Ixcanal virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Ixcanal phlebovirus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4ZCL3|F4ZCL3_9VIRU Nucleoprotein OS=Ixcanal virus OX=1006586 GN=NP PE=3 SV=1
MM1 pKa = 7.42TSYY4 pKa = 11.29LFDD7 pKa = 3.42TPVIVRR13 pKa = 11.84PNGYY17 pKa = 9.24FDD19 pKa = 4.06RR20 pKa = 11.84EE21 pKa = 4.37VYY23 pKa = 7.9VTYY26 pKa = 9.66MAHH29 pKa = 6.83NKK31 pKa = 9.73YY32 pKa = 9.65CDD34 pKa = 3.61HH35 pKa = 7.05PVSVHH40 pKa = 5.37NCMEE44 pKa = 4.16IPVKK48 pKa = 10.93NFGVSLLHH56 pKa = 6.9KK57 pKa = 10.19EE58 pKa = 4.29SLSDD62 pKa = 4.34FYY64 pKa = 11.16ILGLLPFRR72 pKa = 11.84WGDD75 pKa = 3.04KK76 pKa = 9.48MGNSRR81 pKa = 11.84VTRR84 pKa = 11.84DD85 pKa = 3.28SFPFMRR91 pKa = 11.84DD92 pKa = 2.97LLNDD96 pKa = 3.42INDD99 pKa = 3.52MHH101 pKa = 8.0DD102 pKa = 3.48SEE104 pKa = 5.35FFISFLPNVKK114 pKa = 9.99KK115 pKa = 10.69ALSWPLGYY123 pKa = 7.63PTLDD127 pKa = 4.63FIRR130 pKa = 11.84MTRR133 pKa = 11.84SEE135 pKa = 4.11LDD137 pKa = 2.93IPHH140 pKa = 6.26FRR142 pKa = 11.84EE143 pKa = 3.72ISSNATLIMQMGQPAIDD160 pKa = 4.12LDD162 pKa = 3.78QGFVNAHH169 pKa = 5.65KK170 pKa = 10.07RR171 pKa = 11.84IVMEE175 pKa = 3.94SVTRR179 pKa = 11.84GFGHH183 pKa = 7.39KK184 pKa = 10.24DD185 pKa = 3.35FPGKK189 pKa = 10.59NMIFEE194 pKa = 4.4VASLQCVRR202 pKa = 11.84LLNAVPADD210 pKa = 3.79LLYY213 pKa = 11.13CQVTNSLTSTVMKK226 pKa = 9.65HH227 pKa = 5.96RR228 pKa = 11.84SMLEE232 pKa = 3.66DD233 pKa = 4.0HH234 pKa = 6.88PQHH237 pKa = 6.87PLGNQKK243 pKa = 7.49WTPDD247 pKa = 3.73PDD249 pKa = 3.18SWLLDD254 pKa = 3.61DD255 pKa = 5.13QAATLHH261 pKa = 6.66EE262 pKa = 4.94DD263 pKa = 3.26FALILSEE270 pKa = 5.18SDD272 pKa = 3.71DD273 pKa = 5.56DD274 pKa = 4.45EE275 pKa = 5.01DD276 pKa = 4.25

Molecular weight: 31.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4ZCL4|F4ZCL4_9VIRU Replicase OS=Ixcanal virus OX=1006586 GN=L PE=4 SV=1
MM1 pKa = 7.94ADD3 pKa = 3.97FEE5 pKa = 4.33RR6 pKa = 11.84LAIEE10 pKa = 4.29FSEE13 pKa = 4.5AGVNIADD20 pKa = 3.57VVNWVNEE27 pKa = 3.75FAYY30 pKa = 10.45QGFDD34 pKa = 2.96AKK36 pKa = 10.46RR37 pKa = 11.84VLEE40 pKa = 4.07LLQQRR45 pKa = 11.84GGSSWQEE52 pKa = 4.09DD53 pKa = 3.61ARR55 pKa = 11.84KK56 pKa = 9.14MIVLALTRR64 pKa = 11.84GNKK67 pKa = 8.15IEE69 pKa = 4.3KK70 pKa = 8.97MVLKK74 pKa = 10.19MSEE77 pKa = 4.1EE78 pKa = 4.2GKK80 pKa = 8.63KK81 pKa = 8.61TVLALKK87 pKa = 10.23KK88 pKa = 10.2KK89 pKa = 9.41YY90 pKa = 7.98QLKK93 pKa = 10.32SGNPGRR99 pKa = 11.84DD100 pKa = 3.45DD101 pKa = 3.54LTLSRR106 pKa = 11.84VAAALAGWTCQALPHH121 pKa = 6.37VEE123 pKa = 3.94NFLPVTGEE131 pKa = 3.97AMDD134 pKa = 4.37ALSPGFPRR142 pKa = 11.84CLMHH146 pKa = 7.07PSFAGLVDD154 pKa = 3.56TTLPPDD160 pKa = 3.66TQDD163 pKa = 4.43AILAAHH169 pKa = 6.45SLFLVQFSKK178 pKa = 11.17VINPNLRR185 pKa = 11.84GKK187 pKa = 9.78PKK189 pKa = 10.67GEE191 pKa = 3.8VVASFKK197 pKa = 11.0QPMLAAVNGGFIGPDD212 pKa = 2.71KK213 pKa = 10.31RR214 pKa = 11.84RR215 pKa = 11.84RR216 pKa = 11.84MLQSLGVVDD225 pKa = 4.2VNGVPSEE232 pKa = 4.39AIKK235 pKa = 10.7VAANKK240 pKa = 9.18FRR242 pKa = 11.84NSQQ245 pKa = 3.01

Molecular weight: 26.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3915

245

2085

978.8

110.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.879 ± 0.822

2.81 ± 0.976

5.875 ± 0.386

6.743 ± 0.68

4.623 ± 0.199

6.156 ± 0.287

2.452 ± 0.195

6.079 ± 0.393

6.309 ± 0.334

9.119 ± 0.622

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.116 ± 0.271

3.704 ± 0.267

3.959 ± 0.339

3.193 ± 0.166

5.594 ± 0.26

9.834 ± 0.592

4.904 ± 0.286

6.692 ± 0.235

1.303 ± 0.114

2.656 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski