn: 1.889 ± 0.31
2.874AsnPro: 2.874 ± 0.463
1.971AsnGln: 1.971 ± 0.406
2.299AsnArg: 2.299 ± 0.534
2.463AsnSer: 2.463 ± 0.545
2.381AsnThr: 2.381 ± 0.401
3.285AsnVal: 3.285 ± 0.348
0.657AsnTrp: 0.657 ± 0.254
1.724AsnTyr: 1.724 ± 0.374
0.0AsnXaa: 0.0 ± 0.0
Pro
3.202ProAla: 3.202 ± 0.57
0.328ProCys: 0.328 ± 0.169
2.463ProAsp: 2.463 ± 0.434
4.434ProGlu: 4.434 ± 0.476
1.478ProPhe: 1.478 ± 0.398
0.082ProGly: 0.082 ± 0.086
0.903ProHis: 0.903 ± 0.194
1.56ProIle: 1.56 ± 0.372
1.807ProLys: 1.807 ± 0.426
2.463ProLeu: 2.463 ± 0.393
0.903ProMet: 0.903 ± 0.28
2.546ProAsn: 2.546 ± 0.563
0.821ProPro: 0.821 ± 0.268
1.642ProGln: 1.642 ± 0.516
1.314ProArg: 1.314 ± 0.221
3.285ProSer: 3.285 ± 0.412
1.971ProThr: 1.971 ± 0.357
3.038ProVal: 3.038 ± 0.468
0.657ProTrp: 0.657 ± 0.305
1.314ProTyr: 1.314 ± 0.521
0.0ProXaa: 0.0 ± 0.0
Gln
5.091GlnAla: 5.091 ± 0.845
0.246GlnCys: 0.246 ± 0.138
2.381GlnAsp: 2.381 ± 0.455
3.613GlnGlu: 3.613 ± 0.717
1.642GlnPhe: 1.642 ± 0.398
2.299GlnGly: 2.299 ± 0.399
0.657GlnHis: 0.657 ± 0.212
2.299GlnIle: 2.299 ± 0.375
2.135GlnLys: 2.135 ± 0.485
4.27GlnLeu: 4.27 ± 0.684
0.985GlnMet: 0.985 ± 0.3
1.067GlnAsn: 1.067 ± 0.262
1.15GlnPro: 1.15 ± 0.306
2.135GlnGln: 2.135 ± 0.555
2.135GlnArg: 2.135 ± 0.352
2.956GlnSer: 2.956 ± 0.466
2.053GlnThr: 2.053 ± 0.463
3.531GlnVal: 3.531 ± 0.462
0.657GlnTrp: 0.657 ± 0.238
1.396GlnTyr: 1.396 ± 0.358
0.0GlnXaa: 0.0 ± 0.0
Arg
4.27ArgAla: 4.27 ± 0.457
0.493ArgCys: 0.493 ± 0.256
3.12ArgAsp: 3.12 ± 0.548
3.202ArgGlu: 3.202 ± 0.676
2.463ArgPhe: 2.463 ± 0.407
3.202ArgGly: 3.202 ± 0.583
0.493ArgHis: 0.493 ± 0.199
3.038ArgIle: 3.038 ± 0.477
3.531ArgLys: 3.531 ± 0.565
4.188ArgLeu: 4.188 ± 0.56
0.821ArgMet: 0.821 ± 0.294
2.463ArgAsn: 2.463 ± 0.393
2.299ArgPro: 2.299 ± 0.356
2.463ArgGln: 2.463 ± 0.434
2.463ArgArg: 2.463 ± 0.335
3.038ArgSer: 3.038 ± 0.568
2.217ArgThr: 2.217 ± 0.457
2.71ArgVal: 2.71 ± 0.504
0.657ArgTrp: 0.657 ± 0.224
2.217ArgTyr: 2.217 ± 0.433
0.0ArgXaa: 0.0 ± 0.0
Ser
4.681SerAla: 4.681 ± 0.691
0.739SerCys: 0.739 ± 0.291
4.188SerAsp: 4.188 ± 0.646
2.874SerGlu: 2.874 ± 0.491
3.285SerPhe: 3.285 ± 0.543
5.584SerGly: 5.584 ± 0.839
1.232SerHis: 1.232 ± 0.285
3.695SerIle: 3.695 ± 0.58
3.942SerLys: 3.942 ± 0.656
5.337SerLeu: 5.337 ± 0.69
1.889SerMet: 1.889 ± 0.432
2.792SerAsn: 2.792 ± 0.484
2.299SerPro: 2.299 ± 0.35
2.135SerGln: 2.135 ± 0.373
2.135SerArg: 2.135 ± 0.39
4.106SerSer: 4.106 ± 0.906
3.038SerThr: 3.038 ± 0.507
3.12SerVal: 3.12 ± 0.474
0.493SerTrp: 0.493 ± 0.171
2.628SerTyr: 2.628 ± 0.416
0.0SerXaa: 0.0 ± 0.0
Thr
3.202ThrAla: 3.202 ± 0.551
0.493ThrCys: 0.493 ± 0.185
2.71ThrAsp: 2.71 ± 0.546
3.367ThrGlu: 3.367 ± 0.523
3.777ThrPhe: 3.777 ± 0.679
5.42ThrGly: 5.42 ± 0.694
0.903ThrHis: 0.903 ± 0.218
3.695ThrIle: 3.695 ± 0.646
3.449ThrLys: 3.449 ± 0.601
5.666ThrLeu: 5.666 ± 0.763
1.396ThrMet: 1.396 ± 0.351
2.628ThrAsn: 2.628 ± 0.495
2.628ThrPro: 2.628 ± 0.51
2.628ThrGln: 2.628 ± 0.488
2.463ThrArg: 2.463 ± 0.388
2.299ThrSer: 2.299 ± 0.396
3.777ThrThr: 3.777 ± 0.55
3.12ThrVal: 3.12 ± 0.451
0.328ThrTrp: 0.328 ± 0.173
2.381ThrTyr: 2.381 ± 0.354
0.0ThrXaa: 0.0 ± 0.0
Val
5.255ValAla: 5.255 ± 0.577
0.328ValCys: 0.328 ± 0.154
3.449ValAsp: 3.449 ± 0.515
5.42ValGlu: 5.42 ± 0.692
2.135ValPhe: 2.135 ± 0.428
3.285ValGly: 3.285 ± 0.537
1.314ValHis: 1.314 ± 0.293
3.285ValIle: 3.285 ± 0.542
6.077ValLys: 6.077 ± 0.716
5.091ValLeu: 5.091 ± 0.482
1.889ValMet: 1.889 ± 0.471
3.285ValAsn: 3.285 ± 0.6
2.71ValPro: 2.71 ± 0.38
2.135ValGln: 2.135 ± 0.38
3.038ValArg: 3.038 ± 0.521
3.202ValSer: 3.202 ± 0.472
4.352ValThr: 4.352 ± 0.635
4.188ValVal: 4.188 ± 0.815
0.575ValTrp: 0.575 ± 0.161
2.874ValTyr: 2.874 ± 0.484
0.0ValXaa: 0.0 ± 0.0
Trp
0.821TrpAla: 0.821 ± 0.308
0.164TrpCys: 0.164 ± 0.102
0.739TrpAsp: 0.739 ± 0.289
0.657TrpGlu: 0.657 ± 0.197
0.575TrpPhe: 0.575 ± 0.247
0.493TrpGly: 0.493 ± 0.174
0.164TrpHis: 0.164 ± 0.123
0.657TrpIle: 0.657 ± 0.317
0.985TrpLys: 0.985 ± 0.324
1.314TrpLeu: 1.314 ± 0.412
0.164TrpMet: 0.164 ± 0.103
0.903TrpAsn: 0.903 ± 0.313
0.246TrpPro: 0.246 ± 0.159
0.493TrpGln: 0.493 ± 0.18
0.821TrpArg: 0.821 ± 0.22
0.903TrpSer: 0.903 ± 0.296
1.15TrpThr: 1.15 ± 0.298
0.821TrpVal: 0.821 ± 0.292
0.164TrpTrp: 0.164 ± 0.111
0.575TrpTyr: 0.575 ± 0.256
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.12TyrAla: 3.12 ± 0.57
0.657TyrCys: 0.657 ± 0.208
2.463TyrAsp: 2.463 ± 0.384
2.381TyrGlu: 2.381 ± 0.421
1.724TyrPhe: 1.724 ± 0.519
3.367TyrGly: 3.367 ± 0.486
0.903TyrHis: 0.903 ± 0.305
2.053TyrIle: 2.053 ± 0.486
1.56TyrLys: 1.56 ± 0.312
2.628TyrLeu: 2.628 ± 0.514
1.314TyrMet: 1.314 ± 0.309
1.56TyrAsn: 1.56 ± 0.41
1.971TyrPro: 1.971 ± 0.45
2.299TyrGln: 2.299 ± 0.398
2.217TyrArg: 2.217 ± 0.385
2.053TyrSer: 2.053 ± 0.347
2.217TyrThr: 2.217 ± 0.394
2.628TyrVal: 2.628 ± 0.532
0.575TyrTrp: 0.575 ± 0.217
0.739TyrTyr: 0.739 ± 0.333
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12179 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski