Amino acid dipepetide frequency for Agrobacterium phage Atu_ph04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.969AlaAla: 3.969 ± 0.491
0.157AlaCys: 0.157 ± 0.086
3.603AlaAsp: 3.603 ± 0.456
4.387AlaGlu: 4.387 ± 0.595
2.716AlaPhe: 2.716 ± 0.374
4.387AlaGly: 4.387 ± 0.635
0.731AlaHis: 0.731 ± 0.184
5.013AlaIle: 5.013 ± 0.564
5.327AlaLys: 5.327 ± 0.591
5.118AlaLeu: 5.118 ± 0.648
1.462AlaMet: 1.462 ± 0.27
3.29AlaAsn: 3.29 ± 0.423
2.193AlaPro: 2.193 ± 0.294
2.35AlaGln: 2.35 ± 0.484
3.812AlaArg: 3.812 ± 0.404
4.439AlaSer: 4.439 ± 0.53
2.454AlaThr: 2.454 ± 0.368
4.648AlaVal: 4.648 ± 0.602
1.044AlaTrp: 1.044 ± 0.217
2.037AlaTyr: 2.037 ± 0.329
0.0AlaXaa: 0.0 ± 0.0
Cys
0.366CysAla: 0.366 ± 0.131
0.0CysCys: 0.0 ± 0.0
0.418CysAsp: 0.418 ± 0.16
0.836CysGlu: 0.836 ± 0.177
0.261CysPhe: 0.261 ± 0.111
0.522CysGly: 0.522 ± 0.161
0.261CysHis: 0.261 ± 0.117
0.47CysIle: 0.47 ± 0.131
0.522CysLys: 0.522 ± 0.178
0.522CysLeu: 0.522 ± 0.133
0.313CysMet: 0.313 ± 0.113
0.261CysAsn: 0.261 ± 0.121
0.261CysPro: 0.261 ± 0.118
0.418CysGln: 0.418 ± 0.184
0.574CysArg: 0.574 ± 0.185
0.47CysSer: 0.47 ± 0.154
0.261CysThr: 0.261 ± 0.128
0.522CysVal: 0.522 ± 0.162
0.104CysTrp: 0.104 ± 0.078
0.313CysTyr: 0.313 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
3.603AspAla: 3.603 ± 0.365
0.313AspCys: 0.313 ± 0.117
4.961AspAsp: 4.961 ± 0.629
5.379AspGlu: 5.379 ± 0.629
3.917AspPhe: 3.917 ± 0.47
4.387AspGly: 4.387 ± 0.45
0.992AspHis: 0.992 ± 0.235
5.118AspIle: 5.118 ± 0.594
4.961AspLys: 4.961 ± 0.556
5.066AspLeu: 5.066 ± 0.491
1.41AspMet: 1.41 ± 0.25
2.663AspAsn: 2.663 ± 0.367
2.768AspPro: 2.768 ± 0.39
2.089AspGln: 2.089 ± 0.259
3.238AspArg: 3.238 ± 0.436
4.596AspSer: 4.596 ± 0.626
3.812AspThr: 3.812 ± 0.491
4.596AspVal: 4.596 ± 0.452
0.627AspTrp: 0.627 ± 0.18
2.454AspTyr: 2.454 ± 0.35
0.0AspXaa: 0.0 ± 0.0
Glu
5.536GluAla: 5.536 ± 0.503
0.574GluCys: 0.574 ± 0.165
4.178GluAsp: 4.178 ± 0.585
5.483GluGlu: 5.483 ± 1.174
4.073GluPhe: 4.073 ± 0.462
3.551GluGly: 3.551 ± 0.42
1.462GluHis: 1.462 ± 0.359
6.789GluIle: 6.789 ± 0.555
5.797GluLys: 5.797 ± 1.002
5.953GluLeu: 5.953 ± 0.658
1.723GluMet: 1.723 ± 0.341
4.387GluAsn: 4.387 ± 0.52
2.193GluPro: 2.193 ± 0.38
1.984GluGln: 1.984 ± 0.33
3.708GluArg: 3.708 ± 0.452
4.178GluSer: 4.178 ± 0.658
4.648GluThr: 4.648 ± 0.525
4.178GluVal: 4.178 ± 0.554
0.888GluTrp: 0.888 ± 0.244
2.402GluTyr: 2.402 ± 0.339
0.0GluXaa: 0.0 ± 0.0
Phe
3.186PheAla: 3.186 ± 0.386
0.366PheCys: 0.366 ± 0.148
4.491PheAsp: 4.491 ± 0.556
4.126PheGlu: 4.126 ± 0.536
2.454PhePhe: 2.454 ± 0.396
3.447PheGly: 3.447 ± 0.335
0.783PheHis: 0.783 ± 0.221
2.768PheIle: 2.768 ± 0.329
3.29PheLys: 3.29 ± 0.396
3.186PheLeu: 3.186 ± 0.436
1.462PheMet: 1.462 ± 0.323
2.82PheAsn: 2.82 ± 0.403
1.306PhePro: 1.306 ± 0.319
1.462PheGln: 1.462 ± 0.275
3.029PheArg: 3.029 ± 0.407
4.23PheSer: 4.23 ± 0.532
2.402PheThr: 2.402 ± 0.468
3.603PheVal: 3.603 ± 0.383
0.888PheTrp: 0.888 ± 0.258
1.671PheTyr: 1.671 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
3.29GlyAla: 3.29 ± 0.606
0.679GlyCys: 0.679 ± 0.201
3.969GlyAsp: 3.969 ± 0.499
4.334GlyGlu: 4.334 ± 0.614
2.507GlyPhe: 2.507 ± 0.322
4.909GlyGly: 4.909 ± 1.166
1.514GlyHis: 1.514 ± 0.357
4.543GlyIle: 4.543 ± 0.458
5.066GlyLys: 5.066 ± 0.635
4.857GlyLeu: 4.857 ± 0.421
1.567GlyMet: 1.567 ± 0.243
4.282GlyAsn: 4.282 ± 0.837
1.41GlyPro: 1.41 ± 0.276
1.358GlyGln: 1.358 ± 0.305
2.82GlyArg: 2.82 ± 0.382
6.162GlySer: 6.162 ± 0.878
4.439GlyThr: 4.439 ± 0.754
4.909GlyVal: 4.909 ± 0.592
0.627GlyTrp: 0.627 ± 0.167
2.35GlyTyr: 2.35 ± 0.346
0.0GlyXaa: 0.0 ± 0.0
His
1.149HisAla: 1.149 ± 0.268
0.261HisCys: 0.261 ± 0.108
1.097HisAsp: 1.097 ± 0.277
0.94HisGlu: 0.94 ± 0.256
1.41HisPhe: 1.41 ± 0.288
1.358HisGly: 1.358 ± 0.323
0.574HisHis: 0.574 ± 0.18
1.358HisIle: 1.358 ± 0.286
1.097HisLys: 1.097 ± 0.284
1.462HisLeu: 1.462 ± 0.332
0.261HisMet: 0.261 ± 0.103
0.679HisAsn: 0.679 ± 0.223
0.574HisPro: 0.574 ± 0.179
0.313HisGln: 0.313 ± 0.158
0.94HisArg: 0.94 ± 0.22
1.253HisSer: 1.253 ± 0.261
0.522HisThr: 0.522 ± 0.154
1.567HisVal: 1.567 ± 0.341
0.104HisTrp: 0.104 ± 0.071
0.574HisTyr: 0.574 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
5.222IleAla: 5.222 ± 0.518
0.679IleCys: 0.679 ± 0.149
6.267IleAsp: 6.267 ± 0.732
5.066IleGlu: 5.066 ± 0.601
3.186IlePhe: 3.186 ± 0.424
3.656IleGly: 3.656 ± 0.493
1.044IleHis: 1.044 ± 0.226
3.238IleIle: 3.238 ± 0.499
3.917IleLys: 3.917 ± 0.454
4.178IleLeu: 4.178 ± 0.385
0.992IleMet: 0.992 ± 0.208
3.29IleAsn: 3.29 ± 0.528
3.29IlePro: 3.29 ± 0.401
1.932IleGln: 1.932 ± 0.426
3.656IleArg: 3.656 ± 0.476
5.222IleSer: 5.222 ± 0.639
3.342IleThr: 3.342 ± 0.578
5.692IleVal: 5.692 ± 0.548
0.522IleTrp: 0.522 ± 0.16
2.716IleTyr: 2.716 ± 0.33
0.0IleXaa: 0.0 ± 0.0
Lys
4.961LysAla: 4.961 ± 0.583
0.574LysCys: 0.574 ± 0.165
4.126LysAsp: 4.126 ± 0.48
5.222LysGlu: 5.222 ± 0.614
4.282LysPhe: 4.282 ± 0.544
2.977LysGly: 2.977 ± 0.444
1.201LysHis: 1.201 ± 0.341
6.267LysIle: 6.267 ± 0.658
5.692LysLys: 5.692 ± 1.1
4.596LysLeu: 4.596 ± 0.669
1.358LysMet: 1.358 ± 0.321
4.073LysAsn: 4.073 ± 0.403
1.88LysPro: 1.88 ± 0.291
1.984LysGln: 1.984 ± 0.322
3.186LysArg: 3.186 ± 0.423
5.744LysSer: 5.744 ± 0.665
4.7LysThr: 4.7 ± 0.503
4.073LysVal: 4.073 ± 0.503
0.731LysTrp: 0.731 ± 0.204
2.089LysTyr: 2.089 ± 0.334
0.0LysXaa: 0.0 ± 0.0
Leu
5.013LeuAla: 5.013 ± 0.542
0.627LeuCys: 0.627 ± 0.204
4.961LeuAsp: 4.961 ± 0.415
5.744LeuGlu: 5.744 ± 0.645
3.603LeuPhe: 3.603 ± 0.36
4.752LeuGly: 4.752 ± 0.435
1.306LeuHis: 1.306 ± 0.263
3.812LeuIle: 3.812 ± 0.484
5.536LeuLys: 5.536 ± 0.594
5.327LeuLeu: 5.327 ± 0.633
1.828LeuMet: 1.828 ± 0.34
4.073LeuAsn: 4.073 ± 0.453
3.081LeuPro: 3.081 ± 0.397
2.141LeuGln: 2.141 ± 0.276
4.491LeuArg: 4.491 ± 0.463
6.11LeuSer: 6.11 ± 0.555
4.596LeuThr: 4.596 ± 0.507
5.64LeuVal: 5.64 ± 0.557
0.574LeuTrp: 0.574 ± 0.191
2.193LeuTyr: 2.193 ± 0.338
0.0LeuXaa: 0.0 ± 0.0
Met
1.828MetAla: 1.828 ± 0.259
0.157MetCys: 0.157 ± 0.1
0.783MetAsp: 0.783 ± 0.258
1.41MetGlu: 1.41 ± 0.326
0.992MetPhe: 0.992 ± 0.252
1.097MetGly: 1.097 ± 0.242
0.366MetHis: 0.366 ± 0.141
1.41MetIle: 1.41 ± 0.275
1.567MetLys: 1.567 ± 0.339
1.932MetLeu: 1.932 ± 0.285
0.836MetMet: 0.836 ± 0.264
1.097MetAsn: 1.097 ± 0.261
0.574MetPro: 0.574 ± 0.137
0.679MetGln: 0.679 ± 0.188
1.41MetArg: 1.41 ± 0.273
1.253MetSer: 1.253 ± 0.284
2.089MetThr: 2.089 ± 0.263
1.149MetVal: 1.149 ± 0.254
0.157MetTrp: 0.157 ± 0.085
0.574MetTyr: 0.574 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
3.29AsnAla: 3.29 ± 0.429
0.261AsnCys: 0.261 ± 0.136
2.507AsnAsp: 2.507 ± 0.33
3.917AsnGlu: 3.917 ± 0.561
3.342AsnPhe: 3.342 ± 0.413
5.901AsnGly: 5.901 ± 0.933
0.366AsnHis: 0.366 ± 0.132
2.611AsnIle: 2.611 ± 0.505
2.402AsnLys: 2.402 ± 0.383
4.282AsnLeu: 4.282 ± 0.58
1.462AsnMet: 1.462 ± 0.287
2.663AsnAsn: 2.663 ± 0.41
3.394AsnPro: 3.394 ± 0.523
1.932AsnGln: 1.932 ± 0.284
2.246AsnArg: 2.246 ± 0.368
4.334AsnSer: 4.334 ± 0.531
2.768AsnThr: 2.768 ± 0.298
4.23AsnVal: 4.23 ± 0.474
0.366AsnTrp: 0.366 ± 0.156
2.141AsnTyr: 2.141 ± 0.327
0.0AsnXaa: 0.0 ± 0.0
Pro
1.828ProAla: 1.828 ± 0.423
0.261ProCys: 0.261 ± 0.115
2.402ProAsp: 2.402 ± 0.365
2.559ProGlu: 2.559 ± 0.369
1.514ProPhe: 1.514 ± 0.274
3.081ProGly: 3.081 ± 0.521
0.574ProHis: 0.574 ± 0.161
1.984ProIle: 1.984 ± 0.349
2.559ProLys: 2.559 ± 0.393
3.342ProLeu: 3.342 ± 0.452
0.313ProMet: 0.313 ± 0.117
1.671ProAsn: 1.671 ± 0.337
0.836ProPro: 0.836 ± 0.206
0.992ProGln: 0.992 ± 0.191
1.671ProArg: 1.671 ± 0.278
3.864ProSer: 3.864 ± 0.521
1.828ProThr: 1.828 ± 0.275
2.611ProVal: 2.611 ± 0.482
0.522ProTrp: 0.522 ± 0.202
1.619ProTyr: 1.619 ± 0.236
0.0ProXaa: 0.0 ± 0.0
Gln
1.723GlnAla: 1.723 ± 0.31
0.366GlnCys: 0.366 ± 0.148
1.462GlnAsp: 1.462 ± 0.289
1.984GlnGlu: 1.984 ± 0.329
1.723GlnPhe: 1.723 ± 0.342
1.201GlnGly: 1.201 ± 0.244
0.47GlnHis: 0.47 ± 0.145
2.559GlnIle: 2.559 ± 0.317
2.246GlnLys: 2.246 ± 0.378
2.716GlnLeu: 2.716 ± 0.395
0.731GlnMet: 0.731 ± 0.22
1.984GlnAsn: 1.984 ± 0.273
0.992GlnPro: 0.992 ± 0.25
0.679GlnGln: 0.679 ± 0.186
1.306GlnArg: 1.306 ± 0.332
2.35GlnSer: 2.35 ± 0.377
1.88GlnThr: 1.88 ± 0.341
1.776GlnVal: 1.776 ± 0.309
0.366GlnTrp: 0.366 ± 0.159
0.888GlnTyr: 0.888 ± 0.194
0.0GlnXaa: 0.0 ± 0.0
Arg
2.193ArgAla: 2.193 ± 0.41
0.418ArgCys: 0.418 ± 0.139
3.708ArgAsp: 3.708 ± 0.499
3.969ArgGlu: 3.969 ± 0.443
3.29ArgPhe: 3.29 ± 0.388
3.081ArgGly: 3.081 ± 0.377
0.992ArgHis: 0.992 ± 0.301
3.917ArgIle: 3.917 ± 0.447
3.342ArgLys: 3.342 ± 0.453
4.334ArgLeu: 4.334 ± 0.621
0.888ArgMet: 0.888 ± 0.229
3.656ArgAsn: 3.656 ± 0.467
2.037ArgPro: 2.037 ± 0.449
1.567ArgGln: 1.567 ± 0.332
2.246ArgArg: 2.246 ± 0.296
3.186ArgSer: 3.186 ± 0.401
2.193ArgThr: 2.193 ± 0.273
4.073ArgVal: 4.073 ± 0.421
0.313ArgTrp: 0.313 ± 0.129
2.559ArgTyr: 2.559 ± 0.314
0.0ArgXaa: 0.0 ± 0.0
Ser
5.431SerAla: 5.431 ± 0.855
0.418SerCys: 0.418 ± 0.203
5.118SerAsp: 5.118 ± 0.529
6.319SerGlu: 6.319 ± 0.66
3.342SerPhe: 3.342 ± 0.393
6.058SerGly: 6.058 ± 0.993
1.044SerHis: 1.044 ± 0.26
5.431SerIle: 5.431 ± 0.501
5.222SerLys: 5.222 ± 0.561
5.327SerLeu: 5.327 ± 0.468
1.828SerMet: 1.828 ± 0.366
4.073SerAsn: 4.073 ± 0.562
2.193SerPro: 2.193 ± 0.408
2.193SerGln: 2.193 ± 0.329
3.447SerArg: 3.447 ± 0.509
6.214SerSer: 6.214 ± 1.092
4.334SerThr: 4.334 ± 0.616
5.222SerVal: 5.222 ± 0.771
0.783SerTrp: 0.783 ± 0.194
2.559SerTyr: 2.559 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
3.29ThrAla: 3.29 ± 0.403
0.104ThrCys: 0.104 ± 0.076
3.708ThrAsp: 3.708 ± 0.583
4.387ThrGlu: 4.387 ± 0.416
2.402ThrPhe: 2.402 ± 0.346
4.648ThrGly: 4.648 ± 0.64
0.627ThrHis: 0.627 ± 0.168
4.334ThrIle: 4.334 ± 0.599
3.186ThrLys: 3.186 ± 0.518
4.648ThrLeu: 4.648 ± 0.6
1.044ThrMet: 1.044 ± 0.242
2.663ThrAsn: 2.663 ± 0.308
2.298ThrPro: 2.298 ± 0.32
1.723ThrGln: 1.723 ± 0.331
2.872ThrArg: 2.872 ± 0.348
4.387ThrSer: 4.387 ± 0.912
3.394ThrThr: 3.394 ± 0.736
4.334ThrVal: 4.334 ± 0.537
0.731ThrTrp: 0.731 ± 0.17
1.984ThrTyr: 1.984 ± 0.374
0.0ThrXaa: 0.0 ± 0.0
Val
4.073ValAla: 4.073 ± 0.463
0.888ValCys: 0.888 ± 0.209
6.267ValAsp: 6.267 ± 0.624
4.961ValGlu: 4.961 ± 0.653
3.29ValPhe: 3.29 ± 0.404
4.178ValGly: 4.178 ± 0.559
2.037ValHis: 2.037 ± 0.323
3.499ValIle: 3.499 ± 0.546
4.804ValLys: 4.804 ± 0.439
4.7ValLeu: 4.7 ± 0.468
0.836ValMet: 0.836 ± 0.204
4.178ValAsn: 4.178 ± 0.475
3.133ValPro: 3.133 ± 0.428
2.35ValGln: 2.35 ± 0.284
4.596ValArg: 4.596 ± 0.45
5.431ValSer: 5.431 ± 0.599
4.126ValThr: 4.126 ± 0.801
4.752ValVal: 4.752 ± 0.716
0.783ValTrp: 0.783 ± 0.176
2.089ValTyr: 2.089 ± 0.325
0.0ValXaa: 0.0 ± 0.0
Trp
0.47TrpAla: 0.47 ± 0.145
0.313TrpCys: 0.313 ± 0.144
0.627TrpAsp: 0.627 ± 0.189
0.574TrpGlu: 0.574 ± 0.192
0.627TrpPhe: 0.627 ± 0.185
0.418TrpGly: 0.418 ± 0.179
0.522TrpHis: 0.522 ± 0.154
0.418TrpIle: 0.418 ± 0.123
0.679TrpLys: 0.679 ± 0.144
1.149TrpLeu: 1.149 ± 0.286
0.522TrpMet: 0.522 ± 0.167
0.94TrpAsn: 0.94 ± 0.232
0.313TrpPro: 0.313 ± 0.199
0.209TrpGln: 0.209 ± 0.096
0.888TrpArg: 0.888 ± 0.206
0.522TrpSer: 0.522 ± 0.154
0.366TrpThr: 0.366 ± 0.103
0.574TrpVal: 0.574 ± 0.155
0.052TrpTrp: 0.052 ± 0.056
0.313TrpTyr: 0.313 ± 0.103
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.716TyrAla: 2.716 ± 0.322
0.261TyrCys: 0.261 ± 0.109
2.298TyrAsp: 2.298 ± 0.41
2.089TyrGlu: 2.089 ± 0.291
2.037TyrPhe: 2.037 ± 0.358
1.984TyrGly: 1.984 ± 0.256
0.627TyrHis: 0.627 ± 0.2
1.567TyrIle: 1.567 ± 0.324
2.507TyrLys: 2.507 ± 0.399
2.716TyrLeu: 2.716 ± 0.37
0.47TyrMet: 0.47 ± 0.147
1.671TyrAsn: 1.671 ± 0.321
1.306TyrPro: 1.306 ± 0.243
1.044TyrGln: 1.044 ± 0.196
1.828TyrArg: 1.828 ± 0.288
2.663TyrSer: 2.663 ± 0.385
2.611TyrThr: 2.611 ± 0.494
2.82TyrVal: 2.82 ± 0.478
0.313TyrTrp: 0.313 ± 0.115
0.783TyrTyr: 0.783 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (19150 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski